(Linear) (Six-base) MAPSORT of: KIEE1746.seq  Check: 5347  from: 1  to: 750



KIEE1746.orf

 With 182 enzymes: SgfI * 

                        February 19, 2021 20:23  ..

AflIII A'CryG_T

Cuts at:      0     682     750 
   Size:        682      68
 
AhdI GACnn_n'nnGTC

Cuts at:      0     255     750 
   Size:        255     495
 
ApaI G_GGCC'C

Cuts at:      0      30     750 
   Size:         30     720
 
ApoI r'AATT_y

Cuts at:      0     201     208     750 
   Size:        201       7     542

  Fragments arranged by size: 

                 542     201       7
 
BanII G_rGCy'C

Cuts at:      0      30     750 
   Size:         30     720
 
BbvCI CC'TCA_GC

Cuts at:      0      40     750 
   Size:         40     710
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     625     750 
   Size:        625     125
 
BfrBI ATG'CAT

Cuts at:      0     425     750 
   Size:        425     325
 
Bme1580I G_kGCm'C

Cuts at:      0      30     750 
   Size:         30     720
 
Bpu10I CC'TnA_GC

Cuts at:      0      40     750 
   Size:         40     710
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     750 
   Size:        382     368
 
BseYI C'CCAG_C

Cuts at:      0       7     750 
   Size:          7     743
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     750 
   Size:         43     707
 
Bsp1286I G_dGCh'C

Cuts at:      0      30     750 
   Size:         30     720
 
BsrDI GCAATG_nn'

Cuts at:      0     147     499     750 
   Size:        147     352     251

  Fragments arranged by size: 

                 352     251     147
 
BtsI GCAGTG_nn'

Cuts at:      0      60     750 
   Size:         60     690
 
DraI TTT'AAA

Cuts at:      0     152     231     750 
   Size:        152      79     519

  Fragments arranged by size: 

                 519     152      79
 
EarI CTCTTCn'nnn_

Cuts at:      0     374     750 
   Size:        374     376
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27      36     159     750 
   Size:         27       9     123     591

  Fragments arranged by size: 

                 591     123      27       9
 
EcoRI G'AATT_C

Cuts at:      0     208     750 
   Size:        208     542
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     241     273     750 
   Size:        241      32     477

  Fragments arranged by size: 

                 477     241      32
 
MslI CAynn'nnrTG

Cuts at:      0     644     750 
   Size:        644     106
 
MspA1I CmG'CkG

Cuts at:      0      80     626     750 
   Size:         80     546     124

  Fragments arranged by size: 

                 546     124      80
 
NsiI A_TGCA'T

Cuts at:      0     427     750 
   Size:        427     323
 
NspI r_CATG'y

Cuts at:      0     686     750 
   Size:        686      64
 
PasI CC'CwG_GG

Cuts at:      0      30     750 
   Size:         30     720
 
PciI A'CATG_T

Cuts at:      0     682     750 
   Size:        682      68
 
PpuMI rG'GwC_Cy

Cuts at:      0      36     159     750 
   Size:         36     123     591

  Fragments arranged by size: 

                 591     123      36
 
PsiI TTA'TAA

Cuts at:      0     463     750 
   Size:        463     287
 
PspOMI G'GGCC_C

Cuts at:      0      26     750 
   Size:         26     724
 
PvuII CAG'CTG

Cuts at:      0      80     750 
   Size:         80     670
 
SmlI C'TyrA_G

Cuts at:      0     361     750 
   Size:        361     389
 
SspI AAT'ATT

Cuts at:      0     327     750 
   Size:        327     423
 
TatI w'GTAC_w

Cuts at:      0     467     559     750 
   Size:        467      92     191

  Fragments arranged by size: 

                 467     191      92
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      97     750 
   Size:         97     653
 

 Enzymes that do cut:

   AflIII     AhdI     ApaI     ApoI    BanII    BbvCI    BciVI    BfrBI
 Bme1580I   Bpu10I    BpuEI    BseYI     BsgI Bsp1286I    BsrDI     BtsI
     DraI     EarI EcoO109I    EcoRI    Hin4I     MslI   MspA1I     NsiI
     NspI     PasI     PciI    PpuMI     PsiI   PspOMI    PvuII     SmlI
     SspI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
     BcgI     BclI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI