Restriction map for
pF1KE1963
(Linear) (Six-base) MAPSORT of: KIEE1963.seq Check: 9427 from: 1 to: 1572
KIEE1963.orf
With 182 enzymes: SgfI *
October 5, 2016 15:26 ..
AatII G_ACGT'C
Cuts at: 0 905 1572
Size: 905 667
AccI GT'mk_AC
Cuts at: 0 785 1370 1572
Size: 785 585 202
Fragments arranged by size:
785 585 202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 846 937 1374 1572
Size: 817 29 91 437 198
Fragments arranged by size:
817 437 198 91 29
AfeI AGC'GCT
Cuts at: 0 859 1572
Size: 859 713
AflIII A'CryG_T
Cuts at: 0 1028 1572
Size: 1028 544
ApaI G_GGCC'C
Cuts at: 0 104 457 563 1572
Size: 104 353 106 1009
Fragments arranged by size:
1009 353 106 104
ApaLI G'TGCA_C
Cuts at: 0 1008 1572
Size: 1008 564
AvaI C'yCGr_G
Cuts at: 0 96 271 342 371 481 654 968 1572
Size: 96 175 71 29 110 173 314 604
Fragments arranged by size:
604 314 175 173 110 96 71 29
BanI G'GyrC_C
Cuts at: 0 91 1246 1572
Size: 91 1155 326
Fragments arranged by size:
1155 326 91
BanII G_rGCy'C
Cuts at: 0 104 272 457 563 1277 1572
Size: 104 168 185 106 714 295
Fragments arranged by size:
714 295 185 168 106 104
BbeI G_GCGC'C
Cuts at: 0 95 1572
Size: 95 1477
BbsI GAAGACnn'nnnn_
Cuts at: 0 884 1572
Size: 884 688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 245 279 748 782 1572
Size: 245 34 469 34 790
Fragments arranged by size:
790 469 245 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 73 113 988 1572
Size: 73 40 875 584
Fragments arranged by size:
875 584 73 40
Bme1580I G_kGCm'C
Cuts at: 0 104 457 563 1012 1085 1091 1572
Size: 104 353 106 449 73 6 481
Fragments arranged by size:
481 449 353 106 104 73 6
BmgBI CAC'GTC
Cuts at: 0 543 1482 1572
Size: 543 939 90
Fragments arranged by size:
939 543 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 1008 1572
Size: 1008 564
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 921 1572
Size: 921 651
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 484 856 874 916 1537 1572
Size: 304 180 372 18 42 621 35
Fragments arranged by size:
621 372 304 180 42 35 18
Bpu10I CC'TnA_GC
Cuts at: 0 1052 1411 1572
Size: 1052 359 161
Fragments arranged by size:
1052 359 161
BsaI GGTCTCn'nnnn_
Cuts at: 0 415 1572
Size: 415 1157
BsaAI yAC'GTr
Cuts at: 0 1086 1572
Size: 1086 486
BsaHI Gr'CG_yC
Cuts at: 0 14 65 92 212 902 1141 1397 1572
Size: 14 51 27 120 690 239 256 175
Fragments arranged by size:
690 256 239 175 120 51 27 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 102 132 1572
Size: 102 30 1440
Fragments arranged by size:
1440 102 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 316 775 1309 1572
Size: 316 459 534 263
Fragments arranged by size:
534 459 316 263
BseYI C'CCAG_C
Cuts at: 0 103 141 448 890 1077 1572
Size: 103 38 307 442 187 495
Fragments arranged by size:
495 442 307 187 103 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1264 1357 1572
Size: 1264 93 215
Fragments arranged by size:
1264 215 93
BsiEI CG_ry'CG
Cuts at: 0 251 1104 1334 1572
Size: 251 853 230 238
Fragments arranged by size:
853 251 238 230
BsiHKAI G_wGCw'C
Cuts at: 0 833 1012 1277 1572
Size: 833 179 265 295
Fragments arranged by size:
833 295 265 179
BsmBI CGTCTCn'nnnn_
Cuts at: 0 385 909 1572
Size: 385 524 663
Fragments arranged by size:
663 524 385
Bsp1286I G_dGCh'C
Cuts at: 0 104 272 457 563 833 1012 1085 1091
Size: 104 168 185 106 270 179 73 6
Cuts at: 1091 1277 1572
Size: 186 295
Fragments arranged by size:
295 270 186 185 179 168 106 104
73 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 338 1572
Size: 338 1234
BsrBI CCG'CTC
Cuts at: 0 225 1572
Size: 225 1347
BsrFI r'CCGG_y
Cuts at: 0 952 1572
Size: 952 620
BssSI C'ACGA_G
Cuts at: 0 1069 1572
Size: 1069 503
BstEII G'GTnAC_C
Cuts at: 0 618 1572
Size: 618 954
Bsu36I CC'TnA_GG
Cuts at: 0 1322 1572
Size: 1322 250
BtgI C'CryG_G
Cuts at: 0 121 175 554 1572
Size: 121 54 379 1018
Fragments arranged by size:
1018 379 121 54
BtsI GCAGTG_nn'
Cuts at: 0 1281 1572
Size: 1281 291
DrdI GACnn_nn'nnGTC
Cuts at: 0 1362 1572
Size: 1362 210
EaeI y'GGCC_r
Cuts at: 0 248 503 950 980 1097 1331 1472 1572
Size: 248 255 447 30 117 234 141 100
Fragments arranged by size:
447 255 248 234 141 117 100 30
EagI C'GGCC_G
Cuts at: 0 248 1331 1572
Size: 248 1083 241
Fragments arranged by size:
1083 248 241
EarI CTCTTCn'nnn_
Cuts at: 0 340 1572
Size: 340 1232
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 954 1012 1572
Size: 954 58 560
Fragments arranged by size:
954 560 58
EcoICRI GAG'CTC
Cuts at: 0 1275 1572
Size: 1275 297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 484 817 846 856 874 916 937
Size: 304 180 333 29 10 18 42 21
Cuts at: 937 1374 1537 1572
Size: 437 163 35
Fragments arranged by size:
437 333 304 180 163 42 35 29
21 18 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 205 1291 1572
Size: 205 1086 281
Fragments arranged by size:
1086 281 205
EcoO109I rG'GnC_Cy
Cuts at: 0 100 170 276 453 454 498 559 567
Size: 100 70 106 177 1 44 61 8
Cuts at: 567 801 1151 1572
Size: 234 350 421
Fragments arranged by size:
421 350 234 177 106 100 70 61
44 8 1
FseI GG_CCGG'CC
Cuts at: 0 956 1572
Size: 956 616
FspI TGC'GCA
Cuts at: 0 547 1572
Size: 547 1025
HaeII r_GCGC'y
Cuts at: 0 95 367 861 1440 1512 1572
Size: 95 272 494 579 72 60
Fragments arranged by size:
579 494 272 95 72 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1162 1194 1572
Size: 1162 32 378
Fragments arranged by size:
1162 378 32
KasI G'GCGC_C
Cuts at: 0 91 1572
Size: 91 1481
MluI A'CGCG_T
Cuts at: 0 1028 1572
Size: 1028 544
MscI TGG'CCA
Cuts at: 0 982 1474 1572
Size: 982 492 98
Fragments arranged by size:
982 492 98
MslI CAynn'nnrTG
Cuts at: 0 1480 1572
Size: 1480 92
MspA1I CmG'CkG
Cuts at: 0 145 177 247 253 556 866 894 939
Size: 145 32 70 6 303 310 28 45
Cuts at: 939 1572
Size: 633
Fragments arranged by size:
633 310 303 145 70 45 32 28
6
NaeI GCC'GGC
Cuts at: 0 954 1572
Size: 954 618
NarI GG'CG_CC
Cuts at: 0 92 1572
Size: 92 1480
NgoMIV G'CCGG_C
Cuts at: 0 952 1572
Size: 952 620
NotI GC'GGCC_GC
Cuts at: 0 248 1572
Size: 248 1324
PasI CC'CwG_GG
Cuts at: 0 1146 1572
Size: 1146 426
PfoI T'CCnGG_A
Cuts at: 0 1526 1572
Size: 1526 46
PmlI CAC'GTG
Cuts at: 0 1086 1572
Size: 1086 486
PpuMI rG'GwC_Cy
Cuts at: 0 170 276 498 567 1572
Size: 170 106 222 69 1005
Fragments arranged by size:
1005 222 170 106 69
PshAI GACnn'nnGTC
Cuts at: 0 605 1572
Size: 605 967
PspOMI G'GGCC_C
Cuts at: 0 100 453 559 1572
Size: 100 353 106 1013
Fragments arranged by size:
1013 353 106 100
PspXI vC'TCGA_Gb
Cuts at: 0 481 1572
Size: 481 1091
PstI C_TGCA'G
Cuts at: 0 138 258 1572
Size: 138 120 1314
Fragments arranged by size:
1314 138 120
PvuII CAG'CTG
Cuts at: 0 145 894 939 1572
Size: 145 749 45 633
Fragments arranged by size:
749 633 145 45
SacI G_AGCT'C
Cuts at: 0 1277 1572
Size: 1277 295
SacII CC_GC'GG
Cuts at: 0 178 557 1572
Size: 178 379 1015
Fragments arranged by size:
1015 379 178
SanDI GG'GwC_CC
Cuts at: 0 276 1572
Size: 276 1296
SapI GCTCTTCn'nnn_
Cuts at: 0 340 1572
Size: 340 1232
ScaI AGT'ACT
Cuts at: 0 469 1572
Size: 469 1103
SexAI A'CCwGG_T
Cuts at: 0 614 668 1572
Size: 614 54 904
Fragments arranged by size:
904 614 54
SfcI C'TryA_G
Cuts at: 0 134 254 1572
Size: 134 120 1318
Fragments arranged by size:
1318 134 120
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 988 1572
Size: 988 584
SfoI GGC'GCC
Cuts at: 0 93 1572
Size: 93 1479
SmaI CCC'GGG
Cuts at: 0 98 273 656 970 1572
Size: 98 175 383 314 602
Fragments arranged by size:
602 383 314 175 98
SmlI C'TyrA_G
Cuts at: 0 481 1572
Size: 481 1091
StuI AGG'CCT
Cuts at: 0 841 1240 1572
Size: 841 399 332
Fragments arranged by size:
841 399 332
StyI C'CwwG_G
Cuts at: 0 32 166 563 1400 1572
Size: 32 134 397 837 172
Fragments arranged by size:
837 397 172 134 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1027 1572
Size: 1027 545
TatI w'GTAC_w
Cuts at: 0 467 716 749 1187 1572
Size: 467 249 33 438 385
Fragments arranged by size:
467 438 385 249 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 102 134 1572
Size: 102 32 1438
Fragments arranged by size:
1438 102 32
XbaI T'CTAG_A
Cuts at: 0 417 1572
Size: 417 1155
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1482 1572
Size: 1482 90
XhoI C'TCGA_G
Cuts at: 0 481 1572
Size: 481 1091
XmaI C'CCGG_G
Cuts at: 0 96 271 654 968 1572
Size: 96 175 383 314 604
Fragments arranged by size:
604 383 314 175 96
ZraI GAC'GTC
Cuts at: 0 903 1572
Size: 903 669
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII ApaI ApaLI AvaI
BanI BanII BbeI BbsI BcgI BglI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssSI BstEII Bsu36I BtgI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FseI FspI HaeII Hin4I KasI MluI MscI MslI
MspA1I NaeI NarI NgoMIV NotI PasI PfoI PmlI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII
SanDI SapI ScaI SexAI SfcI SfiI SfoI SmaI
SmlI StuI StyI TaqII TatI TstI XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmtI
BpuEI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BssHII BstAPI BstBI BstXI BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII EcoRI EcoRV FalI FspAI
HincII HindIII HpaI KpnI MfeI MmeI NcoI NdeI
NheI NruI NsiI NspI PacI PciI PflMI PmeI
PpiI PsiI PsrI PvuI RsrII SalI SbfI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XmnI