Restriction map for
pF1KE1345
(Linear) (Six-base) MAPSORT of: KIEE1345.seq Check: 7543 from: 1 to: 792
KIEE1345.orf
With 182 enzymes: SgfI *
February 23, 2015 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 399 792
Size: 399 393
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 436 580 792
Size: 313 123 144 212
Fragments arranged by size:
313 212 144 123
AflII C'TTAA_G
Cuts at: 0 112 792
Size: 112 680
AhdI GACnn_n'nnGTC
Cuts at: 0 400 792
Size: 400 392
AlwNI CAG_nnn'CTG
Cuts at: 0 270 279 414 444 792
Size: 270 9 135 30 348
Fragments arranged by size:
348 270 135 30 9
AvaI C'yCGr_G
Cuts at: 0 50 792
Size: 50 742
BclI T'GATC_A
Cuts at: 0 581 792
Size: 581 211
BlpI GC'TnA_GC
Cuts at: 0 481 778 792
Size: 481 297 14
Fragments arranged by size:
481 297 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 319 454 792
Size: 319 135 338
Fragments arranged by size:
338 319 135
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 729 792
Size: 729 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 618 792
Size: 301 317 174
Fragments arranged by size:
317 301 174
Bpu10I CC'TnA_GC
Cuts at: 0 538 792
Size: 538 254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 792
Size: 580 212
BsaI GGTCTCn'nnnn_
Cuts at: 0 161 792
Size: 161 631
BsaBI GATnn'nnATC
Cuts at: 0 181 792
Size: 181 611
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 373 403 792
Size: 373 30 389
Fragments arranged by size:
389 373 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 397 792
Size: 202 195 395
Fragments arranged by size:
395 202 195
BsiHKAI G_wGCw'C
Cuts at: 0 482 792
Size: 482 310
Bsp1286I G_dGCh'C
Cuts at: 0 482 792
Size: 482 310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 278 399 792
Size: 179 99 121 393
Fragments arranged by size:
393 179 121 99
BstEII G'GTnAC_C
Cuts at: 0 330 792
Size: 330 462
BtgI C'CryG_G
Cuts at: 0 734 792
Size: 734 58
DraIII CAC_nnn'GTG
Cuts at: 0 491 792
Size: 491 301
EaeI y'GGCC_r
Cuts at: 0 737 792
Size: 737 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 313 436 580 618 792
Size: 301 12 123 144 38 174
Fragments arranged by size:
301 174 144 123 38 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 528 792
Size: 528 264
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 743 792
Size: 743 49
HaeII r_GCGC'y
Cuts at: 0 538 792
Size: 538 254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 386 418 792
Size: 172 32 182 32 374
Fragments arranged by size:
374 182 172 32 32
MscI TGG'CCA
Cuts at: 0 739 792
Size: 739 53
NcoI C'CATG_G
Cuts at: 0 734 792
Size: 734 58
PasI CC'CwG_GG
Cuts at: 0 645 792
Size: 645 147
PflMI CCAn_nnn'nTGG
Cuts at: 0 444 792
Size: 444 348
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 613 645 792
Size: 613 32 147
Fragments arranged by size:
613 147 32
PstI C_TGCA'G
Cuts at: 0 99 275 532 792
Size: 99 176 257 260
Fragments arranged by size:
260 257 176 99
SbfI CC_TGCA'GG
Cuts at: 0 275 792
Size: 275 517
SfcI C'TryA_G
Cuts at: 0 95 271 528 792
Size: 95 176 257 264
Fragments arranged by size:
264 257 176 95
SmlI C'TyrA_G
Cuts at: 0 112 595 792
Size: 112 483 197
Fragments arranged by size:
483 197 112
SspI AAT'ATT
Cuts at: 0 70 792
Size: 70 722
StuI AGG'CCT
Cuts at: 0 310 792
Size: 310 482
StyI C'CwwG_G
Cuts at: 0 20 734 792
Size: 20 714 58
Fragments arranged by size:
714 58 20
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 642 792
Size: 642 150
Enzymes that do cut:
AarI AcuI AflII AhdI AlwNI AvaI BclI BlpI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI
BseRI BsiHKAI Bsp1286I BspMI BstEII BtgI DraIII EaeI
Eco57MI EcoNI FalI HaeII Hin4I MscI NcoI PasI
PflMI PpiI PstI SbfI SfcI SmlI SspI StuI
StyI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI BmtI
BsaAI BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EagI EarI EciI
EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI