Restriction map for
pF1KE1962
(Linear) (Six-base) MAPSORT of: KIEE1962.seq Check: 4292 from: 1 to: 1581
KIEE1962.orf
With 182 enzymes: SgfI *
December 11, 2009 15:37 ..
AclI AA'CG_TT
Cuts at: 0 1316 1581
Size: 1316 265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1036 1420 1581
Size: 547 489 384 161
Fragments arranged by size:
547 489 384 161
AleI CACnn'nnGTG
Cuts at: 0 1534 1581
Size: 1534 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 708 1581
Size: 708 873
ApaI G_GGCC'C
Cuts at: 0 137 146 1581
Size: 137 9 1435
Fragments arranged by size:
1435 137 9
ApoI r'AATT_y
Cuts at: 0 203 403 697 1412 1581
Size: 203 200 294 715 169
Fragments arranged by size:
715 294 203 200 169
AvaI C'yCGr_G
Cuts at: 0 1142 1581
Size: 1142 439
BamHI G'GATC_C
Cuts at: 0 15 468 531 1581
Size: 15 453 63 1050
Fragments arranged by size:
1050 453 63 15
BanII G_rGCy'C
Cuts at: 0 137 146 1581
Size: 137 9 1435
Fragments arranged by size:
1435 137 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 322 1581
Size: 322 1259
BbvCI CC'TCA_GC
Cuts at: 0 980 1581
Size: 980 601
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1161 1195 1581
Size: 1161 34 386
Fragments arranged by size:
1161 386 34
BglI GCCn_nnn'nGGC
Cuts at: 0 141 1581
Size: 141 1440
Bme1580I G_kGCm'C
Cuts at: 0 137 146 1581
Size: 137 9 1435
Fragments arranged by size:
1435 137 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 94 435 928 1581
Size: 94 341 493 653
Fragments arranged by size:
653 493 341 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 530 1581
Size: 530 1051
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 371 576 1276 1298 1581
Size: 107 264 205 700 22 283
Fragments arranged by size:
700 283 264 205 107 22
Bpu10I CC'TnA_GC
Cuts at: 0 980 1581
Size: 980 601
BsaBI GATnn'nnATC
Cuts at: 0 623 645 1581
Size: 623 22 936
Fragments arranged by size:
936 623 22
BsaWI w'CCGG_w
Cuts at: 0 534 956 1233 1543 1581
Size: 534 422 277 310 38
Fragments arranged by size:
534 422 310 277 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 549 579 1581
Size: 549 30 1002
Fragments arranged by size:
1002 549 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1558 1581
Size: 1558 23
BsiHKAI G_wGCw'C
Cuts at: 0 1143 1203 1233 1262 1581
Size: 1143 60 30 29 319
Fragments arranged by size:
1143 319 60 30 29
BsmI GAATG_Cn'
Cuts at: 0 1333 1524 1581
Size: 1333 191 57
Fragments arranged by size:
1333 191 57
Bsp1286I G_dGCh'C
Cuts at: 0 137 146 1143 1203 1233 1262 1581
Size: 137 9 997 60 30 29 319
Fragments arranged by size:
997 319 137 60 30 29 9
BspEI T'CCGG_A
Cuts at: 0 534 1233 1543 1581
Size: 534 699 310 38
Fragments arranged by size:
699 534 310 38
BspHI T'CATG_A
Cuts at: 0 848 1581
Size: 848 733
BsrDI GCAATG_nn'
Cuts at: 0 803 1385 1581
Size: 803 582 196
Fragments arranged by size:
803 582 196
BsrFI r'CCGG_y
Cuts at: 0 1390 1581
Size: 1390 191
BssSI C'ACGA_G
Cuts at: 0 386 1581
Size: 386 1195
BstEII G'GTnAC_C
Cuts at: 0 94 1581
Size: 94 1487
BstXI CCAn_nnnn'nTGG
Cuts at: 0 637 1276 1581
Size: 637 639 305
Fragments arranged by size:
639 637 305
BstYI r'GATC_y
Cuts at: 0 15 429 468 531 768 789 1546 1581
Size: 15 414 39 63 237 21 757 35
Fragments arranged by size:
757 414 237 63 39 35 21 15
BtgI C'CryG_G
Cuts at: 0 138 1581
Size: 138 1443
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 309 610 1075 1581
Size: 309 301 465 506
Fragments arranged by size:
506 465 309 301
BtsI GCAGTG_nn'
Cuts at: 0 404 1298 1581
Size: 404 894 283
Fragments arranged by size:
894 404 283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1176 1211 1581
Size: 1176 35 370
Fragments arranged by size:
1176 370 35
EaeI y'GGCC_r
Cuts at: 0 1148 1392 1581
Size: 1148 244 189
Fragments arranged by size:
1148 244 189
EarI CTCTTCn'nnn_
Cuts at: 0 1490 1581
Size: 1490 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 371 547 576 1036 1276 1298 1420
Size: 107 264 176 29 460 240 22 122
Cuts at: 1420 1581
Size: 161
Fragments arranged by size:
460 264 240 176 161 122 107 29
22
EcoO109I rG'GnC_Cy
Cuts at: 0 133 134 143 239 381 1099 1581
Size: 133 1 9 96 142 718 482
Fragments arranged by size:
718 482 142 133 96 9 1
EcoRI G'AATT_C
Cuts at: 0 203 1581
Size: 203 1378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 549 581 843 875 1356 1388 1581
Size: 549 32 262 32 481 32 193
Fragments arranged by size:
549 481 262 193 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 1581
Size: 417 1164
MscI TGG'CCA
Cuts at: 0 1150 1581
Size: 1150 431
MslI CAynn'nnrTG
Cuts at: 0 635 1534 1581
Size: 635 899 47
Fragments arranged by size:
899 635 47
MspA1I CmG'CkG
Cuts at: 0 54 367 1581
Size: 54 313 1214
Fragments arranged by size:
1214 313 54
NaeI GCC'GGC
Cuts at: 0 1392 1581
Size: 1392 189
NgoMIV G'CCGG_C
Cuts at: 0 1390 1581
Size: 1390 191
NspI r_CATG'y
Cuts at: 0 226 1017 1182 1581
Size: 226 791 165 399
Fragments arranged by size:
791 399 226 165
PflMI CCAn_nnn'nTGG
Cuts at: 0 905 1095 1581
Size: 905 190 486
Fragments arranged by size:
905 486 190
PfoI T'CCnGG_A
Cuts at: 0 785 826 1581
Size: 785 41 755
Fragments arranged by size:
785 755 41
PpuMI rG'GwC_Cy
Cuts at: 0 381 1099 1581
Size: 381 718 482
Fragments arranged by size:
718 482 381
PsiI TTA'TAA
Cuts at: 0 707 1581
Size: 707 874
PspOMI G'GGCC_C
Cuts at: 0 133 142 1581
Size: 133 9 1439
Fragments arranged by size:
1439 133 9
PspXI vC'TCGA_Gb
Cuts at: 0 1142 1581
Size: 1142 439
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1117 1149 1581
Size: 1117 32 432
Fragments arranged by size:
1117 432 32
PvuII CAG'CTG
Cuts at: 0 367 1581
Size: 367 1214
SanDI GG'GwC_CC
Cuts at: 0 381 1099 1581
Size: 381 718 482
Fragments arranged by size:
718 482 381
SapI GCTCTTCn'nnn_
Cuts at: 0 1490 1581
Size: 1490 91
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 141 1581
Size: 141 1440
SmlI C'TyrA_G
Cuts at: 0 1142 1581
Size: 1142 439
SphI G_CATG'C
Cuts at: 0 226 1182 1581
Size: 226 956 399
Fragments arranged by size:
956 399 226
SspI AAT'ATT
Cuts at: 0 1273 1581
Size: 1273 308
StyI C'CwwG_G
Cuts at: 0 284 899 1581
Size: 284 615 682
Fragments arranged by size:
682 615 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 209 1581
Size: 209 1372
TatI w'GTAC_w
Cuts at: 0 1496 1581
Size: 1496 85
Tth111I GACn'n_nGTC
Cuts at: 0 541 1581
Size: 541 1040
XhoI C'TCGA_G
Cuts at: 0 1142 1581
Size: 1142 439
XmnI GAAnn'nnTTC
Cuts at: 0 872 1581
Size: 872 709
Enzymes that do cut:
AclI AcuI AleI AlfI ApaI ApoI AvaI BamHI
BanII BbsI BbvCI BcgI BglI Bme1580I BmrI BplI
BpmI Bpu10I BsaBI BsaWI BsaXI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspHI BsrDI BsrFI BssSI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI EaeI EarI Eco57MI
EcoO109I EcoRI Hin4I MmeI MscI MslI MspA1I NaeI
NgoMIV NspI PflMI PfoI PpuMI PsiI PspOMI PspXI
PsrI PvuII SanDI SapI SfiI SmlI SphI SspI
StyI TaqII TatI Tth111I XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BpuEI BsaI BsaAI BsaHI BseRI BseYI
BsiEI BsiWI BsmBI BspMI BsrBI BsrGI BssHII BstAPI
BstBI BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PstI PvuI
RsrII SacI SacII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI TstI XbaI XcmI XmaI ZraI