Restriction map for
pF1KE1328
(Linear) (Six-base) MAPSORT of: KIEE1328.seq Check: 7665 from: 1 to: 1092
KIEE1328.orf
With 182 enzymes: SgfI *
July 14, 2018 20:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 571 1092
Size: 571 521
Acc65I G'GTAC_C
Cuts at: 0 644 1092
Size: 644 448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 613 1092
Size: 142 471 479
Fragments arranged by size:
479 471 142
AflIII A'CryG_T
Cuts at: 0 68 70 1092
Size: 68 2 1022
Fragments arranged by size:
1022 68 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 553 1092
Size: 553 539
ApaLI G'TGCA_C
Cuts at: 0 528 1092
Size: 528 564
AvaI C'yCGr_G
Cuts at: 0 421 1092
Size: 421 671
BanI G'GyrC_C
Cuts at: 0 199 644 1092
Size: 199 445 448
Fragments arranged by size:
448 445 199
BanII G_rGCy'C
Cuts at: 0 701 1024 1092
Size: 701 323 68
Fragments arranged by size:
701 323 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 231 1092
Size: 56 175 861
Fragments arranged by size:
861 175 56
BbvCI CC'TCA_GC
Cuts at: 0 968 1092
Size: 968 124
BlpI GC'TnA_GC
Cuts at: 0 394 1092
Size: 394 698
Bme1580I G_kGCm'C
Cuts at: 0 202 532 1092
Size: 202 330 560
Fragments arranged by size:
560 330 202
BmrI ACTGGGnnnn_n'
Cuts at: 0 1009 1092
Size: 1009 83
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 715 1092
Size: 400 315 377
Fragments arranged by size:
400 377 315
Bpu10I CC'TnA_GC
Cuts at: 0 968 1092
Size: 968 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 1092
Size: 598 494
BsaI GGTCTCn'nnnn_
Cuts at: 0 926 1035 1092
Size: 926 109 57
Fragments arranged by size:
926 109 57
BsaAI yAC'GTr
Cuts at: 0 71 1092
Size: 71 1021
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 177 538 1092
Size: 177 361 554
Fragments arranged by size:
554 361 177
BseYI C'CCAG_C
Cuts at: 0 21 374 1092
Size: 21 353 718
Fragments arranged by size:
718 353 21
BsiHKAI G_wGCw'C
Cuts at: 0 532 701 1024 1092
Size: 532 169 323 68
Fragments arranged by size:
532 323 169 68
Bsp1286I G_dGCh'C
Cuts at: 0 202 532 701 1024 1092
Size: 202 330 169 323 68
Fragments arranged by size:
330 323 202 169 68
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 545 571 1092
Size: 545 26 521
Fragments arranged by size:
545 521 26
BstXI CCAn_nnnn'nTGG
Cuts at: 0 33 944 1092
Size: 33 911 148
Fragments arranged by size:
911 148 33
BstYI r'GATC_y
Cuts at: 0 6 1092
Size: 6 1086
Bsu36I CC'TnA_GG
Cuts at: 0 189 1092
Size: 189 903
DraI TTT'AAA
Cuts at: 0 834 1092
Size: 834 258
DrdI GACnn_nn'nnGTC
Cuts at: 0 235 1092
Size: 235 857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 616 919 1092
Size: 616 303 173
Fragments arranged by size:
616 303 173
EcoICRI GAG'CTC
Cuts at: 0 699 1022 1092
Size: 699 323 70
Fragments arranged by size:
699 323 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 400 613 715 1092
Size: 142 258 213 102 377
Fragments arranged by size:
377 258 213 142 102
EcoO109I rG'GnC_Cy
Cuts at: 0 1010 1092
Size: 1010 82
HaeII r_GCGC'y
Cuts at: 0 603 1092
Size: 603 489
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 278 310 1092
Size: 278 32 782
Fragments arranged by size:
782 278 32
HincII GTy'rAC
Cuts at: 0 476 1092
Size: 476 616
HindIII A'AGCT_T
Cuts at: 0 664 1092
Size: 664 428
KpnI G_GTAC'C
Cuts at: 0 648 1092
Size: 648 444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 1092
Size: 925 167
MspA1I CmG'CkG
Cuts at: 0 315 954 1092
Size: 315 639 138
Fragments arranged by size:
639 315 138
NspI r_CATG'y
Cuts at: 0 84 1092
Size: 84 1008
PasI CC'CwG_GG
Cuts at: 0 465 756 1092
Size: 465 291 336
Fragments arranged by size:
465 336 291
PfoI T'CCnGG_A
Cuts at: 0 296 893 1024 1092
Size: 296 597 131 68
Fragments arranged by size:
597 296 131 68
PmlI CAC'GTG
Cuts at: 0 71 1092
Size: 71 1021
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 1092
Size: 538 32 522
Fragments arranged by size:
538 522 32
PpuMI rG'GwC_Cy
Cuts at: 0 1010 1092
Size: 1010 82
PstI C_TGCA'G
Cuts at: 0 542 1092
Size: 542 550
PvuII CAG'CTG
Cuts at: 0 315 954 1092
Size: 315 639 138
Fragments arranged by size:
639 315 138
SacI G_AGCT'C
Cuts at: 0 701 1024 1092
Size: 701 323 68
Fragments arranged by size:
701 323 68
SanDI GG'GwC_CC
Cuts at: 0 1010 1092
Size: 1010 82
SexAI A'CCwGG_T
Cuts at: 0 230 1092
Size: 230 862
SfcI C'TryA_G
Cuts at: 0 538 577 911 1092
Size: 538 39 334 181
Fragments arranged by size:
538 334 181 39
SmlI C'TyrA_G
Cuts at: 0 613 1092
Size: 613 479
SphI G_CATG'C
Cuts at: 0 84 1092
Size: 84 1008
StuI AGG'CCT
Cuts at: 0 885 973 1092
Size: 885 88 119
Fragments arranged by size:
885 119 88
StyI C'CwwG_G
Cuts at: 0 167 1092
Size: 167 925
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 913 1092
Size: 913 179
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1022 1092
Size: 1022 70
Enzymes that do cut:
AarI Acc65I AcuI AflIII AlfI ApaLI AvaI BanI
BanII BbsI BbvCI BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BseRI BseYI BsiHKAI Bsp1286I BspMI
BstXI BstYI Bsu36I DraI DrdI EciI EcoICRI Eco57MI
EcoO109I HaeII Hin4I HincII HindIII KpnI MmeI MspA1I
NspI PasI PfoI PmlI PpiI PpuMI PstI PvuII
SacI SanDI SexAI SfcI SmlI SphI StuI StyI
TaqII XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AloI AlwNI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmtI BplI BsaBI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraIII EaeI
EagI EarI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HpaI KasI MfeI MluI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PmeI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI