Restriction map for
pF1KE1722
(Linear) (Six-base) MAPSORT of: KIEE1722.seq Check: 2031 from: 1 to: 699
KIEE1722.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 682 699
Size: 682 17
AhdI GACnn_n'nnGTC
Cuts at: 0 375 699
Size: 375 324
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 130 699
Size: 130 569
AlwNI CAG_nnn'CTG
Cuts at: 0 327 699
Size: 327 372
ApaI G_GGCC'C
Cuts at: 0 312 418 525 699
Size: 312 106 107 174
Fragments arranged by size:
312 174 107 106
BanI G'GyrC_C
Cuts at: 0 22 142 659 699
Size: 22 120 517 40
Fragments arranged by size:
517 120 40 22
BanII G_rGCy'C
Cuts at: 0 55 84 312 418 525 629 699
Size: 55 29 228 106 107 104 70
Fragments arranged by size:
228 107 106 104 70 55 29
BbeI G_GCGC'C
Cuts at: 0 146 699
Size: 146 553
BbsI GAAGACnn'nnnn_
Cuts at: 0 348 658 699
Size: 348 310 41
Fragments arranged by size:
348 310 41
BglI GCCn_nnn'nGGC
Cuts at: 0 683 699
Size: 683 16
Bme1580I G_kGCm'C
Cuts at: 0 312 418 525 699
Size: 312 106 107 174
Fragments arranged by size:
312 174 107 106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 699
Size: 497 202
Bpu10I CC'TnA_GC
Cuts at: 0 83 699
Size: 83 616
BsaI GGTCTCn'nnnn_
Cuts at: 0 529 699
Size: 529 170
BsaHI Gr'CG_yC
Cuts at: 0 143 353 699
Size: 143 210 346
Fragments arranged by size:
346 210 143
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 580 699
Size: 580 119
BseYI C'CCAG_C
Cuts at: 0 348 428 515 699
Size: 348 80 87 184
Fragments arranged by size:
348 184 87 80
BsmI GAATG_Cn'
Cuts at: 0 266 699
Size: 266 433
BsmBI CGTCTCn'nnnn_
Cuts at: 0 383 699
Size: 383 316
Bsp1286I G_dGCh'C
Cuts at: 0 55 84 312 418 525 629 699
Size: 55 29 228 106 107 104 70
Fragments arranged by size:
228 107 106 104 70 55 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 682 699
Size: 682 17
BssSI C'ACGA_G
Cuts at: 0 442 699
Size: 442 257
BstXI CCAn_nnnn'nTGG
Cuts at: 0 153 699
Size: 153 546
BstYI r'GATC_y
Cuts at: 0 593 699
Size: 593 106
BtgI C'CryG_G
Cuts at: 0 614 629 699
Size: 614 15 70
Fragments arranged by size:
614 70 15
BtsI GCAGTG_nn'
Cuts at: 0 470 699
Size: 470 229
DraIII CAC_nnn'GTG
Cuts at: 0 531 699
Size: 531 168
EaeI y'GGCC_r
Cuts at: 0 41 542 699
Size: 41 501 157
Fragments arranged by size:
501 157 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 699
Size: 497 202
EcoO109I rG'GnC_Cy
Cuts at: 0 308 309 414 415 521 638 699
Size: 308 1 105 1 106 117 61
Fragments arranged by size:
308 117 106 105 61 1 1
HaeII r_GCGC'y
Cuts at: 0 146 699
Size: 146 553
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 358 361 390 393 699
Size: 358 3 29 3 306
Fragments arranged by size:
358 306 29 3 3
HincII GTy'rAC
Cuts at: 0 245 699
Size: 245 454
KasI G'GCGC_C
Cuts at: 0 142 699
Size: 142 557
MscI TGG'CCA
Cuts at: 0 43 699
Size: 43 656
MspA1I CmG'CkG
Cuts at: 0 114 180 390 493 515 699
Size: 114 66 210 103 22 184
Fragments arranged by size:
210 184 114 103 66 22
NarI GG'CG_CC
Cuts at: 0 143 699
Size: 143 556
NcoI C'CATG_G
Cuts at: 0 614 629 699
Size: 614 15 70
Fragments arranged by size:
614 70 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 327 531 699
Size: 327 204 168
Fragments arranged by size:
327 204 168
PfoI T'CCnGG_A
Cuts at: 0 221 605 699
Size: 221 384 94
Fragments arranged by size:
384 221 94
PpuMI rG'GwC_Cy
Cuts at: 0 638 699
Size: 638 61
PspOMI G'GGCC_C
Cuts at: 0 308 414 521 699
Size: 308 106 107 178
Fragments arranged by size:
308 178 107 106
PstI C_TGCA'G
Cuts at: 0 395 501 699
Size: 395 106 198
Fragments arranged by size:
395 198 106
PvuII CAG'CTG
Cuts at: 0 114 180 390 515 699
Size: 114 66 210 125 184
Fragments arranged by size:
210 184 125 114 66
SfcI C'TryA_G
Cuts at: 0 391 497 642 699
Size: 391 106 145 57
Fragments arranged by size:
391 145 106 57
SfoI GGC'GCC
Cuts at: 0 144 699
Size: 144 555
StuI AGG'CCT
Cuts at: 0 571 699
Size: 571 128
StyI C'CwwG_G
Cuts at: 0 614 629 699
Size: 614 15 70
Fragments arranged by size:
614 70 15
TatI w'GTAC_w
Cuts at: 0 122 699
Size: 122 577
Enzymes that do cut:
AarI AhdI AlfI AlwNI ApaI BanI BanII BbeI
BbsI BglI Bme1580I BpmI Bpu10I BsaI BsaHI BseRI
BseYI BsmI BsmBI Bsp1286I BspMI BssSI BstXI BstYI
BtgI BtsI DraIII EaeI Eco57MI EcoO109I HaeII Hin4I
HincII KasI MscI MspA1I NarI NcoI PflMI PfoI
PpuMI PspOMI PstI PvuII SfcI SfoI StuI StyI
TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MmeI MslI NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI