Restriction map for
pF1KE1080
(Linear) (Six-base) MAPSORT of: KIEE1080.seq Check: 7873 from: 1 to: 3774
KIEE1080.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 742 3774
Size: 742 3032
AccI GT'mk_AC
Cuts at: 0 1853 3774
Size: 1853 1921
AclI AA'CG_TT
Cuts at: 0 3642 3774
Size: 3642 132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 691 1256 2330 3317 3689 3774
Size: 54 637 565 1074 987 372 85
Fragments arranged by size:
1074 987 637 565 372 85 54
AflII C'TTAA_G
Cuts at: 0 3630 3774
Size: 3630 144
AflIII A'CryG_T
Cuts at: 0 1573 1963 2370 3048 3774
Size: 1573 390 407 678 726
Fragments arranged by size:
1573 726 678 407 390
AleI CACnn'nnGTG
Cuts at: 0 1781 3774
Size: 1781 1993
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 961 993 3774
Size: 961 32 2781
Fragments arranged by size:
2781 961 32
AlwNI CAG_nnn'CTG
Cuts at: 0 743 2986 3562 3774
Size: 743 2243 576 212
Fragments arranged by size:
2243 743 576 212
ApaLI G'TGCA_C
Cuts at: 0 3525 3774
Size: 3525 249
ApoI r'AATT_y
Cuts at: 0 343 362 913 1047 1116 1324 2439 2473
Size: 343 19 551 134 69 208 1115 34
Cuts at: 2473 3031 3180 3332 3467 3774
Size: 558 149 152 135 307
Fragments arranged by size:
1115 558 551 343 307 208 152 149
135 134 69 34 19
AseI AT'TA_AT
Cuts at: 0 1246 3774
Size: 1246 2528
AvaI C'yCGr_G
Cuts at: 0 1376 3774
Size: 1376 2398
AvrII C'CTAG_G
Cuts at: 0 544 2209 3774
Size: 544 1665 1565
Fragments arranged by size:
1665 1565 544
BanI G'GyrC_C
Cuts at: 0 1206 3774
Size: 1206 2568
BanII G_rGCy'C
Cuts at: 0 2385 3774
Size: 2385 1389
BbsI GAAGACnn'nnnn_
Cuts at: 0 565 2108 2354 3774
Size: 565 1543 246 1420
Fragments arranged by size:
1543 1420 565 246
BbvCI CC'TCA_GC
Cuts at: 0 659 3747 3774
Size: 659 3088 27
Fragments arranged by size:
3088 659 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 601 621 635 655 3774
Size: 601 20 14 20 3119
Fragments arranged by size:
3119 601 20 20 14
BciVI GTATCCnnnnn_n'
Cuts at: 0 1819 3774
Size: 1819 1955
BclI T'GATC_A
Cuts at: 0 1926 2931 3774
Size: 1926 1005 843
Fragments arranged by size:
1926 1005 843
BfrBI ATG'CAT
Cuts at: 0 232 2776 2803 3774
Size: 232 2544 27 971
Fragments arranged by size:
2544 971 232 27
Bme1580I G_kGCm'C
Cuts at: 0 3529 3671 3774
Size: 3529 142 103
Fragments arranged by size:
3529 142 103
BmgBI CAC'GTC
Cuts at: 0 494 2352 3774
Size: 494 1858 1422
Fragments arranged by size:
1858 1422 494
BmrI ACTGGGnnnn_n'
Cuts at: 0 2291 3774
Size: 2291 1483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 2815 3774
Size: 357 2458 959
Fragments arranged by size:
2458 959 357
Bpu10I CC'TnA_GC
Cuts at: 0 659 3747 3774
Size: 659 3088 27
Fragments arranged by size:
3088 659 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2398 3774
Size: 2398 1376
BsaAI yAC'GTr
Cuts at: 0 1966 3774
Size: 1966 1808
BsaBI GATnn'nnATC
Cuts at: 0 3140 3774
Size: 3140 634
BsaHI Gr'CG_yC
Cuts at: 0 2300 3774
Size: 2300 1474
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1931 1961 3057 3087 3774
Size: 1931 30 1096 30 687
Fragments arranged by size:
1931 1096 687 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 676 2185 2188 2206 2209 3390 3774
Size: 676 1509 3 18 3 1181 384
Fragments arranged by size:
1509 1181 676 384 18 3 3
BseYI C'CCAG_C
Cuts at: 0 3761 3774
Size: 3761 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 3774
Size: 605 3169
BsiHKAI G_wGCw'C
Cuts at: 0 1987 2385 3529 3774
Size: 1987 398 1144 245
Fragments arranged by size:
1987 1144 398 245
BsmI GAATG_Cn'
Cuts at: 0 1569 3688 3774
Size: 1569 2119 86
Fragments arranged by size:
2119 1569 86
Bsp1286I G_dGCh'C
Cuts at: 0 1987 2385 3529 3671 3774
Size: 1987 398 1144 142 103
Fragments arranged by size:
1987 1144 398 142 103
BspHI T'CATG_A
Cuts at: 0 3356 3774
Size: 3356 418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 742 3774
Size: 742 3032
BsrDI GCAATG_nn'
Cuts at: 0 764 887 1406 3774
Size: 764 123 519 2368
Fragments arranged by size:
2368 764 519 123
BsrGI T'GTAC_A
Cuts at: 0 1620 3774
Size: 1620 2154
BstEII G'GTnAC_C
Cuts at: 0 79 3774
Size: 79 3695
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1669 3774
Size: 1669 2105
BstYI r'GATC_y
Cuts at: 0 1419 3126 3581 3774
Size: 1419 1707 455 193
Fragments arranged by size:
1707 1419 455 193
BstZ17I GTA'TAC
Cuts at: 0 1854 3774
Size: 1854 1920
Bsu36I CC'TnA_GG
Cuts at: 0 3586 3774
Size: 3586 188
BtgI C'CryG_G
Cuts at: 0 126 553 3774
Size: 126 427 3221
Fragments arranged by size:
3221 427 126
ClaI AT'CG_AT
Cuts at: 0 611 3774
Size: 611 3163
DraI TTT'AAA
Cuts at: 0 237 3042 3696 3774
Size: 237 2805 654 78
Fragments arranged by size:
2805 654 237 78
EarI CTCTTCn'nnn_
Cuts at: 0 667 827 1196 1526 2162 2183 2306 2879
Size: 667 160 369 330 636 21 123 573
Cuts at: 2879 3207 3604 3654 3708 3748 3774
Size: 328 397 50 54 40 26
Fragments arranged by size:
667 636 573 397 369 330 328 160
123 54 50 40 26 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2691 3774
Size: 2691 1083
EcoICRI GAG'CTC
Cuts at: 0 2383 3774
Size: 2383 1391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 357 691 1256 2330 2815 3317 3689
Size: 54 303 334 565 1074 485 502 372
Cuts at: 3689 3774
Size: 85
Fragments arranged by size:
1074 565 502 485 372 334 303 85
54
EcoO109I rG'GnC_Cy
Cuts at: 0 147 601 1666 3758 3774
Size: 147 454 1065 2092 16
Fragments arranged by size:
2092 1065 454 147 16
EcoRI G'AATT_C
Cuts at: 0 343 362 2473 3774
Size: 343 19 2111 1301
Fragments arranged by size:
2111 1301 343 19
EcoRV GAT'ATC
Cuts at: 0 2646 3774
Size: 2646 1128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1411 1443 1810 1842 1911 1943 2290 2322
Size: 1411 32 367 32 69 32 347 32
Cuts at: 2322 2616 2648 3057 3089 3241 3273 3774
Size: 294 32 409 32 152 32 501
Fragments arranged by size:
1411 501 409 367 347 294 152 69
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 256 1465 1824 1919 3293 3500 3774
Size: 256 1209 359 95 1374 207 274
Fragments arranged by size:
1374 1209 359 274 256 207 95
HpaI GTT'AAC
Cuts at: 0 1824 3293 3774
Size: 1824 1469 481
Fragments arranged by size:
1824 1469 481
MfeI C'AATT_G
Cuts at: 0 2603 3774
Size: 2603 1171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1005 1875 3774
Size: 1005 870 1899
Fragments arranged by size:
1899 1005 870
MslI CAynn'nnrTG
Cuts at: 0 1616 1781 3774
Size: 1616 165 1993
Fragments arranged by size:
1993 1616 165
MspA1I CmG'CkG
Cuts at: 0 1687 3559 3774
Size: 1687 1872 215
Fragments arranged by size:
1872 1687 215
NcoI C'CATG_G
Cuts at: 0 126 3774
Size: 126 3648
NdeI CA'TA_TG
Cuts at: 0 228 3774
Size: 228 3546
NsiI A_TGCA'T
Cuts at: 0 234 2778 2805 3774
Size: 234 2544 27 969
Fragments arranged by size:
2544 969 234 27
NspI r_CATG'y
Cuts at: 0 1577 1948 2374 3052 3774
Size: 1577 371 426 678 722
Fragments arranged by size:
1577 722 678 426 371
PciI A'CATG_T
Cuts at: 0 1573 2370 3048 3774
Size: 1573 797 678 726
Fragments arranged by size:
1573 797 726 678
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2198 2230 3774
Size: 2198 32 1544
Fragments arranged by size:
2198 1544 32
PpuMI rG'GwC_Cy
Cuts at: 0 1666 3758 3774
Size: 1666 2092 16
Fragments arranged by size:
2092 1666 16
PshAI GACnn'nnGTC
Cuts at: 0 1239 3774
Size: 1239 2535
PsiI TTA'TAA
Cuts at: 0 373 3187 3774
Size: 373 2814 587
Fragments arranged by size:
2814 587 373
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3409 3441 3774
Size: 3409 32 333
Fragments arranged by size:
3409 333 32
PstI C_TGCA'G
Cuts at: 0 445 739 1179 3774
Size: 445 294 440 2595
Fragments arranged by size:
2595 445 440 294
PvuII CAG'CTG
Cuts at: 0 1687 3559 3774
Size: 1687 1872 215
Fragments arranged by size:
1872 1687 215
SacI G_AGCT'C
Cuts at: 0 2385 3774
Size: 2385 1389
SapI GCTCTTCn'nnn_
Cuts at: 0 1526 3708 3774
Size: 1526 2182 66
Fragments arranged by size:
2182 1526 66
SfcI C'TryA_G
Cuts at: 0 249 441 719 735 1026 1175 3774
Size: 249 192 278 16 291 149 2599
Fragments arranged by size:
2599 291 278 249 192 149 16
SmlI C'TyrA_G
Cuts at: 0 2413 3630 3774
Size: 2413 1217 144
Fragments arranged by size:
2413 1217 144
SspI AAT'ATT
Cuts at: 0 1250 2900 2919 3774
Size: 1250 1650 19 855
Fragments arranged by size:
1650 1250 855 19
StyI C'CwwG_G
Cuts at: 0 126 544 1662 2209 3774
Size: 126 418 1118 547 1565
Fragments arranged by size:
1565 1118 547 418 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1284 3774
Size: 1284 2490
TatI w'GTAC_w
Cuts at: 0 728 1620 2077 2130 3479 3774
Size: 728 892 457 53 1349 295
Fragments arranged by size:
1349 892 728 457 295 53
Tth111I GACn'n_nGTC
Cuts at: 0 2515 3774
Size: 2515 1259
XbaI T'CTAG_A
Cuts at: 0 2779 3774
Size: 2779 995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 498 900 2626 3602 3774
Size: 498 402 1726 976 172
Fragments arranged by size:
1726 976 498 402 172
XmnI GAAnn'nnTTC
Cuts at: 0 2153 2556 2614 3774
Size: 2153 403 58 1160
Fragments arranged by size:
2153 1160 403 58
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AleI AloI
AlwNI ApaLI ApoI AseI AvaI AvrII BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrGI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
ClaI DraI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV Hin4I HincII HpaI MfeI MmeI MslI MspA1I
NcoI NdeI NsiI NspI PciI PpiI PpuMI PshAI
PsiI PsrI PstI PvuII SacI SapI SfcI SmlI
SspI StyI TaqII TatI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AgeI AhdI AlfI ApaI AscI
AsiSI BaeI BamHI BbeI BglI BglII BlpI BmtI
BplI BsaI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BtgZI BtsI CspCI
DraIII DrdI EaeI EagI EcoNI FalI FseI FspI
FspAI HaeII HindIII KasI KpnI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PflMI
PfoI PmeI PmlI PspOMI PspXI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TstI XhoI XmaI ZraI