Restriction map for
pF1KE1780
(Linear) (Six-base) MAPSORT of: KIEE1780.seq Check: 5262 from: 1 to: 864
KIEE1780.orf
With 182 enzymes: SgfI *
June 10, 2016 16:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 166 864
Size: 166 698
Acc65I G'GTAC_C
Cuts at: 0 200 864
Size: 200 664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 864
Size: 646 218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 559 864
Size: 559 305
AlwNI CAG_nnn'CTG
Cuts at: 0 92 624 828 864
Size: 92 532 204 36
Fragments arranged by size:
532 204 92 36
ApaI G_GGCC'C
Cuts at: 0 339 864
Size: 339 525
AvaI C'yCGr_G
Cuts at: 0 247 578 864
Size: 247 331 286
Fragments arranged by size:
331 286 247
BanI G'GyrC_C
Cuts at: 0 15 200 355 772 864
Size: 15 185 155 417 92
Fragments arranged by size:
417 185 155 92 15
BanII G_rGCy'C
Cuts at: 0 214 339 425 710 799 864
Size: 214 125 86 285 89 65
Fragments arranged by size:
285 214 125 89 86 65
BbeI G_GCGC'C
Cuts at: 0 19 864
Size: 19 845
BbsI GAAGACnn'nnnn_
Cuts at: 0 703 864
Size: 703 161
BbvCI CC'TCA_GC
Cuts at: 0 639 864
Size: 639 225
BglI GCCn_nnn'nGGC
Cuts at: 0 266 306 391 533 831 864
Size: 266 40 85 142 298 33
Fragments arranged by size:
298 266 142 85 40 33
BlpI GC'TnA_GC
Cuts at: 0 798 864
Size: 798 66
Bme1580I G_kGCm'C
Cuts at: 0 339 455 719 775 822 864
Size: 339 116 264 56 47 42
Fragments arranged by size:
339 264 116 56 47 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 58 864
Size: 58 806
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 654 864
Size: 654 210
Bpu10I CC'TnA_GC
Cuts at: 0 424 639 728 864
Size: 424 215 89 136
Fragments arranged by size:
424 215 136 89
BsaHI Gr'CG_yC
Cuts at: 0 16 864
Size: 16 848
BseYI C'CCAG_C
Cuts at: 0 114 214 466 531 821 864
Size: 114 100 252 65 290 43
Fragments arranged by size:
290 252 114 100 65 43
BsiHKAI G_wGCw'C
Cuts at: 0 425 864
Size: 425 439
Bsp1286I G_dGCh'C
Cuts at: 0 214 339 425 455 710 719 775 799
Size: 214 125 86 30 255 9 56 24
Cuts at: 799 822 864
Size: 23 42
Fragments arranged by size:
255 214 125 86 56 42 30 24
23 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 166 864
Size: 166 698
BsrDI GCAATG_nn'
Cuts at: 0 351 864
Size: 351 513
BsrFI r'CCGG_y
Cuts at: 0 487 613 864
Size: 487 126 251
Fragments arranged by size:
487 251 126
BssSI C'ACGA_G
Cuts at: 0 124 587 864
Size: 124 463 277
Fragments arranged by size:
463 277 124
BstEII G'GTnAC_C
Cuts at: 0 129 864
Size: 129 735
BstXI CCAn_nnnn'nTGG
Cuts at: 0 32 381 864
Size: 32 349 483
Fragments arranged by size:
483 349 32
BstYI r'GATC_y
Cuts at: 0 5 864
Size: 5 859
Bsu36I CC'TnA_GG
Cuts at: 0 836 864
Size: 836 28
BtsI GCAGTG_nn'
Cuts at: 0 846 864
Size: 846 18
EaeI y'GGCC_r
Cuts at: 0 22 58 236 267 489 563 615 864
Size: 22 36 178 31 222 74 52 249
Fragments arranged by size:
249 222 178 74 52 36 31 22
EcoICRI GAG'CTC
Cuts at: 0 423 864
Size: 423 441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 864
Size: 646 218
EcoNI CCTnn'n_nnAGG
Cuts at: 0 477 628 864
Size: 477 151 236
Fragments arranged by size:
477 236 151
EcoO109I rG'GnC_Cy
Cuts at: 0 89 335 558 633 864
Size: 89 246 223 75 231
Fragments arranged by size:
246 231 223 89 75
HaeII r_GCGC'y
Cuts at: 0 19 864
Size: 19 845
KasI G'GCGC_C
Cuts at: 0 15 864
Size: 15 849
KpnI G_GTAC'C
Cuts at: 0 204 864
Size: 204 660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 864
Size: 194 670
MscI TGG'CCA
Cuts at: 0 24 864
Size: 24 840
MslI CAynn'nnrTG
Cuts at: 0 325 864
Size: 325 539
MspA1I CmG'CkG
Cuts at: 0 353 802 864
Size: 353 449 62
Fragments arranged by size:
449 353 62
NaeI GCC'GGC
Cuts at: 0 489 864
Size: 489 375
NarI GG'CG_CC
Cuts at: 0 16 864
Size: 16 848
NgoMIV G'CCGG_C
Cuts at: 0 487 864
Size: 487 377
PflMI CCAn_nnn'nTGG
Cuts at: 0 504 864
Size: 504 360
PpuMI rG'GwC_Cy
Cuts at: 0 558 633 864
Size: 558 75 231
Fragments arranged by size:
558 231 75
PspOMI G'GGCC_C
Cuts at: 0 335 864
Size: 335 529
PstI C_TGCA'G
Cuts at: 0 163 864
Size: 163 701
PvuII CAG'CTG
Cuts at: 0 802 864
Size: 802 62
SacI G_AGCT'C
Cuts at: 0 425 864
Size: 425 439
SfcI C'TryA_G
Cuts at: 0 159 864
Size: 159 705
SfoI GGC'GCC
Cuts at: 0 17 864
Size: 17 847
SmaI CCC'GGG
Cuts at: 0 580 864
Size: 580 284
StuI AGG'CCT
Cuts at: 0 834 864
Size: 834 30
StyI C'CwwG_G
Cuts at: 0 389 864
Size: 389 475
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 492 864
Size: 148 344 372
Fragments arranged by size:
372 344 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 670 702 864
Size: 670 32 162
Fragments arranged by size:
670 162 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 17 89 864
Size: 17 72 775
Fragments arranged by size:
775 72 17
XmaI C'CCGG_G
Cuts at: 0 578 864
Size: 578 286
Enzymes that do cut:
AarI Acc65I AcuI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmrI
BplI Bpu10I BsaHI BseYI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BssSI BstEII BstXI BstYI Bsu36I BtsI EaeI
EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV PflMI PpuMI
PspOMI PstI PvuII SacI SfcI SfoI SmaI StuI
StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BglII BmgBI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BstAPI BstBI BstZ17I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XhoI XmnI ZraI