Restriction map for
pF1KE1911
(Linear) (Six-base) MAPSORT of: KIEE1911.seq Check: 7435 from: 1 to: 1335
KIEE1911.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 1335
Size: 721 614
Acc65I G'GTAC_C
Cuts at: 0 442 1335
Size: 442 893
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 1335
Size: 618 717
AflIII A'CryG_T
Cuts at: 0 143 494 1335
Size: 143 351 841
Fragments arranged by size:
841 351 143
AhdI GACnn_n'nnGTC
Cuts at: 0 1254 1335
Size: 1254 81
AleI CACnn'nnGTG
Cuts at: 0 535 1335
Size: 535 800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 193 1335
Size: 193 1142
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1288 1320 1335
Size: 1288 32 15
Fragments arranged by size:
1288 32 15
AlwNI CAG_nnn'CTG
Cuts at: 0 1128 1335
Size: 1128 207
ApaI G_GGCC'C
Cuts at: 0 239 1335
Size: 239 1096
ApaLI G'TGCA_C
Cuts at: 0 13 1335
Size: 13 1322
AvaI C'yCGr_G
Cuts at: 0 231 859 1073 1335
Size: 231 628 214 262
Fragments arranged by size:
628 262 231 214
BamHI G'GATC_C
Cuts at: 0 1031 1335
Size: 1031 304
BanI G'GyrC_C
Cuts at: 0 49 100 211 442 507 1335
Size: 49 51 111 231 65 828
Fragments arranged by size:
828 231 111 65 51 49
BanII G_rGCy'C
Cuts at: 0 209 239 545 866 1335
Size: 209 30 306 321 469
Fragments arranged by size:
469 321 306 209 30
BbeI G_GCGC'C
Cuts at: 0 53 1335
Size: 53 1282
BbvCI CC'TCA_GC
Cuts at: 0 1319 1335
Size: 1319 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 61 1335
Size: 27 34 1274
Fragments arranged by size:
1274 34 27
BclI T'GATC_A
Cuts at: 0 68 1335
Size: 68 1267
BglI GCCn_nnn'nGGC
Cuts at: 0 243 1335
Size: 243 1092
BglII A'GATC_T
Cuts at: 0 248 1335
Size: 248 1087
Bme1580I G_kGCm'C
Cuts at: 0 17 103 182 239 314 860 1335
Size: 17 86 79 57 75 546 475
Fragments arranged by size:
546 475 86 79 75 57 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 309 437 1335
Size: 309 128 898
Fragments arranged by size:
898 309 128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1335
Size: 223 1112
Bpu10I CC'TnA_GC
Cuts at: 0 1319 1335
Size: 1319 16
BsaI GGTCTCn'nnnn_
Cuts at: 0 586 1335
Size: 586 749
BsaAI yAC'GTr
Cuts at: 0 177 1335
Size: 177 1158
BsaHI Gr'CG_yC
Cuts at: 0 50 1335
Size: 50 1285
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1288 1318 1335
Size: 1288 30 17
Fragments arranged by size:
1288 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 484 1306 1309 1324 1327 1335
Size: 439 45 822 3 15 3 8
Fragments arranged by size:
822 439 45 15 8 3 3
BseYI C'CCAG_C
Cuts at: 0 289 869 1335
Size: 289 580 466
Fragments arranged by size:
580 466 289
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1168 1335
Size: 772 396 167
Fragments arranged by size:
772 396 167
BsiEI CG_ry'CG
Cuts at: 0 259 1335
Size: 259 1076
BsiHKAI G_wGCw'C
Cuts at: 0 17 83 194 866 1335
Size: 17 66 111 672 469
Fragments arranged by size:
672 469 111 66 17
Bsp1286I G_dGCh'C
Cuts at: 0 17 83 103 182 194 209 239 314
Size: 17 66 20 79 12 15 30 75
Cuts at: 314 545 860 866 1335
Size: 231 315 6 469
Fragments arranged by size:
469 315 231 79 75 66 30 20
17 15 12 6
BspHI T'CATG_A
Cuts at: 0 488 1335
Size: 488 847
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 721 755 1335
Size: 131 590 34 580
Fragments arranged by size:
590 580 131 34
BsrFI r'CCGG_y
Cuts at: 0 163 1335
Size: 163 1172
BssSI C'ACGA_G
Cuts at: 0 193 1335
Size: 193 1142
BstEII G'GTnAC_C
Cuts at: 0 667 705 1335
Size: 667 38 630
Fragments arranged by size:
667 630 38
BstYI r'GATC_y
Cuts at: 0 198 248 461 1031 1335
Size: 198 50 213 570 304
Fragments arranged by size:
570 304 213 198 50
BtgI C'CryG_G
Cuts at: 0 95 108 215 918 1335
Size: 95 13 107 703 417
Fragments arranged by size:
703 417 107 95 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 89 1335
Size: 89 1246
BtsI GCAGTG_nn'
Cuts at: 0 37 1335
Size: 37 1298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1083 1118 1335
Size: 1083 35 217
Fragments arranged by size:
1083 217 35
DraIII CAC_nnn'GTG
Cuts at: 0 684 1335
Size: 684 651
EaeI y'GGCC_r
Cuts at: 0 256 921 1115 1157 1335
Size: 256 665 194 42 178
Fragments arranged by size:
665 256 194 178 42
EagI C'GGCC_G
Cuts at: 0 256 1335
Size: 256 1079
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1080 1335
Size: 1080 255
EcoICRI GAG'CTC
Cuts at: 0 864 1335
Size: 864 471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 618 1335
Size: 223 395 717
Fragments arranged by size:
717 395 223
EcoO109I rG'GnC_Cy
Cuts at: 0 235 236 1335
Size: 235 1 1099
Fragments arranged by size:
1099 235 1
HaeII r_GCGC'y
Cuts at: 0 53 850 1335
Size: 53 797 485
Fragments arranged by size:
797 485 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1240 1272 1335
Size: 1240 32 63
Fragments arranged by size:
1240 63 32
KasI G'GCGC_C
Cuts at: 0 49 1335
Size: 49 1286
KpnI G_GTAC'C
Cuts at: 0 446 1335
Size: 446 889
MslI CAynn'nnrTG
Cuts at: 0 142 535 964 1159 1335
Size: 142 393 429 195 176
Fragments arranged by size:
429 393 195 176 142
MspA1I CmG'CkG
Cuts at: 0 129 402 1335
Size: 129 273 933
Fragments arranged by size:
933 273 129
NarI GG'CG_CC
Cuts at: 0 50 1335
Size: 50 1285
NcoI C'CATG_G
Cuts at: 0 215 918 1335
Size: 215 703 417
Fragments arranged by size:
703 417 215
NspI r_CATG'y
Cuts at: 0 963 1335
Size: 963 372
PasI CC'CwG_GG
Cuts at: 0 414 1335
Size: 414 921
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 414 1335
Size: 59 355 921
Fragments arranged by size:
921 355 59
PfoI T'CCnGG_A
Cuts at: 0 464 1121 1335
Size: 464 657 214
Fragments arranged by size:
657 464 214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1204 1236 1288 1320 1335
Size: 1204 32 52 32 15
Fragments arranged by size:
1204 52 32 32 15
PspOMI G'GGCC_C
Cuts at: 0 235 1335
Size: 235 1100
PstI C_TGCA'G
Cuts at: 0 128 392 1335
Size: 128 264 943
Fragments arranged by size:
943 264 128
PvuII CAG'CTG
Cuts at: 0 129 402 1335
Size: 129 273 933
Fragments arranged by size:
933 273 129
SacI G_AGCT'C
Cuts at: 0 866 1335
Size: 866 469
SbfI CC_TGCA'GG
Cuts at: 0 392 1335
Size: 392 943
SexAI A'CCwGG_T
Cuts at: 0 680 1250 1335
Size: 680 570 85
Fragments arranged by size:
680 570 85
SfcI C'TryA_G
Cuts at: 0 124 388 1335
Size: 124 264 947
Fragments arranged by size:
947 264 124
SfoI GGC'GCC
Cuts at: 0 51 1335
Size: 51 1284
SgrAI Cr'CCGG_yG
Cuts at: 0 163 1335
Size: 163 1172
StuI AGG'CCT
Cuts at: 0 1177 1335
Size: 1177 158
StyI C'CwwG_G
Cuts at: 0 215 305 918 1335
Size: 215 90 613 417
Fragments arranged by size:
613 417 215 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 406 883 1335
Size: 265 141 477 452
Fragments arranged by size:
477 452 265 141
TatI w'GTAC_w
Cuts at: 0 146 1335
Size: 146 1189
Tth111I GACn'n_nGTC
Cuts at: 0 429 1335
Size: 429 906
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 415 894 972 1335
Size: 415 479 78 363
Fragments arranged by size:
479 415 363 78
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BclI BglI BglII Bme1580I BmrI BpmI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrFI BssSI BstEII
BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EagI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI KpnI
MslI MspA1I NarI NcoI NspI PasI PflMI PfoI
PpiI PspOMI PstI PvuII SacI SbfI SexAI SfcI
SfoI SgrAI StuI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI ApoI AscI
AseI AsiSI AvrII BaeI BbsI BciVI BfrBI BlpI
BmgBI BmtI BplI BpuEI BsaBI BsaWI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BsrGI BssHII BstAPI BstBI
BstXI BstZ17I Bsu36I ClaI DraI DrdI EarI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MmeI MscI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI ScaI SfiI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI
XhoI XmaI XmnI ZraI