Restriction map for
pF1KE1498
(Linear) (Six-base) MAPSORT of: KIEE1498.seq Check: 14 from: 1 to: 4773
KIEE1498.orf
With 182 enzymes: SgfI *
July 15, 2010 17:11 ..
AccI GT'mk_AC
Cuts at: 0 335 1932 4773
Size: 335 1597 2841
Fragments arranged by size:
2841 1597 335
Acc65I G'GTAC_C
Cuts at: 0 238 3324 4773
Size: 238 3086 1449
Fragments arranged by size:
3086 1449 238
AclI AA'CG_TT
Cuts at: 0 2243 4773
Size: 2243 2530
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 851 914 1751 1867 3969 4115 4294 4567
Size: 851 63 837 116 2102 146 179 273
Cuts at: 4567 4773
Size: 206
Fragments arranged by size:
2102 851 837 273 206 179 146 116
63
AfeI AGC'GCT
Cuts at: 0 4016 4773
Size: 4016 757
AflIII A'CryG_T
Cuts at: 0 21 4637 4773
Size: 21 4616 136
Fragments arranged by size:
4616 136 21
AgeI A'CCGG_T
Cuts at: 0 2641 4773
Size: 2641 2132
AhdI GACnn_n'nnGTC
Cuts at: 0 1035 1443 4773
Size: 1035 408 3330
Fragments arranged by size:
3330 1035 408
AleI CACnn'nnGTG
Cuts at: 0 3163 3814 4773
Size: 3163 651 959
Fragments arranged by size:
3163 959 651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 949 4773
Size: 949 3824
AlwNI CAG_nnn'CTG
Cuts at: 0 201 364 385 674 826 1774 2563 4309
Size: 201 163 21 289 152 948 789 1746
Cuts at: 4309 4773
Size: 464
Fragments arranged by size:
1746 948 789 464 289 201 163 152
21
ApaI G_GGCC'C
Cuts at: 0 470 4773
Size: 470 4303
ApaLI G'TGCA_C
Cuts at: 0 1339 4268 4773
Size: 1339 2929 505
Fragments arranged by size:
2929 1339 505
ApoI r'AATT_y
Cuts at: 0 544 754 774 2428 3258 3289 4773
Size: 544 210 20 1654 830 31 1484
Fragments arranged by size:
1654 1484 830 544 210 31 20
AvaI C'yCGr_G
Cuts at: 0 129 632 1831 2470 2782 4773
Size: 129 503 1199 639 312 1991
Fragments arranged by size:
1991 1199 639 503 312 129
BamHI G'GATC_C
Cuts at: 0 3575 4773
Size: 3575 1198
BanI G'GyrC_C
Cuts at: 0 238 898 1243 1312 1512 1655 3324 4549
Size: 238 660 345 69 200 143 1669 1225
Cuts at: 4549 4773
Size: 224
Fragments arranged by size:
1669 1225 660 345 238 224 200 143
69
BanII G_rGCy'C
Cuts at: 0 224 262 470 1255 2860 3055 3143 4773
Size: 224 38 208 785 1605 195 88 1630
Fragments arranged by size:
1630 1605 785 224 208 195 88 38
BbeI G_GCGC'C
Cuts at: 0 1316 4773
Size: 1316 3457
BbsI GAAGACnn'nnnn_
Cuts at: 0 254 1533 1676 1757 2034 2483 3876 4203
Size: 254 1279 143 81 277 449 1393 327
Cuts at: 4203 4742 4773
Size: 539 31
Fragments arranged by size:
1393 1279 539 449 327 277 254 143
81 31
BbvCI CC'TCA_GC
Cuts at: 0 428 1549 3080 4773
Size: 428 1121 1531 1693
Fragments arranged by size:
1693 1531 1121 428
BciVI GTATCCnnnnn_n'
Cuts at: 0 2091 4773
Size: 2091 2682
BclI T'GATC_A
Cuts at: 0 2976 3354 4773
Size: 2976 378 1419
Fragments arranged by size:
2976 1419 378
BglI GCCn_nnn'nGGC
Cuts at: 0 1216 1996 4288 4773
Size: 1216 780 2292 485
Fragments arranged by size:
2292 1216 780 485
BglII A'GATC_T
Cuts at: 0 1808 3780 4773
Size: 1808 1972 993
Fragments arranged by size:
1972 1808 993
BlpI GC'TnA_GC
Cuts at: 0 667 4773
Size: 667 4106
Bme1580I G_kGCm'C
Cuts at: 0 470 1297 1343 2891 4272 4773
Size: 470 827 46 1548 1381 501
Fragments arranged by size:
1548 1381 827 501 470 46
BmgBI CAC'GTC
Cuts at: 0 3246 3654 4773
Size: 3246 408 1119
Fragments arranged by size:
3246 1119 408
BmrI ACTGGGnnnn_n'
Cuts at: 0 189 1404 3984 4773
Size: 189 1215 2580 789
Fragments arranged by size:
2580 1215 789 189
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 243 1230 3074 4773
Size: 243 987 1844 1699
Fragments arranged by size:
1844 1699 987 243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 743 1047 1192 1222 2039 2392 2809
Size: 238 505 304 145 30 817 353 417
Cuts at: 2809 2915 3166 3565 4717 4734 4773
Size: 106 251 399 1152 17 39
Fragments arranged by size:
1152 817 505 417 399 353 304 251
238 145 106 39 30 17
Bpu10I CC'TnA_GC
Cuts at: 0 428 1249 1517 1549 3080 3598 4773
Size: 428 821 268 32 1531 518 1175
Fragments arranged by size:
1531 1175 821 518 428 268 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 2707 3364 3565 4642 4773
Size: 312 2395 657 201 1077 131
Fragments arranged by size:
2395 1077 657 312 201 131
BsaI GGTCTCn'nnnn_
Cuts at: 0 288 2917 2932 3788 4559 4773
Size: 288 2629 15 856 771 214
Fragments arranged by size:
2629 856 771 288 214 15
BsaAI yAC'GTr
Cuts at: 0 2622 3165 4773
Size: 2622 543 1608
Fragments arranged by size:
2622 1608 543
BsaHI Gr'CG_yC
Cuts at: 0 1313 2711 4773
Size: 1313 1398 2062
Fragments arranged by size:
2062 1398 1313
BsaWI w'CCGG_w
Cuts at: 0 2641 4773
Size: 2641 2132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 136 166 4393 4423 4773
Size: 136 30 4227 30 350
Fragments arranged by size:
4227 350 136 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 403 640 830 1219 2950 3050 3649 4195
Size: 403 237 190 389 1731 100 599 546
Cuts at: 4195 4347 4350 4468 4495 4773
Size: 152 3 118 27 278
Fragments arranged by size:
1731 599 546 403 389 278 237 190
152 118 100 27 3
BseYI C'CCAG_C
Cuts at: 0 184 328 2757 3843 4232 4412 4773
Size: 184 144 2429 1086 389 180 361
Fragments arranged by size:
2429 1086 389 361 184 180 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1525 2342 4061 4773
Size: 388 1137 817 1719 712
Fragments arranged by size:
1719 1137 817 712 388
BsiEI CG_ry'CG
Cuts at: 0 1649 2339 4773
Size: 1649 690 2434
Fragments arranged by size:
2434 1649 690
BsiHKAI G_wGCw'C
Cuts at: 0 32 166 1183 1255 1343 1523 3143 3641
Size: 32 134 1017 72 88 180 1620 498
Cuts at: 3641 4272 4773
Size: 631 501
Fragments arranged by size:
1620 1017 631 501 498 180 134 88
72 32
BsmI GAATG_Cn'
Cuts at: 0 447 3499 3877 4170 4773
Size: 447 3052 378 293 603
Fragments arranged by size:
3052 603 447 378 293
BsmBI CGTCTCn'nnnn_
Cuts at: 0 475 3142 4389 4773
Size: 475 2667 1247 384
Fragments arranged by size:
2667 1247 475 384
Bsp1286I G_dGCh'C
Cuts at: 0 32 166 224 262 470 1183 1255 1297
Size: 32 134 58 38 208 713 72 42
Cuts at: 1297 1343 1523 2860 2891 3055 3143 3641 4272
Size: 46 180 1337 31 164 88 498 631
Cuts at: 4272 4773
Size: 501
Fragments arranged by size:
1337 713 631 501 498 208 180 164
134 88 72 58 46 42 38 32
31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1433 2063 2426 3806 4011 4577 4773
Size: 1433 630 363 1380 205 566 196
Fragments arranged by size:
1433 1380 630 566 363 205 196
BsrBI CCG'CTC
Cuts at: 0 83 2929 4578 4773
Size: 83 2846 1649 195
Fragments arranged by size:
2846 1649 195 83
BsrFI r'CCGG_y
Cuts at: 0 1210 1309 2641 3834 4287 4773
Size: 1210 99 1332 1193 453 486
Fragments arranged by size:
1332 1210 1193 486 453 99
BsrGI T'GTAC_A
Cuts at: 0 2495 4773
Size: 2495 2278
BssSI C'ACGA_G
Cuts at: 0 31 2281 4773
Size: 31 2250 2492
Fragments arranged by size:
2492 2250 31
BstAPI GCAn_nnn'nTGC
Cuts at: 0 375 1512 4309 4773
Size: 375 1137 2797 464
Fragments arranged by size:
2797 1137 464 375
BstBI TT'CG_AA
Cuts at: 0 2610 4773
Size: 2610 2163
BstXI CCAn_nnnn'nTGG
Cuts at: 0 270 477 693 3465 4773
Size: 270 207 216 2772 1308
Fragments arranged by size:
2772 1308 270 216 207
BstYI r'GATC_y
Cuts at: 0 1808 2050 3575 3780 3946 4773
Size: 1808 242 1525 205 166 827
Fragments arranged by size:
1808 1525 827 242 205 166
Bsu36I CC'TnA_GG
Cuts at: 0 3062 4773
Size: 3062 1711
BtgI C'CryG_G
Cuts at: 0 658 1949 3071 4007 4773
Size: 658 1291 1122 936 766
Fragments arranged by size:
1291 1122 936 766 658
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1241 4773
Size: 1241 3532
BtsI GCAGTG_nn'
Cuts at: 0 990 1640 2354 2922 4310 4773
Size: 990 650 714 568 1388 463
Fragments arranged by size:
1388 990 714 650 568 463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1925 1960 4773
Size: 1925 35 2813
Fragments arranged by size:
2813 1925 35
DraI TTT'AAA
Cuts at: 0 3176 4773
Size: 3176 1597
DraIII CAC_nnn'GTG
Cuts at: 0 2829 4773
Size: 2829 1944
DrdI GACnn_nn'nnGTC
Cuts at: 0 319 2275 4773
Size: 319 1956 2498
Fragments arranged by size:
2498 1956 319
EaeI y'GGCC_r
Cuts at: 0 1319 1946 2125 2344 3005 3656 4289 4773
Size: 1319 627 179 219 661 651 633 484
Fragments arranged by size:
1319 661 651 633 627 484 219 179
EarI CTCTTCn'nnn_
Cuts at: 0 321 605 827 939 2727 3212 3710 4773
Size: 321 284 222 112 1788 485 498 1063
Fragments arranged by size:
1788 1063 498 485 321 284 222 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 594 4773
Size: 594 4179
EcoICRI GAG'CTC
Cuts at: 0 1253 3141 4773
Size: 1253 1888 1632
Fragments arranged by size:
1888 1632 1253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 743 851 914 1047 1192 1222 1751
Size: 238 505 108 63 133 145 30 529
Cuts at: 1751 1867 2039 2392 2809 2915 3166 3565 3969
Size: 116 172 353 417 106 251 399 404
Cuts at: 3969 4115 4294 4567 4717 4734 4773
Size: 146 179 273 150 17 39
Fragments arranged by size:
529 505 417 404 399 353 273 251
238 179 172 150 146 145 133 116
108 106 63 39 30 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1204 4250 4773
Size: 1204 3046 523
Fragments arranged by size:
3046 1204 523
EcoO109I rG'GnC_Cy
Cuts at: 0 2774 3076 3462 3978 4773
Size: 2774 302 386 516 795
Fragments arranged by size:
2774 795 516 386 302
EcoRI G'AATT_C
Cuts at: 0 544 3289 4773
Size: 544 2745 1484
Fragments arranged by size:
2745 1484 544
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1551 3519 4773
Size: 1551 1968 1254
Fragments arranged by size:
1968 1551 1254
FseI GG_CCGG'CC
Cuts at: 0 4291 4773
Size: 4291 482
FspI TGC'GCA
Cuts at: 0 1303 3124 4773
Size: 1303 1821 1649
Fragments arranged by size:
1821 1649 1303
HaeII r_GCGC'y
Cuts at: 0 1316 4018 4773
Size: 1316 2702 755
Fragments arranged by size:
2702 1316 755
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1021 1053 1429 1461 2089 2121 2682 2714
Size: 1021 32 376 32 628 32 561 32
Cuts at: 2714 2728 2760 2944 2976 3986 4018 4190 4222
Size: 14 32 184 32 1010 32 172 32
Cuts at: 4222 4369 4393 4401 4425 4773
Size: 147 24 8 24 348
Fragments arranged by size:
1021 1010 628 561 376 348 184 172
147 32 32 32 32 32 32 32
32 24 24 14 8
HincII GTy'rAC
Cuts at: 0 19 1933 4773
Size: 19 1914 2840
Fragments arranged by size:
2840 1914 19
HindIII A'AGCT_T
Cuts at: 0 3340 4773
Size: 3340 1433
KasI G'GCGC_C
Cuts at: 0 1312 4773
Size: 1312 3461
KpnI G_GTAC'C
Cuts at: 0 242 3328 4773
Size: 242 3086 1445
Fragments arranged by size:
3086 1445 242
MluI A'CGCG_T
Cuts at: 0 4637 4773
Size: 4637 136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1247 1704 4773
Size: 751 496 457 3069
Fragments arranged by size:
3069 751 496 457
MscI TGG'CCA
Cuts at: 0 1948 3658 4773
Size: 1948 1710 1115
Fragments arranged by size:
1948 1710 1115
MslI CAynn'nnrTG
Cuts at: 0 268 475 1226 3163 3692 3814 4773
Size: 268 207 751 1937 529 122 959
Fragments arranged by size:
1937 959 751 529 268 207 122
MspA1I CmG'CkG
Cuts at: 0 660 823 1109 1241 1761 1865 2560 3084
Size: 660 163 286 132 520 104 695 524
Cuts at: 3084 3741 4605 4773
Size: 657 864 168
Fragments arranged by size:
864 695 660 657 524 520 286 168
163 132 104
NaeI GCC'GGC
Cuts at: 0 1212 1311 3836 4289 4773
Size: 1212 99 2525 453 484
Fragments arranged by size:
2525 1212 484 453 99
NarI GG'CG_CC
Cuts at: 0 1313 4773
Size: 1313 3460
NcoI C'CATG_G
Cuts at: 0 1949 4773
Size: 1949 2824
NgoMIV G'CCGG_C
Cuts at: 0 1210 1309 3834 4287 4773
Size: 1210 99 2525 453 486
Fragments arranged by size:
2525 1210 486 453 99
NruI TCG'CGA
Cuts at: 0 1651 4773
Size: 1651 3122
NspI r_CATG'y
Cuts at: 0 25 55 378 936 4773
Size: 25 30 323 558 3837
Fragments arranged by size:
3837 558 323 30 25
PasI CC'CwG_GG
Cuts at: 0 1560 2884 4773
Size: 1560 1324 1889
Fragments arranged by size:
1889 1560 1324
PciI A'CATG_T
Cuts at: 0 21 4773
Size: 21 4752
PflMI CCAn_nnn'nTGG
Cuts at: 0 201 2829 4773
Size: 201 2628 1944
Fragments arranged by size:
2628 1944 201
PfoI T'CCnGG_A
Cuts at: 0 3143 4750 4773
Size: 3143 1607 23
Fragments arranged by size:
3143 1607 23
PpuMI rG'GwC_Cy
Cuts at: 0 2774 3462 3978 4773
Size: 2774 688 516 795
Fragments arranged by size:
2774 795 688 516
PshAI GACnn'nnGTC
Cuts at: 0 300 2338 4773
Size: 300 2038 2435
Fragments arranged by size:
2435 2038 300
PsiI TTA'TAA
Cuts at: 0 857 4773
Size: 857 3916
PspOMI G'GGCC_C
Cuts at: 0 466 4773
Size: 466 4307
PstI C_TGCA'G
Cuts at: 0 519 4022 4132 4574 4773
Size: 519 3503 110 442 199
Fragments arranged by size:
3503 519 442 199 110
PvuII CAG'CTG
Cuts at: 0 823 1761 2560 3084 3741 4773
Size: 823 938 799 524 657 1032
Fragments arranged by size:
1032 938 823 799 657 524
SacI G_AGCT'C
Cuts at: 0 1255 3143 4773
Size: 1255 1888 1630
Fragments arranged by size:
1888 1630 1255
SacII CC_GC'GG
Cuts at: 0 661 4773
Size: 661 4112
SalI G'TCGA_C
Cuts at: 0 1931 4773
Size: 1931 2842
SanDI GG'GwC_CC
Cuts at: 0 3978 4773
Size: 3978 795
SapI GCTCTTCn'nnn_
Cuts at: 0 939 4773
Size: 939 3834
SbfI CC_TGCA'GG
Cuts at: 0 4574 4773
Size: 4574 199
SfcI C'TryA_G
Cuts at: 0 515 870 3423 4018 4128 4570 4773
Size: 515 355 2553 595 110 442 203
Fragments arranged by size:
2553 595 515 442 355 203 110
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4288 4773
Size: 4288 485
SfoI GGC'GCC
Cuts at: 0 1314 4773
Size: 1314 3459
SmlI C'TyrA_G
Cuts at: 0 291 1831 2686 3343 3580 4657 4773
Size: 291 1540 855 657 237 1077 116
Fragments arranged by size:
1540 1077 855 657 291 237 116
SphI G_CATG'C
Cuts at: 0 55 378 936 4773
Size: 55 323 558 3837
Fragments arranged by size:
3837 558 323 55
SspI AAT'ATT
Cuts at: 0 1799 2189 4773
Size: 1799 390 2584
Fragments arranged by size:
2584 1799 390
StuI AGG'CCT
Cuts at: 0 2663 4694 4773
Size: 2663 2031 79
Fragments arranged by size:
2663 2031 79
StyI C'CwwG_G
Cuts at: 0 692 1949 1959 2847 3458 4773
Size: 692 1257 10 888 611 1315
Fragments arranged by size:
1315 1257 888 692 611 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 258 290 685 1089 1386 1476 1552 1738
Size: 258 32 395 404 297 90 76 186
Cuts at: 1738 2956 3244 3634 4457 4773
Size: 1218 288 390 823 316
Fragments arranged by size:
1218 823 404 395 390 316 297 288
258 186 90 76 32
TatI w'GTAC_w
Cuts at: 0 2495 2992 4773
Size: 2495 497 1781
Fragments arranged by size:
2495 1781 497
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 136 168 3438 3470 3943 3975 4773
Size: 136 32 3270 32 473 32 798
Fragments arranged by size:
3270 798 473 136 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1537 4773
Size: 1537 3236
XbaI T'CTAG_A
Cuts at: 0 3303 4773
Size: 3303 1470
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3718 4773
Size: 3718 1055
XhoI C'TCGA_G
Cuts at: 0 1831 4773
Size: 1831 2942
XmnI GAAnn'nnTTC
Cuts at: 0 1968 3221 4773
Size: 1968 1253 1552
Fragments arranged by size:
1968 1552 1253
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PflMI PfoI PpuMI
PshAI PsiI PspOMI PstI PvuII SacI SacII SalI
SanDI SapI SbfI SfcI SfiI SfoI SmlI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AflII AloI AscI AseI AsiSI AvrII
BaeI BcgI BfrBI BmtI BsaBI BsiWI BspEI BspHI
BsrDI BssHII BstEII BstZ17I ClaI EagI EcoRV FspAI
HpaI MfeI NdeI NheI NotI NsiI PacI PmeI
PmlI PpiI PspXI PsrI PvuI RsrII ScaI SexAI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI XmaI
ZraI