Restriction map for
pF1KE1989
(Linear) (Six-base) MAPSORT of: KIEE1989.seq Check: 3037 from: 1 to: 1695
KIEE1989.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 0 803 1559 1695
Size: 803 756 136
Fragments arranged by size:
803 756 136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 993 1695
Size: 993 702
AfeI AGC'GCT
Cuts at: 0 814 925 1474 1695
Size: 814 111 549 221
Fragments arranged by size:
814 549 221 111
AflIII A'CryG_T
Cuts at: 0 721 1695
Size: 721 974
AleI CACnn'nnGTG
Cuts at: 0 294 865 952 1695
Size: 294 571 87 743
Fragments arranged by size:
743 571 294 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 312 539 970 1648 1695
Size: 34 278 227 431 678 47
Fragments arranged by size:
678 431 278 227 47 34
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 302 334 1695
Size: 302 32 1361
Fragments arranged by size:
1361 302 32
ApaLI G'TGCA_C
Cuts at: 0 138 217 297 1695
Size: 138 79 80 1398
Fragments arranged by size:
1398 138 80 79
AvaI C'yCGr_G
Cuts at: 0 397 537 546 1695
Size: 397 140 9 1149
Fragments arranged by size:
1149 397 140 9
BamHI G'GATC_C
Cuts at: 0 740 1695
Size: 740 955
BanI G'GyrC_C
Cuts at: 0 433 523 532 618 961 1345 1695
Size: 433 90 9 86 343 384 350
Fragments arranged by size:
433 384 350 343 90 86 9
BanII G_rGCy'C
Cuts at: 0 480 1695
Size: 480 1215
BbeI G_GCGC'C
Cuts at: 0 437 1695
Size: 437 1258
BbsI GAAGACnn'nnnn_
Cuts at: 0 1383 1695
Size: 1383 312
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 129 163 1695
Size: 129 34 1532
Fragments arranged by size:
1532 129 34
BclI T'GATC_A
Cuts at: 0 1565 1695
Size: 1565 130
BglI GCCn_nnn'nGGC
Cuts at: 0 64 551 1096 1129 1138 1695
Size: 64 487 545 33 9 557
Fragments arranged by size:
557 545 487 64 33 9
BglII A'GATC_T
Cuts at: 0 443 1695
Size: 443 1252
BlpI GC'TnA_GC
Cuts at: 0 1192 1695
Size: 1192 503
Bme1580I G_kGCm'C
Cuts at: 0 142 221 301 535 964 1415 1519 1695
Size: 142 79 80 234 429 451 104 176
Fragments arranged by size:
451 429 234 176 142 104 80 79
BmgBI CAC'GTC
Cuts at: 0 1283 1601 1695
Size: 1283 318 94
Fragments arranged by size:
1283 318 94
BmrI ACTGGGnnnn_n'
Cuts at: 0 63 1476 1695
Size: 63 1413 219
Fragments arranged by size:
1413 219 63
BmtI G_CTAG'C
Cuts at: 0 1252 1695
Size: 1252 443
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 945 1119 1695
Size: 945 174 576
Fragments arranged by size:
945 576 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 883 1384 1695
Size: 604 279 501 311
Fragments arranged by size:
604 501 311 279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 1695
Size: 598 1097
BsaI GGTCTCn'nnnn_
Cuts at: 0 1678 1695
Size: 1678 17
BsaAI yAC'GTr
Cuts at: 0 794 1695
Size: 794 901
BsaHI Gr'CG_yC
Cuts at: 0 434 1388 1695
Size: 434 954 307
Fragments arranged by size:
954 434 307
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1462 1492 1695
Size: 1462 30 203
Fragments arranged by size:
1462 203 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 730 1695
Size: 730 965
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 963 976 1618 1695
Size: 265 698 13 642 77
Fragments arranged by size:
698 642 265 77 13
BsiEI CG_ry'CG
Cuts at: 0 1142 1695
Size: 1142 553
BsiHKAI G_wGCw'C
Cuts at: 0 142 221 253 280 301 419 480 590
Size: 142 79 32 27 21 118 61 110
Cuts at: 590 899 1403 1466 1695
Size: 309 504 63 229
Fragments arranged by size:
504 309 229 142 118 110 79 63
61 32 27 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 712 1695
Size: 712 983
Bsp1286I G_dGCh'C
Cuts at: 0 142 221 253 280 301 419 480 535
Size: 142 79 32 27 21 118 61 55
Cuts at: 535 590 899 964 1403 1415 1466 1519 1695
Size: 55 309 65 439 12 51 53 176
Fragments arranged by size:
439 309 176 142 118 79 65 61
55 55 53 51 32 27 21 12
BspHI T'CATG_A
Cuts at: 0 371 992 1331 1577 1695
Size: 371 621 339 246 118
Fragments arranged by size:
621 371 339 246 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 687 1196 1695
Size: 687 509 499
Fragments arranged by size:
687 509 499
BsrBI CCG'CTC
Cuts at: 0 332 831 1196 1257 1368 1695
Size: 332 499 365 61 111 327
Fragments arranged by size:
499 365 332 327 111 61
BsrFI r'CCGG_y
Cuts at: 0 63 1066 1137 1297 1695
Size: 63 1003 71 160 398
Fragments arranged by size:
1003 398 160 71 63
BssHII G'CGCG_C
Cuts at: 0 345 347 349 730 1695
Size: 345 2 2 381 965
Fragments arranged by size:
965 381 345 2 2
BssSI C'ACGA_G
Cuts at: 0 1084 1476 1638 1695
Size: 1084 392 162 57
Fragments arranged by size:
1084 392 162 57
BstEII G'GTnAC_C
Cuts at: 0 940 1695
Size: 940 755
BstXI CCAn_nnnn'nTGG
Cuts at: 0 180 798 1695
Size: 180 618 897
Fragments arranged by size:
897 618 180
BstYI r'GATC_y
Cuts at: 0 443 740 1613 1695
Size: 443 297 873 82
Fragments arranged by size:
873 443 297 82
BtgI C'CryG_G
Cuts at: 0 75 105 429 863 1166 1695
Size: 75 30 324 434 303 529
Fragments arranged by size:
529 434 324 303 75 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 132 867 1413 1695
Size: 132 735 546 282
Fragments arranged by size:
735 546 282 132
BtsI GCAGTG_nn'
Cuts at: 0 253 597 1491 1695
Size: 253 344 894 204
Fragments arranged by size:
894 344 253 204
DrdI GACnn_nn'nnGTC
Cuts at: 0 1555 1695
Size: 1555 140
EaeI y'GGCC_r
Cuts at: 0 453 1016 1121 1139 1163 1295 1505 1695
Size: 453 563 105 18 24 132 210 190
Fragments arranged by size:
563 453 210 190 132 105 24 18
EagI C'GGCC_G
Cuts at: 0 1139 1695
Size: 1139 556
EarI CTCTTCn'nnn_
Cuts at: 0 994 1264 1318 1695
Size: 994 270 54 377
Fragments arranged by size:
994 377 270 54
EcoICRI GAG'CTC
Cuts at: 0 478 1695
Size: 478 1217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 883 993 1384 1695
Size: 604 279 110 391 311
Fragments arranged by size:
604 391 311 279 110
FseI GG_CCGG'CC
Cuts at: 0 67 1695
Size: 67 1628
FspI TGC'GCA
Cuts at: 0 1381 1695
Size: 1381 314
FspAI rTGC'GCAy
Cuts at: 0 1381 1695
Size: 1381 314
HaeII r_GCGC'y
Cuts at: 0 17 405 437 563 816 927 1255 1476
Size: 17 388 32 126 253 111 328 221
Cuts at: 1476 1695
Size: 219
Fragments arranged by size:
388 328 253 221 219 126 111 32
17
KasI G'GCGC_C
Cuts at: 0 433 1695
Size: 433 1262
MluI A'CGCG_T
Cuts at: 0 721 1695
Size: 721 974
MscI TGG'CCA
Cuts at: 0 1018 1165 1507 1695
Size: 1018 147 342 188
Fragments arranged by size:
1018 342 188 147
MslI CAynn'nnrTG
Cuts at: 0 294 865 952 1330 1695
Size: 294 571 87 378 365
Fragments arranged by size:
571 378 365 294 87
MspA1I CmG'CkG
Cuts at: 0 39 135 234 1233 1695
Size: 39 96 99 999 462
Fragments arranged by size:
999 462 99 96 39
NaeI GCC'GGC
Cuts at: 0 65 1068 1139 1299 1695
Size: 65 1003 71 160 396
Fragments arranged by size:
1003 396 160 71 65
NarI GG'CG_CC
Cuts at: 0 434 1695
Size: 434 1261
NcoI C'CATG_G
Cuts at: 0 863 1166 1695
Size: 863 303 529
Fragments arranged by size:
863 529 303
NgoMIV G'CCGG_C
Cuts at: 0 63 1066 1137 1297 1695
Size: 63 1003 71 160 398
Fragments arranged by size:
1003 398 160 71 63
NheI G'CTAG_C
Cuts at: 0 1248 1695
Size: 1248 447
NspI r_CATG'y
Cuts at: 0 810 1542 1695
Size: 810 732 153
Fragments arranged by size:
810 732 153
PflMI CCAn_nnn'nTGG
Cuts at: 0 1031 1695
Size: 1031 664
PfoI T'CCnGG_A
Cuts at: 0 98 899 1695
Size: 98 801 796
Fragments arranged by size:
801 796 98
PstI C_TGCA'G
Cuts at: 0 518 1695
Size: 518 1177
SacI G_AGCT'C
Cuts at: 0 480 1695
Size: 480 1215
SapI GCTCTTCn'nnn_
Cuts at: 0 1264 1318 1695
Size: 1264 54 377
Fragments arranged by size:
1264 377 54
ScaI AGT'ACT
Cuts at: 0 1111 1695
Size: 1111 584
SexAI A'CCwGG_T
Cuts at: 0 748 1051 1695
Size: 748 303 644
Fragments arranged by size:
748 644 303
SfcI C'TryA_G
Cuts at: 0 514 1695
Size: 514 1181
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1096 1129 1138 1695
Size: 1096 33 9 557
Fragments arranged by size:
1096 557 33 9
SfoI GGC'GCC
Cuts at: 0 435 1695
Size: 435 1260
SmaI CCC'GGG
Cuts at: 0 539 548 1695
Size: 539 9 1147
Fragments arranged by size:
1147 539 9
SmlI C'TyrA_G
Cuts at: 0 613 1695
Size: 613 1082
SrfI GCCC'GGGC
Cuts at: 0 548 1695
Size: 548 1147
StuI AGG'CCT
Cuts at: 0 209 1214 1453 1695
Size: 209 1005 239 242
Fragments arranged by size:
1005 242 239 209
StyI C'CwwG_G
Cuts at: 0 863 1166 1695
Size: 863 303 529
Fragments arranged by size:
863 529 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1369 1470 1695
Size: 1369 101 225
Fragments arranged by size:
1369 225 101
TatI w'GTAC_w
Cuts at: 0 1109 1695
Size: 1109 586
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1462 1494 1695
Size: 1462 32 201
Fragments arranged by size:
1462 201 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 870 1158 1164 1695
Size: 870 288 6 531
Fragments arranged by size:
870 531 288 6
XmaI C'CCGG_G
Cuts at: 0 537 546 1695
Size: 537 9 1149
Fragments arranged by size:
1149 537 9
XmnI GAAnn'nnTTC
Cuts at: 0 379 1695
Size: 379 1316
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AloI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BcgI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BssHII BssSI BstEII BstXI BstYI BtgI BtgZI BtsI
DrdI EaeI EagI EarI EcoICRI Eco57MI FseI FspI
FspAI HaeII KasI MluI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PflMI PfoI PstI
SacI SapI ScaI SexAI SfcI SfiI SfoI SmaI
SmlI SrfI StuI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AlwNI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BbvCI
BciVI BfrBI Bpu10I BsaBI BsaWI BseYI BsiWI BsmI
BspEI BsrDI BsrGI BstAPI BstBI BstZ17I Bsu36I ClaI
CspCI DraI DraIII EciI EcoNI EcoO109I EcoRI EcoRV
FalI Hin4I HincII HindIII HpaI KpnI MfeI MmeI
NdeI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SbfI SgfI
SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI
XhoI ZraI