(Linear) (Six-base) MAPSORT of: KIEE1083.seq  Check: 295  from: 1  to: 1110



KIEE1083.orf

 With 182 enzymes: SgfI * 

                        July 22, 2009 17:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     815    1030    1110 
   Size:        815     215      80

  Fragments arranged by size: 

                 815     215      80
 
ApoI r'AATT_y

Cuts at:      0     399    1110 
   Size:        399     711
 
AseI AT'TA_AT

Cuts at:      0     424    1110 
   Size:        424     686
 
BanII G_rGCy'C

Cuts at:      0     696    1110 
   Size:        696     414
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1043    1110 
   Size:       1043      67
 
BfrBI ATG'CAT

Cuts at:      0     558    1110 
   Size:        558     552
 
BglII A'GATC_T

Cuts at:      0     741    1110 
   Size:        741     369
 
Bpu10I CC'TnA_GC

Cuts at:      0     304     654    1110 
   Size:        304     350     456

  Fragments arranged by size: 

                 456     350     304
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     779    1110 
   Size:        779     331
 
BsaBI GATnn'nnATC

Cuts at:      0     437    1110 
   Size:        437     673
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     139     142     167     691    1110 
   Size:        139       3      25     524     419

  Fragments arranged by size: 

                 524     419     139      25       3
 
BsiHKAI G_wGCw'C

Cuts at:      0      45    1002    1110 
   Size:         45     957     108

  Fragments arranged by size: 

                 957     108      45
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     843    1110 
   Size:        843     267
 
Bsp1286I G_dGCh'C

Cuts at:      0      45     696    1002    1110 
   Size:         45     651     306     108

  Fragments arranged by size: 

                 651     306     108      45
 
BsrDI GCAATG_nn'

Cuts at:      0      48     249    1110 
   Size:         48     201     861

  Fragments arranged by size: 

                 861     201      48
 
BssSI C'ACGA_G

Cuts at:      0      16    1110 
   Size:         16    1094
 
BstYI r'GATC_y

Cuts at:      0     741    1110 
   Size:        741     369
 
BtgI C'CryG_G

Cuts at:      0      89    1110 
   Size:         89    1021
 
ClaI AT'CG_AT

Cuts at:      0     311    1110 
   Size:        311     799
 
DraIII CAC_nnn'GTG

Cuts at:      0      40    1110 
   Size:         40    1070
 
EarI CTCTTCn'nnn_

Cuts at:      0     830     841    1110 
   Size:        830      11     269

  Fragments arranged by size: 

                 830     269      11
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     815    1030    1110 
   Size:        815     215      80

  Fragments arranged by size: 

                 815     215      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0     945    1110 
   Size:        945     165
 
EcoRI G'AATT_C

Cuts at:      0     399    1110 
   Size:        399     711
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      52    1060    1110 
   Size:         52    1008      50

  Fragments arranged by size: 

                1008      52      50
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     951     983    1110 
   Size:        951      32     127

  Fragments arranged by size: 

                 951     127      32
 
MfeI C'AATT_G

Cuts at:      0     104    1110 
   Size:        104    1006
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     573    1110 
   Size:        573     537
 
MslI CAynn'nnrTG

Cuts at:      0     336     969     971    1110 
   Size:        336     633       2     139

  Fragments arranged by size: 

                 633     336     139       2
 
MspA1I CmG'CkG

Cuts at:      0     117    1054    1110 
   Size:        117     937      56

  Fragments arranged by size: 

                 937     117      56
 
NsiI A_TGCA'T

Cuts at:      0     560    1110 
   Size:        560     550
 
NspI r_CATG'y

Cuts at:      0    1044    1110 
   Size:       1044      66
 
PpuMI rG'GwC_Cy

Cuts at:      0     945    1110 
   Size:        945     165
 
PsiI TTA'TAA

Cuts at:      0     238     347    1110 
   Size:        238     109     763

  Fragments arranged by size: 

                 763     238     109
 
PstI C_TGCA'G

Cuts at:      0     536    1110 
   Size:        536     574
 
PvuII CAG'CTG

Cuts at:      0     117    1054    1110 
   Size:        117     937      56

  Fragments arranged by size: 

                 937     117      56
 
SapI GCTCTTCn'nnn_

Cuts at:      0     830    1110 
   Size:        830     280
 
SfcI C'TryA_G

Cuts at:      0     532    1110 
   Size:        532     578
 
SmlI C'TyrA_G

Cuts at:      0     794    1110 
   Size:        794     316
 
SpeI A'CTAG_T

Cuts at:      0     160    1110 
   Size:        160     950
 
SspI AAT'ATT

Cuts at:      0      79    1110 
   Size:         79    1031
 
Tth111I GACn'n_nGTC

Cuts at:      0    1062    1110 
   Size:       1062      48
 
XmnI GAAnn'nnTTC

Cuts at:      0     839    1110 
   Size:        839     271
 

 Enzymes that do cut:

     AcuI     ApoI     AseI    BanII     BbsI    BfrBI    BglII   Bpu10I
    BpuEI    BsaBI    BseRI  BsiHKAI    BsmBI Bsp1286I    BsrDI    BssSI
    BstYI     BtgI     ClaI   DraIII     EarI  Eco57MI EcoO109I    EcoRI
     FalI    Hin4I     MfeI     MmeI     MslI   MspA1I     NsiI     NspI
    PpuMI     PsiI     PstI    PvuII     SapI     SfcI     SmlI     SpeI
     SspI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI    CspCI     DraI     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TatI     TstI     XbaI     XcmI     XhoI     XmaI
     ZraI