Restriction map for
pF1KE1068
(Linear) (Six-base) MAPSORT of: KIEE1068.seq Check: 2955 from: 1 to: 1731
KIEE1068.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 0 83 1401 1444 1731
Size: 83 1318 43 287
Fragments arranged by size:
1318 287 83 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 436 689 1099 1135 1706 1731
Size: 125 311 253 410 36 571 25
Fragments arranged by size:
571 410 311 253 125 36 25
AfeI AGC'GCT
Cuts at: 0 1588 1731
Size: 1588 143
AflII C'TTAA_G
Cuts at: 0 160 892 1731
Size: 160 732 839
Fragments arranged by size:
839 732 160
AflIII A'CryG_T
Cuts at: 0 802 1396 1731
Size: 802 594 335
Fragments arranged by size:
802 594 335
AlwNI CAG_nnn'CTG
Cuts at: 0 1107 1158 1557 1710 1731
Size: 1107 51 399 153 21
Fragments arranged by size:
1107 399 153 51 21
ApaI G_GGCC'C
Cuts at: 0 19 1731
Size: 19 1712
ApoI r'AATT_y
Cuts at: 0 95 237 1731
Size: 95 142 1494
Fragments arranged by size:
1494 142 95
BanII G_rGCy'C
Cuts at: 0 19 1564 1731
Size: 19 1545 167
Fragments arranged by size:
1545 167 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 296 1339 1731
Size: 296 1043 392
Fragments arranged by size:
1043 392 296
BbvCI CC'TCA_GC
Cuts at: 0 1583 1731
Size: 1583 148
BciVI GTATCCnnnnn_n'
Cuts at: 0 588 1731
Size: 588 1143
BclI T'GATC_A
Cuts at: 0 428 1731
Size: 428 1303
BglII A'GATC_T
Cuts at: 0 1082 1731
Size: 1082 649
BlpI GC'TnA_GC
Cuts at: 0 374 1731
Size: 374 1357
Bme1580I G_kGCm'C
Cuts at: 0 19 1053 1731
Size: 19 1034 678
Fragments arranged by size:
1034 678 19
BmgBI CAC'GTC
Cuts at: 0 9 1731
Size: 9 1722
BmrI ACTGGGnnnn_n'
Cuts at: 0 23 1365 1731
Size: 23 1342 366
Fragments arranged by size:
1342 366 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 583 803 1581 1731
Size: 66 517 220 778 150
Fragments arranged by size:
778 517 220 150 66
Bpu10I CC'TnA_GC
Cuts at: 0 1583 1731
Size: 1583 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1731
Size: 654 1077
BsaI GGTCTCn'nnnn_
Cuts at: 0 787 1731
Size: 787 944
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1501 1531 1731
Size: 1501 30 200
Fragments arranged by size:
1501 200 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1711 1731
Size: 1711 20
BseYI C'CCAG_C
Cuts at: 0 1166 1606 1731
Size: 1166 440 125
Fragments arranged by size:
1166 440 125
BsmI GAATG_Cn'
Cuts at: 0 475 566 1731
Size: 475 91 1165
Fragments arranged by size:
1165 475 91
Bsp1286I G_dGCh'C
Cuts at: 0 19 1053 1564 1731
Size: 19 1034 511 167
Fragments arranged by size:
1034 511 167 19
BspHI T'CATG_A
Cuts at: 0 945 1731
Size: 945 786
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 564 840 1731
Size: 564 276 891
Fragments arranged by size:
891 564 276
BsrDI GCAATG_nn'
Cuts at: 0 302 1731
Size: 302 1429
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1110 1731
Size: 1110 621
BstYI r'GATC_y
Cuts at: 0 1082 1731
Size: 1082 649
BstZ17I GTA'TAC
Cuts at: 0 84 1731
Size: 84 1647
BtgI C'CryG_G
Cuts at: 0 35 194 1731
Size: 35 159 1537
Fragments arranged by size:
1537 159 35
BtsI GCAGTG_nn'
Cuts at: 0 1051 1731
Size: 1051 680
EaeI y'GGCC_r
Cuts at: 0 326 1731
Size: 326 1405
EarI CTCTTCn'nnn_
Cuts at: 0 455 1682 1731
Size: 455 1227 49
Fragments arranged by size:
1227 455 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1731
Size: 19 1712
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 125 436 583 689 803 1099 1135
Size: 66 59 311 147 106 114 296 36
Cuts at: 1135 1581 1706 1731
Size: 446 125 25
Fragments arranged by size:
446 311 296 147 125 114 106 66
59 36 25
EcoO109I rG'GnC_Cy
Cuts at: 0 16 1610 1731
Size: 16 1594 121
Fragments arranged by size:
1594 121 16
EcoRI G'AATT_C
Cuts at: 0 237 1731
Size: 237 1494
HaeII r_GCGC'y
Cuts at: 0 1149 1590 1731
Size: 1149 441 141
Fragments arranged by size:
1149 441 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 367 399 645 677 1731
Size: 367 32 246 32 1054
Fragments arranged by size:
1054 367 246 32 32
HindIII A'AGCT_T
Cuts at: 0 1180 1731
Size: 1180 551
MfeI C'AATT_G
Cuts at: 0 1227 1731
Size: 1227 504
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 1520 1656 1673 1731
Size: 389 1131 136 17 58
Fragments arranged by size:
1131 389 136 58 17
MscI TGG'CCA
Cuts at: 0 328 1731
Size: 328 1403
MspA1I CmG'CkG
Cuts at: 0 37 1731
Size: 37 1694
NcoI C'CATG_G
Cuts at: 0 194 1731
Size: 194 1537
NdeI CA'TA_TG
Cuts at: 0 1355 1731
Size: 1355 376
NspI r_CATG'y
Cuts at: 0 806 1400 1731
Size: 806 594 331
Fragments arranged by size:
806 594 331
PacI TTA_AT'TAA
Cuts at: 0 317 1731
Size: 317 1414
PciI A'CATG_T
Cuts at: 0 802 1396 1731
Size: 802 594 335
Fragments arranged by size:
802 594 335
PflMI CCAn_nnn'nTGG
Cuts at: 0 1309 1731
Size: 1309 422
PfoI T'CCnGG_A
Cuts at: 0 90 692 1731
Size: 90 602 1039
Fragments arranged by size:
1039 602 90
PshAI GACnn'nnGTC
Cuts at: 0 681 1731
Size: 681 1050
PspOMI G'GGCC_C
Cuts at: 0 15 1731
Size: 15 1716
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 71 103 1731
Size: 71 32 1628
Fragments arranged by size:
1628 71 32
PstI C_TGCA'G
Cuts at: 0 247 690 837 936 1731
Size: 247 443 147 99 795
Fragments arranged by size:
795 443 247 147 99
SacII CC_GC'GG
Cuts at: 0 38 1731
Size: 38 1693
SapI GCTCTTCn'nnn_
Cuts at: 0 455 1731
Size: 455 1276
SbfI CC_TGCA'GG
Cuts at: 0 247 1731
Size: 247 1484
SfcI C'TryA_G
Cuts at: 0 243 457 686 833 932 1731
Size: 243 214 229 147 99 799
Fragments arranged by size:
799 243 229 214 147 99
SmlI C'TyrA_G
Cuts at: 0 160 633 892 1731
Size: 160 473 259 839
Fragments arranged by size:
839 473 259 160
SspI AAT'ATT
Cuts at: 0 502 1731
Size: 502 1229
StyI C'CwwG_G
Cuts at: 0 194 1731
Size: 194 1537
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1501 1533 1731
Size: 1501 32 198
Fragments arranged by size:
1501 198 32
Tth111I GACn'n_nGTC
Cuts at: 0 1637 1731
Size: 1637 94
XbaI T'CTAG_A
Cuts at: 0 774 1731
Size: 774 957
XmnI GAAnn'nnTTC
Cuts at: 0 449 1731
Size: 449 1282
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AlwNI ApaI ApoI
BanII BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI
BseYI BsmI Bsp1286I BspHI BspMI BsrDI BstAPI BstYI
BstZ17I BtgI BtsI EaeI EarI EciI Eco57MI EcoO109I
EcoRI HaeII Hin4I HindIII MfeI MmeI MscI MspA1I
NcoI NdeI NspI PacI PciI PflMI PfoI PshAI
PspOMI PsrI PstI SacII SapI SbfI SfcI SmlI
SspI StyI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BcgI BfrBI BglI BmtI BplI
BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HincII HpaI KasI KpnI MluI MslI NaeI
NarI NgoMIV NheI NotI NruI NsiI PasI PmeI
PmlI PpiI PpuMI PsiI PspXI PvuI PvuII RsrII
SacI SalI SanDI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TaqII TatI XcmI XhoI XmaI ZraI