(Linear) (Six-base) MAPSORT of: KIEE1875.seq Check: 1052 from: 1 to: 1194 KIEE1875.orf With 182 enzymes: SgfI * July 27, 2017 10:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 733 1194 Size: 733 461 AccI GT'mk_AC Cuts at: 0 1159 1194 Size: 1159 35 AlwNI CAG_nnn'CTG Cuts at: 0 512 618 1134 1194 Size: 512 106 516 60 Fragments arranged by size: 516 512 106 60 ApaI G_GGCC'C Cuts at: 0 246 418 1194 Size: 246 172 776 Fragments arranged by size: 776 246 172 AvaI C'yCGr_G Cuts at: 0 285 1194 Size: 285 909 BanI G'GyrC_C Cuts at: 0 265 1194 Size: 265 929 BanII G_rGCy'C Cuts at: 0 230 246 418 458 1194 Size: 230 16 172 40 736 Fragments arranged by size: 736 230 172 40 16 BbeI G_GCGC'C Cuts at: 0 269 1194 Size: 269 925 BglI GCCn_nnn'nGGC Cuts at: 0 290 1194 Size: 290 904 BlpI GC'TnA_GC Cuts at: 0 674 1194 Size: 674 520 Bme1580I G_kGCm'C Cuts at: 0 246 359 418 1194 Size: 246 113 59 776 Fragments arranged by size: 776 246 113 59 BmgBI CAC'GTC Cuts at: 0 744 1194 Size: 744 450 BmrI ACTGGGnnnn_n' Cuts at: 0 298 346 592 1194 Size: 298 48 246 602 Fragments arranged by size: 602 298 246 48 BmtI G_CTAG'C Cuts at: 0 335 1194 Size: 335 859 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 282 472 1194 Size: 282 190 722 Fragments arranged by size: 722 282 190 Bpu10I CC'TnA_GC Cuts at: 0 22 139 1194 Size: 22 117 1055 Fragments arranged by size: 1055 117 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 1014 1194 Size: 556 458 180 Fragments arranged by size: 556 458 180 BsaHI Gr'CG_yC Cuts at: 0 104 266 1194 Size: 104 162 928 Fragments arranged by size: 928 162 104 BsaWI w'CCGG_w Cuts at: 0 230 235 1194 Size: 230 5 959 Fragments arranged by size: 959 230 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 248 261 409 512 670 947 1194 Size: 248 13 148 103 158 277 247 Fragments arranged by size: 277 248 247 158 148 103 13 BseYI C'CCAG_C Cuts at: 0 275 926 1194 Size: 275 651 268 Fragments arranged by size: 651 275 268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 1194 Size: 79 1115 BsiEI CG_ry'CG Cuts at: 0 250 495 1194 Size: 250 245 699 Fragments arranged by size: 699 250 245 BsiHKAI G_wGCw'C Cuts at: 0 230 458 569 1194 Size: 230 228 111 625 Fragments arranged by size: 625 230 228 111 BsmBI CGTCTCn'nnnn_ Cuts at: 0 522 1194 Size: 522 672 Bsp1286I G_dGCh'C Cuts at: 0 230 246 359 418 458 569 1194 Size: 230 16 113 59 40 111 625 Fragments arranged by size: 625 230 113 111 59 40 16 BspEI T'CCGG_A Cuts at: 0 230 1194 Size: 230 964 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 733 1194 Size: 733 461 BsrBI CCG'CTC Cuts at: 0 18 26 1194 Size: 18 8 1168 Fragments arranged by size: 1168 18 8 BsrDI GCAATG_nn' Cuts at: 0 821 1194 Size: 821 373 BsrFI r'CCGG_y Cuts at: 0 557 1194 Size: 557 637 BsrGI T'GTAC_A Cuts at: 0 1140 1194 Size: 1140 54 BssSI C'ACGA_G Cuts at: 0 76 457 1194 Size: 76 381 737 Fragments arranged by size: 737 381 76 BstZ17I GTA'TAC Cuts at: 0 1160 1194 Size: 1160 34 Bsu36I CC'TnA_GG Cuts at: 0 392 1194 Size: 392 802 BtgI C'CryG_G Cuts at: 0 796 1194 Size: 796 398 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 52 1194 Size: 52 1142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 980 1015 1194 Size: 980 35 179 Fragments arranged by size: 980 179 35 DraIII CAC_nnn'GTG Cuts at: 0 531 1194 Size: 531 663 EaeI y'GGCC_r Cuts at: 0 80 129 247 492 533 1194 Size: 80 49 118 245 41 661 Fragments arranged by size: 661 245 118 80 49 41 EagI C'GGCC_G Cuts at: 0 247 492 1194 Size: 247 245 702 Fragments arranged by size: 702 247 245 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1012 1194 Size: 1012 182 EcoICRI GAG'CTC Cuts at: 0 228 456 1194 Size: 228 228 738 Fragments arranged by size: 738 228 228 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 282 472 1194 Size: 282 190 722 Fragments arranged by size: 722 282 190 EcoNI CCTnn'n_nnAGG Cuts at: 0 260 1194 Size: 260 934 EcoO109I rG'GnC_Cy Cuts at: 0 242 415 1194 Size: 242 173 779 Fragments arranged by size: 779 242 173 HaeII r_GCGC'y Cuts at: 0 35 269 612 1194 Size: 35 234 343 582 Fragments arranged by size: 582 343 234 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 502 534 1194 Size: 502 32 660 Fragments arranged by size: 660 502 32 KasI G'GCGC_C Cuts at: 0 265 1194 Size: 265 929 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 141 1194 Size: 141 1053 MscI TGG'CCA Cuts at: 0 535 1194 Size: 535 659 MslI CAynn'nnrTG Cuts at: 0 442 757 1194 Size: 442 315 437 Fragments arranged by size: 442 437 315 MspA1I CmG'CkG Cuts at: 0 60 177 405 618 1194 Size: 60 117 228 213 576 Fragments arranged by size: 576 228 213 117 60 NarI GG'CG_CC Cuts at: 0 266 1194 Size: 266 928 NheI G'CTAG_C Cuts at: 0 331 1194 Size: 331 863 NotI GC'GGCC_GC Cuts at: 0 492 1194 Size: 492 702 NspI r_CATG'y Cuts at: 0 441 978 1194 Size: 441 537 216 Fragments arranged by size: 537 441 216 PasI CC'CwG_GG Cuts at: 0 506 1194 Size: 506 688 PflMI CCAn_nnn'nTGG Cuts at: 0 618 1134 1194 Size: 618 516 60 Fragments arranged by size: 618 516 60 PshAI GACnn'nnGTC Cuts at: 0 346 1194 Size: 346 848 PspOMI G'GGCC_C Cuts at: 0 242 414 1194 Size: 242 172 780 Fragments arranged by size: 780 242 172 PstI C_TGCA'G Cuts at: 0 182 608 1194 Size: 182 426 586 Fragments arranged by size: 586 426 182 PvuII CAG'CTG Cuts at: 0 618 1194 Size: 618 576 RsrII CG'GwC_CG Cuts at: 0 233 1194 Size: 233 961 SacI G_AGCT'C Cuts at: 0 230 458 1194 Size: 230 228 736 Fragments arranged by size: 736 230 228 ScaI AGT'ACT Cuts at: 0 577 1194 Size: 577 617 SexAI A'CCwGG_T Cuts at: 0 1127 1194 Size: 1127 67 SfcI C'TryA_G Cuts at: 0 55 178 604 1194 Size: 55 123 426 590 Fragments arranged by size: 590 426 123 55 SfoI GGC'GCC Cuts at: 0 267 1194 Size: 267 927 SmaI CCC'GGG Cuts at: 0 287 1194 Size: 287 907 SmlI C'TyrA_G Cuts at: 0 571 1029 1194 Size: 571 458 165 Fragments arranged by size: 571 458 165 StyI C'CwwG_G Cuts at: 0 99 1194 Size: 99 1095 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 336 611 1085 1194 Size: 336 275 474 109 Fragments arranged by size: 474 336 275 109 TatI w'GTAC_w Cuts at: 0 575 1140 1194 Size: 575 565 54 Fragments arranged by size: 575 565 54 Tth111I GACn'n_nGTC Cuts at: 0 514 1194 Size: 514 680 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 306 1194 Size: 306 888 XmaI C'CCGG_G Cuts at: 0 285 1194 Size: 285 909 Enzymes that do cut: AarI AccI AlwNI ApaI AvaI BanI BanII BbeI BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstZ17I Bsu36I BtgI BtgZI CspCI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI NheI NotI NspI PasI PflMI PshAI PspOMI PstI PvuII RsrII SacI ScaI SexAI SfcI SfoI SmaI SmlI StyI TaqII TatI Tth111I XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BplI BsaI BsaAI BsaBI BsaXI BsiWI BsmI BspHI BssHII BstAPI BstBI BstEII BstXI BstYI BtsI ClaI DraI DrdI EarI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI SacII SalI SanDI SapI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XhoI XmnI ZraI