Restriction map for
pF1KE1351
(Linear) (Six-base) MAPSORT of: KIEE1351.seq Check: 123 from: 1 to: 1911
KIEE1351.orf
With 182 enzymes: SgfI *
April 12, 2013 15:25 ..
Acc65I G'GTAC_C
Cuts at: 0 462 1911
Size: 462 1449
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1545 1911
Size: 919 626 366
Fragments arranged by size:
919 626 366
AflII C'TTAA_G
Cuts at: 0 858 1911
Size: 858 1053
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 716 748 772 804 1911
Size: 716 32 24 32 1107
Fragments arranged by size:
1107 716 32 32 24
AlwNI CAG_nnn'CTG
Cuts at: 0 330 942 1080 1911
Size: 330 612 138 831
Fragments arranged by size:
831 612 330 138
ApoI r'AATT_y
Cuts at: 0 333 1615 1911
Size: 333 1282 296
Fragments arranged by size:
1282 333 296
AvaI C'yCGr_G
Cuts at: 0 1393 1911
Size: 1393 518
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1055 1088 1911
Size: 1055 33 823
Fragments arranged by size:
1055 823 33
BanI G'GyrC_C
Cuts at: 0 462 474 802 1911
Size: 462 12 328 1109
Fragments arranged by size:
1109 462 328 12
BanII G_rGCy'C
Cuts at: 0 674 1315 1359 1911
Size: 674 641 44 552
Fragments arranged by size:
674 641 552 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 126 346 920 1911
Size: 126 220 574 991
Fragments arranged by size:
991 574 220 126
BciVI GTATCCnnnnn_n'
Cuts at: 0 1056 1708 1911
Size: 1056 652 203
Fragments arranged by size:
1056 652 203
BglI GCCn_nnn'nGGC
Cuts at: 0 1141 1911
Size: 1141 770
BmrI ACTGGGnnnn_n'
Cuts at: 0 1482 1911
Size: 1482 429
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 849 1731 1911
Size: 849 882 180
Fragments arranged by size:
882 849 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1259 1911
Size: 1259 652
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1911
Size: 757 1154
BsaAI yAC'GTr
Cuts at: 0 1777 1911
Size: 1777 134
BsaWI w'CCGG_w
Cuts at: 0 575 1911
Size: 575 1336
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 772 802 1701 1731 1911
Size: 538 30 204 30 899 30 180
Fragments arranged by size:
899 538 204 180 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 544 559 760 1069 1911
Size: 544 15 201 309 842
Fragments arranged by size:
842 544 309 201 15
BseYI C'CCAG_C
Cuts at: 0 1089 1358 1544 1911
Size: 1089 269 186 367
Fragments arranged by size:
1089 367 269 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 1501 1523 1911
Size: 805 696 22 388
Fragments arranged by size:
805 696 388 22
BsiEI CG_ry'CG
Cuts at: 0 17 1149 1911
Size: 17 1132 762
Fragments arranged by size:
1132 762 17
BsmI GAATG_Cn'
Cuts at: 0 513 1911
Size: 513 1398
Bsp1286I G_dGCh'C
Cuts at: 0 674 1315 1359 1911
Size: 674 641 44 552
Fragments arranged by size:
674 641 552 44
BspEI T'CCGG_A
Cuts at: 0 575 1911
Size: 575 1336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 180 1911
Size: 180 1731
BsrBI CCG'CTC
Cuts at: 0 1892 1911
Size: 1892 19
BsrFI r'CCGG_y
Cuts at: 0 1148 1654 1911
Size: 1148 506 257
Fragments arranged by size:
1148 506 257
BsrGI T'GTAC_A
Cuts at: 0 1439 1911
Size: 1439 472
BstYI r'GATC_y
Cuts at: 0 1578 1911
Size: 1578 333
Bsu36I CC'TnA_GG
Cuts at: 0 1481 1911
Size: 1481 430
BtgI C'CryG_G
Cuts at: 0 262 1190 1911
Size: 262 928 721
Fragments arranged by size:
928 721 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 22 1824 1911
Size: 22 1802 87
Fragments arranged by size:
1802 87 22
BtsI GCAGTG_nn'
Cuts at: 0 614 1911
Size: 614 1297
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1172 1207 1911
Size: 1172 35 704
Fragments arranged by size:
1172 704 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1294 1911
Size: 1294 617
EaeI y'GGCC_r
Cuts at: 0 98 314 401 432 1911
Size: 98 216 87 31 1479
Fragments arranged by size:
1479 216 98 87 31
EarI CTCTTCn'nnn_
Cuts at: 0 447 1046 1431 1753 1911
Size: 447 599 385 322 158
Fragments arranged by size:
599 447 385 322 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1167 1911
Size: 1167 744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1259 1545 1911
Size: 919 340 286 366
Fragments arranged by size:
919 366 340 286
EcoNI CCTnn'n_nnAGG
Cuts at: 0 379 1911
Size: 379 1532
EcoO109I rG'GnC_Cy
Cuts at: 0 54 483 987 1105 1911
Size: 54 429 504 118 806
Fragments arranged by size:
806 504 429 118 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 936 968 1686 1701 1718 1733
Size: 622 32 282 32 718 15 17 15
Cuts at: 1733 1911
Size: 178
Fragments arranged by size:
718 622 282 178 32 32 17 15
15
HindIII A'AGCT_T
Cuts at: 0 253 1911
Size: 253 1658
KpnI G_GTAC'C
Cuts at: 0 466 1911
Size: 466 1445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1911
Size: 758 1153
MscI TGG'CCA
Cuts at: 0 100 403 434 1911
Size: 100 303 31 1477
Fragments arranged by size:
1477 303 100 31
MslI CAynn'nnrTG
Cuts at: 0 1795 1911
Size: 1795 116
MspA1I CmG'CkG
Cuts at: 0 47 1911
Size: 47 1864
NaeI GCC'GGC
Cuts at: 0 1656 1911
Size: 1656 255
NcoI C'CATG_G
Cuts at: 0 262 1911
Size: 262 1649
NdeI CA'TA_TG
Cuts at: 0 969 1911
Size: 969 942
NgoMIV G'CCGG_C
Cuts at: 0 1654 1911
Size: 1654 257
NspI r_CATG'y
Cuts at: 0 129 1911
Size: 129 1782
PflMI CCAn_nnn'nTGG
Cuts at: 0 452 849 1911
Size: 452 397 1062
Fragments arranged by size:
1062 452 397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 772 804 811 843 1911
Size: 772 32 7 32 1068
Fragments arranged by size:
1068 772 32 32 7
PpuMI rG'GwC_Cy
Cuts at: 0 54 483 987 1105 1911
Size: 54 429 504 118 806
Fragments arranged by size:
806 504 429 118 54
PshAI GACnn'nnGTC
Cuts at: 0 1243 1911
Size: 1243 668
PstI C_TGCA'G
Cuts at: 0 926 1911
Size: 926 985
SanDI GG'GwC_CC
Cuts at: 0 1105 1911
Size: 1105 806
ScaI AGT'ACT
Cuts at: 0 1640 1911
Size: 1640 271
SfcI C'TryA_G
Cuts at: 0 591 922 1458 1911
Size: 591 331 536 453
Fragments arranged by size:
591 536 453 331
SmaI CCC'GGG
Cuts at: 0 1395 1911
Size: 1395 516
SmlI C'TyrA_G
Cuts at: 0 772 858 1911
Size: 772 86 1053
Fragments arranged by size:
1053 772 86
SphI G_CATG'C
Cuts at: 0 129 1911
Size: 129 1782
StyI C'CwwG_G
Cuts at: 0 262 467 902 1610 1911
Size: 262 205 435 708 301
Fragments arranged by size:
708 435 301 262 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1055 1257 1911
Size: 1055 202 654
Fragments arranged by size:
1055 654 202
TatI w'GTAC_w
Cuts at: 0 533 1400 1439 1638 1911
Size: 533 867 39 199 273
Fragments arranged by size:
867 533 273 199 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 602 1097 1129 1220 1252 1911
Size: 538 32 32 495 32 91 32 659
Fragments arranged by size:
659 538 495 91 32 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 427 1911
Size: 427 1484
XmaI C'CCGG_G
Cuts at: 0 1393 1911
Size: 1393 518
XmnI GAAnn'nnTTC
Cuts at: 0 255 826 1911
Size: 255 571 1085
Fragments arranged by size:
1085 571 255
Enzymes that do cut:
Acc65I AcuI AflII AloI AlwNI ApoI AvaI BaeI
BanI BanII BbsI BciVI BglI BmrI BplI BpmI
BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsmI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I Hin4I HindIII KpnI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI
PflMI PpiI PpuMI PshAI PstI SanDI ScaI SfcI
SmaI SmlI SphI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BbeI BbvCI BcgI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmtI Bpu10I BsaI BsaBI BsaHI BsiHKAI
BsiWI BsmBI BspHI BsrDI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I ClaI DraI DraIII EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI MfeI MluI NarI NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XhoI ZraI