Restriction map for
pF1KE1090
(Linear) (Six-base) MAPSORT of: KIEE1090.seq Check: 2706 from: 1 to: 1785
KIEE1090.orf
With 182 enzymes: SgfI *
October 13, 2009 15:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 861 1785
Size: 861 924
AflIII A'CryG_T
Cuts at: 0 851 1560 1785
Size: 851 709 225
Fragments arranged by size:
851 709 225
AleI CACnn'nnGTG
Cuts at: 0 190 850 1785
Size: 190 660 935
Fragments arranged by size:
935 660 190
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 369 401 1785
Size: 369 32 1384
Fragments arranged by size:
1384 369 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1082 1715 1785
Size: 1082 633 70
Fragments arranged by size:
1082 633 70
ApaLI G'TGCA_C
Cuts at: 0 181 250 1785
Size: 181 69 1535
Fragments arranged by size:
1535 181 69
ApoI r'AATT_y
Cuts at: 0 437 979 1785
Size: 437 542 806
Fragments arranged by size:
806 542 437
AvaI C'yCGr_G
Cuts at: 0 358 1785
Size: 358 1427
BamHI G'GATC_C
Cuts at: 0 59 941 1727 1785
Size: 59 882 786 58
Fragments arranged by size:
882 786 59 58
BanI G'GyrC_C
Cuts at: 0 79 500 967 1427 1475 1785
Size: 79 421 467 460 48 310
Fragments arranged by size:
467 460 421 310 79 48
BanII G_rGCy'C
Cuts at: 0 1595 1785
Size: 1595 190
BbsI GAAGACnn'nnnn_
Cuts at: 0 484 927 1785
Size: 484 443 858
Fragments arranged by size:
858 484 443
BciVI GTATCCnnnnn_n'
Cuts at: 0 374 1785
Size: 374 1411
BclI T'GATC_A
Cuts at: 0 104 1785
Size: 104 1681
BglII A'GATC_T
Cuts at: 0 764 1785
Size: 764 1021
Bme1580I G_kGCm'C
Cuts at: 0 185 254 505 1785
Size: 185 69 251 1280
Fragments arranged by size:
1280 251 185 69
BmgBI CAC'GTC
Cuts at: 0 1563 1785
Size: 1563 222
BmrI ACTGGGnnnn_n'
Cuts at: 0 780 1785
Size: 780 1005
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1059 1501 1743 1785
Size: 1059 442 242 42
Fragments arranged by size:
1059 442 242 42
BsaAI yAC'GTr
Cuts at: 0 102 1785
Size: 102 1683
BsaWI w'CCGG_w
Cuts at: 0 445 970 1730 1785
Size: 445 525 760 55
Fragments arranged by size:
760 525 445 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1057 1087 1474 1504 1785
Size: 1057 30 387 30 281
Fragments arranged by size:
1057 387 281 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 1384 1498 1785
Size: 232 1152 114 287
Fragments arranged by size:
1152 287 232 114
BseYI C'CCAG_C
Cuts at: 0 1708 1785
Size: 1708 77
BsiHKAI G_wGCw'C
Cuts at: 0 185 254 572 1785
Size: 185 69 318 1213
Fragments arranged by size:
1213 318 185 69
BsmBI CGTCTCn'nnnn_
Cuts at: 0 34 1785
Size: 34 1751
Bsp1286I G_dGCh'C
Cuts at: 0 185 254 505 572 1595 1785
Size: 185 69 251 67 1023 190
Fragments arranged by size:
1023 251 190 185 69 67
BspEI T'CCGG_A
Cuts at: 0 1730 1785
Size: 1730 55
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1302 1785
Size: 1302 483
BsrFI r'CCGG_y
Cuts at: 0 3 1628 1785
Size: 3 1625 157
Fragments arranged by size:
1625 157 3
BsrGI T'GTAC_A
Cuts at: 0 1004 1785
Size: 1004 781
BssSI C'ACGA_G
Cuts at: 0 612 1785
Size: 612 1173
BstYI r'GATC_y
Cuts at: 0 59 764 941 1405 1727 1785
Size: 59 705 177 464 322 58
Fragments arranged by size:
705 464 322 177 59 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1312 1785
Size: 1312 473
BtsI GCAGTG_nn'
Cuts at: 0 27 521 801 1785
Size: 27 494 280 984
Fragments arranged by size:
984 494 280 27
EaeI y'GGCC_r
Cuts at: 0 597 1037 1785
Size: 597 440 748
Fragments arranged by size:
748 597 440
EarI CTCTTCn'nnn_
Cuts at: 0 515 1741 1785
Size: 515 1226 44
Fragments arranged by size:
1226 515 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 761 1785
Size: 761 1024
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 861 1059 1501 1743 1785
Size: 861 198 442 242 42
Fragments arranged by size:
861 442 242 198 42
EcoO109I rG'GnC_Cy
Cuts at: 0 525 1511 1785
Size: 525 986 274
Fragments arranged by size:
986 525 274
EcoRI G'AATT_C
Cuts at: 0 437 1785
Size: 437 1348
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 111 1785
Size: 111 1674
HaeII r_GCGC'y
Cuts at: 0 1606 1671 1785
Size: 1606 65 114
Fragments arranged by size:
1606 114 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 651 683 1057 1089 1785
Size: 651 32 374 32 696
Fragments arranged by size:
696 651 374 32 32
HincII GTy'rAC
Cuts at: 0 1137 1785
Size: 1137 648
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 711 1785
Size: 711 1074
MslI CAynn'nnrTG
Cuts at: 0 190 850 1228 1785
Size: 190 660 378 557
Fragments arranged by size:
660 557 378 190
MspA1I CmG'CkG
Cuts at: 0 17 156 348 996 1042 1380 1712 1721
Size: 17 139 192 648 46 338 332 9
Cuts at: 1721 1785
Size: 64
Fragments arranged by size:
648 338 332 192 139 64 46 17
9
NaeI GCC'GGC
Cuts at: 0 5 1630 1785
Size: 5 1625 155
Fragments arranged by size:
1625 155 5
NgoMIV G'CCGG_C
Cuts at: 0 3 1628 1785
Size: 3 1625 157
Fragments arranged by size:
1625 157 3
PflMI CCAn_nnn'nTGG
Cuts at: 0 89 1686 1785
Size: 89 1597 99
Fragments arranged by size:
1597 99 89
PfoI T'CCnGG_A
Cuts at: 0 1356 1785
Size: 1356 429
PmlI CAC'GTG
Cuts at: 0 102 1785
Size: 102 1683
PstI C_TGCA'G
Cuts at: 0 16 22 1571 1785
Size: 16 6 1549 214
Fragments arranged by size:
1549 214 16 6
PvuII CAG'CTG
Cuts at: 0 17 996 1380 1712 1785
Size: 17 979 384 332 73
Fragments arranged by size:
979 384 332 73 17
ScaI AGT'ACT
Cuts at: 0 892 1785
Size: 892 893
SfcI C'TryA_G
Cuts at: 0 12 18 1149 1567 1647 1785
Size: 12 6 1131 418 80 138
Fragments arranged by size:
1131 418 138 80 12 6
StyI C'CwwG_G
Cuts at: 0 188 1785
Size: 188 1597
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 410 1021 1785
Size: 410 611 764
Fragments arranged by size:
764 611 410
TatI w'GTAC_w
Cuts at: 0 890 1004 1760 1785
Size: 890 114 756 25
Fragments arranged by size:
890 756 114 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1474 1506 1785
Size: 1474 32 279
Fragments arranged by size:
1474 279 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 513 1326 1785
Size: 513 813 459
Fragments arranged by size:
813 513 459
XmnI GAAnn'nnTTC
Cuts at: 0 301 1785
Size: 301 1484
Enzymes that do cut:
AcuI AflIII AleI AloI AlwNI ApaLI ApoI AvaI
BamHI BanI BanII BbsI BciVI BclI BglII Bme1580I
BmgBI BmrI BpmI BsaAI BsaWI BsaXI BseRI BseYI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI BsrGI BssSI
BstYI BtgZI BtsI EaeI EarI EciI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII MmeI MslI MspA1I
NaeI NgoMIV PflMI PfoI PmlI PstI PvuII ScaI
SfcI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI ApaI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BfrBI BglI BlpI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsaHI BsgI BsiEI BsiWI
BsmI BspHI BsrBI BsrDI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoNI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MscI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI ZraI