Restriction map for
pF1KE1747
(Linear) (Six-base) MAPSORT of: KIEE1747.seq Check: 3345 from: 1 to: 753
KIEE1747.orf
With 182 enzymes: SgfI *
June 2, 2011 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 164 753
Size: 164 589
AatII G_ACGT'C
Cuts at: 0 377 753
Size: 377 376
AccI GT'mk_AC
Cuts at: 0 377 753
Size: 377 376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 753
Size: 598 155
AlwNI CAG_nnn'CTG
Cuts at: 0 23 47 357 471 753
Size: 23 24 310 114 282
Fragments arranged by size:
310 282 114 24 23
ApaI G_GGCC'C
Cuts at: 0 12 591 721 753
Size: 12 579 130 32
Fragments arranged by size:
579 130 32 12
AvaI C'yCGr_G
Cuts at: 0 584 630 753
Size: 584 46 123
Fragments arranged by size:
584 123 46
BanII G_rGCy'C
Cuts at: 0 12 591 629 721 753
Size: 12 579 38 92 32
Fragments arranged by size:
579 92 38 32 12
BclI T'GATC_A
Cuts at: 0 221 753
Size: 221 532
BglI GCCn_nnn'nGGC
Cuts at: 0 725 753
Size: 725 28
Bme1580I G_kGCm'C
Cuts at: 0 12 216 591 721 753
Size: 12 204 375 130 32
Fragments arranged by size:
375 204 130 32 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 459 753
Size: 459 294
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 567 753
Size: 567 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 753
Size: 84 669
BsaHI Gr'CG_yC
Cuts at: 0 374 753
Size: 374 379
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 611 641 753
Size: 611 30 112
Fragments arranged by size:
611 112 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 377 753
Size: 377 376
BseYI C'CCAG_C
Cuts at: 0 104 706 753
Size: 104 602 47
Fragments arranged by size:
602 104 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 753
Size: 60 693
BsiEI CG_ry'CG
Cuts at: 0 690 753
Size: 690 63
BsiHKAI G_wGCw'C
Cuts at: 0 629 753
Size: 629 124
Bsp1286I G_dGCh'C
Cuts at: 0 12 216 591 629 721 753
Size: 12 204 375 38 92 32
Fragments arranged by size:
375 204 92 38 32 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 329 753
Size: 164 165 424
Fragments arranged by size:
424 165 164
BsrBI CCG'CTC
Cuts at: 0 558 753
Size: 558 195
BsrGI T'GTAC_A
Cuts at: 0 125 753
Size: 125 628
BstAPI GCAn_nnn'nTGC
Cuts at: 0 47 753
Size: 47 706
BstXI CCAn_nnnn'nTGG
Cuts at: 0 172 184 753
Size: 172 12 569
Fragments arranged by size:
569 172 12
BstYI r'GATC_y
Cuts at: 0 161 488 753
Size: 161 327 265
Fragments arranged by size:
327 265 161
Bsu36I CC'TnA_GG
Cuts at: 0 16 753
Size: 16 737
BtgI C'CryG_G
Cuts at: 0 520 753
Size: 520 233
BtsI GCAGTG_nn'
Cuts at: 0 88 753
Size: 88 665
EaeI y'GGCC_r
Cuts at: 0 174 656 753
Size: 174 482 97
Fragments arranged by size:
482 174 97
EarI CTCTTCn'nnn_
Cuts at: 0 418 753
Size: 418 335
EcoICRI GAG'CTC
Cuts at: 0 627 753
Size: 627 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 598 753
Size: 84 514 155
Fragments arranged by size:
514 155 84
EcoO109I rG'GnC_Cy
Cuts at: 0 8 561 753
Size: 8 553 192
Fragments arranged by size:
553 192 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 447 753
Size: 447 306
HaeII r_GCGC'y
Cuts at: 0 542 638 753
Size: 542 96 115
Fragments arranged by size:
542 115 96
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 753
Size: 369 384
MscI TGG'CCA
Cuts at: 0 176 658 753
Size: 176 482 95
Fragments arranged by size:
482 176 95
MslI CAynn'nnrTG
Cuts at: 0 170 182 753
Size: 170 12 571
Fragments arranged by size:
571 170 12
MspA1I CmG'CkG
Cuts at: 0 104 753
Size: 104 649
PflMI CCAn_nnn'nTGG
Cuts at: 0 357 753
Size: 357 396
PfoI T'CCnGG_A
Cuts at: 0 475 753
Size: 475 278
PpuMI rG'GwC_Cy
Cuts at: 0 561 753
Size: 561 192
PspOMI G'GGCC_C
Cuts at: 0 8 587 717 753
Size: 8 579 130 36
Fragments arranged by size:
579 130 36 8
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 451 483 753
Size: 451 32 270
Fragments arranged by size:
451 270 32
PstI C_TGCA'G
Cuts at: 0 52 161 340 753
Size: 52 109 179 413
Fragments arranged by size:
413 179 109 52
PvuII CAG'CTG
Cuts at: 0 104 753
Size: 104 649
SacI G_AGCT'C
Cuts at: 0 629 753
Size: 629 124
SanDI GG'GwC_CC
Cuts at: 0 561 753
Size: 561 192
SfcI C'TryA_G
Cuts at: 0 48 157 207 336 753
Size: 48 109 50 129 417
Fragments arranged by size:
417 129 109 50 48
SmaI CCC'GGG
Cuts at: 0 586 753
Size: 586 167
TatI w'GTAC_w
Cuts at: 0 125 753
Size: 125 628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 611 643 753
Size: 611 32 110
Fragments arranged by size:
611 110 32
XmaI C'CCGG_G
Cuts at: 0 584 753
Size: 584 169
ZraI GAC'GTC
Cuts at: 0 375 753
Size: 375 378
Enzymes that do cut:
AarI AatII AccI AcuI AlwNI ApaI AvaI BanII
BclI BglI Bme1580I BmrI BplI BpmI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI HaeII MmeI MscI
MslI MspA1I PflMI PfoI PpuMI PspOMI PsrI PstI
PvuII SacI SanDI SfcI SmaI TatI TstI XmaI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrFI BssHII BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI
XcmI XhoI XmnI