Restriction map for
pF1KE1398
(Linear) (Six-base) MAPSORT of: KIEE1398.seq Check: 973 from: 1 to: 3306
KIEE1398.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AccI GT'mk_AC
Cuts at: 0 1010 1550 3306
Size: 1010 540 1756
Fragments arranged by size:
1756 1010 540
AclI AA'CG_TT
Cuts at: 0 3004 3306
Size: 3004 302
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 433 3306
Size: 216 217 2873
Fragments arranged by size:
2873 217 216
AfeI AGC'GCT
Cuts at: 0 229 3306
Size: 229 3077
AflII C'TTAA_G
Cuts at: 0 2641 3306
Size: 2641 665
AflIII A'CryG_T
Cuts at: 0 1541 1745 2249 3306
Size: 1541 204 504 1057
Fragments arranged by size:
1541 1057 504 204
AhdI GACnn_n'nnGTC
Cuts at: 0 1017 1452 1512 3306
Size: 1017 435 60 1794
Fragments arranged by size:
1794 1017 435 60
AlwNI CAG_nnn'CTG
Cuts at: 0 3078 3306
Size: 3078 228
ApoI r'AATT_y
Cuts at: 0 1180 1276 1303 1435 1856 2220 2234 3115
Size: 1180 96 27 132 421 364 14 881
Cuts at: 3115 3174 3306
Size: 59 132
Fragments arranged by size:
1180 881 421 364 132 132 96 59
27 14
AvaI C'yCGr_G
Cuts at: 0 58 1999 3306
Size: 58 1941 1307
Fragments arranged by size:
1941 1307 58
BanI G'GyrC_C
Cuts at: 0 937 952 3306
Size: 937 15 2354
Fragments arranged by size:
2354 937 15
BanII G_rGCy'C
Cuts at: 0 59 1952 3236 3306
Size: 59 1893 1284 70
Fragments arranged by size:
1893 1284 70 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 2988 3164 3306
Size: 2988 176 142
Fragments arranged by size:
2988 176 142
BbvCI CC'TCA_GC
Cuts at: 0 1610 3025 3306
Size: 1610 1415 281
Fragments arranged by size:
1610 1415 281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1990 2024 3306
Size: 1990 34 1282
Fragments arranged by size:
1990 1282 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3023 3306
Size: 3023 283
BclI T'GATC_A
Cuts at: 0 122 3306
Size: 122 3184
BglII A'GATC_T
Cuts at: 0 2364 2586 3306
Size: 2364 222 720
Fragments arranged by size:
2364 720 222
Bme1580I G_kGCm'C
Cuts at: 0 955 3306
Size: 955 2351
BmgBI CAC'GTC
Cuts at: 0 383 1449 2250 3306
Size: 383 1066 801 1056
Fragments arranged by size:
1066 1056 801 383
BmrI ACTGGGnnnn_n'
Cuts at: 0 2146 3306
Size: 2146 1160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 925 1844 3306
Size: 925 919 1462
Fragments arranged by size:
1462 925 919
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 537 3306
Size: 340 197 2769
Fragments arranged by size:
2769 340 197
Bpu10I CC'TnA_GC
Cuts at: 0 256 1610 3025 3306
Size: 256 1354 1415 281
Fragments arranged by size:
1415 1354 281 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2510 3306
Size: 2510 796
BsaAI yAC'GTr
Cuts at: 0 2140 3306
Size: 2140 1166
BsaBI GATnn'nnATC
Cuts at: 0 2042 2650 3306
Size: 2042 608 656
Fragments arranged by size:
2042 656 608
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3085 3115 3306
Size: 3085 30 191
Fragments arranged by size:
3085 191 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 1788 3276 3306
Size: 181 1607 1488 30
Fragments arranged by size:
1607 1488 181 30
BseYI C'CCAG_C
Cuts at: 0 1141 3306
Size: 1141 2165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1512 1720 3306
Size: 1512 208 1586
Fragments arranged by size:
1586 1512 208
BsiHKAI G_wGCw'C
Cuts at: 0 1487 1952 3306
Size: 1487 465 1354
Fragments arranged by size:
1487 1354 465
Bsp1286I G_dGCh'C
Cuts at: 0 59 955 1487 1952 3236 3306
Size: 59 896 532 465 1284 70
Fragments arranged by size:
1284 896 532 465 70 59
BspHI T'CATG_A
Cuts at: 0 2046 3306
Size: 2046 1260
BsrBI CCG'CTC
Cuts at: 0 1671 3306
Size: 1671 1635
BsrDI GCAATG_nn'
Cuts at: 0 369 1189 1913 3306
Size: 369 820 724 1393
Fragments arranged by size:
1393 820 724 369
BsrGI T'GTAC_A
Cuts at: 0 582 725 3306
Size: 582 143 2581
Fragments arranged by size:
2581 582 143
BstBI TT'CG_AA
Cuts at: 0 2910 3306
Size: 2910 396
BstEII G'GTnAC_C
Cuts at: 0 1590 3306
Size: 1590 1716
BstXI CCAn_nnnn'nTGG
Cuts at: 0 510 1726 3306
Size: 510 1216 1580
Fragments arranged by size:
1580 1216 510
BstYI r'GATC_y
Cuts at: 0 1320 2364 2496 2586 3306
Size: 1320 1044 132 90 720
Fragments arranged by size:
1320 1044 720 132 90
Bsu36I CC'TnA_GG
Cuts at: 0 1325 3306
Size: 1325 1981
BtgI C'CryG_G
Cuts at: 0 161 286 600 972 1835 2783 3306
Size: 161 125 314 372 863 948 523
Fragments arranged by size:
948 863 523 372 314 161 125
BtsI GCAGTG_nn'
Cuts at: 0 413 1524 3306
Size: 413 1111 1782
Fragments arranged by size:
1782 1111 413
DraI TTT'AAA
Cuts at: 0 2682 3114 3306
Size: 2682 432 192
Fragments arranged by size:
2682 432 192
EaeI y'GGCC_r
Cuts at: 0 1728 3306
Size: 1728 1578
EarI CTCTTCn'nnn_
Cuts at: 0 1023 1631 2708 3306
Size: 1023 608 1077 598
Fragments arranged by size:
1077 1023 608 598
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 604 3306
Size: 604 2702
EcoICRI GAG'CTC
Cuts at: 0 1950 3306
Size: 1950 1356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 340 433 537 3306
Size: 216 124 93 104 2769
Fragments arranged by size:
2769 216 124 104 93
EcoNI CCTnn'n_nnAGG
Cuts at: 0 539 2203 3227 3283 3306
Size: 539 1664 1024 56 23
Fragments arranged by size:
1664 1024 539 56 23
EcoO109I rG'GnC_Cy
Cuts at: 0 1329 2757 3306
Size: 1329 1428 549
Fragments arranged by size:
1428 1329 549
EcoRI G'AATT_C
Cuts at: 0 1276 2234 3306
Size: 1276 958 1072
Fragments arranged by size:
1276 1072 958
EcoRV GAT'ATC
Cuts at: 0 1047 3306
Size: 1047 2259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 785 3306
Size: 785 2521
HaeII r_GCGC'y
Cuts at: 0 198 231 3306
Size: 198 33 3075
Fragments arranged by size:
3075 198 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 338 367 370 399 427 459 739 771
Size: 338 29 3 29 28 32 280 32
Cuts at: 771 1003 1035 1438 1470 1498 1530 1638 1670
Size: 232 32 403 32 28 32 108 32
Cuts at: 1670 2061 2093 2788 2820 3306
Size: 391 32 695 32 486
Fragments arranged by size:
695 486 403 391 338 280 232 108
32 32 32 32 32 32 32 32
29 29 28 28 3
HincII GTy'rAC
Cuts at: 0 836 3306
Size: 836 2470
HpaI GTT'AAC
Cuts at: 0 836 3306
Size: 836 2470
MfeI C'AATT_G
Cuts at: 0 1478 3122 3306
Size: 1478 1644 184
Fragments arranged by size:
1644 1478 184
MscI TGG'CCA
Cuts at: 0 1730 3306
Size: 1730 1576
MslI CAynn'nnrTG
Cuts at: 0 70 849 1490 2567 2747 3306
Size: 70 779 641 1077 180 559
Fragments arranged by size:
1077 779 641 559 180 70
MspA1I CmG'CkG
Cuts at: 0 163 974 3306
Size: 163 811 2332
Fragments arranged by size:
2332 811 163
NcoI C'CATG_G
Cuts at: 0 286 600 1835 3306
Size: 286 314 1235 1471
Fragments arranged by size:
1471 1235 314 286
NspI r_CATG'y
Cuts at: 0 1545 1749 3306
Size: 1545 204 1557
Fragments arranged by size:
1557 1545 204
PciI A'CATG_T
Cuts at: 0 1541 1745 3306
Size: 1541 204 1561
Fragments arranged by size:
1561 1541 204
PflMI CCAn_nnn'nTGG
Cuts at: 0 386 3306
Size: 386 2920
PfoI T'CCnGG_A
Cuts at: 0 1982 2418 2753 3089 3306
Size: 1982 436 335 336 217
Fragments arranged by size:
1982 436 336 335 217
PpuMI rG'GwC_Cy
Cuts at: 0 1329 2757 3306
Size: 1329 1428 549
Fragments arranged by size:
1428 1329 549
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1139 1171 3306
Size: 1139 32 2135
Fragments arranged by size:
2135 1139 32
PstI C_TGCA'G
Cuts at: 0 2513 3306
Size: 2513 793
SacI G_AGCT'C
Cuts at: 0 1952 3306
Size: 1952 1354
SacII CC_GC'GG
Cuts at: 0 164 975 3306
Size: 164 811 2331
Fragments arranged by size:
2331 811 164
SexAI A'CCwGG_T
Cuts at: 0 2933 3306
Size: 2933 373
SfcI C'TryA_G
Cuts at: 0 685 818 2509 2593 3306
Size: 685 133 1691 84 713
Fragments arranged by size:
1691 713 685 133 84
SmlI C'TyrA_G
Cuts at: 0 2525 2641 3306
Size: 2525 116 665
Fragments arranged by size:
2525 665 116
SspI AAT'ATT
Cuts at: 0 1979 3306
Size: 1979 1327
StyI C'CwwG_G
Cuts at: 0 286 600 1734 1835 1969 3223 3306
Size: 286 314 1134 101 134 1254 83
Fragments arranged by size:
1254 1134 314 286 134 101 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 240 544 839 1007 2735 3306
Size: 240 304 295 168 1728 571
Fragments arranged by size:
1728 571 304 295 240 168
TatI w'GTAC_w
Cuts at: 0 582 725 1462 3146 3306
Size: 582 143 737 1684 160
Fragments arranged by size:
1684 737 582 160 143
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1379 1411 3306
Size: 1379 32 1895
Fragments arranged by size:
1895 1379 32
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AflIII AhdI AlwNI
ApoI AvaI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BsrBI BsrDI BsrGI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HpaI MfeI MscI MslI MspA1I NcoI
NspI PciI PflMI PfoI PpuMI PsrI PstI SacI
SacII SexAI SfcI SmlI SspI StyI TaqII TatI
TstI
Enzymes that do not cut:
AarI AatII Acc65I AgeI AleI AlfI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BfrBI BglI BlpI BmtI BsaI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrFI BssHII BssSI
BstAPI BstZ17I BtgZI ClaI CspCI DraIII DrdI EagI
FseI FspI FspAI HindIII KasI KpnI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PvuI PvuII RsrII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI