(Linear) (Six-base) MAPSORT of: KIEE1617.seq  Check: 2781  from: 1  to: 381



KIEE1617.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AfeI AGC'GCT

Cuts at:      0     262     381 
   Size:        262     119
 
ApoI r'AATT_y

Cuts at:      0     192     381 
   Size:        192     189
 
BanI G'GyrC_C

Cuts at:      0     346     381 
   Size:        346      35
 
Bme1580I G_kGCm'C

Cuts at:      0     349     381 
   Size:        349      32
 
BsaWI w'CCGG_w

Cuts at:      0     153     381 
   Size:        153     228
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     358     381 
   Size:        358      23
 
Bsp1286I G_dGCh'C

Cuts at:      0     349     381 
   Size:        349      32
 
BspEI T'CCGG_A

Cuts at:      0     153     381 
   Size:        153     228
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     135     381 
   Size:        135     246
 
BstBI TT'CG_AA

Cuts at:      0     172     381 
   Size:        172     209
 
BtsI GCAGTG_nn'

Cuts at:      0     302     381 
   Size:        302      79
 
EarI CTCTTCn'nnn_

Cuts at:      0     174     381 
   Size:        174     207
 
EcoO109I rG'GnC_Cy

Cuts at:      0      98     381 
   Size:         98     283
 
EcoRI G'AATT_C

Cuts at:      0     192     381 
   Size:        192     189
 
EcoRV GAT'ATC

Cuts at:      0     210     381 
   Size:        210     171
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      60     381 
   Size:         60     321
 
HaeII r_GCGC'y

Cuts at:      0     264     381 
   Size:        264     117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30     381 
   Size:         30     351
 
NspI r_CATG'y

Cuts at:      0     334     381 
   Size:        334      47
 
PpuMI rG'GwC_Cy

Cuts at:      0      98     381 
   Size:         98     283
 
SfcI C'TryA_G

Cuts at:      0     252     381 
   Size:        252     129
 
StyI C'CwwG_G

Cuts at:      0     350     381 
   Size:        350      31
 
TatI w'GTAC_w

Cuts at:      0     365     381 
   Size:        365      16
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     157     381 
   Size:        157     224
 
XmnI GAAnn'nnTTC

Cuts at:      0     196     381 
   Size:        196     185
 

 Enzymes that do cut:

     AfeI     ApoI     BanI Bme1580I    BsaWI    BsaXI Bsp1286I    BspEI
    BspMI    BstBI     BtsI     EarI EcoO109I    EcoRI    EcoRV     FalI
    HaeII    Hin4I     NspI    PpuMI     SfcI     StyI     TatI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     ZraI