Restriction map for
pF1KE1246
(Linear) (Six-base) MAPSORT of: KIEE1246.seq Check: 9899 from: 1 to: 1446
KIEE1246.orf
With 182 enzymes: SgfI *
September 29, 2011 13:09 ..
AccI GT'mk_AC
Cuts at: 0 1419 1446
Size: 1419 27
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 1446
Size: 362 1084
AhdI GACnn_n'nnGTC
Cuts at: 0 1305 1446
Size: 1305 141
AleI CACnn'nnGTG
Cuts at: 0 454 979 1446
Size: 454 525 467
Fragments arranged by size:
525 467 454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 130 1446
Size: 130 1316
AlwNI CAG_nnn'CTG
Cuts at: 0 762 1446
Size: 762 684
ApoI r'AATT_y
Cuts at: 0 365 1446
Size: 365 1081
AvaI C'yCGr_G
Cuts at: 0 677 1243 1446
Size: 677 566 203
Fragments arranged by size:
677 566 203
BanI G'GyrC_C
Cuts at: 0 392 574 1446
Size: 392 182 872
Fragments arranged by size:
872 392 182
BanII G_rGCy'C
Cuts at: 0 8 61 1446
Size: 8 53 1385
Fragments arranged by size:
1385 53 8
BbeI G_GCGC'C
Cuts at: 0 396 1446
Size: 396 1050
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 1172 1365 1446
Size: 134 1038 193 81
Fragments arranged by size:
1038 193 134 81
BclI T'GATC_A
Cuts at: 0 665 1232 1446
Size: 665 567 214
Fragments arranged by size:
665 567 214
BglII A'GATC_T
Cuts at: 0 246 386 1446
Size: 246 140 1060
Fragments arranged by size:
1060 246 140
BlpI GC'TnA_GC
Cuts at: 0 39 1446
Size: 39 1407
Bme1580I G_kGCm'C
Cuts at: 0 577 950 1446
Size: 577 373 496
Fragments arranged by size:
577 496 373
BmgBI CAC'GTC
Cuts at: 0 935 1446
Size: 935 511
BmrI ACTGGGnnnn_n'
Cuts at: 0 1074 1446
Size: 1074 372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 1446
Size: 479 967
Bpu10I CC'TnA_GC
Cuts at: 0 634 1446
Size: 634 812
BsaI GGTCTCn'nnnn_
Cuts at: 0 64 1279 1446
Size: 64 1215 167
Fragments arranged by size:
1215 167 64
BsaAI yAC'GTr
Cuts at: 0 485 1446
Size: 485 961
BsaHI Gr'CG_yC
Cuts at: 0 393 1446
Size: 393 1053
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 1354 1446
Size: 7 1347 92
Fragments arranged by size:
1347 92 7
BseYI C'CCAG_C
Cuts at: 0 259 570 1042 1446
Size: 259 311 472 404
Fragments arranged by size:
472 404 311 259
BsiHKAI G_wGCw'C
Cuts at: 0 461 1446
Size: 461 985
Bsp1286I G_dGCh'C
Cuts at: 0 8 61 461 577 950 1446
Size: 8 53 400 116 373 496
Fragments arranged by size:
496 400 373 116 53 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 845 1405 1446
Size: 845 560 41
Fragments arranged by size:
845 560 41
BsrBI CCG'CTC
Cuts at: 0 653 1037 1446
Size: 653 384 409
Fragments arranged by size:
653 409 384
BsrDI GCAATG_nn'
Cuts at: 0 316 989 1349 1446
Size: 316 673 360 97
Fragments arranged by size:
673 360 316 97
BstAPI GCAn_nnn'nTGC
Cuts at: 0 762 1446
Size: 762 684
BstEII G'GTnAC_C
Cuts at: 0 150 1446
Size: 150 1296
BstYI r'GATC_y
Cuts at: 0 246 386 1446
Size: 246 140 1060
Fragments arranged by size:
1060 246 140
BtgI C'CryG_G
Cuts at: 0 155 977 1211 1446
Size: 155 822 234 235
Fragments arranged by size:
822 235 234 155
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1263 1446
Size: 1263 183
DraI TTT'AAA
Cuts at: 0 96 507 1446
Size: 96 411 939
Fragments arranged by size:
939 411 96
DraIII CAC_nnn'GTG
Cuts at: 0 696 1446
Size: 696 750
DrdI GACnn_nn'nnGTC
Cuts at: 0 595 1293 1446
Size: 595 698 153
Fragments arranged by size:
698 595 153
EaeI y'GGCC_r
Cuts at: 0 118 474 602 1446
Size: 118 356 128 844
Fragments arranged by size:
844 356 128 118
EarI CTCTTCn'nnn_
Cuts at: 0 811 1446
Size: 811 635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 479 1446
Size: 362 117 967
Fragments arranged by size:
967 362 117
EcoO109I rG'GnC_Cy
Cuts at: 0 1201 1446
Size: 1201 245
EcoRV GAT'ATC
Cuts at: 0 1176 1446
Size: 1176 270
HaeII r_GCGC'y
Cuts at: 0 396 762 1446
Size: 396 366 684
Fragments arranged by size:
684 396 366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1291 1323 1446
Size: 1291 32 123
Fragments arranged by size:
1291 123 32
HincII GTy'rAC
Cuts at: 0 783 1420 1446
Size: 783 637 26
Fragments arranged by size:
783 637 26
KasI G'GCGC_C
Cuts at: 0 392 1446
Size: 392 1054
MscI TGG'CCA
Cuts at: 0 476 604 1446
Size: 476 128 842
Fragments arranged by size:
842 476 128
MslI CAynn'nnrTG
Cuts at: 0 184 454 979 1446
Size: 184 270 525 467
Fragments arranged by size:
525 467 270 184
MspA1I CmG'CkG
Cuts at: 0 43 168 263 1446
Size: 43 125 95 1183
Fragments arranged by size:
1183 125 95 43
NarI GG'CG_CC
Cuts at: 0 393 1446
Size: 393 1053
NcoI C'CATG_G
Cuts at: 0 977 1211 1446
Size: 977 234 235
Fragments arranged by size:
977 235 234
NspI r_CATG'y
Cuts at: 0 969 1446
Size: 969 477
PflMI CCAn_nnn'nTGG
Cuts at: 0 144 1446
Size: 144 1302
PmlI CAC'GTG
Cuts at: 0 485 1446
Size: 485 961
PstI C_TGCA'G
Cuts at: 0 167 302 467 562 1446
Size: 167 135 165 95 884
Fragments arranged by size:
884 167 165 135 95
PvuII CAG'CTG
Cuts at: 0 43 168 263 1446
Size: 43 125 95 1183
Fragments arranged by size:
1183 125 95 43
SalI G'TCGA_C
Cuts at: 0 1418 1446
Size: 1418 28
SapI GCTCTTCn'nnn_
Cuts at: 0 811 1446
Size: 811 635
SfcI C'TryA_G
Cuts at: 0 102 163 298 463 558 1446
Size: 102 61 135 165 95 888
Fragments arranged by size:
888 165 135 102 95 61
SfoI GGC'GCC
Cuts at: 0 394 1446
Size: 394 1052
SmaI CCC'GGG
Cuts at: 0 679 1446
Size: 679 767
StyI C'CwwG_G
Cuts at: 0 977 1211 1446
Size: 977 234 235
Fragments arranged by size:
977 235 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 303 547 651 1056 1063 1224 1446
Size: 303 244 104 405 7 161 222
Fragments arranged by size:
405 303 244 222 161 104 7
TatI w'GTAC_w
Cuts at: 0 467 1274 1446
Size: 467 807 172
Fragments arranged by size:
807 467 172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 522 554 1446
Size: 522 32 892
Fragments arranged by size:
892 522 32
XmaI C'CCGG_G
Cuts at: 0 677 1446
Size: 677 769
Enzymes that do cut:
AccI AcuI AhdI AleI AlfI AlwNI ApoI AvaI
BanI BanII BbeI BbsI BclI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BseRI
BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstAPI BstEII
BstYI BtgI BtgZI DraI DraIII DrdI EaeI EarI
Eco57MI EcoO109I EcoRV HaeII Hin4I HincII KasI MscI
MslI MspA1I NarI NcoI NspI PflMI PmlI PstI
PvuII SalI SapI SfcI SfoI SmaI StyI TaqII
TatI TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BfrBI BglI BmtI BplI
BpuEI BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI
BstXI BstZ17I Bsu36I BtsI ClaI CspCI EagI EciI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI
ZraI