Restriction map for
pF1KE1843
(Linear) (Six-base) MAPSORT of: KIEE1843.seq Check: 9129 from: 1 to: 1074
KIEE1843.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 509 1074
Size: 509 565
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1074
Size: 71 1003
AfeI AGC'GCT
Cuts at: 0 93 1074
Size: 93 981
AflIII A'CryG_T
Cuts at: 0 983 1074
Size: 983 91
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 923 955 1074
Size: 923 32 119
Fragments arranged by size:
923 119 32
AlwNI CAG_nnn'CTG
Cuts at: 0 599 1074
Size: 599 475
BanII G_rGCy'C
Cuts at: 0 306 640 1074
Size: 306 334 434
Fragments arranged by size:
434 334 306
BbsI GAAGACnn'nnnn_
Cuts at: 0 293 1074
Size: 293 781
BbvCI CC'TCA_GC
Cuts at: 0 88 1074
Size: 88 986
BciVI GTATCCnnnnn_n'
Cuts at: 0 328 1074
Size: 328 746
BmrI ACTGGGnnnn_n'
Cuts at: 0 609 729 1074
Size: 609 120 345
Fragments arranged by size:
609 345 120
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 878 1074
Size: 878 196
Bpu10I CC'TnA_GC
Cuts at: 0 88 1074
Size: 88 986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 1074
Size: 290 784
BsaI GGTCTCn'nnnn_
Cuts at: 0 107 418 1074
Size: 107 311 656
Fragments arranged by size:
656 311 107
BsaBI GATnn'nnATC
Cuts at: 0 209 1074
Size: 209 865
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 682 802 1074
Size: 37 645 120 272
Fragments arranged by size:
645 272 120 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 759 1074
Size: 759 315
BsiHKAI G_wGCw'C
Cuts at: 0 306 640 1074
Size: 306 334 434
Fragments arranged by size:
434 334 306
Bsp1286I G_dGCh'C
Cuts at: 0 306 640 1074
Size: 306 334 434
Fragments arranged by size:
434 334 306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 509 1074
Size: 509 565
BstEII G'GTnAC_C
Cuts at: 0 916 949 1074
Size: 916 33 125
Fragments arranged by size:
916 125 33
BstYI r'GATC_y
Cuts at: 0 26 630 1074
Size: 26 604 444
Fragments arranged by size:
604 444 26
Bsu36I CC'TnA_GG
Cuts at: 0 20 1074
Size: 20 1054
BtgI C'CryG_G
Cuts at: 0 912 1074
Size: 912 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 405 1074
Size: 405 669
BtsI GCAGTG_nn'
Cuts at: 0 1049 1074
Size: 1049 25
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1038 1073 1074
Size: 1038 35 1
Fragments arranged by size:
1038 35 1
EarI CTCTTCn'nnn_
Cuts at: 0 47 421 433 501 653 680 824 839
Size: 47 374 12 68 152 27 144 15
Cuts at: 839 1074
Size: 235
Fragments arranged by size:
374 235 152 144 68 47 27 15
12
EcoICRI GAG'CTC
Cuts at: 0 304 638 1074
Size: 304 334 436
Fragments arranged by size:
436 334 304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1074
Size: 71 1003
EcoNI CCTnn'n_nnAGG
Cuts at: 0 19 469 1074
Size: 19 450 605
Fragments arranged by size:
605 450 19
EcoO109I rG'GnC_Cy
Cuts at: 0 458 897 1074
Size: 458 439 177
Fragments arranged by size:
458 439 177
HaeII r_GCGC'y
Cuts at: 0 95 1074
Size: 95 979
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 734 766 919 951 1074
Size: 622 32 80 32 153 32 123
Fragments arranged by size:
622 153 123 80 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 966 1074
Size: 966 108
MspA1I CmG'CkG
Cuts at: 0 218 1074
Size: 218 856
NcoI C'CATG_G
Cuts at: 0 912 1074
Size: 912 162
NdeI CA'TA_TG
Cuts at: 0 654 693 1074
Size: 654 39 381
Fragments arranged by size:
654 381 39
NspI r_CATG'y
Cuts at: 0 987 1074
Size: 987 87
PciI A'CATG_T
Cuts at: 0 983 1074
Size: 983 91
PflMI CCAn_nnn'nTGG
Cuts at: 0 599 727 1074
Size: 599 128 347
Fragments arranged by size:
599 347 128
PfoI T'CCnGG_A
Cuts at: 0 38 1074
Size: 38 1036
PpuMI rG'GwC_Cy
Cuts at: 0 458 1074
Size: 458 616
SacI G_AGCT'C
Cuts at: 0 306 640 1074
Size: 306 334 434
Fragments arranged by size:
434 334 306
SapI GCTCTTCn'nnn_
Cuts at: 0 433 824 1074
Size: 433 391 250
Fragments arranged by size:
433 391 250
SfcI C'TryA_G
Cuts at: 0 782 1074
Size: 782 292
SmlI C'TyrA_G
Cuts at: 0 305 1074
Size: 305 769
SpeI A'CTAG_T
Cuts at: 0 577 1074
Size: 577 497
StyI C'CwwG_G
Cuts at: 0 174 912 1074
Size: 174 738 162
Fragments arranged by size:
738 174 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 591 987 1074
Size: 591 396 87
Fragments arranged by size:
591 396 87
XbaI T'CTAG_A
Cuts at: 0 840 1074
Size: 840 234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 600 1074
Size: 600 474
XmnI GAAnn'nnTTC
Cuts at: 0 56 510 1074
Size: 56 454 564
Fragments arranged by size:
564 454 56
Enzymes that do cut:
AarI AcuI AfeI AflIII AloI AlwNI BanII BbsI
BbvCI BciVI BmrI BplI Bpu10I BpuEI BsaI BsaBI
BseRI BsgI BsiHKAI Bsp1286I BspMI BstEII BstYI Bsu36I
BtgI BtgZI BtsI CspCI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I MmeI MspA1I NcoI NdeI NspI
PciI PflMI PfoI PpuMI SacI SapI SfcI SmlI
SpeI StyI TaqII XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI BpmI BsaAI
BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstZ17I ClaI DraI DraIII DrdI
EaeI EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XhoI XmaI ZraI