Restriction map for
pF1KE1074
(Linear) (Six-base) MAPSORT of: KIEE1074.seq Check: 3706 from: 1 to: 2130
KIEE1074.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 0 1406 2130
Size: 1406 724
AfeI AGC'GCT
Cuts at: 0 601 2130
Size: 601 1529
AflII C'TTAA_G
Cuts at: 0 973 2130
Size: 973 1157
AflIII A'CryG_T
Cuts at: 0 1040 2130
Size: 1040 1090
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2003 2130
Size: 2003 127
AlwNI CAG_nnn'CTG
Cuts at: 0 1557 2130
Size: 1557 573
ApaLI G'TGCA_C
Cuts at: 0 1786 2130
Size: 1786 344
BamHI G'GATC_C
Cuts at: 0 1338 2130
Size: 1338 792
BanII G_rGCy'C
Cuts at: 0 67 2130
Size: 67 2063
BbsI GAAGACnn'nnnn_
Cuts at: 0 697 912 2074 2130
Size: 697 215 1162 56
Fragments arranged by size:
1162 697 215 56
BbvCI CC'TCA_GC
Cuts at: 0 763 2130
Size: 763 1367
BciVI GTATCCnnnnn_n'
Cuts at: 0 1623 1863 2130
Size: 1623 240 267
Fragments arranged by size:
1623 267 240
BclI T'GATC_A
Cuts at: 0 891 1145 2130
Size: 891 254 985
Fragments arranged by size:
985 891 254
BfrBI ATG'CAT
Cuts at: 0 241 514 2130
Size: 241 273 1616
Fragments arranged by size:
1616 273 241
BglII A'GATC_T
Cuts at: 0 1921 2130
Size: 1921 209
BlpI GC'TnA_GC
Cuts at: 0 1744 2130
Size: 1744 386
Bme1580I G_kGCm'C
Cuts at: 0 1790 2130
Size: 1790 340
BmrI ACTGGGnnnn_n'
Cuts at: 0 2031 2130
Size: 2031 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1159 2130
Size: 1006 153 971
Fragments arranged by size:
1006 971 153
Bpu10I CC'TnA_GC
Cuts at: 0 763 2130
Size: 763 1367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1020 2130
Size: 1020 1110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 2130
Size: 772 1358
BsiHKAI G_wGCw'C
Cuts at: 0 1455 1790 2130
Size: 1455 335 340
Fragments arranged by size:
1455 340 335
BsmI GAATG_Cn'
Cuts at: 0 206 241 1099 1611 1992 2130
Size: 206 35 858 512 381 138
Fragments arranged by size:
858 512 381 206 138 35
Bsp1286I G_dGCh'C
Cuts at: 0 67 1455 1790 2130
Size: 67 1388 335 340
Fragments arranged by size:
1388 340 335 67
BspHI T'CATG_A
Cuts at: 0 408 2130
Size: 408 1722
BsrDI GCAATG_nn'
Cuts at: 0 566 842 1997 2130
Size: 566 276 1155 133
Fragments arranged by size:
1155 566 276 133
BstAPI GCAn_nnn'nTGC
Cuts at: 0 759 967 2130
Size: 759 208 1163
Fragments arranged by size:
1163 759 208
BstEII G'GTnAC_C
Cuts at: 0 768 2130
Size: 768 1362
BstYI r'GATC_y
Cuts at: 0 1191 1338 1921 2130
Size: 1191 147 583 209
Fragments arranged by size:
1191 583 209 147
BtgI C'CryG_G
Cuts at: 0 1122 1696 2130
Size: 1122 574 434
Fragments arranged by size:
1122 574 434
DraI TTT'AAA
Cuts at: 0 182 252 2130
Size: 182 70 1878
Fragments arranged by size:
1878 182 70
EarI CTCTTCn'nnn_
Cuts at: 0 1201 1318 1395 2099 2130
Size: 1201 117 77 704 31
Fragments arranged by size:
1201 704 117 77 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1159 2130
Size: 1006 153 971
Fragments arranged by size:
1006 971 153
EcoRV GAT'ATC
Cuts at: 0 216 226 2130
Size: 216 10 1904
Fragments arranged by size:
1904 216 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 418 448 2130
Size: 418 30 1682
Fragments arranged by size:
1682 418 30
HaeII r_GCGC'y
Cuts at: 0 529 603 2130
Size: 529 74 1527
Fragments arranged by size:
1527 529 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1330 1362 1449 1481 1693 1725 2130
Size: 1330 32 87 32 212 32 405
Fragments arranged by size:
1330 405 212 87 32 32 32
HincII GTy'rAC
Cuts at: 0 5 821 2130
Size: 5 816 1309
Fragments arranged by size:
1309 816 5
HindIII A'AGCT_T
Cuts at: 0 721 2130
Size: 721 1409
MfeI C'AATT_G
Cuts at: 0 569 2130
Size: 569 1561
MslI CAynn'nnrTG
Cuts at: 0 559 1721 1985 2130
Size: 559 1162 264 145
Fragments arranged by size:
1162 559 264 145
MspA1I CmG'CkG
Cuts at: 0 1692 1698 2130
Size: 1692 6 432
Fragments arranged by size:
1692 432 6
NcoI C'CATG_G
Cuts at: 0 1122 2130
Size: 1122 1008
NdeI CA'TA_TG
Cuts at: 0 656 2130
Size: 656 1474
NsiI A_TGCA'T
Cuts at: 0 243 516 2130
Size: 243 273 1614
Fragments arranged by size:
1614 273 243
NspI r_CATG'y
Cuts at: 0 1044 2130
Size: 1044 1086
PciI A'CATG_T
Cuts at: 0 1040 2130
Size: 1040 1090
PflMI CCAn_nnn'nTGG
Cuts at: 0 1188 2130
Size: 1188 942
PfoI T'CCnGG_A
Cuts at: 0 1085 2130
Size: 1085 1045
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 682 714 2130
Size: 682 32 1416
Fragments arranged by size:
1416 682 32
PvuII CAG'CTG
Cuts at: 0 1692 2130
Size: 1692 438
SacII CC_GC'GG
Cuts at: 0 1699 2130
Size: 1699 431
SapI GCTCTTCn'nnn_
Cuts at: 0 2099 2130
Size: 2099 31
ScaI AGT'ACT
Cuts at: 0 1052 2130
Size: 1052 1078
SfcI C'TryA_G
Cuts at: 0 1883 2130
Size: 1883 247
SmlI C'TyrA_G
Cuts at: 0 973 999 2130
Size: 973 26 1131
Fragments arranged by size:
1131 973 26
SpeI A'CTAG_T
Cuts at: 0 885 2130
Size: 885 1245
StyI C'CwwG_G
Cuts at: 0 437 1122 1284 2130
Size: 437 685 162 846
Fragments arranged by size:
846 685 437 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1115 2130
Size: 1115 1015
TatI w'GTAC_w
Cuts at: 0 94 1050 2130
Size: 94 956 1080
Fragments arranged by size:
1080 956 94
XmnI GAAnn'nnTTC
Cuts at: 0 30 428 2093 2130
Size: 30 398 1665 37
Fragments arranged by size:
1665 398 37 30
Enzymes that do cut:
AccI AfeI AflII AflIII AlfI AlwNI ApaLI BamHI
BanII BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BstAPI BstEII BstYI BtgI DraI
EarI Eco57MI EcoRV FalI HaeII Hin4I HincII HindIII
MfeI MslI MspA1I NcoI NdeI NsiI NspI PciI
PflMI PfoI PsrI PvuII SacII SapI ScaI SfcI
SmlI SpeI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AgeI AhdI AleI
AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BcgI BglI BmgBI BmtI BplI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI FseI FspI FspAI HpaI KasI
KpnI MluI MmeI MscI NaeI NarI NgoMIV NheI
NotI NruI PacI PasI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI