Restriction map for
pF1KE1565
(Linear) (Six-base) MAPSORT of: KIEE1565.seq Check: 1803 from: 1 to: 2196
KIEE1565.orf
With 182 enzymes: SgfI *
January 20, 2017 09:25 ..
AclI AA'CG_TT
Cuts at: 0 1553 2196
Size: 1553 643
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 249 282 2196
Size: 79 170 33 1914
Fragments arranged by size:
1914 170 79 33
AflIII A'CryG_T
Cuts at: 0 539 2148 2196
Size: 539 1609 48
Fragments arranged by size:
1609 539 48
AleI CACnn'nnGTG
Cuts at: 0 1351 2196
Size: 1351 845
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1069 1101 2196
Size: 1069 32 1095
Fragments arranged by size:
1095 1069 32
AlwNI CAG_nnn'CTG
Cuts at: 0 674 1797 2196
Size: 674 1123 399
Fragments arranged by size:
1123 674 399
ApoI r'AATT_y
Cuts at: 0 469 1083 1447 1702 2196
Size: 469 614 364 255 494
Fragments arranged by size:
614 494 469 364 255
AseI AT'TA_AT
Cuts at: 0 1390 2196
Size: 1390 806
AvaI C'yCGr_G
Cuts at: 0 110 2196
Size: 110 2086
BamHI G'GATC_C
Cuts at: 0 832 2196
Size: 832 1364
BanI G'GyrC_C
Cuts at: 0 105 2196
Size: 105 2091
BanII G_rGCy'C
Cuts at: 0 916 1739 2196
Size: 916 823 457
Fragments arranged by size:
916 823 457
BbsI GAAGACnn'nnnn_
Cuts at: 0 437 2196
Size: 437 1759
Bme1580I G_kGCm'C
Cuts at: 0 110 2196
Size: 110 2086
BmgBI CAC'GTC
Cuts at: 0 1725 2169 2196
Size: 1725 444 27
Fragments arranged by size:
1725 444 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 349 2196
Size: 349 1847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 1753 2033 2196
Size: 696 1057 280 163
Fragments arranged by size:
1057 696 280 163
BsaI GGTCTCn'nnnn_
Cuts at: 0 1837 2103 2196
Size: 1837 266 93
Fragments arranged by size:
1837 266 93
BsaAI yAC'GTr
Cuts at: 0 542 2196
Size: 542 1654
BsaBI GATnn'nnATC
Cuts at: 0 1900 2196
Size: 1900 296
BsaHI Gr'CG_yC
Cuts at: 0 116 860 2196
Size: 116 744 1336
Fragments arranged by size:
1336 744 116
BsaWI w'CCGG_w
Cuts at: 0 908 929 1619 2196
Size: 908 21 690 577
Fragments arranged by size:
908 690 577 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 669 699 1726 1756 2196
Size: 669 30 1027 30 440
Fragments arranged by size:
1027 669 440 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1427 2196
Size: 1427 769
BseYI C'CCAG_C
Cuts at: 0 944 1587 2196
Size: 944 643 609
Fragments arranged by size:
944 643 609
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1798 2089 2191 2196
Size: 274 1524 291 102 5
Fragments arranged by size:
1524 291 274 102 5
BsiHKAI G_wGCw'C
Cuts at: 0 738 916 2196
Size: 738 178 1280
Fragments arranged by size:
1280 738 178
BsmI GAATG_Cn'
Cuts at: 0 807 2196
Size: 807 1389
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1708 2196
Size: 1708 488
Bsp1286I G_dGCh'C
Cuts at: 0 110 738 916 1739 2196
Size: 110 628 178 823 457
Fragments arranged by size:
823 628 457 178 110
BspEI T'CCGG_A
Cuts at: 0 1619 2196
Size: 1619 577
BspHI T'CATG_A
Cuts at: 0 407 1535 2196
Size: 407 1128 661
Fragments arranged by size:
1128 661 407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 86 131 2196
Size: 86 45 2065
Fragments arranged by size:
2065 86 45
BsrDI GCAATG_nn'
Cuts at: 0 800 2196
Size: 800 1396
BsrFI r'CCGG_y
Cuts at: 0 1774 2087 2196
Size: 1774 313 109
Fragments arranged by size:
1774 313 109
BssSI C'ACGA_G
Cuts at: 0 144 2196
Size: 144 2052
BstBI TT'CG_AA
Cuts at: 0 513 2196
Size: 513 1683
BstXI CCAn_nnnn'nTGG
Cuts at: 0 875 2196
Size: 875 1321
BstYI r'GATC_y
Cuts at: 0 291 832 2196
Size: 291 541 1364
Fragments arranged by size:
1364 541 291
BtgI C'CryG_G
Cuts at: 0 1911 2196
Size: 1911 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1226 2196
Size: 1226 970
BtsI GCAGTG_nn'
Cuts at: 0 1119 2077 2139 2196
Size: 1119 958 62 57
Fragments arranged by size:
1119 958 62 57
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1362 1397 1893 1928 2196
Size: 1362 35 496 35 268
Fragments arranged by size:
1362 496 268 35 35
DraI TTT'AAA
Cuts at: 0 1970 2196
Size: 1970 226
DrdI GACnn_nn'nnGTC
Cuts at: 0 820 2196
Size: 820 1376
EaeI y'GGCC_r
Cuts at: 0 936 1260 1859 2196
Size: 936 324 599 337
Fragments arranged by size:
936 599 337 324
EarI CTCTTCn'nnn_
Cuts at: 0 30 301 556 602 750 1463 2196
Size: 30 271 255 46 148 713 733
Fragments arranged by size:
733 713 271 255 148 46 30
EcoICRI GAG'CTC
Cuts at: 0 914 2196
Size: 914 1282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 249 282 696 1753 2033 2196
Size: 79 170 33 414 1057 280 163
Fragments arranged by size:
1057 414 280 170 163 79 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 160 2196
Size: 160 2036
EcoO109I rG'GnC_Cy
Cuts at: 0 1689 2196
Size: 1689 507
EcoRI G'AATT_C
Cuts at: 0 1083 1702 2196
Size: 1083 619 494
Fragments arranged by size:
1083 619 494
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 151 2196
Size: 151 2045
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 406 438 669 701 1726 1758 2196
Size: 406 32 231 32 1025 32 438
Fragments arranged by size:
1025 438 406 231 32 32 32
HincII GTy'rAC
Cuts at: 0 120 891 1308 1557 2196
Size: 120 771 417 249 639
Fragments arranged by size:
771 639 417 249 120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 810 1143 1574 2196
Size: 810 333 431 622
Fragments arranged by size:
810 622 431 333
MscI TGG'CCA
Cuts at: 0 938 1861 2196
Size: 938 923 335
Fragments arranged by size:
938 923 335
MslI CAynn'nnrTG
Cuts at: 0 355 1031 1262 1351 1942 2003 2153 2196
Size: 355 676 231 89 591 61 150 43
Fragments arranged by size:
676 591 355 231 150 89 61 43
MspA1I CmG'CkG
Cuts at: 0 67 1794 2196
Size: 67 1727 402
Fragments arranged by size:
1727 402 67
NaeI GCC'GGC
Cuts at: 0 1776 2089 2196
Size: 1776 313 107
Fragments arranged by size:
1776 313 107
NdeI CA'TA_TG
Cuts at: 0 276 2196
Size: 276 1920
NgoMIV G'CCGG_C
Cuts at: 0 1774 2087 2196
Size: 1774 313 109
Fragments arranged by size:
1774 313 109
NspI r_CATG'y
Cuts at: 0 480 1221 2152 2196
Size: 480 741 931 44
Fragments arranged by size:
931 741 480 44
PasI CC'CwG_GG
Cuts at: 0 2093 2196
Size: 2093 103
PciI A'CATG_T
Cuts at: 0 2148 2196
Size: 2148 48
PflMI CCAn_nnn'nTGG
Cuts at: 0 674 2196
Size: 674 1522
PfoI T'CCnGG_A
Cuts at: 0 13 704 1061 2010 2196
Size: 13 691 357 949 186
Fragments arranged by size:
949 691 357 186 13
PpuMI rG'GwC_Cy
Cuts at: 0 1689 2196
Size: 1689 507
PshAI GACnn'nnGTC
Cuts at: 0 266 2196
Size: 266 1930
PvuII CAG'CTG
Cuts at: 0 1794 2196
Size: 1794 402
SacI G_AGCT'C
Cuts at: 0 916 2196
Size: 916 1280
SanDI GG'GwC_CC
Cuts at: 0 1689 2196
Size: 1689 507
SapI GCTCTTCn'nnn_
Cuts at: 0 30 2196
Size: 30 2166
SfcI C'TryA_G
Cuts at: 0 362 2196
Size: 362 1834
SmaI CCC'GGG
Cuts at: 0 112 2196
Size: 112 2084
StuI AGG'CCT
Cuts at: 0 1149 2196
Size: 1149 1047
StyI C'CwwG_G
Cuts at: 0 559 984 1097 2196
Size: 559 425 113 1099
Fragments arranged by size:
1099 559 425 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 63 1390 2196
Size: 63 1327 806
Fragments arranged by size:
1327 806 63
TatI w'GTAC_w
Cuts at: 0 1781 2196
Size: 1781 415
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1093 1125 2068 2100 2196
Size: 1093 32 943 32 96
Fragments arranged by size:
1093 943 96 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 459 1047 1557 1854 2196
Size: 459 588 510 297 342
Fragments arranged by size:
588 510 459 342 297
XmaI C'CCGG_G
Cuts at: 0 110 2196
Size: 110 2086
XmnI GAAnn'nnTTC
Cuts at: 0 2038 2196
Size: 2038 158
Enzymes that do cut:
AclI AcuI AflIII AleI AloI AlwNI ApoI AseI
AvaI BamHI BanI BanII BbsI Bme1580I BmgBI BmrI
BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BssSI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII MmeI
MscI MslI MspA1I NaeI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PpuMI PshAI PvuII SacI SanDI
SapI SfcI SmaI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AlfI ApaI ApaLI AscI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmtI BplI Bpu10I BpuEI BsiEI BsiWI BsrBI BsrGI
BssHII BstAPI BstEII BstZ17I Bsu36I ClaI DraIII EagI
EciI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI NarI NcoI NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
ZraI