Restriction map for
pF1KE1864
(Linear) (Six-base) MAPSORT of: KIEE1864.seq Check: 254 from: 1 to: 1155
KIEE1864.orf
With 182 enzymes: SgfI *
February 7, 2012 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 850 1155
Size: 850 305
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 1155
Size: 892 263
AhdI GACnn_n'nnGTC
Cuts at: 0 716 1155
Size: 716 439
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 820 852 1155
Size: 820 32 303
Fragments arranged by size:
820 303 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1050 1113 1155
Size: 1050 63 42
Fragments arranged by size:
1050 63 42
ApaI G_GGCC'C
Cuts at: 0 13 1155
Size: 13 1142
ApoI r'AATT_y
Cuts at: 0 498 1155
Size: 498 657
BanI G'GyrC_C
Cuts at: 0 1150 1155
Size: 1150 5
BanII G_rGCy'C
Cuts at: 0 13 74 388 416 474 1034 1155
Size: 13 61 314 28 58 560 121
Fragments arranged by size:
560 314 121 61 58 28 13
BbeI G_GCGC'C
Cuts at: 0 1154 1155
Size: 1154 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 9 73 374 529 1155
Size: 9 64 301 155 626
Fragments arranged by size:
626 301 155 64 9
BbvCI CC'TCA_GC
Cuts at: 0 140 1155
Size: 140 1015
BfrBI ATG'CAT
Cuts at: 0 549 1155
Size: 549 606
BlpI GC'TnA_GC
Cuts at: 0 68 1155
Size: 68 1087
Bme1580I G_kGCm'C
Cuts at: 0 13 458 617 887 1155
Size: 13 445 159 270 268
Fragments arranged by size:
445 270 268 159 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 453 1057 1155
Size: 453 604 98
Fragments arranged by size:
604 453 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 450 1155
Size: 322 128 705
Fragments arranged by size:
705 322 128
Bpu10I CC'TnA_GC
Cuts at: 0 140 1155
Size: 140 1015
BsaI GGTCTCn'nnnn_
Cuts at: 0 247 1155
Size: 247 908
BsaHI Gr'CG_yC
Cuts at: 0 717 1151 1155
Size: 717 434 4
Fragments arranged by size:
717 434 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 820 850 1155
Size: 820 30 305
Fragments arranged by size:
820 305 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 618 841 1155
Size: 618 223 314
Fragments arranged by size:
618 314 223
BseYI C'CCAG_C
Cuts at: 0 6 389 749 1155
Size: 6 383 360 406
Fragments arranged by size:
406 383 360 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1155
Size: 142 1013
BsiHKAI G_wGCw'C
Cuts at: 0 388 416 474 1034 1155
Size: 388 28 58 560 121
Fragments arranged by size:
560 388 121 58 28
BsmI GAATG_Cn'
Cuts at: 0 330 1155
Size: 330 825
Bsp1286I G_dGCh'C
Cuts at: 0 13 74 388 416 458 474 617 887
Size: 13 61 314 28 42 16 143 270
Cuts at: 887 1034 1155
Size: 147 121
Fragments arranged by size:
314 270 147 143 121 61 42 28
16 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 850 1035 1155
Size: 850 185 120
Fragments arranged by size:
850 185 120
BssSI C'ACGA_G
Cuts at: 0 1033 1155
Size: 1033 122
BstYI r'GATC_y
Cuts at: 0 115 359 1155
Size: 115 244 796
Fragments arranged by size:
796 244 115
Bsu36I CC'TnA_GG
Cuts at: 0 286 601 1155
Size: 286 315 554
Fragments arranged by size:
554 315 286
BtgI C'CryG_G
Cuts at: 0 620 1155
Size: 620 535
BtsI GCAGTG_nn'
Cuts at: 0 760 1155
Size: 760 395
DraIII CAC_nnn'GTG
Cuts at: 0 882 1155
Size: 882 273
EaeI y'GGCC_r
Cuts at: 0 482 803 1155
Size: 482 321 352
Fragments arranged by size:
482 352 321
EarI CTCTTCn'nnn_
Cuts at: 0 421 603 1155
Size: 421 182 552
Fragments arranged by size:
552 421 182
EcoICRI GAG'CTC
Cuts at: 0 386 414 472 1032 1155
Size: 386 28 58 560 123
Fragments arranged by size:
560 386 123 58 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 450 892 1155
Size: 322 128 442 263
Fragments arranged by size:
442 322 263 128
EcoO109I rG'GnC_Cy
Cuts at: 0 282 1047 1155
Size: 282 765 108
Fragments arranged by size:
765 282 108
EcoRV GAT'ATC
Cuts at: 0 580 1155
Size: 580 575
HaeII r_GCGC'y
Cuts at: 0 1154 1155
Size: 1154 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 285 317 522 554 702 715 734 747
Size: 285 32 205 32 148 13 19 13
Cuts at: 747 1107 1139 1155
Size: 360 32 16
Fragments arranged by size:
360 285 205 148 32 32 32 19
16 13 13
KasI G'GCGC_C
Cuts at: 0 1150 1155
Size: 1150 5
MscI TGG'CCA
Cuts at: 0 484 805 1155
Size: 484 321 350
Fragments arranged by size:
484 350 321
MslI CAynn'nnrTG
Cuts at: 0 152 664 1085 1155
Size: 152 512 421 70
Fragments arranged by size:
512 421 152 70
MspA1I CmG'CkG
Cuts at: 0 860 1015 1155
Size: 860 155 140
Fragments arranged by size:
860 155 140
NarI GG'CG_CC
Cuts at: 0 1151 1155
Size: 1151 4
NcoI C'CATG_G
Cuts at: 0 620 1155
Size: 620 535
NsiI A_TGCA'T
Cuts at: 0 551 1155
Size: 551 604
NspI r_CATG'y
Cuts at: 0 553 674 1155
Size: 553 121 481
Fragments arranged by size:
553 481 121
PasI CC'CwG_GG
Cuts at: 0 204 1155
Size: 204 951
PflMI CCAn_nnn'nTGG
Cuts at: 0 432 1155
Size: 432 723
PfoI T'CCnGG_A
Cuts at: 0 54 338 1049 1155
Size: 54 284 711 106
Fragments arranged by size:
711 284 106 54
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 820 852 1155
Size: 820 32 303
Fragments arranged by size:
820 303 32
PpuMI rG'GwC_Cy
Cuts at: 0 282 1047 1155
Size: 282 765 108
Fragments arranged by size:
765 282 108
PsiI TTA'TAA
Cuts at: 0 89 1155
Size: 89 1066
PspOMI G'GGCC_C
Cuts at: 0 9 1155
Size: 9 1146
PstI C_TGCA'G
Cuts at: 0 566 1020 1046 1155
Size: 566 454 26 109
Fragments arranged by size:
566 454 109 26
PvuII CAG'CTG
Cuts at: 0 1015 1155
Size: 1015 140
SacI G_AGCT'C
Cuts at: 0 388 416 474 1034 1155
Size: 388 28 58 560 121
Fragments arranged by size:
560 388 121 58 28
SapI GCTCTTCn'nnn_
Cuts at: 0 421 1155
Size: 421 734
SbfI CC_TGCA'GG
Cuts at: 0 566 1046 1155
Size: 566 480 109
Fragments arranged by size:
566 480 109
SfcI C'TryA_G
Cuts at: 0 562 1016 1042 1155
Size: 562 454 26 113
Fragments arranged by size:
562 454 113 26
SfoI GGC'GCC
Cuts at: 0 1152 1155
Size: 1152 3
SphI G_CATG'C
Cuts at: 0 553 1155
Size: 553 602
StuI AGG'CCT
Cuts at: 0 646 1155
Size: 646 509
StyI C'CwwG_G
Cuts at: 0 479 620 819 1155
Size: 479 141 199 336
Fragments arranged by size:
479 336 199 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 763 789 1076 1155
Size: 763 26 287 79
Fragments arranged by size:
763 287 79 26
XbaI T'CTAG_A
Cuts at: 0 582 1155
Size: 582 573
Enzymes that do cut:
AarI AcuI AhdI AloI AlwNI ApaI ApoI BanI
BanII BbeI BbsI BbvCI BfrBI BlpI Bme1580I BmrI
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BssSI BstYI Bsu36I BtgI
BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV
HaeII Hin4I KasI MscI MslI MspA1I NarI NcoI
NsiI NspI PasI PflMI PfoI PpiI PpuMI PsiI
PspOMI PstI PvuII SacI SapI SbfI SfcI SfoI
SphI StuI StyI TaqII XbaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BcgI BciVI BclI BglI BglII BmgBI
BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoNI EcoRI FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NaeI NdeI NgoMIV NheI NotI NruI PacI
PciI PmeI PmlI PshAI PspXI PsrI PvuI RsrII
SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI TatI
TstI Tth111I XcmI XhoI XmaI XmnI ZraI