(Linear) (Six-base) MAPSORT of: KIEE1779.seq  Check: 4841  from: 1  to: 864



KIEE1779.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     416     864 
   Size:        376      40     448

  Fragments arranged by size: 

                 448     376      40
 
ApoI r'AATT_y

Cuts at:      0     452     767     864 
   Size:        452     315      97

  Fragments arranged by size: 

                 452     315      97
 
AvaI C'yCGr_G

Cuts at:      0     253     864 
   Size:        253     611
 
BanII G_rGCy'C

Cuts at:      0     590     864 
   Size:        590     274
 
Bme1580I G_kGCm'C

Cuts at:      0     337     864 
   Size:        337     527
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     515     864 
   Size:        515     349
 
BsaAI yAC'GTr

Cuts at:      0     111     864 
   Size:        111     753
 
BsaWI w'CCGG_w

Cuts at:      0     266     864 
   Size:        266     598
 
BsiHKAI G_wGCw'C

Cuts at:      0     590     864 
   Size:        590     274
 
Bsp1286I G_dGCh'C

Cuts at:      0     337     590     864 
   Size:        337     253     274

  Fragments arranged by size: 

                 337     274     253
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     734     864 
   Size:        734     130
 
BstYI r'GATC_y

Cuts at:      0     573     864 
   Size:        573     291
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     228     864 
   Size:        228     636
 
EarI CTCTTCn'nnn_

Cuts at:      0     164     864 
   Size:        164     700
 
EcoICRI GAG'CTC

Cuts at:      0     588     864 
   Size:        588     276
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     416     515     864 
   Size:        376      40      99     349

  Fragments arranged by size: 

                 376     349      99      40
 
EcoRV GAT'ATC

Cuts at:      0     282     864 
   Size:        282     582
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     297     329     864 
   Size:        297      32     535

  Fragments arranged by size: 

                 535     297      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     506     864 
   Size:        481      25     358

  Fragments arranged by size: 

                 481     358      25
 
MslI CAynn'nnrTG

Cuts at:      0      36     342     864 
   Size:         36     306     522

  Fragments arranged by size: 

                 522     306      36
 
PmlI CAC'GTG

Cuts at:      0     111     864 
   Size:        111     753
 
SacI G_AGCT'C

Cuts at:      0     590     864 
   Size:        590     274
 
SapI GCTCTTCn'nnn_

Cuts at:      0     164     864 
   Size:        164     700
 
SspI AAT'ATT

Cuts at:      0     468     864 
   Size:        468     396
 
StyI C'CwwG_G

Cuts at:      0     116     864 
   Size:        116     748
 
TatI w'GTAC_w

Cuts at:      0     257     864 
   Size:        257     607
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     758     790     864 
   Size:        758      32      74

  Fragments arranged by size: 

                 758      74      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     306     864 
   Size:        306     558
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI    BanII Bme1580I     BpmI    BsaAI    BsaWI
  BsiHKAI Bsp1286I    BspMI    BstYI     DrdI     EarI  EcoICRI  Eco57MI
    EcoRV    Hin4I     MmeI     MslI     PmlI     SacI     SapI     SspI
     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EaeI
     EagI     EciI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI