Restriction map for
pF1KE1399
(Linear) (Six-base) MAPSORT of: KIEE1399.seq Check: 4646 from: 1 to: 5547
KIEE1399.orf
With 182 enzymes: SgfI *
July 25, 2014 11:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 956 2088 4594 5547
Size: 956 1132 2506 953
Fragments arranged by size:
2506 1132 956 953
AatII G_ACGT'C
Cuts at: 0 4823 5547
Size: 4823 724
AccI GT'mk_AC
Cuts at: 0 2747 2999 5547
Size: 2747 252 2548
Fragments arranged by size:
2747 2548 252
AclI AA'CG_TT
Cuts at: 0 4865 5547
Size: 4865 682
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 760 2635 2838 2909 5547
Size: 511 249 1875 203 71 2638
Fragments arranged by size:
2638 1875 511 249 203 71
AfeI AGC'GCT
Cuts at: 0 1287 3411 5547
Size: 1287 2124 2136
Fragments arranged by size:
2136 2124 1287
AflII C'TTAA_G
Cuts at: 0 2140 5547
Size: 2140 3407
AflIII A'CryG_T
Cuts at: 0 3911 5547
Size: 3911 1636
AleI CACnn'nnGTG
Cuts at: 0 169 1646 4105 5547
Size: 169 1477 2459 1442
Fragments arranged by size:
2459 1477 1442 169
AlwNI CAG_nnn'CTG
Cuts at: 0 939 1347 1856 1992 2598 4288 5538 5547
Size: 939 408 509 136 606 1690 1250 9
Fragments arranged by size:
1690 1250 939 606 509 408 136 9
ApaI G_GGCC'C
Cuts at: 0 1412 1553 3545 5547
Size: 1412 141 1992 2002
Fragments arranged by size:
2002 1992 1412 141
ApaLI G'TGCA_C
Cuts at: 0 3946 5547
Size: 3946 1601
ApoI r'AATT_y
Cuts at: 0 1954 2778 3519 4246 5547
Size: 1954 824 741 727 1301
Fragments arranged by size:
1954 1301 824 741 727
AvaI C'yCGr_G
Cuts at: 0 716 987 1241 1369 1405 1545 1579 2314
Size: 716 271 254 128 36 140 34 735
Cuts at: 2314 2519 3067 3379 3544 3592 3806 4093 4510
Size: 205 548 312 165 48 214 287 417
Cuts at: 4510 5492 5547
Size: 982 55
Fragments arranged by size:
982 735 716 548 417 312 287 271
254 214 205 165 140 128 55 48
36 34
BamHI G'GATC_C
Cuts at: 0 257 561 5547
Size: 257 304 4986
Fragments arranged by size:
4986 304 257
BanI G'GyrC_C
Cuts at: 0 136 1510 2217 3478 4041 5047 5547
Size: 136 1374 707 1261 563 1006 500
Fragments arranged by size:
1374 1261 1006 707 563 500 136
BanII G_rGCy'C
Cuts at: 0 666 1412 1553 1828 2276 2428 2494 3458
Size: 666 746 141 275 448 152 66 964
Cuts at: 3458 3545 4100 4138 5547
Size: 87 555 38 1409
Fragments arranged by size:
1409 964 746 666 555 448 275 152
141 87 66 38
BbeI G_GCGC'C
Cuts at: 0 3482 5547
Size: 3482 2065
BbsI GAAGACnn'nnnn_
Cuts at: 0 487 748 1390 1879 2897 3079 4550 5547
Size: 487 261 642 489 1018 182 1471 997
Fragments arranged by size:
1471 1018 997 642 489 487 261 182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3144 3177 3178 3211 3472 3506 4104 4138
Size: 3144 33 1 33 261 34 598 34
Cuts at: 4138 5547
Size: 1409
Fragments arranged by size:
3144 1409 598 261 34 34 33 33
1
BfrBI ATG'CAT
Cuts at: 0 212 500 5547
Size: 212 288 5047
Fragments arranged by size:
5047 288 212
BglI GCCn_nnn'nGGC
Cuts at: 0 1482 1584 3021 3718 5547
Size: 1482 102 1437 697 1829
Fragments arranged by size:
1829 1482 1437 697 102
BglII A'GATC_T
Cuts at: 0 4079 5547
Size: 4079 1468
BlpI GC'TnA_GC
Cuts at: 0 614 3413 5547
Size: 614 2799 2134
Fragments arranged by size:
2799 2134 614
Bme1580I G_kGCm'C
Cuts at: 0 929 978 1178 1406 1412 1553 1580 2220
Size: 929 49 200 228 6 141 27 640
Cuts at: 2220 2684 3545 3566 3950 4112 4767 5121 5504
Size: 464 861 21 384 162 655 354 383
Cuts at: 5504 5547
Size: 43
Fragments arranged by size:
929 861 655 640 464 384 383 354
228 200 162 141 49 43 27 21
6
BmgBI CAC'GTC
Cuts at: 0 3077 3527 3914 5116 5547
Size: 3077 450 387 1202 431
Fragments arranged by size:
3077 1202 450 431 387
BmrI ACTGGGnnnn_n'
Cuts at: 0 1305 4627 5547
Size: 1305 3322 920
Fragments arranged by size:
3322 1305 920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 961 2014 2287 4341 4774 5547
Size: 427 534 1053 273 2054 433 773
Fragments arranged by size:
2054 1053 773 534 433 427 273
Bpu10I CC'TnA_GC
Cuts at: 0 606 4643 5547
Size: 606 4037 904
Fragments arranged by size:
4037 904 606
BsaI GGTCTCn'nnnn_
Cuts at: 0 85 577 586 3841 3857 5139 5152 5547
Size: 85 492 9 3255 16 1282 13 395
Fragments arranged by size:
3255 1282 492 395 85 16 13 9
BsaAI yAC'GTr
Cuts at: 0 930 1401 5547
Size: 930 471 4146
Fragments arranged by size:
4146 930 471
BsaBI GATnn'nnATC
Cuts at: 0 3901 5547
Size: 3901 1646
BsaHI Gr'CG_yC
Cuts at: 0 2473 3479 3959 4451 4820 5547
Size: 2473 1006 480 492 369 727
Fragments arranged by size:
2473 1006 727 492 480 369
BsaWI w'CCGG_w
Cuts at: 0 586 2294 3866 5547
Size: 586 1708 1572 1681
Fragments arranged by size:
1708 1681 1572 586
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1735 1765 2860 2890 3826 3856 4562 4592
Size: 1735 30 1095 30 936 30 706 30
Cuts at: 4592 5178 5208 5547
Size: 586 30 339
Fragments arranged by size:
1735 1095 936 706 586 339 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 367 1756 1882 2284 2332 2594 3397
Size: 163 204 1389 126 402 48 262 803
Cuts at: 3397 4166 4226 4489 5547
Size: 769 60 263 1058
Fragments arranged by size:
1389 1058 803 769 402 263 262 204
163 126 60 48
BseYI C'CCAG_C
Cuts at: 0 1340 1360 1440 1977 2401 3505 3555 3634
Size: 1340 20 80 537 424 1104 50 79
Cuts at: 3634 3763 3917 4766 5054 5268 5547
Size: 129 154 849 288 214 279
Fragments arranged by size:
1340 1104 849 537 424 288 279 214
154 129 80 79 50 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 952 4572 5547
Size: 838 114 3620 975
Fragments arranged by size:
3620 975 838 114
BsiEI CG_ry'CG
Cuts at: 0 3264 5547
Size: 3264 2283
BsiHKAI G_wGCw'C
Cuts at: 0 666 947 2276 3082 3495 3950 4999 5020
Size: 666 281 1329 806 413 455 1049 21
Cuts at: 5020 5547
Size: 527
Fragments arranged by size:
1329 1049 806 666 527 455 413 281
21
BsmI GAATG_Cn'
Cuts at: 0 212 987 2913 5547
Size: 212 775 1926 2634
Fragments arranged by size:
2634 1926 775 212
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3823 4507 5547
Size: 3823 684 1040
Fragments arranged by size:
3823 1040 684
Bsp1286I G_dGCh'C
Cuts at: 0 666 929 947 978 1178 1406 1412 1553
Size: 666 263 18 31 200 228 6 141
Cuts at: 1553 1580 1828 2220 2276 2428 2494 2684 3082
Size: 27 248 392 56 152 66 190 398
Cuts at: 3082 3458 3495 3545 3566 3950 4100 4112 4138
Size: 376 37 50 21 384 150 12 26
Cuts at: 4138 4767 4999 5020 5121 5504 5547
Size: 629 232 21 101 383 43
Fragments arranged by size:
666 629 398 392 384 383 376 263
248 232 228 200 190 152 150 141
101 66 56 50 43 37 31 27
26 21 21 18 12 6
BspEI T'CCGG_A
Cuts at: 0 2294 5547
Size: 2294 3253
BspHI T'CATG_A
Cuts at: 0 4991 5547
Size: 4991 556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 203 908 956 2088 4594 5465 5547
Size: 203 705 48 1132 2506 871 82
Fragments arranged by size:
2506 1132 871 705 203 82 48
BsrBI CCG'CTC
Cuts at: 0 357 613 2589 4741 5206 5547
Size: 357 256 1976 2152 465 341
Fragments arranged by size:
2152 1976 465 357 341 256
BsrDI GCAATG_nn'
Cuts at: 0 330 4228 4683 5547
Size: 330 3898 455 864
Fragments arranged by size:
3898 864 455 330
BsrFI r'CCGG_y
Cuts at: 0 315 1208 1418 1968 2408 2911 3586 3775
Size: 315 893 210 550 440 503 675 189
Cuts at: 3775 5075 5547
Size: 1300 472
Fragments arranged by size:
1300 893 675 550 503 472 440 315
210 189
BsrGI T'GTAC_A
Cuts at: 0 265 5547
Size: 265 5282
BssSI C'ACGA_G
Cuts at: 0 898 2275 4699 5547
Size: 898 1377 2424 848
Fragments arranged by size:
2424 1377 898 848
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1356 2411 2679 5547
Size: 1356 1055 268 2868
Fragments arranged by size:
2868 1356 1055 268
BstEII G'GTnAC_C
Cuts at: 0 2154 3789 5025 5547
Size: 2154 1635 1236 522
Fragments arranged by size:
2154 1635 1236 522
BstXI CCAn_nnnn'nTGG
Cuts at: 0 546 1230 2166 3318 3969 5547
Size: 546 684 936 1152 651 1578
Fragments arranged by size:
1578 1152 936 684 651 546
BstYI r'GATC_y
Cuts at: 0 257 561 902 1631 4079 5250 5547
Size: 257 304 341 729 2448 1171 297
Fragments arranged by size:
2448 1171 729 341 304 297 257
Bsu36I CC'TnA_GG
Cuts at: 0 3817 5103 5547
Size: 3817 1286 444
Fragments arranged by size:
3817 1286 444
BtgI C'CryG_G
Cuts at: 0 59 383 1310 1644 1704 1820 2000 2819
Size: 59 324 927 334 60 116 180 819
Cuts at: 2819 3953 4139 4193 5547
Size: 1134 186 54 1354
Fragments arranged by size:
1354 1134 927 819 334 324 186 180
116 60 59 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 502 1412 3228 3890 5547
Size: 502 910 1816 662 1657
Fragments arranged by size:
1816 1657 910 662 502
BtsI GCAGTG_nn'
Cuts at: 0 116 3566 4767 5547
Size: 116 3450 1201 780
Fragments arranged by size:
3450 1201 780 116
ClaI AT'CG_AT
Cuts at: 0 5435 5477 5547
Size: 5435 42 70
Fragments arranged by size:
5435 70 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 639 674 5111 5146 5547
Size: 639 35 4437 35 401
Fragments arranged by size:
4437 639 401 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 239 872 3954 5547
Size: 239 633 3082 1593
Fragments arranged by size:
3082 1593 633 239
DrdI GACnn_nn'nnGTC
Cuts at: 0 1300 5547
Size: 1300 4247
EaeI y'GGCC_r
Cuts at: 0 317 464 1109 1232 1313 1707 2088 2822
Size: 317 147 645 123 81 394 381 734
Cuts at: 2822 4778 5372 5547
Size: 1956 594 175
Fragments arranged by size:
1956 734 645 594 394 381 317 175
147 123 81
EarI CTCTTCn'nnn_
Cuts at: 0 83 1594 2579 3983 4139 5547
Size: 83 1511 985 1404 156 1408
Fragments arranged by size:
1511 1408 1404 985 156 83
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1076 1744 3039 4950 5547
Size: 1076 668 1295 1911 597
Fragments arranged by size:
1911 1295 1076 668 597
EcoICRI GAG'CTC
Cuts at: 0 664 2274 5547
Size: 664 1610 3273
Fragments arranged by size:
3273 1610 664
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 511 760 961 2014 2287 2635 2838
Size: 427 84 249 201 1053 273 348 203
Cuts at: 2838 2909 4341 4774 5547
Size: 71 1432 433 773
Fragments arranged by size:
1432 1053 773 433 427 348 273 249
203 201 84 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 349 5461 5547
Size: 349 5112 86
Fragments arranged by size:
5112 349 86
EcoO109I rG'GnC_Cy
Cuts at: 0 1188 1245 1409 1549 3174 3541 3697 3711
Size: 1188 57 164 140 1625 367 156 14
Cuts at: 3711 3811 4799 5099 5469 5547
Size: 100 988 300 370 78
Fragments arranged by size:
1625 1188 988 370 367 300 164 156
140 100 78 57 14
EcoRI G'AATT_C
Cuts at: 0 1954 2778 3519 5547
Size: 1954 824 741 2028
Fragments arranged by size:
2028 1954 824 741
EcoRV GAT'ATC
Cuts at: 0 4266 5547
Size: 4266 1281
HaeII r_GCGC'y
Cuts at: 0 1289 1703 3413 3482 5547
Size: 1289 414 1710 69 2065
Fragments arranged by size:
2065 1710 1289 414 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1513 1545 2148 2180 2272 2304 2422 2454
Size: 1513 32 603 32 92 32 118 32
Cuts at: 2454 2860 2892 3406 3438 3622 3654 5547
Size: 406 32 514 32 184 32 1893
Fragments arranged by size:
1893 1513 603 514 406 184 118 92
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2064 4623 5547
Size: 2064 2559 924
Fragments arranged by size:
2559 2064 924
HindIII A'AGCT_T
Cuts at: 0 1801 4443 5547
Size: 1801 2642 1104
Fragments arranged by size:
2642 1801 1104
HpaI GTT'AAC
Cuts at: 0 2064 5547
Size: 2064 3483
KasI G'GCGC_C
Cuts at: 0 3478 5547
Size: 3478 2069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 74 801 1226 1283 2837 2884 3074
Size: 47 27 727 425 57 1554 47 190
Cuts at: 3074 5547
Size: 2473
Fragments arranged by size:
2473 1554 727 425 190 57 47 47
27
MscI TGG'CCA
Cuts at: 0 466 1111 2824 4780 5547
Size: 466 645 1713 1956 767
Fragments arranged by size:
1956 1713 767 645 466
MslI CAynn'nnrTG
Cuts at: 0 169 541 1646 2365 2830 3103 4105 5547
Size: 169 372 1105 719 465 273 1002 1442
Fragments arranged by size:
1442 1105 1002 719 465 372 273 169
MspA1I CmG'CkG
Cuts at: 0 255 618 801 1034 1344 1646 1706 2397
Size: 255 363 183 233 310 302 60 691
Cuts at: 2397 3131 3195 3420 3516 3578 3645 4087 5272
Size: 734 64 225 96 62 67 442 1185
Cuts at: 5272 5535 5547
Size: 263 12
Fragments arranged by size:
1185 734 691 442 363 310 302 263
255 233 225 183 96 67 64 62
60 12
NaeI GCC'GGC
Cuts at: 0 317 1210 2913 3588 3777 5077 5547
Size: 317 893 1703 675 189 1300 470
Fragments arranged by size:
1703 1300 893 675 470 317 189
NarI GG'CG_CC
Cuts at: 0 3479 5547
Size: 3479 2068
NcoI C'CATG_G
Cuts at: 0 4139 5547
Size: 4139 1408
NgoMIV G'CCGG_C
Cuts at: 0 315 1208 2911 3586 3775 5075 5547
Size: 315 893 1703 675 189 1300 472
Fragments arranged by size:
1703 1300 893 675 472 315 189
NsiI A_TGCA'T
Cuts at: 0 214 502 5547
Size: 214 288 5045
Fragments arranged by size:
5045 288 214
NspI r_CATG'y
Cuts at: 0 1489 3894 5547
Size: 1489 2405 1653
Fragments arranged by size:
2405 1653 1489
PasI CC'CwG_GG
Cuts at: 0 1760 3449 3537 5547
Size: 1760 1689 88 2010
Fragments arranged by size:
2010 1760 1689 88
PflMI CCAn_nnn'nTGG
Cuts at: 0 239 3471 4811 5547
Size: 239 3232 1340 736
Fragments arranged by size:
3232 1340 736 239
PfoI T'CCnGG_A
Cuts at: 0 344 5547
Size: 344 5203
PmlI CAC'GTG
Cuts at: 0 930 5547
Size: 930 4617
PpuMI rG'GwC_Cy
Cuts at: 0 1245 3697 3811 4799 5099 5469 5547
Size: 1245 2452 114 988 300 370 78
Fragments arranged by size:
2452 1245 988 370 300 114 78
PshAI GACnn'nnGTC
Cuts at: 0 1879 5547
Size: 1879 3668
PsiI TTA'TAA
Cuts at: 0 776 5547
Size: 776 4771
PspOMI G'GGCC_C
Cuts at: 0 1408 1549 3541 5547
Size: 1408 141 1992 2006
Fragments arranged by size:
2006 1992 1408 141
PspXI vC'TCGA_Gb
Cuts at: 0 2519 5492 5547
Size: 2519 2973 55
Fragments arranged by size:
2973 2519 55
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2428 2460 5547
Size: 2428 32 3087
Fragments arranged by size:
3087 2428 32
PstI C_TGCA'G
Cuts at: 0 200 919 1352 1889 3986 4380 5072 5462
Size: 200 719 433 537 2097 394 692 390
Cuts at: 5462 5547
Size: 85
Fragments arranged by size:
2097 719 692 537 433 394 390 200
85
PvuII CAG'CTG
Cuts at: 0 618 1034 1344 3195 3420 5535 5547
Size: 618 416 310 1851 225 2115 12
Fragments arranged by size:
2115 1851 618 416 310 225 12
RsrII CG'GwC_CG
Cuts at: 0 2292 5547
Size: 2292 3255
SacI G_AGCT'C
Cuts at: 0 666 2276 5547
Size: 666 1610 3271
Fragments arranged by size:
3271 1610 666
SacII CC_GC'GG
Cuts at: 0 1647 1707 5547
Size: 1647 60 3840
Fragments arranged by size:
3840 1647 60
SanDI GG'GwC_CC
Cuts at: 0 3697 3811 5547
Size: 3697 114 1736
Fragments arranged by size:
3697 1736 114
SapI GCTCTTCn'nnn_
Cuts at: 0 1594 2579 5547
Size: 1594 985 2968
Fragments arranged by size:
2968 1594 985
SbfI CC_TGCA'GG
Cuts at: 0 919 3986 5547
Size: 919 3067 1561
Fragments arranged by size:
3067 1561 919
SexAI A'CCwGG_T
Cuts at: 0 2132 2806 5547
Size: 2132 674 2741
Fragments arranged by size:
2741 2132 674
SfcI C'TryA_G
Cuts at: 0 196 915 1348 1566 1885 1984 2898 3982
Size: 196 719 433 218 319 99 914 1084
Cuts at: 3982 4376 4891 5068 5458 5547
Size: 394 515 177 390 89
Fragments arranged by size:
1084 914 719 515 433 394 390 319
218 196 177 99 89
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3718 5547
Size: 3718 1829
SfoI GGC'GCC
Cuts at: 0 3480 5547
Size: 3480 2067
SgrAI Cr'CCGG_yG
Cuts at: 0 1208 5547
Size: 1208 4339
SmaI CCC'GGG
Cuts at: 0 1243 1407 1547 1581 3594 3808 5547
Size: 1243 164 140 34 2013 214 1739
Fragments arranged by size:
2013 1739 1243 214 164 140 34
SmlI C'TyrA_G
Cuts at: 0 2140 2519 4510 5492 5547
Size: 2140 379 1991 982 55
Fragments arranged by size:
2140 1991 982 379 55
SrfI GCCC'GGGC
Cuts at: 0 1407 5547
Size: 1407 4140
StuI AGG'CCT
Cuts at: 0 557 913 5416 5547
Size: 557 356 4503 131
Fragments arranged by size:
4503 557 356 131
StyI C'CwwG_G
Cuts at: 0 2454 2789 2879 3716 4139 4781 4795 5547
Size: 2454 335 90 837 423 642 14 752
Fragments arranged by size:
2454 837 752 642 423 335 90 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 376 566 1959 2280 3463 3623 3686 3865
Size: 376 190 1393 321 1183 160 63 179
Cuts at: 3865 5389 5547
Size: 1524 158
Fragments arranged by size:
1524 1393 1183 376 321 190 179 160
158 63
TatI w'GTAC_w
Cuts at: 0 265 2849 5547
Size: 265 2584 2698
Fragments arranged by size:
2698 2584 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1735 1767 1958 1990 2450 2482 4562 4594
Size: 1735 32 191 32 460 32 2080 32
Cuts at: 4594 5178 5210 5547
Size: 584 32 337
Fragments arranged by size:
2080 1735 584 460 337 191 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1654 4453 5547
Size: 1654 2799 1094
Fragments arranged by size:
2799 1654 1094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3472 3642 4128 5242 5547
Size: 3472 170 486 1114 305
Fragments arranged by size:
3472 1114 486 305 170
XhoI C'TCGA_G
Cuts at: 0 2519 4510 5492 5547
Size: 2519 1991 982 55
Fragments arranged by size:
2519 1991 982 55
XmaI C'CCGG_G
Cuts at: 0 1241 1405 1545 1579 3592 3806 5547
Size: 1241 164 140 34 2013 214 1741
Fragments arranged by size:
2013 1741 1241 214 164 140 34
XmnI GAAnn'nnTTC
Cuts at: 0 1804 5547
Size: 1804 3743
ZraI GAC'GTC
Cuts at: 0 4821 5547
Size: 4821 726
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AleI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BcgI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
HaeII Hin4I HincII HindIII HpaI KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuII RsrII SacI SacII SanDI
SapI SbfI SexAI SfcI SfiI SfoI SgrAI SmaI
SmlI SrfI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AgeI AhdI AlfI AloI AscI AseI AsiSI
AvrII BaeI BbvCI BciVI BclI BmtI BplI BpuEI
BsiWI BssHII BstBI BstZ17I DraI EagI FalI FseI
FspI FspAI KpnI MfeI MluI NdeI NheI NotI
NruI PacI PciI PmeI PpiI PvuI SalI ScaI
SgfI SnaBI SpeI SphI SspI SwaI XbaI