Restriction map for
pF1KE1082
(Linear) (Six-base) MAPSORT of: KIEE1082.seq Check: 2390 from: 1 to: 1317
KIEE1082.orf
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AclI AA'CG_TT
Cuts at: 0 11 1317
Size: 11 1306
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1265 1317
Size: 1265 52
AlwNI CAG_nnn'CTG
Cuts at: 0 509 540 835 1317
Size: 509 31 295 482
Fragments arranged by size:
509 482 295 31
ApoI r'AATT_y
Cuts at: 0 154 1317
Size: 154 1163
AvaI C'yCGr_G
Cuts at: 0 703 1317
Size: 703 614
BanI G'GyrC_C
Cuts at: 0 129 896 1317
Size: 129 767 421
Fragments arranged by size:
767 421 129
BanII G_rGCy'C
Cuts at: 0 206 710 1317
Size: 206 504 607
Fragments arranged by size:
607 504 206
BbeI G_GCGC'C
Cuts at: 0 133 1317
Size: 133 1184
BbsI GAAGACnn'nnnn_
Cuts at: 0 573 1317
Size: 573 744
BmgBI CAC'GTC
Cuts at: 0 903 1317
Size: 903 414
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 830 1317
Size: 830 487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 504 621 872 1317
Size: 316 188 117 251 445
Fragments arranged by size:
445 316 251 188 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1317
Size: 874 443
BsaI GGTCTCn'nnnn_
Cuts at: 0 358 1317
Size: 358 959
BsaHI Gr'CG_yC
Cuts at: 0 130 1317
Size: 130 1187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 314 344 1317
Size: 314 30 973
Fragments arranged by size:
973 314 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 97 335 663 739 772 860 1066
Size: 94 3 238 328 76 33 88 206
Cuts at: 1066 1110 1240 1317
Size: 44 130 77
Fragments arranged by size:
328 238 206 130 94 88 77 76
44 33 3
BseYI C'CCAG_C
Cuts at: 0 133 315 1317
Size: 133 182 1002
Fragments arranged by size:
1002 182 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1317
Size: 76 1241
BsiEI CG_ry'CG
Cuts at: 0 287 419 1317
Size: 287 132 898
Fragments arranged by size:
898 287 132
BsiHKAI G_wGCw'C
Cuts at: 0 719 1317
Size: 719 598
BsmBI CGTCTCn'nnnn_
Cuts at: 0 438 909 1317
Size: 438 471 408
Fragments arranged by size:
471 438 408
Bsp1286I G_dGCh'C
Cuts at: 0 206 710 719 1317
Size: 206 504 9 598
Fragments arranged by size:
598 504 206 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 539 1317
Size: 539 778
BsrBI CCG'CTC
Cuts at: 0 197 407 1317
Size: 197 210 910
Fragments arranged by size:
910 210 197
BssHII G'CGCG_C
Cuts at: 0 463 1317
Size: 463 854
BstEII G'GTnAC_C
Cuts at: 0 303 1317
Size: 303 1014
BstYI r'GATC_y
Cuts at: 0 537 1139 1317
Size: 537 602 178
Fragments arranged by size:
602 537 178
BtgI C'CryG_G
Cuts at: 0 281 495 1317
Size: 281 214 822
Fragments arranged by size:
822 281 214
ClaI AT'CG_AT
Cuts at: 0 785 1317
Size: 785 532
EaeI y'GGCC_r
Cuts at: 0 284 416 498 1317
Size: 284 132 82 819
Fragments arranged by size:
819 284 132 82
EagI C'GGCC_G
Cuts at: 0 284 416 1317
Size: 284 132 901
Fragments arranged by size:
901 284 132
EarI CTCTTCn'nnn_
Cuts at: 0 1241 1317
Size: 1241 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 504 621 872 1265 1317
Size: 316 188 117 251 393 52
Fragments arranged by size:
393 316 251 188 117 52
EcoO109I rG'GnC_Cy
Cuts at: 0 1037 1167 1317
Size: 1037 130 150
Fragments arranged by size:
1037 150 130
EcoRV GAT'ATC
Cuts at: 0 144 1317
Size: 144 1173
HaeII r_GCGC'y
Cuts at: 0 133 548 644 934 1092 1317
Size: 133 415 96 290 158 225
Fragments arranged by size:
415 290 225 158 133 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 592 624 823 855 1317
Size: 34 32 526 32 199 32 462
Fragments arranged by size:
526 462 199 34 32 32 32
KasI G'GCGC_C
Cuts at: 0 129 1317
Size: 129 1188
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 1130 1317
Size: 959 171 187
Fragments arranged by size:
959 187 171
MspA1I CmG'CkG
Cuts at: 0 294 476 497 1317
Size: 294 182 21 820
Fragments arranged by size:
820 294 182 21
NarI GG'CG_CC
Cuts at: 0 130 1317
Size: 130 1187
PfoI T'CCnGG_A
Cuts at: 0 389 1142 1317
Size: 389 753 175
Fragments arranged by size:
753 389 175
PpuMI rG'GwC_Cy
Cuts at: 0 1037 1317
Size: 1037 280
PstI C_TGCA'G
Cuts at: 0 122 536 1317
Size: 122 414 781
Fragments arranged by size:
781 414 122
PvuII CAG'CTG
Cuts at: 0 294 1317
Size: 294 1023
SacII CC_GC'GG
Cuts at: 0 498 1317
Size: 498 819
SbfI CC_TGCA'GG
Cuts at: 0 536 1317
Size: 536 781
SfcI C'TryA_G
Cuts at: 0 118 532 1317
Size: 118 414 785
Fragments arranged by size:
785 414 118
SfoI GGC'GCC
Cuts at: 0 131 1317
Size: 131 1186
SmlI C'TyrA_G
Cuts at: 0 889 1317
Size: 889 428
StyI C'CwwG_G
Cuts at: 0 1172 1317
Size: 1172 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 322 1317
Size: 322 995
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 314 346 385 417 1296 1317
Size: 314 32 39 32 879 21
Fragments arranged by size:
879 314 39 32 32 21
Tth111I GACn'n_nGTC
Cuts at: 0 681 985 1317
Size: 681 304 332
Fragments arranged by size:
681 332 304
XmnI GAAnn'nnTTC
Cuts at: 0 154 1317
Size: 154 1163
Enzymes that do cut:
AclI AcuI AlwNI ApoI AvaI BanI BanII BbeI
BbsI BmgBI BplI BpmI BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BssHII BstEII BstYI BtgI ClaI EaeI EagI
EarI Eco57MI EcoO109I EcoRV HaeII Hin4I KasI MmeI
MspA1I NarI PfoI PpuMI PstI PvuII SacII SbfI
SfcI SfoI SmlI StyI TaqII TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI Bpu10I
BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI BtsI CspCI DraI DraIII DrdI EciI EcoICRI
EcoNI EcoRI FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI XbaI
XcmI XhoI XmaI ZraI