(Linear) (Six-base) MAPSORT of: KIEE1686.seq  Check: 7198  from: 1  to: 594



KIEE1686.orf

 With 182 enzymes: SgfI * 

                        June 16, 2011 10:51  ..

AvrII C'CTAG_G

Cuts at:      0       6     594 
   Size:          6     588
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     567     594 
   Size:        567      27
 
BanII G_rGCy'C

Cuts at:      0      45     594 
   Size:         45     549
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     197     245     552     594 
   Size:        197      48     307      42

  Fragments arranged by size: 

                 307     197      48      42
 
BglI GCCn_nnn'nGGC

Cuts at:      0     326     594 
   Size:        326     268
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      16     594 
   Size:         16     578
 
BseYI C'CCAG_C

Cuts at:      0      21      84     339     594 
   Size:         21      63     255     255

  Fragments arranged by size: 

                 255     255      63      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     397     594 
   Size:        397     197
 
BsmI GAATG_Cn'

Cuts at:      0     180     594 
   Size:        180     414
 
Bsp1286I G_dGCh'C

Cuts at:      0      45     397     594 
   Size:         45     352     197

  Fragments arranged by size: 

                 352     197      45
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     175     594 
   Size:        175     419
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     534     594 
   Size:        534      60
 
EcoO109I rG'GnC_Cy

Cuts at:      0     105     594 
   Size:        105     489
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     528     594 
   Size:        528      66
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     594 
   Size:         55      32     507

  Fragments arranged by size: 

                 507      55      32
 
NspI r_CATG'y

Cuts at:      0      52     415     594 
   Size:         52     363     179

  Fragments arranged by size: 

                 363     179      52
 
PfoI T'CCnGG_A

Cuts at:      0     125     594 
   Size:        125     469
 
PpuMI rG'GwC_Cy

Cuts at:      0     105     594 
   Size:        105     489
 
PstI C_TGCA'G

Cuts at:      0     116     594 
   Size:        116     478
 
ScaI AGT'ACT

Cuts at:      0     454     594 
   Size:        454     140
 
SfcI C'TryA_G

Cuts at:      0     112     594 
   Size:        112     482
 
SphI G_CATG'C

Cuts at:      0      52     415     594 
   Size:         52     363     179

  Fragments arranged by size: 

                 363     179      52
 
StuI AGG'CCT

Cuts at:      0     164     594 
   Size:        164     430
 
StyI C'CwwG_G

Cuts at:      0       6     482     594 
   Size:          6     476     112

  Fragments arranged by size: 

                 476     112       6
 
TatI w'GTAC_w

Cuts at:      0     452     594 
   Size:        452     142
 

 Enzymes that do cut:

    AvrII     BaeI    BanII     BbsI     BglI     BsaI    BseYI  BsiHKAI
     BsmI Bsp1286I   BstAPI    BstXI EcoO109I     FalI    Hin4I     NspI
     PfoI    PpuMI     PstI     ScaI     SfcI     SphI     StuI     StyI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI