Restriction map for
pF1KE1731
(Linear) (Six-base) MAPSORT of: KIEE1731.seq Check: 9373 from: 1 to: 717
KIEE1731.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 0 104 717
Size: 104 613
Acc65I G'GTAC_C
Cuts at: 0 284 717
Size: 284 433
AflIII A'CryG_T
Cuts at: 0 461 717
Size: 461 256
ApaI G_GGCC'C
Cuts at: 0 61 429 717
Size: 61 368 288
Fragments arranged by size:
368 288 61
AvaI C'yCGr_G
Cuts at: 0 177 717
Size: 177 540
BamHI G'GATC_C
Cuts at: 0 359 717
Size: 359 358
BanI G'GyrC_C
Cuts at: 0 123 131 284 717
Size: 123 8 153 433
Fragments arranged by size:
433 153 123 8
BanII G_rGCy'C
Cuts at: 0 41 61 69 429 649 665 694 717
Size: 41 20 8 360 220 16 29 23
Fragments arranged by size:
360 220 41 29 23 20 16 8
BbeI G_GCGC'C
Cuts at: 0 127 135 717
Size: 127 8 582
Fragments arranged by size:
582 127 8
BglI GCCn_nnn'nGGC
Cuts at: 0 45 98 627 717
Size: 45 53 529 90
Fragments arranged by size:
529 90 53 45
BlpI GC'TnA_GC
Cuts at: 0 25 717
Size: 25 692
Bme1580I G_kGCm'C
Cuts at: 0 61 429 717
Size: 61 368 288
Fragments arranged by size:
368 288 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 697 717
Size: 697 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 151 223 679 717
Size: 55 96 72 456 38
Fragments arranged by size:
456 96 72 55 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 274 717
Size: 274 443
BsaAI yAC'GTr
Cuts at: 0 383 462 717
Size: 383 79 255
Fragments arranged by size:
383 255 79
BsaHI Gr'CG_yC
Cuts at: 0 101 124 132 717
Size: 101 23 8 585
Fragments arranged by size:
585 101 23 8
BsaWI w'CCGG_w
Cuts at: 0 401 717
Size: 401 316
BseYI C'CCAG_C
Cuts at: 0 369 439 695 717
Size: 369 70 256 22
Fragments arranged by size:
369 256 70 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 717
Size: 157 560
BsiEI CG_ry'CG
Cuts at: 0 93 186 717
Size: 93 93 531
Fragments arranged by size:
531 93 93
BsiHKAI G_wGCw'C
Cuts at: 0 113 329 717
Size: 113 216 388
Fragments arranged by size:
388 216 113
Bsp1286I G_dGCh'C
Cuts at: 0 41 61 69 113 329 429 649 665
Size: 41 20 8 44 216 100 220 16
Cuts at: 665 694 717
Size: 29 23
Fragments arranged by size:
220 216 100 44 41 29 23 20
16 8
BsrBI CCG'CTC
Cuts at: 0 182 357 717
Size: 182 175 360
Fragments arranged by size:
360 182 175
BsrFI r'CCGG_y
Cuts at: 0 431 482 717
Size: 431 51 235
Fragments arranged by size:
431 235 51
BssSI C'ACGA_G
Cuts at: 0 364 562 717
Size: 364 198 155
Fragments arranged by size:
364 198 155
BstYI r'GATC_y
Cuts at: 0 359 717
Size: 359 358
BtgI C'CryG_G
Cuts at: 0 93 717
Size: 93 624
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 95 717
Size: 95 622
BtsI GCAGTG_nn'
Cuts at: 0 245 717
Size: 245 472
EaeI y'GGCC_r
Cuts at: 0 90 183 257 290 717
Size: 90 93 74 33 427
Fragments arranged by size:
427 93 90 74 33
EagI C'GGCC_G
Cuts at: 0 90 183 717
Size: 90 93 534
Fragments arranged by size:
534 93 90
EarI CTCTTCn'nnn_
Cuts at: 0 277 717
Size: 277 440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 151 223 679 717
Size: 55 96 72 456 38
Fragments arranged by size:
456 96 72 55 38
EcoO109I rG'GnC_Cy
Cuts at: 0 58 425 717
Size: 58 367 292
Fragments arranged by size:
367 292 58
FseI GG_CCGG'CC
Cuts at: 0 486 717
Size: 486 231
FspI TGC'GCA
Cuts at: 0 713 717
Size: 713 4
FspAI rTGC'GCAy
Cuts at: 0 713 717
Size: 713 4
HaeII r_GCGC'y
Cuts at: 0 127 135 215 338 446 717
Size: 127 8 80 123 108 271
Fragments arranged by size:
271 127 123 108 80 8
HincII GTy'rAC
Cuts at: 0 192 717
Size: 192 525
KasI G'GCGC_C
Cuts at: 0 123 131 717
Size: 123 8 586
Fragments arranged by size:
586 123 8
KpnI G_GTAC'C
Cuts at: 0 288 717
Size: 288 429
MscI TGG'CCA
Cuts at: 0 259 292 717
Size: 259 33 425
Fragments arranged by size:
425 259 33
MspA1I CmG'CkG
Cuts at: 0 55 171 699 717
Size: 55 116 528 18
Fragments arranged by size:
528 116 55 18
NaeI GCC'GGC
Cuts at: 0 433 484 717
Size: 433 51 233
Fragments arranged by size:
433 233 51
NarI GG'CG_CC
Cuts at: 0 124 132 717
Size: 124 8 585
Fragments arranged by size:
585 124 8
NgoMIV G'CCGG_C
Cuts at: 0 431 482 717
Size: 431 51 235
Fragments arranged by size:
431 235 51
NotI GC'GGCC_GC
Cuts at: 0 183 717
Size: 183 534
PasI CC'CwG_GG
Cuts at: 0 61 717
Size: 61 656
PflMI CCAn_nnn'nTGG
Cuts at: 0 369 567 717
Size: 369 198 150
Fragments arranged by size:
369 198 150
PspOMI G'GGCC_C
Cuts at: 0 57 425 717
Size: 57 368 292
Fragments arranged by size:
368 292 57
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 199 231 717
Size: 199 32 486
Fragments arranged by size:
486 199 32
PvuII CAG'CTG
Cuts at: 0 171 699 717
Size: 171 528 18
Fragments arranged by size:
528 171 18
SfcI C'TryA_G
Cuts at: 0 216 330 580 717
Size: 216 114 250 137
Fragments arranged by size:
250 216 137 114
SfoI GGC'GCC
Cuts at: 0 125 133 717
Size: 125 8 584
Fragments arranged by size:
584 125 8
SnaBI TAC'GTA
Cuts at: 0 383 717
Size: 383 334
StyI C'CwwG_G
Cuts at: 0 114 717
Size: 114 603
Tth111I GACn'n_nGTC
Cuts at: 0 408 717
Size: 408 309
Enzymes that do cut:
AccI Acc65I AflIII ApaI AvaI BamHI BanI BanII
BbeI BglI BlpI Bme1580I BmrI BpmI BsaI BsaAI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI
BsrFI BssSI BstYI BtgI BtgZI BtsI EaeI EagI
EarI Eco57MI EcoO109I FseI FspI FspAI HaeII HincII
KasI KpnI MscI MspA1I NaeI NarI NgoMIV NotI
PasI PflMI PspOMI PsrI PvuII SfcI SfoI SnaBI
StyI Tth111I
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaBI
BsaXI BseRI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoICRI
EcoNI EcoRI EcoRV FalI Hin4I HindIII HpaI MfeI
MluI MmeI MslI NcoI NdeI NheI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI XbaI XcmI XhoI XmaI
XmnI ZraI