Restriction map for
pF1KE1600
(Linear) (Six-base) MAPSORT of: KIEE1600.seq Check: 2853 from: 1 to: 6693
KIEE1600.orf
With 182 enzymes: SgfI *
July 13, 2010 13:08 ..
AatII G_ACGT'C
Cuts at: 0 5585 6693
Size: 5585 1108
AccI GT'mk_AC
Cuts at: 0 3164 6521 6693
Size: 3164 3357 172
Fragments arranged by size:
3357 3164 172
Acc65I G'GTAC_C
Cuts at: 0 692 1697 4446 6693
Size: 692 1005 2749 2247
Fragments arranged by size:
2749 2247 1005 692
AclI AA'CG_TT
Cuts at: 0 3335 6693
Size: 3335 3358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3429 3595 5561 5633 6693
Size: 3429 166 1966 72 1060
Fragments arranged by size:
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AfeI AGC'GCT
Cuts at: 0 534 1036 4627 6693
Size: 534 502 3591 2066
Fragments arranged by size:
3591 2066 534 502
AflII C'TTAA_G
Cuts at: 0 5493 6693
Size: 5493 1200
AflIII A'CryG_T
Cuts at: 0 1715 3869 4115 6014 6693
Size: 1715 2154 246 1899 679
Fragments arranged by size:
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AhdI GACnn_n'nnGTC
Cuts at: 0 2306 3256 5109 6693
Size: 2306 950 1853 1584
Fragments arranged by size:
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AleI CACnn'nnGTG
Cuts at: 0 3121 6693
Size: 3121 3572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2660 3757 4372 6097 6693
Size: 2660 1097 615 1725 596
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 1674 2815 3390 6693
Size: 1674 1141 575 3303
Fragments arranged by size:
3303 1674 1141 575
ApaI G_GGCC'C
Cuts at: 0 352 654 970 1990 2402 4186 4249 4387
Size: 352 302 316 1020 412 1784 63 138
Cuts at: 4387 5633 6693
Size: 1246 1060
Fragments arranged by size:
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138 63
ApaLI G'TGCA_C
Cuts at: 0 3652 6693
Size: 3652 3041
ApoI r'AATT_y
Cuts at: 0 5033 5462 6274 6586 6693
Size: 5033 429 812 312 107
Fragments arranged by size:
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AscI GG'CGCG_CC
Cuts at: 0 263 6693
Size: 263 6430
AseI AT'TA_AT
Cuts at: 0 4919 6693
Size: 4919 1774
AvaI C'yCGr_G
Cuts at: 0 345 432 478 607 970 1138 1525 1980
Size: 345 87 46 129 363 168 387 455
Cuts at: 1980 2092 2905 3501 4002 4179 4248 5995 6693
Size: 112 813 596 501 177 69 1747 698
Fragments arranged by size:
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345 177 168 129 112 87 69 46
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1480 1513 6693
Size: 1480 33 5180
Fragments arranged by size:
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BamHI G'GATC_C
Cuts at: 0 1380 3505 6693
Size: 1380 2125 3188
Fragments arranged by size:
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BanI G'GyrC_C
Cuts at: 0 307 442 692 1264 1336 1697 2370 2766
Size: 307 135 250 572 72 361 673 396
Cuts at: 2766 2784 3340 4025 4404 4446 4602 5477 6693
Size: 18 556 685 379 42 156 875 1216
Fragments arranged by size:
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361 307 250 156 135 72 42 18
BanII G_rGCy'C
Cuts at: 0 352 654 907 934 970 1333 1879 1990
Size: 352 302 253 27 36 363 546 111
Cuts at: 1990 2402 3364 3431 3776 3827 4186 4249 4387
Size: 412 962 67 345 51 359 63 138
Cuts at: 4387 5633 5714 6693
Size: 1246 81 979
Fragments arranged by size:
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345 302 253 138 111 81 67 63
51 36 27
BbeI G_GCGC'C
Cuts at: 0 311 446 1268 2770 2788 4408 4606 5481
Size: 311 135 822 1502 18 1620 198 875
Cuts at: 5481 6693
Size: 1212
Fragments arranged by size:
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18
BbsI GAAGACnn'nnnn_
Cuts at: 0 1787 3435 5180 5201 5212 5621 6693
Size: 1787 1648 1745 21 11 409 1072
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 269 994 1442 1553 4325 4521 6693
Size: 269 725 448 111 2772 196 2172
Fragments arranged by size:
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BciVI GTATCCnnnnn_n'
Cuts at: 0 1508 2071 3992 4229 5674 6226 6693
Size: 1508 563 1921 237 1445 552 467
Fragments arranged by size:
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BclI T'GATC_A
Cuts at: 0 582 5079 6693
Size: 582 4497 1614
Fragments arranged by size:
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BfrBI ATG'CAT
Cuts at: 0 1957 2259 2433 5780 6693
Size: 1957 302 174 3347 913
Fragments arranged by size:
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BglI GCCn_nnn'nGGC
Cuts at: 0 560 649 737 859 974 1015 1288 1297
Size: 560 89 88 122 115 41 273 9
Cuts at: 1297 1484 1610 4109 4391 6693
Size: 187 126 2499 282 2302
Fragments arranged by size:
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115 89 88 41 9
BglII A'GATC_T
Cuts at: 0 1741 1824 6693
Size: 1741 83 4869
Fragments arranged by size:
4869 1741 83
BlpI GC'TnA_GC
Cuts at: 0 361 928 1878 5771 6693
Size: 361 567 950 3893 922
Fragments arranged by size:
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Bme1580I G_kGCm'C
Cuts at: 0 352 454 479 654 970 1990 2375 2402
Size: 352 102 25 175 316 1020 385 27
Cuts at: 2402 2471 3656 4028 4186 4249 4387 5633 6121
Size: 69 1185 372 158 63 138 1246 488
Cuts at: 6121 6693
Size: 572
Fragments arranged by size:
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316 175 158 138 102 69 63 27
25
BmgBI CAC'GTC
Cuts at: 0 4491 6693
Size: 4491 2202
BmrI ACTGGGnnnn_n'
Cuts at: 0 1179 1263 1677 2369 2534 3113 3525 4962
Size: 1179 84 414 692 165 579 412 1437
Cuts at: 4962 6313 6450 6693
Size: 1351 137 243
Fragments arranged by size:
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165 137 84
BmtI G_CTAG'C
Cuts at: 0 3008 6556 6693
Size: 3008 3548 137
Fragments arranged by size:
3548 3008 137
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3213 3215 6693
Size: 3213 2 3478
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1466 1956 2331 3463 4076 4296 4581 4654
Size: 1466 490 375 1132 613 220 285 73
Cuts at: 4654 5722 6214 6693
Size: 1068 492 479
Fragments arranged by size:
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285 220 73
Bpu10I CC'TnA_GC
Cuts at: 0 269 994 1442 1553 4325 4521 6693
Size: 269 725 448 111 2772 196 2172
Fragments arranged by size:
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BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2148 2277 3192 3330 6024 6693
Size: 2148 129 915 138 2694 669
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 1392 5051 6190 6693
Size: 1392 3659 1139 503
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 4901 6693
Size: 4901 1792
BsaHI Gr'CG_yC
Cuts at: 0 308 443 1265 2767 2785 4405 4570 4603
Size: 308 135 822 1502 18 1620 165 33
Cuts at: 4603 5252 5478 5582 6693
Size: 649 226 104 1111
Fragments arranged by size:
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135 104 33 18
BsaWI w'CCGG_w
Cuts at: 0 3889 5684 6693
Size: 3889 1795 1009
Fragments arranged by size:
3889 1795 1009
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 616 646 4755 4785 6693
Size: 616 30 4109 30 1908
Fragments arranged by size:
4109 1908 616 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 785 788 791 803 806 809 812 815
Size: 785 3 3 12 3 3 3 3
Cuts at: 815 818 821 836 1742 3003 3081 3439 5152
Size: 3 3 15 906 1261 78 358 1713
Cuts at: 5152 5194 5203 5459 5723 5953 6242 6693
Size: 42 9 256 264 230 289 451
Fragments arranged by size:
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256 230 78 42 15 12 9 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 568 1102 1153 1688 2355 2491 2995 3132
Size: 568 534 51 535 667 136 504 137
Cuts at: 3132 3588 4606 4611 5062 6693
Size: 456 1018 5 451 1631
Fragments arranged by size:
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451 137 136 51 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3374 5380 5392 6088 6418 6552 6693
Size: 3374 2006 12 696 330 134 141
Fragments arranged by size:
3374 2006 696 330 141 134 12
BsiEI CG_ry'CG
Cuts at: 0 420 470 503 564 854 1007 1235 1301
Size: 420 50 33 61 290 153 228 66
Cuts at: 1301 3168 3954 6693
Size: 1867 786 2739
Fragments arranged by size:
2739 1867 786 420 290 228 153 66
61 50 33
BsiHKAI G_wGCw'C
Cuts at: 0 431 934 1879 3364 3656 5432 5919 6693
Size: 431 503 945 1485 292 1776 487 774
Fragments arranged by size:
1776 1485 945 774 503 487 431 292
BsmI GAATG_Cn'
Cuts at: 0 1547 2486 6693
Size: 1547 939 4207
Fragments arranged by size:
4207 1547 939
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5717 6693
Size: 5717 976
Bsp1286I G_dGCh'C
Cuts at: 0 352 431 454 479 654 907 934 970
Size: 352 79 23 25 175 253 27 36
Cuts at: 970 1333 1879 1990 2375 2402 2471 3364 3431
Size: 363 546 111 385 27 69 893 67
Cuts at: 3431 3656 3776 3827 4028 4186 4249 4387 5432
Size: 225 120 51 201 158 63 138 1045
Cuts at: 5432 5633 5714 5919 6121 6693
Size: 201 81 205 202 572
Fragments arranged by size:
1045 893 572 546 385 363 352 253
225 205 202 201 201 175 158 138
120 111 81 79 69 67 63 51
36 27 27 25 23
BspEI T'CCGG_A
Cuts at: 0 3889 6693
Size: 3889 2804
BspHI T'CATG_A
Cuts at: 0 4467 6693
Size: 4467 2226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2978 5494 6693
Size: 2978 2516 1199
Fragments arranged by size:
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BsrBI CCG'CTC
Cuts at: 0 548 832 6581 6693
Size: 548 284 5749 112
Fragments arranged by size:
5749 548 284 112
BsrDI GCAATG_nn'
Cuts at: 0 3902 4356 5605 5832 6693
Size: 3902 454 1249 227 861
Fragments arranged by size:
3902 1249 861 454 227
BsrFI r'CCGG_y
Cuts at: 0 408 465 731 1009 1042 6605 6693
Size: 408 57 266 278 33 5563 88
Fragments arranged by size:
5563 408 278 266 88 57 33
BsrGI T'GTAC_A
Cuts at: 0 3872 4118 4154 6693
Size: 3872 246 36 2539
Fragments arranged by size:
3872 2539 246 36
BssHII G'CGCG_C
Cuts at: 0 263 1157 6693
Size: 263 894 5536
Fragments arranged by size:
5536 894 263
BssSI C'ACGA_G
Cuts at: 0 1902 4105 5893 6598 6693
Size: 1902 2203 1788 705 95
Fragments arranged by size:
2203 1902 1788 705 95
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1563 1674 1888 6693
Size: 1563 111 214 4805
Fragments arranged by size:
4805 1563 214 111
BstEII G'GTnAC_C
Cuts at: 0 3568 4719 6693
Size: 3568 1151 1974
Fragments arranged by size:
3568 1974 1151
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2434 2458 3538 4975 6693
Size: 2434 24 1080 1437 1718
Fragments arranged by size:
2434 1718 1437 1080 24
BstYI r'GATC_y
Cuts at: 0 1360 1380 1741 1824 3505 4256 6138 6693
Size: 1360 20 361 83 1681 751 1882 555
Fragments arranged by size:
1882 1681 1360 751 555 361 83 20
Bsu36I CC'TnA_GG
Cuts at: 0 4847 6693
Size: 4847 1846
BtgI C'CryG_G
Cuts at: 0 854 911 1235 1352 1532 1853 2792 3221
Size: 854 57 324 117 180 321 939 429
Cuts at: 3221 3551 4700 6338 6557 6693
Size: 330 1149 1638 219 136
Fragments arranged by size:
1638 1149 939 854 429 330 324 321
219 180 136 117 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1339 5217 5231 5730 5790 6693
Size: 1339 3878 14 499 60 903
Fragments arranged by size:
3878 1339 903 499 60 14
BtsI GCAGTG_nn'
Cuts at: 0 2636 3357 4579 6693
Size: 2636 721 1222 2114
Fragments arranged by size:
2636 2114 1222 721
ClaI AT'CG_AT
Cuts at: 0 5576 6693
Size: 5576 1117
DrdI GACnn_nn'nnGTC
Cuts at: 0 3531 4575 6693
Size: 3531 1044 2118
Fragments arranged by size:
3531 2118 1044
EaeI y'GGCC_r
Cuts at: 0 330 417 500 561 851 1004 1044 1221
Size: 330 87 83 61 290 153 40 177
Cuts at: 1221 1232 1298 1535 3058 3606 5596 6029 6245
Size: 11 66 237 1523 548 1990 433 216
Cuts at: 6245 6539 6560 6693
Size: 294 21 133
Fragments arranged by size:
1990 1523 548 433 330 294 290 237
216 177 153 133 87 83 66 61
40 21 11
EagI C'GGCC_G
Cuts at: 0 417 500 561 851 1004 1232 1298 6693
Size: 417 83 61 290 153 228 66 5395
Fragments arranged by size:
5395 417 290 228 153 83 66 61
EarI CTCTTCn'nnn_
Cuts at: 0 1390 3182 5537 6693
Size: 1390 1792 2355 1156
Fragments arranged by size:
2355 1792 1390 1156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 608 618 624 782 6693
Size: 608 10 6 158 5911
Fragments arranged by size:
5911 608 158 10 6
EcoICRI GAG'CTC
Cuts at: 0 932 1877 3362 6693
Size: 932 945 1485 3331
Fragments arranged by size:
3331 1485 945 932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1466 1956 2331 3429 3463 3595 4076 4296
Size: 1466 490 375 1098 34 132 481 220
Cuts at: 4296 4581 4654 5561 5633 5722 6214 6693
Size: 285 73 907 72 89 492 479
Fragments arranged by size:
1466 1098 907 492 490 481 479 375
285 220 132 89 73 72 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 997 4227 4415 6693
Size: 997 3230 188 2278
Fragments arranged by size:
3230 2278 997 188
EcoO109I rG'GnC_Cy
Cuts at: 0 650 651 967 1986 2398 3783 3994 4245
Size: 650 1 316 1019 412 1385 211 251
Cuts at: 4245 4321 4383 4384 5630 6157 6693
Size: 76 62 1 1246 527 536
Fragments arranged by size:
1385 1246 1019 650 536 527 412 316
251 211 76 62 1 1
EcoRI G'AATT_C
Cuts at: 0 5033 6586 6693
Size: 5033 1553 107
Fragments arranged by size:
5033 1553 107
EcoRV GAT'ATC
Cuts at: 0 4836 6618 6626 6693
Size: 4836 1782 8 67
Fragments arranged by size:
4836 1782 67 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2318 3804 5673 6693
Size: 2318 1486 1869 1020
Fragments arranged by size:
2318 1869 1486 1020
HaeII r_GCGC'y
Cuts at: 0 311 446 536 1038 1268 2770 2788 3924
Size: 311 135 90 502 230 1502 18 1136
Cuts at: 3924 4104 4408 4606 4629 5481 6346 6693
Size: 180 304 198 23 852 865 347
Fragments arranged by size:
1502 1136 865 852 502 347 311 304
230 198 180 135 90 23 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1068 1100 1554 1586 2292 2324 2821 2853
Size: 1068 32 454 32 706 32 497 32
Cuts at: 2853 2899 2931 3242 3274 3649 3681 4449 4481
Size: 46 32 311 32 375 32 768 32
Cuts at: 4481 5095 5127 5572 5604 6693
Size: 614 32 445 32 1089
Fragments arranged by size:
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375 311 46 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3165 3536 5346 6693
Size: 3165 371 1810 1347
Fragments arranged by size:
3165 1810 1347 371
HindIII A'AGCT_T
Cuts at: 0 6426 6693
Size: 6426 267
KasI G'GCGC_C
Cuts at: 0 307 442 1264 2766 2784 4404 4602 5477
Size: 307 135 822 1502 18 1620 198 875
Cuts at: 5477 6693
Size: 1216
Fragments arranged by size:
1620 1502 1216 875 822 307 198 135
18
KpnI G_GTAC'C
Cuts at: 0 696 1701 4450 6693
Size: 696 1005 2749 2243
Fragments arranged by size:
2749 2243 1005 696
MfeI C'AATT_G
Cuts at: 0 1502 6693
Size: 1502 5191
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1498 3273 6172 6693
Size: 254 1244 1775 2899 521
Fragments arranged by size:
2899 1775 1244 521 254
MscI TGG'CCA
Cuts at: 0 3060 6031 6247 6562 6693
Size: 3060 2971 216 315 131
Fragments arranged by size:
3060 2971 315 216 131
MslI CAynn'nnrTG
Cuts at: 0 89 110 961 3026 3121 3181 3739 5785
Size: 89 21 851 2065 95 60 558 2046
Cuts at: 5785 6394 6487 6693
Size: 609 93 206
Fragments arranged by size:
2065 2046 851 609 558 206 95 93
89 60 21
MspA1I CmG'CkG
Cuts at: 0 357 785 856 913 1089 1237 1279 1494
Size: 357 428 71 57 176 148 42 215
Cuts at: 1494 2390 2491 4299 5257 6631 6693
Size: 896 101 1808 958 1374 62
Fragments arranged by size:
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148 101 71 62 57 42
NaeI GCC'GGC
Cuts at: 0 410 733 1011 1044 6693
Size: 410 323 278 33 5649
Fragments arranged by size:
5649 410 323 278 33
NarI GG'CG_CC
Cuts at: 0 308 443 1265 2767 2785 4405 4603 5478
Size: 308 135 822 1502 18 1620 198 875
Cuts at: 5478 6693
Size: 1215
Fragments arranged by size:
1620 1502 1215 875 822 308 198 135
18
NcoI C'CATG_G
Cuts at: 0 1352 1532 2792 3551 6338 6557 6693
Size: 1352 180 1260 759 2787 219 136
Fragments arranged by size:
2787 1352 1260 759 219 180 136
NdeI CA'TA_TG
Cuts at: 0 4070 6693
Size: 4070 2623
NgoMIV G'CCGG_C
Cuts at: 0 408 731 1009 1042 6693
Size: 408 323 278 33 5651
Fragments arranged by size:
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NheI G'CTAG_C
Cuts at: 0 3004 6552 6693
Size: 3004 3548 141
Fragments arranged by size:
3548 3004 141
NotI GC'GGCC_GC
Cuts at: 0 417 561 851 1004 1232 1298 6693
Size: 417 144 290 153 228 66 5395
Fragments arranged by size:
5395 417 290 228 153 144 66
NsiI A_TGCA'T
Cuts at: 0 1959 2261 2435 5782 6693
Size: 1959 302 174 3347 911
Fragments arranged by size:
3347 1959 911 302 174
NspI r_CATG'y
Cuts at: 0 1719 1957 2263 2556 3744 3864 3873 4083
Size: 1719 238 306 293 1188 120 9 210
Cuts at: 4083 4119 4128 4647 6492 6693
Size: 36 9 519 1845 201
Fragments arranged by size:
1845 1719 1188 519 306 293 238 210
201 120 36 9 9
PasI CC'CwG_GG
Cuts at: 0 447 1365 2395 2472 5626 6693
Size: 447 918 1030 77 3154 1067
Fragments arranged by size:
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PciI A'CATG_T
Cuts at: 0 1715 3869 4115 6693
Size: 1715 2154 246 2578
Fragments arranged by size:
2578 2154 1715 246
PflMI CCAn_nnn'nTGG
Cuts at: 0 2491 2515 5115 6693
Size: 2491 24 2600 1578
Fragments arranged by size:
2600 2491 1578 24
PfoI T'CCnGG_A
Cuts at: 0 506 2514 6218 6693
Size: 506 2008 3704 475
Fragments arranged by size:
3704 2008 506 475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1728 1760 2728 2760 6693
Size: 1728 32 968 32 3933
Fragments arranged by size:
3933 1728 968 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 3783 6157 6693
Size: 3783 2374 536
Fragments arranged by size:
3783 2374 536
PspOMI G'GGCC_C
Cuts at: 0 348 650 966 1986 2398 4182 4245 4383
Size: 348 302 316 1020 412 1784 63 138
Cuts at: 4383 5629 6693
Size: 1246 1064
Fragments arranged by size:
1784 1246 1064 1020 412 348 316 302
138 63
PspXI vC'TCGA_Gb
Cuts at: 0 5995 6693
Size: 5995 698
PstI C_TGCA'G
Cuts at: 0 677 4304 5262 5968 6129 6693
Size: 677 3627 958 706 161 564
Fragments arranged by size:
3627 958 706 677 564 161
PvuII CAG'CTG
Cuts at: 0 1089 2491 6631 6693
Size: 1089 1402 4140 62
Fragments arranged by size:
4140 1402 1089 62
RsrII CG'GwC_CG
Cuts at: 0 1345 4188 6693
Size: 1345 2843 2505
Fragments arranged by size:
2843 2505 1345
SacI G_AGCT'C
Cuts at: 0 934 1879 3364 6693
Size: 934 945 1485 3329
Fragments arranged by size:
3329 1485 945 934
SacII CC_GC'GG
Cuts at: 0 857 914 1238 6693
Size: 857 57 324 5455
Fragments arranged by size:
5455 857 324 57
SalI G'TCGA_C
Cuts at: 0 3163 6693
Size: 3163 3530
SanDI GG'GwC_CC
Cuts at: 0 6157 6693
Size: 6157 536
SapI GCTCTTCn'nnn_
Cuts at: 0 5537 6693
Size: 5537 1156
ScaI AGT'ACT
Cuts at: 0 5002 6693
Size: 5002 1691
SfcI C'TryA_G
Cuts at: 0 142 673 726 2015 2385 2721 2775 2928
Size: 142 531 53 1289 370 336 54 153
Cuts at: 2928 4224 4300 5258 5964 6125 6693
Size: 1296 76 958 706 161 568
Fragments arranged by size:
1296 1289 958 706 568 531 370 336
161 153 142 76 54 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 560 1297 1484 4391 6693
Size: 560 737 187 2907 2302
Fragments arranged by size:
2907 2302 737 560 187
SfoI GGC'GCC
Cuts at: 0 309 444 1266 2768 2786 4406 4604 5479
Size: 309 135 822 1502 18 1620 198 875
Cuts at: 5479 6693
Size: 1214
Fragments arranged by size:
1620 1502 1214 875 822 309 198 135
18
SmaI CCC'GGG
Cuts at: 0 347 434 480 609 972 1527 1982 2094
Size: 347 87 46 129 363 555 455 112
Cuts at: 2094 4250 6693
Size: 2156 2443
Fragments arranged by size:
2443 2156 555 455 363 347 129 112
87 46
SmlI C'TyrA_G
Cuts at: 0 2127 2292 3207 3345 5493 5995 6003 6693
Size: 2127 165 915 138 2148 502 8 690
Fragments arranged by size:
2148 2127 915 690 502 165 138 8
SphI G_CATG'C
Cuts at: 0 2263 3744 3864 6693
Size: 2263 1481 120 2829
Fragments arranged by size:
2829 2263 1481 120
SspI AAT'ATT
Cuts at: 0 4923 5796 6693
Size: 4923 873 897
Fragments arranged by size:
4923 897 873
StuI AGG'CCT
Cuts at: 0 5855 6693
Size: 5855 838
StyI C'CwwG_G
Cuts at: 0 311 1352 1532 1911 2792 3551 3990 5114
Size: 311 1041 180 379 881 759 439 1124
Cuts at: 5114 6338 6363 6542 6557 6693
Size: 1224 25 179 15 136
Fragments arranged by size:
1224 1124 1041 881 759 439 379 311
180 179 136 25 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1362 3182 3968 5363 5653 6156 6693
Size: 1362 1820 786 1395 290 503 537
Fragments arranged by size:
1820 1395 1362 786 537 503 290
TatI w'GTAC_w
Cuts at: 0 1142 3640 3872 4118 4130 4154 5000 6669
Size: 1142 2498 232 246 12 24 846 1669
Cuts at: 6669 6693
Size: 24
Fragments arranged by size:
2498 1669 1142 846 246 232 24 24
12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 616 648 4256 4288 4755 4787 6693
Size: 616 32 3608 32 467 32 1906
Fragments arranged by size:
3608 1906 616 467 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2035 2260 6564 6693
Size: 2035 225 4304 129
Fragments arranged by size:
4304 2035 225 129
XhoI C'TCGA_G
Cuts at: 0 5995 6693
Size: 5995 698
XmaI C'CCGG_G
Cuts at: 0 345 432 478 607 970 1525 1980 2092
Size: 345 87 46 129 363 555 455 112
Cuts at: 2092 4248 6693
Size: 2156 2445
Fragments arranged by size:
2445 2156 555 455 363 345 129 112
87 46
ZraI GAC'GTC
Cuts at: 0 5583 6693
Size: 5583 1110
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PpiI PpuMI PspOMI PspXI PstI PvuII
RsrII SacI SacII SalI SanDI SapI ScaI SfcI
SfiI SfoI SmaI SmlI SphI SspI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AgeI AloI AsiSI AvrII BcgI BsaBI BsiWI
BstBI BstZ17I CspCI DraI DraIII FseI FspI FspAI
HpaI MluI NruI PacI PmeI PmlI PshAI PsiI
PsrI PvuI SbfI SexAI SgfI SgrAI SnaBI SpeI
SrfI SwaI Tth111I XbaI XmnI