Restriction map for
pF1KE1965
(Linear) (Six-base) MAPSORT of: KIEE1965.seq Check: 3360 from: 1 to: 1587
KIEE1965.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1544 1587
Size: 926 618 43
Fragments arranged by size:
926 618 43
AhdI GACnn_n'nnGTC
Cuts at: 0 231 1587
Size: 231 1356
AleI CACnn'nnGTG
Cuts at: 0 217 1587
Size: 217 1370
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 647 679 1587
Size: 647 32 908
Fragments arranged by size:
908 647 32
AlwNI CAG_nnn'CTG
Cuts at: 0 672 1587
Size: 672 915
ApoI r'AATT_y
Cuts at: 0 397 955 1587
Size: 397 558 632
Fragments arranged by size:
632 558 397
AvaI C'yCGr_G
Cuts at: 0 877 1587
Size: 877 710
BanI G'GyrC_C
Cuts at: 0 1208 1587
Size: 1208 379
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 361 395 1587
Size: 361 34 1192
Fragments arranged by size:
1192 361 34
BfrBI ATG'CAT
Cuts at: 0 537 1171 1587
Size: 537 634 416
Fragments arranged by size:
634 537 416
BglI GCCn_nnn'nGGC
Cuts at: 0 1474 1587
Size: 1474 113
BglII A'GATC_T
Cuts at: 0 1329 1587
Size: 1329 258
Bme1580I G_kGCm'C
Cuts at: 0 384 1587
Size: 384 1203
BmrI ACTGGGnnnn_n'
Cuts at: 0 1064 1587
Size: 1064 523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 603 1587
Size: 603 984
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 120 150 1587
Size: 120 30 1437
Fragments arranged by size:
1437 120 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 66 798 853 1531 1587
Size: 66 732 55 678 56
Fragments arranged by size:
732 678 66 56 55
BseYI C'CCAG_C
Cuts at: 0 792 1587
Size: 792 795
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 1587
Size: 318 1269
BsiHKAI G_wGCw'C
Cuts at: 0 274 1587
Size: 274 1313
BsmI GAATG_Cn'
Cuts at: 0 106 1587
Size: 106 1481
BsmBI CGTCTCn'nnnn_
Cuts at: 0 336 1587
Size: 336 1251
Bsp1286I G_dGCh'C
Cuts at: 0 274 384 1587
Size: 274 110 1203
Fragments arranged by size:
1203 274 110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 271 1587
Size: 271 1316
BsrBI CCG'CTC
Cuts at: 0 119 1587
Size: 119 1468
BsrDI GCAATG_nn'
Cuts at: 0 873 1587
Size: 873 714
BsrGI T'GTAC_A
Cuts at: 0 1295 1587
Size: 1295 292
BstXI CCAn_nnnn'nTGG
Cuts at: 0 274 1332 1587
Size: 274 1058 255
Fragments arranged by size:
1058 274 255
BstYI r'GATC_y
Cuts at: 0 571 1135 1329 1418 1587
Size: 571 564 194 89 169
Fragments arranged by size:
571 564 194 169 89
BtgI C'CryG_G
Cuts at: 0 112 992 1587
Size: 112 880 595
Fragments arranged by size:
880 595 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1459 1587
Size: 1459 128
BtsI GCAGTG_nn'
Cuts at: 0 559 1201 1463 1587
Size: 559 642 262 124
Fragments arranged by size:
642 559 262 124
DrdI GACnn_nn'nnGTC
Cuts at: 0 526 1587
Size: 526 1061
EaeI y'GGCC_r
Cuts at: 0 51 138 468 1587
Size: 51 87 330 1119
Fragments arranged by size:
1119 330 87 51
EarI CTCTTCn'nnn_
Cuts at: 0 643 945 1223 1587
Size: 643 302 278 364
Fragments arranged by size:
643 364 302 278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 603 926 1544 1587
Size: 603 323 618 43
Fragments arranged by size:
618 603 323 43
EcoO109I rG'GnC_Cy
Cuts at: 0 260 461 883 1587
Size: 260 201 422 704
Fragments arranged by size:
704 422 260 201
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 333 1587
Size: 333 1254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 56 88 217 249 563 595 1357 1389
Size: 56 32 129 32 314 32 762 32
Cuts at: 1389 1587
Size: 198
Fragments arranged by size:
762 314 198 129 56 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1179 1587
Size: 1179 408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1396 1587
Size: 1396 191
MscI TGG'CCA
Cuts at: 0 53 140 470 1587
Size: 53 87 330 1117
Fragments arranged by size:
1117 330 87 53
MslI CAynn'nnrTG
Cuts at: 0 217 542 1105 1587
Size: 217 325 563 482
Fragments arranged by size:
563 482 325 217
MspA1I CmG'CkG
Cuts at: 0 49 946 1587
Size: 49 897 641
Fragments arranged by size:
897 641 49
NcoI C'CATG_G
Cuts at: 0 992 1587
Size: 992 595
NsiI A_TGCA'T
Cuts at: 0 539 1173 1587
Size: 539 634 414
Fragments arranged by size:
634 539 414
NspI r_CATG'y
Cuts at: 0 1086 1587
Size: 1086 501
PflMI CCAn_nnn'nTGG
Cuts at: 0 1054 1587
Size: 1054 533
PfoI T'CCnGG_A
Cuts at: 0 900 1587
Size: 900 687
PpuMI rG'GwC_Cy
Cuts at: 0 260 461 883 1587
Size: 260 201 422 704
Fragments arranged by size:
704 422 260 201
PshAI GACnn'nnGTC
Cuts at: 0 128 1587
Size: 128 1459
PsiI TTA'TAA
Cuts at: 0 254 1587
Size: 254 1333
PvuII CAG'CTG
Cuts at: 0 946 1587
Size: 946 641
SfcI C'TryA_G
Cuts at: 0 825 1344 1587
Size: 825 519 243
Fragments arranged by size:
825 519 243
SphI G_CATG'C
Cuts at: 0 1086 1587
Size: 1086 501
SspI AAT'ATT
Cuts at: 0 513 1587
Size: 513 1074
StyI C'CwwG_G
Cuts at: 0 625 928 992 1434 1587
Size: 625 303 64 442 153
Fragments arranged by size:
625 442 303 153 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 507 1263 1587
Size: 507 756 324
Fragments arranged by size:
756 507 324
TatI w'GTAC_w
Cuts at: 0 17 749 842 1295 1587
Size: 17 732 93 453 292
Fragments arranged by size:
732 453 292 93 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 120 152 1587
Size: 120 32 1435
Fragments arranged by size:
1435 120 32
Tth111I GACn'n_nGTC
Cuts at: 0 445 1587
Size: 445 1142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 46 478 707 982 1587
Size: 46 432 229 275 605
Fragments arranged by size:
605 432 275 229 46
Enzymes that do cut:
AcuI AhdI AleI AloI AlwNI ApoI AvaI BanI
BcgI BfrBI BglI BglII Bme1580I BmrI BpmI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrGI BstXI BstYI BtgI BtgZI BtsI
DrdI EaeI EarI Eco57MI EcoO109I FalI Hin4I HincII
MmeI MscI MslI MspA1I NcoI NsiI NspI PflMI
PfoI PpuMI PshAI PsiI PvuII SfcI SphI SspI
StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbeI BbsI BbvCI BciVI BclI
BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BspHI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PmeI PmlI PpiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI
SwaI XbaI XhoI XmaI XmnI ZraI