Restriction map for
pF1KE1071
(Linear) (Six-base) MAPSORT of: KIEE1071.seq Check: 1596 from: 1 to: 2295
KIEE1071.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
Acc65I G'GTAC_C
Cuts at: 0 153 2295
Size: 153 2142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 2295
Size: 470 1825
AlwNI CAG_nnn'CTG
Cuts at: 0 1249 1374 2295
Size: 1249 125 921
Fragments arranged by size:
1249 921 125
ApaI G_GGCC'C
Cuts at: 0 249 2295
Size: 249 2046
ApaLI G'TGCA_C
Cuts at: 0 883 2295
Size: 883 1412
ApoI r'AATT_y
Cuts at: 0 374 417 782 2139 2295
Size: 374 43 365 1357 156
Fragments arranged by size:
1357 374 365 156 43
AvaI C'yCGr_G
Cuts at: 0 201 309 2295
Size: 201 108 1986
Fragments arranged by size:
1986 201 108
BanI G'GyrC_C
Cuts at: 0 153 2295
Size: 153 2142
BanII G_rGCy'C
Cuts at: 0 249 2295
Size: 249 2046
BbvCI CC'TCA_GC
Cuts at: 0 218 2295
Size: 218 2077
BciVI GTATCCnnnnn_n'
Cuts at: 0 585 2295
Size: 585 1710
BglII A'GATC_T
Cuts at: 0 348 2295
Size: 348 1947
Bme1580I G_kGCm'C
Cuts at: 0 249 887 2102 2295
Size: 249 638 1215 193
Fragments arranged by size:
1215 638 249 193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 125 1760 2295
Size: 104 21 1635 535
Fragments arranged by size:
1635 535 104 21
Bpu10I CC'TnA_GC
Cuts at: 0 218 277 2295
Size: 218 59 2018
Fragments arranged by size:
2018 218 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1103 1378 2295
Size: 1103 275 917
Fragments arranged by size:
1103 917 275
BsaI GGTCTCn'nnnn_
Cuts at: 0 728 2295
Size: 728 1567
BsaBI GATnn'nnATC
Cuts at: 0 836 1010 2295
Size: 836 174 1285
Fragments arranged by size:
1285 836 174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1758 1788 2295
Size: 1758 30 507
Fragments arranged by size:
1758 507 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 32 246 2295
Size: 32 214 2049
Fragments arranged by size:
2049 214 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1994 2295
Size: 1994 301
BsiHKAI G_wGCw'C
Cuts at: 0 887 2295
Size: 887 1408
BsmI GAATG_Cn'
Cuts at: 0 977 2295
Size: 977 1318
Bsp1286I G_dGCh'C
Cuts at: 0 249 887 2102 2295
Size: 249 638 1215 193
Fragments arranged by size:
1215 638 249 193
BsrBI CCG'CTC
Cuts at: 0 1859 2295
Size: 1859 436
BsrDI GCAATG_nn'
Cuts at: 0 511 639 841 1731 2295
Size: 511 128 202 890 564
Fragments arranged by size:
890 564 511 202 128
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1876 2295
Size: 1876 419
BstYI r'GATC_y
Cuts at: 0 348 948 1887 2295
Size: 348 600 939 408
Fragments arranged by size:
939 600 408 348
BtgI C'CryG_G
Cuts at: 0 193 1615 2295
Size: 193 1422 680
Fragments arranged by size:
1422 680 193
BtsI GCAGTG_nn'
Cuts at: 0 2102 2295
Size: 2102 193
DraI TTT'AAA
Cuts at: 0 872 2295
Size: 872 1423
DraIII CAC_nnn'GTG
Cuts at: 0 228 2295
Size: 228 2067
EaeI y'GGCC_r
Cuts at: 0 1618 2295
Size: 1618 677
EarI CTCTTCn'nnn_
Cuts at: 0 248 1379 1705 1925 2295
Size: 248 1131 326 220 370
Fragments arranged by size:
1131 370 326 248 220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1677 2295
Size: 1677 618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 125 470 1760 2295
Size: 104 21 345 1290 535
Fragments arranged by size:
1290 535 345 104 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 253 2295
Size: 253 2042
EcoO109I rG'GnC_Cy
Cuts at: 0 131 246 2295
Size: 131 115 2049
Fragments arranged by size:
2049 131 115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 367 2295
Size: 367 1928
HaeII r_GCGC'y
Cuts at: 0 2251 2295
Size: 2251 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 940 972 1132 1164 1173 1205 1455 1487
Size: 940 32 160 32 9 32 250 32
Cuts at: 1487 2295
Size: 808
Fragments arranged by size:
940 808 250 160 32 32 32 32
9
HincII GTy'rAC
Cuts at: 0 476 1221 1766 2295
Size: 476 745 545 529
Fragments arranged by size:
745 545 529 476
HpaI GTT'AAC
Cuts at: 0 476 2295
Size: 476 1819
KpnI G_GTAC'C
Cuts at: 0 157 2295
Size: 157 2138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 2295
Size: 271 2024
MscI TGG'CCA
Cuts at: 0 1620 2295
Size: 1620 675
MslI CAynn'nnrTG
Cuts at: 0 864 2295
Size: 864 1431
MspA1I CmG'CkG
Cuts at: 0 317 2295
Size: 317 1978
NspI r_CATG'y
Cuts at: 0 917 2295
Size: 917 1378
PflMI CCAn_nnn'nTGG
Cuts at: 0 660 2295
Size: 660 1635
PfoI T'CCnGG_A
Cuts at: 0 1890 2295
Size: 1890 405
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1698 1730 2295
Size: 1698 32 565
Fragments arranged by size:
1698 565 32
PpuMI rG'GwC_Cy
Cuts at: 0 131 2295
Size: 131 2164
PspOMI G'GGCC_C
Cuts at: 0 245 2295
Size: 245 2050
PstI C_TGCA'G
Cuts at: 0 215 470 2295
Size: 215 255 1825
Fragments arranged by size:
1825 255 215
SanDI GG'GwC_CC
Cuts at: 0 131 2295
Size: 131 2164
SapI GCTCTTCn'nnn_
Cuts at: 0 1925 2295
Size: 1925 370
ScaI AGT'ACT
Cuts at: 0 898 2295
Size: 898 1397
SfcI C'TryA_G
Cuts at: 0 211 466 2295
Size: 211 255 1829
Fragments arranged by size:
1829 255 211
SmaI CCC'GGG
Cuts at: 0 203 311 2295
Size: 203 108 1984
Fragments arranged by size:
1984 203 108
SmlI C'TyrA_G
Cuts at: 0 1118 1357 2295
Size: 1118 239 938
Fragments arranged by size:
1118 938 239
SspI AAT'ATT
Cuts at: 0 906 1687 2295
Size: 906 781 608
Fragments arranged by size:
906 781 608
StyI C'CwwG_G
Cuts at: 0 2270 2295
Size: 2270 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 157 355 793 2155 2295
Size: 157 198 438 1362 140
Fragments arranged by size:
1362 438 198 157 140
TatI w'GTAC_w
Cuts at: 0 896 2295
Size: 896 1399
Tth111I GACn'n_nGTC
Cuts at: 0 1102 2295
Size: 1102 1193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2109 2295
Size: 2109 186
XmaI C'CCGG_G
Cuts at: 0 201 309 2295
Size: 201 108 1986
Fragments arranged by size:
1986 201 108
XmnI GAAnn'nnTTC
Cuts at: 0 2141 2295
Size: 2141 154
Enzymes that do cut:
Acc65I AcuI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbvCI BciVI BglII Bme1580I BpmI Bpu10I BpuEI
BsaI BsaBI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BsrBI BsrDI BstXI BstYI BtgI BtsI DraI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII HpaI KpnI MmeI MscI MslI MspA1I
NspI PflMI PfoI PpiI PpuMI PspOMI PstI SanDI
SapI ScaI SfcI SmaI SmlI SspI StyI TaqII
TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BcgI BclI BfrBI BglI
BlpI BmgBI BmrI BmtI BplI BsaAI BsaHI BsaWI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DrdI EagI EcoICRI EcoRI EcoRV
FseI FspI FspAI HindIII KasI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI StuI SwaI TstI XbaI XhoI ZraI