Restriction map for
pF1KE1355
(Linear) (Six-base) MAPSORT of: KIEE1355.seq Check: 1259 from: 1 to: 846
KIEE1355.orf
With 182 enzymes: SgfI *
November 21, 2014 11:13 ..
AatII G_ACGT'C
Cuts at: 0 515 554 846
Size: 515 39 292
Fragments arranged by size:
515 292 39
AccI GT'mk_AC
Cuts at: 0 515 846
Size: 515 331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 846
Size: 88 758
AfeI AGC'GCT
Cuts at: 0 301 663 846
Size: 301 362 183
Fragments arranged by size:
362 301 183
AhdI GACnn_n'nnGTC
Cuts at: 0 330 846
Size: 330 516
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 31 846
Size: 31 815
AlwNI CAG_nnn'CTG
Cuts at: 0 150 648 846
Size: 150 498 198
Fragments arranged by size:
498 198 150
AvaI C'yCGr_G
Cuts at: 0 474 846
Size: 474 372
BanI G'GyrC_C
Cuts at: 0 733 846
Size: 733 113
BanII G_rGCy'C
Cuts at: 0 374 846
Size: 374 472
BbeI G_GCGC'C
Cuts at: 0 737 846
Size: 737 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 556 590 846
Size: 556 34 256
Fragments arranged by size:
556 256 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 286 597 846
Size: 286 311 249
Fragments arranged by size:
311 286 249
Bpu10I CC'TnA_GC
Cuts at: 0 151 846
Size: 151 695
BsaI GGTCTCn'nnnn_
Cuts at: 0 97 588 846
Size: 97 491 258
Fragments arranged by size:
491 258 97
BsaAI yAC'GTr
Cuts at: 0 261 846
Size: 261 585
BsaHI Gr'CG_yC
Cuts at: 0 512 551 734 846
Size: 512 39 183 112
Fragments arranged by size:
512 183 112 39
BseYI C'CCAG_C
Cuts at: 0 18 846
Size: 18 828
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 846
Size: 163 683
Bsp1286I G_dGCh'C
Cuts at: 0 374 846
Size: 374 472
BsrGI T'GTAC_A
Cuts at: 0 314 846
Size: 314 532
BstXI CCAn_nnnn'nTGG
Cuts at: 0 97 846
Size: 97 749
BstYI r'GATC_y
Cuts at: 0 146 846
Size: 146 700
BtgI C'CryG_G
Cuts at: 0 206 846
Size: 206 640
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 150 846
Size: 150 696
DraIII CAC_nnn'GTG
Cuts at: 0 261 846
Size: 261 585
DrdI GACnn_nn'nnGTC
Cuts at: 0 111 846
Size: 111 735
EarI CTCTTCn'nnn_
Cuts at: 0 761 846
Size: 761 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 607 846
Size: 607 239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 846
Size: 88 758
EcoNI CCTnn'n_nnAGG
Cuts at: 0 49 175 232 699 846
Size: 49 126 57 467 147
Fragments arranged by size:
467 147 126 57 49
HaeII r_GCGC'y
Cuts at: 0 28 303 665 737 846
Size: 28 275 362 72 109
Fragments arranged by size:
362 275 109 72 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 486 518 846
Size: 316 32 138 32 328
Fragments arranged by size:
328 316 138 32 32
KasI G'GCGC_C
Cuts at: 0 733 846
Size: 733 113
MslI CAynn'nnrTG
Cuts at: 0 559 846
Size: 559 287
NarI GG'CG_CC
Cuts at: 0 734 846
Size: 734 112
NspI r_CATG'y
Cuts at: 0 469 846
Size: 469 377
PfoI T'CCnGG_A
Cuts at: 0 176 846
Size: 176 670
PstI C_TGCA'G
Cuts at: 0 53 586 846
Size: 53 533 260
Fragments arranged by size:
533 260 53
SapI GCTCTTCn'nnn_
Cuts at: 0 761 846
Size: 761 85
SexAI A'CCwGG_T
Cuts at: 0 326 846
Size: 326 520
SfcI C'TryA_G
Cuts at: 0 49 582 846
Size: 49 533 264
Fragments arranged by size:
533 264 49
SfoI GGC'GCC
Cuts at: 0 735 846
Size: 735 111
SmaI CCC'GGG
Cuts at: 0 476 846
Size: 476 370
SphI G_CATG'C
Cuts at: 0 469 846
Size: 469 377
StuI AGG'CCT
Cuts at: 0 321 846
Size: 321 525
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 696 846
Size: 696 150
TatI w'GTAC_w
Cuts at: 0 314 846
Size: 314 532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 778 846
Size: 778 68
XmaI C'CCGG_G
Cuts at: 0 474 846
Size: 474 372
ZraI GAC'GTC
Cuts at: 0 513 552 846
Size: 513 39 294
Fragments arranged by size:
513 294 39
Enzymes that do cut:
AatII AccI AcuI AfeI AhdI AlfI AlwNI AvaI
BanI BanII BbeI BcgI BmrI Bpu10I BsaI BsaAI
BsaHI BseYI BsgI Bsp1286I BsrGI BstXI BstYI BtgI
BtgZI DraIII DrdI EarI EciI Eco57MI EcoNI HaeII
Hin4I KasI MslI NarI NspI PfoI PstI SapI
SexAI SfcI SfoI SmaI SphI StuI TaqII TatI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AleI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmtI BplI BpmI BpuEI BsaBI
BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI EaeI EagI EcoICRI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StyI SwaI TstI Tth111I XbaI XhoI
XmnI