Restriction map for
pF1KE1072
(Linear) (Six-base) MAPSORT of: KIEE1072.seq Check: 3370 from: 1 to: 2313
KIEE1072.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2192 2313
Size: 2192 121
AfeI AGC'GCT
Cuts at: 0 1187 2046 2313
Size: 1187 859 267
Fragments arranged by size:
1187 859 267
AflIII A'CryG_T
Cuts at: 0 845 1438 2313
Size: 845 593 875
Fragments arranged by size:
875 845 593
AhdI GACnn_n'nnGTC
Cuts at: 0 256 1039 2313
Size: 256 783 1274
Fragments arranged by size:
1274 783 256
AleI CACnn'nnGTG
Cuts at: 0 163 1831 2313
Size: 163 1668 482
Fragments arranged by size:
1668 482 163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 795 2313
Size: 795 1518
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 718 750 1444 1476 2313
Size: 718 32 694 32 837
Fragments arranged by size:
837 718 694 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 253 352 503 2313
Size: 253 99 151 1810
Fragments arranged by size:
1810 253 151 99
ApaI G_GGCC'C
Cuts at: 0 2168 2313
Size: 2168 145
ApoI r'AATT_y
Cuts at: 0 385 624 1003 2313
Size: 385 239 379 1310
Fragments arranged by size:
1310 385 379 239
AvaI C'yCGr_G
Cuts at: 0 2048 2313
Size: 2048 265
BamHI G'GATC_C
Cuts at: 0 74 1964 2313
Size: 74 1890 349
Fragments arranged by size:
1890 349 74
BanI G'GyrC_C
Cuts at: 0 930 1534 2313
Size: 930 604 779
Fragments arranged by size:
930 779 604
BanII G_rGCy'C
Cuts at: 0 1106 2168 2313
Size: 1106 1062 145
Fragments arranged by size:
1106 1062 145
BbsI GAAGACnn'nnnn_
Cuts at: 0 2180 2313
Size: 2180 133
BclI T'GATC_A
Cuts at: 0 1820 2313
Size: 1820 493
BglI GCCn_nnn'nGGC
Cuts at: 0 1652 2313
Size: 1652 661
Bme1580I G_kGCm'C
Cuts at: 0 205 1688 2168 2313
Size: 205 1483 480 145
Fragments arranged by size:
1483 480 205 145
BmgBI CAC'GTC
Cuts at: 0 848 1128 2091 2313
Size: 848 280 963 222
Fragments arranged by size:
963 848 280 222
BmrI ACTGGGnnnn_n'
Cuts at: 0 125 729 2166 2313
Size: 125 604 1437 147
Fragments arranged by size:
1437 604 147 125
BmtI G_CTAG'C
Cuts at: 0 148 2313
Size: 148 2165
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2139 2313
Size: 2139 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 442 842 2291 2313
Size: 54 388 400 1449 22
Fragments arranged by size:
1449 400 388 54 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1230 2313
Size: 1230 1083
BsaI GGTCTCn'nnnn_
Cuts at: 0 225 1208 1269 2313
Size: 225 983 61 1044
Fragments arranged by size:
1044 983 225 61
BsaAI yAC'GTr
Cuts at: 0 2075 2313
Size: 2075 238
BsaBI GATnn'nnATC
Cuts at: 0 1234 2313
Size: 1234 1079
BsaHI Gr'CG_yC
Cuts at: 0 305 2313
Size: 305 2008
BsaWI w'CCGG_w
Cuts at: 0 1967 2313
Size: 1967 346
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 317 347 718 748 1117 1147 2313
Size: 317 30 371 30 369 30 1166
Fragments arranged by size:
1166 371 369 317 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1180 1351 2128 2313
Size: 1180 171 777 185
Fragments arranged by size:
1180 777 185 171
BseYI C'CCAG_C
Cuts at: 0 1050 1425 1641 2313
Size: 1050 375 216 672
Fragments arranged by size:
1050 672 375 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 2047 2313
Size: 58 1989 266
Fragments arranged by size:
1989 266 58
BsiEI CG_ry'CG
Cuts at: 0 446 2223 2313
Size: 446 1777 90
Fragments arranged by size:
1777 446 90
BsiHKAI G_wGCw'C
Cuts at: 0 459 1343 1709 2313
Size: 459 884 366 604
Fragments arranged by size:
884 604 459 366
BsmI GAATG_Cn'
Cuts at: 0 1361 2313
Size: 1361 952
Bsp1286I G_dGCh'C
Cuts at: 0 205 459 1106 1343 1688 1709 2168 2313
Size: 205 254 647 237 345 21 459 145
Fragments arranged by size:
647 459 345 254 237 205 145 21
BspHI T'CATG_A
Cuts at: 0 683 2313
Size: 683 1630
BsrDI GCAATG_nn'
Cuts at: 0 68 275 2313
Size: 68 207 2038
Fragments arranged by size:
2038 207 68
BsrFI r'CCGG_y
Cuts at: 0 1664 2313
Size: 1664 649
BstEII G'GTnAC_C
Cuts at: 0 1692 2313
Size: 1692 621
BstXI CCAn_nnnn'nTGG
Cuts at: 0 93 243 1797 2313
Size: 93 150 1554 516
Fragments arranged by size:
1554 516 150 93
BstYI r'GATC_y
Cuts at: 0 74 349 1964 2313
Size: 74 275 1615 349
Fragments arranged by size:
1615 349 275 74
BtgI C'CryG_G
Cuts at: 0 125 744 1088 1538 2313
Size: 125 619 344 450 775
Fragments arranged by size:
775 619 450 344 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 795 2313
Size: 795 1518
BtsI GCAGTG_nn'
Cuts at: 0 46 437 866 2313
Size: 46 391 429 1447
Fragments arranged by size:
1447 429 391 46
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1422 1457 2313
Size: 1422 35 856
Fragments arranged by size:
1422 856 35
DraIII CAC_nnn'GTG
Cuts at: 0 1692 2313
Size: 1692 621
EaeI y'GGCC_r
Cuts at: 0 233 443 1013 2220 2313
Size: 233 210 570 1207 93
Fragments arranged by size:
1207 570 233 210 93
EagI C'GGCC_G
Cuts at: 0 443 2220 2313
Size: 443 1777 93
Fragments arranged by size:
1777 443 93
EarI CTCTTCn'nnn_
Cuts at: 0 703 1066 1720 2048 2236 2313
Size: 703 363 654 328 188 77
Fragments arranged by size:
703 654 363 328 188 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 526 739 2313
Size: 526 213 1574
Fragments arranged by size:
1574 526 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 442 842 2192 2291 2313
Size: 54 388 400 1350 99 22
Fragments arranged by size:
1350 400 388 99 54 22
EcoO109I rG'GnC_Cy
Cuts at: 0 250 1055 2058 2274 2313
Size: 250 805 1003 216 39
Fragments arranged by size:
1003 805 250 216 39
EcoRI G'AATT_C
Cuts at: 0 624 2313
Size: 624 1689
HaeII r_GCGC'y
Cuts at: 0 1189 2048 2313
Size: 1189 859 265
Fragments arranged by size:
1189 859 265
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 242 274 317 349 906 938
Size: 160 32 50 32 43 32 557 32
Cuts at: 938 940 972 1025 1057 1216 1248 1599 1631
Size: 2 32 53 32 159 32 351 32
Cuts at: 1631 1753 1785 2003 2035 2089 2121 2313
Size: 122 32 218 32 54 32 192
Fragments arranged by size:
557 351 218 192 160 159 122 54
53 50 43 32 32 32 32 32
32 32 32 32 32 32 2
HincII GTy'rAC
Cuts at: 0 1505 1518 2313
Size: 1505 13 795
Fragments arranged by size:
1505 795 13
HindIII A'AGCT_T
Cuts at: 0 1385 2313
Size: 1385 928
MscI TGG'CCA
Cuts at: 0 235 1015 2313
Size: 235 780 1298
Fragments arranged by size:
1298 780 235
MslI CAynn'nnrTG
Cuts at: 0 163 208 214 970 1702 1723 1831 1921
Size: 163 45 6 756 732 21 108 90
Cuts at: 1921 2313
Size: 392
Fragments arranged by size:
756 732 392 163 108 90 45 21
6
MspA1I CmG'CkG
Cuts at: 0 194 503 2313
Size: 194 309 1810
Fragments arranged by size:
1810 309 194
NcoI C'CATG_G
Cuts at: 0 1088 2313
Size: 1088 1225
NdeI CA'TA_TG
Cuts at: 0 897 2313
Size: 897 1416
NheI G'CTAG_C
Cuts at: 0 144 2313
Size: 144 2169
NspI r_CATG'y
Cuts at: 0 213 471 782 1442 2313
Size: 213 258 311 660 871
Fragments arranged by size:
871 660 311 258 213
PciI A'CATG_T
Cuts at: 0 1438 2313
Size: 1438 875
PflMI CCAn_nnn'nTGG
Cuts at: 0 1961 2313
Size: 1961 352
PfoI T'CCnGG_A
Cuts at: 0 70 419 2313
Size: 70 349 1894
Fragments arranged by size:
1894 349 70
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 718 750 2313
Size: 718 32 1563
Fragments arranged by size:
1563 718 32
PpuMI rG'GwC_Cy
Cuts at: 0 250 1055 2313
Size: 250 805 1258
Fragments arranged by size:
1258 805 250
PspOMI G'GGCC_C
Cuts at: 0 2164 2313
Size: 2164 149
PstI C_TGCA'G
Cuts at: 0 1850 2313
Size: 1850 463
PvuII CAG'CTG
Cuts at: 0 194 2313
Size: 194 2119
SanDI GG'GwC_CC
Cuts at: 0 1055 2313
Size: 1055 1258
SapI GCTCTTCn'nnn_
Cuts at: 0 1720 2236 2313
Size: 1720 516 77
Fragments arranged by size:
1720 516 77
SexAI A'CCwGG_T
Cuts at: 0 252 2313
Size: 252 2061
SfcI C'TryA_G
Cuts at: 0 363 1846 2313
Size: 363 1483 467
Fragments arranged by size:
1483 467 363
SmlI C'TyrA_G
Cuts at: 0 1209 2048 2313
Size: 1209 839 265
Fragments arranged by size:
1209 839 265
SspI AAT'ATT
Cuts at: 0 1581 2313
Size: 1581 732
StuI AGG'CCT
Cuts at: 0 695 2313
Size: 695 1618
StyI C'CwwG_G
Cuts at: 0 236 1088 1259 1463 2313
Size: 236 852 171 204 850
Fragments arranged by size:
852 850 236 204 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 663 2313
Size: 663 1650
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1117 1149 2313
Size: 1117 32 1164
Fragments arranged by size:
1164 1117 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1008 2313
Size: 1008 1305
XhoI C'TCGA_G
Cuts at: 0 2048 2313
Size: 2048 265
Enzymes that do cut:
AcuI AfeI AflIII AhdI AleI AlfI AloI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbsI BclI
BglI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraIII
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI HaeII
Hin4I HincII HindIII MscI MslI MspA1I NcoI NdeI
NheI NspI PciI PflMI PfoI PpiI PpuMI PspOMI
PstI PvuII SanDI SapI SexAI SfcI SmlI SspI
StuI StyI TaqII TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI
BciVI BfrBI BglII BlpI Bpu10I BsiWI BsmBI BspEI
BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I ClaI DraI DrdI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NgoMIV NotI NruI NsiI PacI
PasI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TatI Tth111I XbaI XmaI XmnI ZraI