(Linear) (Six-base) MAPSORT of: KIEE1824.seq  Check: 3251  from: 1  to: 1023



KIEE1824.orf

 With 182 enzymes: SgfI * 

                        June 20, 2019 10:05  ..

Acc65I G'GTAC_C

Cuts at:      0     537    1023 
   Size:        537     486
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     586    1023 
   Size:        586     437
 
AflIII A'CryG_T

Cuts at:      0     164     453    1023 
   Size:        164     289     570

  Fragments arranged by size: 

                 570     289     164
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     221     253    1023 
   Size:        221      32     770

  Fragments arranged by size: 

                 770     221      32
 
ApaI G_GGCC'C

Cuts at:      0     836    1023 
   Size:        836     187
 
AvaI C'yCGr_G

Cuts at:      0     121    1023 
   Size:        121     902
 
BanI G'GyrC_C

Cuts at:      0     537     604     882    1023 
   Size:        537      67     278     141

  Fragments arranged by size: 

                 537     278     141      67
 
BanII G_rGCy'C

Cuts at:      0     836    1023 
   Size:        836     187
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     563    1023 
   Size:        563     460
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     917    1023 
   Size:        917     106
 
BclI T'GATC_A

Cuts at:      0     761    1023 
   Size:        761     262
 
Bme1580I G_kGCm'C

Cuts at:      0     836    1023 
   Size:        836     187
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     698    1023 
   Size:        698     325
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     812    1023 
   Size:        812     211
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     922    1023 
   Size:        922     101
 
BsaWI w'CCGG_w

Cuts at:      0     349    1023 
   Size:        349     674
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     418     791    1023 
   Size:        418     373     232

  Fragments arranged by size: 

                 418     373     232
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227    1023 
   Size:        227     796
 
Bsp1286I G_dGCh'C

Cuts at:      0     836    1023 
   Size:        836     187
 
BspHI T'CATG_A

Cuts at:      0      80    1023 
   Size:         80     943
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     549    1023 
   Size:        549     474
 
BsrBI CCG'CTC

Cuts at:      0     126    1023 
   Size:        126     897
 
BsrFI r'CCGG_y

Cuts at:      0     699    1015    1023 
   Size:        699     316       8

  Fragments arranged by size: 

                 699     316       8
 
BsrGI T'GTAC_A

Cuts at:      0     167    1023 
   Size:        167     856
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     966    1023 
   Size:        966      57
 
BstBI TT'CG_AA

Cuts at:      0     954    1023 
   Size:        954      69
 
BstYI r'GATC_y

Cuts at:      0     107    1023 
   Size:        107     916
 
EarI CTCTTCn'nnn_

Cuts at:      0     499    1023 
   Size:        499     524
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     586     812    1023 
   Size:        586     226     211

  Fragments arranged by size: 

                 586     226     211
 
EcoO109I rG'GnC_Cy

Cuts at:      0     229     832    1023 
   Size:        229     603     191

  Fragments arranged by size: 

                 603     229     191
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     271    1023 
   Size:        271     752
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     344     376     479     511    1023 
   Size:        344      32     103      32     512

  Fragments arranged by size: 

                 512     344     103      32      32
 
KpnI G_GTAC'C

Cuts at:      0     541    1023 
   Size:        541     482
 
MfeI C'AATT_G

Cuts at:      0     426    1023 
   Size:        426     597
 
MslI CAynn'nnrTG

Cuts at:      0     440    1023 
   Size:        440     583
 
MspA1I CmG'CkG

Cuts at:      0      60     579     715     739     781    1023 
   Size:         60     519     136      24      42     242

  Fragments arranged by size: 

                 519     242     136      60      42      24
 
NspI r_CATG'y

Cuts at:      0     168     457    1023 
   Size:        168     289     566

  Fragments arranged by size: 

                 566     289     168
 
PciI A'CATG_T

Cuts at:      0     164     453    1023 
   Size:        164     289     570

  Fragments arranged by size: 

                 570     289     164
 
PpuMI rG'GwC_Cy

Cuts at:      0     229    1023 
   Size:        229     794
 
PshAI GACnn'nnGTC

Cuts at:      0     781    1023 
   Size:        781     242
 
PspOMI G'GGCC_C

Cuts at:      0     832    1023 
   Size:        832     191
 
PspXI vC'TCGA_Gb

Cuts at:      0     121    1023 
   Size:        121     902
 
PstI C_TGCA'G

Cuts at:      0     546    1023 
   Size:        546     477
 
PvuII CAG'CTG

Cuts at:      0      60     579     715     739    1023 
   Size:         60     519     136      24     284

  Fragments arranged by size: 

                 519     284     136      60      24
 
SfcI C'TryA_G

Cuts at:      0     542     609    1023 
   Size:        542      67     414

  Fragments arranged by size: 

                 542     414      67
 
SmlI C'TyrA_G

Cuts at:      0     121     901    1023 
   Size:        121     780     122

  Fragments arranged by size: 

                 780     122     121
 
StyI C'CwwG_G

Cuts at:      0     306     676    1023 
   Size:        306     370     347

  Fragments arranged by size: 

                 370     347     306
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     821    1019    1023 
   Size:        821     198       4

  Fragments arranged by size: 

                 821     198       4
 
TatI w'GTAC_w

Cuts at:      0     167     190     456    1023 
   Size:        167      23     266     567

  Fragments arranged by size: 

                 567     266     167      23
 
XhoI C'TCGA_G

Cuts at:      0     121    1023 
   Size:        121     902
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AloI     ApaI     AvaI     BanI    BanII
     BbsI    BciVI     BclI Bme1580I     BmrI     BpmI    BpuEI    BsaWI
    BseRI     BsgI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BstAPI    BstBI    BstYI     EarI  Eco57MI EcoO109I     FalI    Hin4I
     KpnI     MfeI     MslI   MspA1I     NspI     PciI    PpuMI    PshAI
   PspOMI    PspXI     PstI    PvuII     SfcI     SmlI     StyI    TaqII
     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BsrDI   BssHII    BssSI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI     PsiI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI