Restriction map for
pF1KE1360
(Linear) (Six-base) MAPSORT of: KIEE1360.seq Check: 5677 from: 1 to: 717
KIEE1360.orf
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 717
Size: 42 675
ApaI G_GGCC'C
Cuts at: 0 186 717
Size: 186 531
ApoI r'AATT_y
Cuts at: 0 88 717
Size: 88 629
BamHI G'GATC_C
Cuts at: 0 371 717
Size: 371 346
BanII G_rGCy'C
Cuts at: 0 186 605 717
Size: 186 419 112
Fragments arranged by size:
419 186 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 48 717
Size: 48 669
BbvCI CC'TCA_GC
Cuts at: 0 83 717
Size: 83 634
BfrBI ATG'CAT
Cuts at: 0 353 717
Size: 353 364
BlpI GC'TnA_GC
Cuts at: 0 641 717
Size: 641 76
Bme1580I G_kGCm'C
Cuts at: 0 186 717
Size: 186 531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 691 717
Size: 460 231 26
Fragments arranged by size:
460 231 26
Bpu10I CC'TnA_GC
Cuts at: 0 25 83 604 717
Size: 25 58 521 113
Fragments arranged by size:
521 113 58 25
BsiHKAI G_wGCw'C
Cuts at: 0 605 717
Size: 605 112
BsmI GAATG_Cn'
Cuts at: 0 353 456 717
Size: 353 103 261
Fragments arranged by size:
353 261 103
Bsp1286I G_dGCh'C
Cuts at: 0 186 605 717
Size: 186 419 112
Fragments arranged by size:
419 186 112
BsrBI CCG'CTC
Cuts at: 0 29 717
Size: 29 688
BstXI CCAn_nnnn'nTGG
Cuts at: 0 332 618 717
Size: 332 286 99
Fragments arranged by size:
332 286 99
BstYI r'GATC_y
Cuts at: 0 371 717
Size: 371 346
Bsu36I CC'TnA_GG
Cuts at: 0 625 717
Size: 625 92
BtgI C'CryG_G
Cuts at: 0 611 717
Size: 611 106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 29 717
Size: 29 688
EaeI y'GGCC_r
Cuts at: 0 2 524 717
Size: 2 522 193
Fragments arranged by size:
522 193 2
EcoICRI GAG'CTC
Cuts at: 0 603 717
Size: 603 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 460 691 717
Size: 42 418 231 26
Fragments arranged by size:
418 231 42 26
EcoO109I rG'GnC_Cy
Cuts at: 0 183 629 717
Size: 183 446 88
Fragments arranged by size:
446 183 88
EcoRI G'AATT_C
Cuts at: 0 88 717
Size: 88 629
HincII GTy'rAC
Cuts at: 0 684 717
Size: 684 33
HindIII A'AGCT_T
Cuts at: 0 473 717
Size: 473 244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 717
Size: 399 318
MscI TGG'CCA
Cuts at: 0 4 526 717
Size: 4 522 191
Fragments arranged by size:
522 191 4
MslI CAynn'nnrTG
Cuts at: 0 358 717
Size: 358 359
NcoI C'CATG_G
Cuts at: 0 611 717
Size: 611 106
NsiI A_TGCA'T
Cuts at: 0 355 717
Size: 355 362
PasI CC'CwG_GG
Cuts at: 0 283 717
Size: 283 434
PfoI T'CCnGG_A
Cuts at: 0 74 425 717
Size: 74 351 292
Fragments arranged by size:
351 292 74
PspOMI G'GGCC_C
Cuts at: 0 182 717
Size: 182 535
SacI G_AGCT'C
Cuts at: 0 605 717
Size: 605 112
ScaI AGT'ACT
Cuts at: 0 499 717
Size: 499 218
SfcI C'TryA_G
Cuts at: 0 96 717
Size: 96 621
StuI AGG'CCT
Cuts at: 0 532 717
Size: 532 185
StyI C'CwwG_G
Cuts at: 0 218 527 536 566 611 717
Size: 218 309 9 30 45 106
Fragments arranged by size:
309 218 106 45 30 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 231 717
Size: 231 486
TatI w'GTAC_w
Cuts at: 0 497 717
Size: 497 220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 717
Size: 179 32 506
Fragments arranged by size:
506 179 32
XmnI GAAnn'nnTTC
Cuts at: 0 70 717
Size: 70 647
Enzymes that do cut:
AcuI ApaI ApoI BamHI BanII BbsI BbvCI BfrBI
BlpI Bme1580I BpmI Bpu10I BsiHKAI BsmI Bsp1286I BsrBI
BstXI BstYI Bsu36I BtgI CspCI EaeI EcoICRI Eco57MI
EcoO109I EcoRI HincII HindIII MmeI MscI MslI NcoI
NsiI PasI PfoI PspOMI SacI ScaI SfcI StuI
StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanI BbeI BcgI
BciVI BclI BglI BglII BmgBI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI BtsI ClaI DraI DraIII DrdI EagI
EarI EciI EcoNI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HpaI KasI KpnI MfeI MluI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NspI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI