Restriction map for
pF1KE1429
(Linear) (Six-base) MAPSORT of: KIEE1429.seq Check: 4780 from: 1 to: 1608
KIEE1429.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AccI GT'mk_AC
Cuts at: 0 209 1608
Size: 209 1399
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 187 1608
Size: 88 99 1421
Fragments arranged by size:
1421 99 88
AflIII A'CryG_T
Cuts at: 0 1004 1608
Size: 1004 604
AhdI GACnn_n'nnGTC
Cuts at: 0 1356 1536 1608
Size: 1356 180 72
Fragments arranged by size:
1356 180 72
AleI CACnn'nnGTG
Cuts at: 0 433 1246 1608
Size: 433 813 362
Fragments arranged by size:
813 433 362
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 397 398 429 430 1608
Size: 397 1 31 1 1178
Fragments arranged by size:
1178 397 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 261 1338 1608
Size: 261 1077 270
Fragments arranged by size:
1077 270 261
ApaI G_GGCC'C
Cuts at: 0 442 1608
Size: 442 1166
ApaLI G'TGCA_C
Cuts at: 0 376 733 1608
Size: 376 357 875
Fragments arranged by size:
875 376 357
ApoI r'AATT_y
Cuts at: 0 1171 1608
Size: 1171 437
AvaI C'yCGr_G
Cuts at: 0 326 1608
Size: 326 1282
BanI G'GyrC_C
Cuts at: 0 1385 1608
Size: 1385 223
BanII G_rGCy'C
Cuts at: 0 442 758 975 1292 1608
Size: 442 316 217 317 316
Fragments arranged by size:
442 317 316 316 217
BbeI G_GCGC'C
Cuts at: 0 1389 1608
Size: 1389 219
BbsI GAAGACnn'nnnn_
Cuts at: 0 1036 1045 1608
Size: 1036 9 563
Fragments arranged by size:
1036 563 9
Bme1580I G_kGCm'C
Cuts at: 0 380 442 737 808 1608
Size: 380 62 295 71 800
Fragments arranged by size:
800 380 295 71 62
BmgBI CAC'GTC
Cuts at: 0 1314 1608
Size: 1314 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 82 199 895 1078 1608
Size: 55 27 117 696 183 530
Fragments arranged by size:
696 530 183 117 55 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1608
Size: 457 1151
BsaI GGTCTCn'nnnn_
Cuts at: 0 113 1409 1608
Size: 113 1296 199
Fragments arranged by size:
1296 199 113
BsaHI Gr'CG_yC
Cuts at: 0 1386 1608
Size: 1386 222
BsaWI w'CCGG_w
Cuts at: 0 24 416 1608
Size: 24 392 1192
Fragments arranged by size:
1192 392 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 250 280 1186 1216 1608
Size: 55 30 165 30 906 30 392
Fragments arranged by size:
906 392 165 55 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 175 364 1608
Size: 52 123 189 1244
Fragments arranged by size:
1244 189 123 52
BseYI C'CCAG_C
Cuts at: 0 79 317 391 1372 1608
Size: 79 238 74 981 236
Fragments arranged by size:
981 238 236 79 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 1608
Size: 406 1202
BsiHKAI G_wGCw'C
Cuts at: 0 380 737 758 800 1608
Size: 380 357 21 42 808
Fragments arranged by size:
808 380 357 42 21
BsmI GAATG_Cn'
Cuts at: 0 1463 1608
Size: 1463 145
Bsp1286I G_dGCh'C
Cuts at: 0 380 442 737 758 800 808 975 1292
Size: 380 62 295 21 42 8 167 317
Cuts at: 1292 1608
Size: 316
Fragments arranged by size:
380 317 316 295 167 62 42 21
8
BspEI T'CCGG_A
Cuts at: 0 24 416 1608
Size: 24 392 1192
Fragments arranged by size:
1192 392 24
BsrDI GCAATG_nn'
Cuts at: 0 705 1608
Size: 705 903
BsrGI T'GTAC_A
Cuts at: 0 854 1608
Size: 854 754
BstAPI GCAn_nnn'nTGC
Cuts at: 0 261 384 1608
Size: 261 123 1224
Fragments arranged by size:
1224 261 123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1248 1608
Size: 1248 360
BstYI r'GATC_y
Cuts at: 0 395 483 674 1608
Size: 395 88 191 934
Fragments arranged by size:
934 395 191 88
Bsu36I CC'TnA_GG
Cuts at: 0 538 1608
Size: 538 1070
BtsI GCAGTG_nn'
Cuts at: 0 735 1135 1608
Size: 735 400 473
Fragments arranged by size:
735 473 400
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1129 1164 1608
Size: 1129 35 444
Fragments arranged by size:
1129 444 35
DraIII CAC_nnn'GTG
Cuts at: 0 384 1228 1608
Size: 384 844 380
Fragments arranged by size:
844 384 380
EaeI y'GGCC_r
Cuts at: 0 761 995 1608
Size: 761 234 613
Fragments arranged by size:
761 613 234
EarI CTCTTCn'nnn_
Cuts at: 0 1318 1547 1608
Size: 1318 229 61
Fragments arranged by size:
1318 229 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1357 1608
Size: 1357 251
EcoICRI GAG'CTC
Cuts at: 0 756 1608
Size: 756 852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 82 88 187 199 895 1078 1608
Size: 55 27 6 99 12 696 183 530
Fragments arranged by size:
696 530 183 99 55 27 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 946 1165 1608
Size: 946 219 443
Fragments arranged by size:
946 443 219
EcoO109I rG'GnC_Cy
Cuts at: 0 439 1080 1158 1557 1608
Size: 439 641 78 399 51
Fragments arranged by size:
641 439 399 78 51
EcoRI G'AATT_C
Cuts at: 0 1171 1608
Size: 1171 437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1062 1608
Size: 1062 546
HaeII r_GCGC'y
Cuts at: 0 1389 1608
Size: 1389 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 55 69 87 250 282 962 994
Size: 37 18 14 18 163 32 680 32
Cuts at: 994 1336 1342 1368 1374 1522 1554 1608
Size: 342 6 26 6 148 32 54
Fragments arranged by size:
680 342 163 148 54 37 32 32
32 26 18 18 14 6 6
HindIII A'AGCT_T
Cuts at: 0 100 1608
Size: 100 1508
KasI G'GCGC_C
Cuts at: 0 1385 1608
Size: 1385 223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 116 725 1129 1608
Size: 23 93 609 404 479
Fragments arranged by size:
609 479 404 93 23
MscI TGG'CCA
Cuts at: 0 763 997 1608
Size: 763 234 611
Fragments arranged by size:
763 611 234
MslI CAynn'nnrTG
Cuts at: 0 433 727 791 985 1003 1246 1608
Size: 433 294 64 194 18 243 362
Fragments arranged by size:
433 362 294 243 194 64 18
MspA1I CmG'CkG
Cuts at: 0 1056 1608
Size: 1056 552
NarI GG'CG_CC
Cuts at: 0 1386 1608
Size: 1386 222
NspI r_CATG'y
Cuts at: 0 1008 1608
Size: 1008 600
PciI A'CATG_T
Cuts at: 0 1004 1608
Size: 1004 604
PflMI CCAn_nnn'nTGG
Cuts at: 0 1130 1608
Size: 1130 478
PfoI T'CCnGG_A
Cuts at: 0 620 1608
Size: 620 988
PspOMI G'GGCC_C
Cuts at: 0 438 1608
Size: 438 1170
PstI C_TGCA'G
Cuts at: 0 257 780 1608
Size: 257 523 828
Fragments arranged by size:
828 523 257
PvuII CAG'CTG
Cuts at: 0 1056 1608
Size: 1056 552
SacI G_AGCT'C
Cuts at: 0 758 1608
Size: 758 850
SfcI C'TryA_G
Cuts at: 0 253 776 1608
Size: 253 523 832
Fragments arranged by size:
832 523 253
SfoI GGC'GCC
Cuts at: 0 1387 1608
Size: 1387 221
SmaI CCC'GGG
Cuts at: 0 328 1608
Size: 328 1280
SmlI C'TyrA_G
Cuts at: 0 472 1608
Size: 472 1136
SrfI GCCC'GGGC
Cuts at: 0 328 1608
Size: 328 1280
StyI C'CwwG_G
Cuts at: 0 629 947 1076 1154 1608
Size: 629 318 129 78 454
Fragments arranged by size:
629 454 318 129 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 95 331 1133 1608
Size: 95 236 802 475
Fragments arranged by size:
802 475 236 95
TatI w'GTAC_w
Cuts at: 0 182 650 854 1608
Size: 182 468 204 754
Fragments arranged by size:
754 468 204 182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 475 507 1608
Size: 475 32 1101
Fragments arranged by size:
1101 475 32
Tth111I GACn'n_nGTC
Cuts at: 0 834 1608
Size: 834 774
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1127 1131 1161 1428 1608
Size: 1127 4 30 267 180
Fragments arranged by size:
1127 267 180 30 4
XmaI C'CCGG_G
Cuts at: 0 326 1608
Size: 326 1282
XmnI GAAnn'nnTTC
Cuts at: 0 931 1608
Size: 931 677
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI Bme1580I
BmgBI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrDI BsrGI
BstAPI BstXI BstYI Bsu36I BtsI CspCI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I
NarI NspI PciI PflMI PfoI PspOMI PstI PvuII
SacI SfcI SfoI SmaI SmlI SrfI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AlfI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI
BplI Bpu10I BsaAI BsaBI BsiEI BsiWI BsmBI BspHI
BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I
BtgI BtgZI ClaI DraI DrdI EagI EcoRV FseI
FspI FspAI HincII HpaI KpnI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SspI StuI SwaI XbaI XhoI ZraI