Restriction map for
pF1KE2061
(Linear) (Six-base) MAPSORT of: KIEE2061.seq Check: 3861 from: 1 to: 2115
KIEE2061.orf
With 182 enzymes: SgfI *
January 30, 2017 10:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 282 2115
Size: 282 1833
AatII G_ACGT'C
Cuts at: 0 988 1893 2115
Size: 988 905 222
Fragments arranged by size:
988 905 222
AccI GT'mk_AC
Cuts at: 0 656 2115
Size: 656 1459
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 552 553 2115
Size: 267 285 1 1562
Fragments arranged by size:
1562 285 267 1
AfeI AGC'GCT
Cuts at: 0 1121 2115
Size: 1121 994
AflIII A'CryG_T
Cuts at: 0 1073 2115
Size: 1073 1042
AhdI GACnn_n'nnGTC
Cuts at: 0 1580 2115
Size: 1580 535
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1525 2115
Size: 1525 590
AlwNI CAG_nnn'CTG
Cuts at: 0 1251 1995 2115
Size: 1251 744 120
Fragments arranged by size:
1251 744 120
ApaI G_GGCC'C
Cuts at: 0 1323 1364 1678 1740 1768 1882 2115
Size: 1323 41 314 62 28 114 233
Fragments arranged by size:
1323 314 233 114 62 41 28
ApoI r'AATT_y
Cuts at: 0 439 727 2115
Size: 439 288 1388
Fragments arranged by size:
1388 439 288
AvaI C'yCGr_G
Cuts at: 0 136 202 210 1618 1875 2115
Size: 136 66 8 1408 257 240
Fragments arranged by size:
1408 257 240 136 66 8
BanI G'GyrC_C
Cuts at: 0 14 106 1623 2115
Size: 14 92 1517 492
Fragments arranged by size:
1517 492 92 14
BanII G_rGCy'C
Cuts at: 0 917 1226 1294 1323 1364 1678 1740 1768
Size: 917 309 68 29 41 314 62 28
Cuts at: 1768 1882 2115
Size: 114 233
Fragments arranged by size:
917 314 309 233 114 68 62 41
29 28
BbeI G_GCGC'C
Cuts at: 0 18 1627 2115
Size: 18 1609 488
Fragments arranged by size:
1609 488 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 1012 1208 2115
Size: 1012 196 907
Fragments arranged by size:
1012 907 196
BbvCI CC'TCA_GC
Cuts at: 0 1536 2115
Size: 1536 579
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1843 1877 2115
Size: 1843 34 238
Fragments arranged by size:
1843 238 34
BclI T'GATC_A
Cuts at: 0 741 2115
Size: 741 1374
BglI GCCn_nnn'nGGC
Cuts at: 0 178 187 1359 1673 1735 1793 1816 2115
Size: 178 9 1172 314 62 58 23 299
Fragments arranged by size:
1172 314 299 178 62 58 23 9
BlpI GC'TnA_GC
Cuts at: 0 775 1370 2115
Size: 775 595 745
Fragments arranged by size:
775 745 595
Bme1580I G_kGCm'C
Cuts at: 0 111 815 1323 1364 1678 1740 1768 1832
Size: 111 704 508 41 314 62 28 64
Cuts at: 1832 1882 2115
Size: 50 233
Fragments arranged by size:
704 508 314 233 111 64 62 50
41 28
BmgBI CAC'GTC
Cuts at: 0 939 1929 2115
Size: 939 990 186
Fragments arranged by size:
990 939 186
BmrI ACTGGGnnnn_n'
Cuts at: 0 384 626 724 1847 1983 2115
Size: 384 242 98 1123 136 132
Fragments arranged by size:
1123 384 242 136 132 98
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 177 2115
Size: 177 1938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 1051 1613 2115
Size: 321 730 562 502
Fragments arranged by size:
730 562 502 321
Bpu10I CC'TnA_GC
Cuts at: 0 1244 1536 2044 2115
Size: 1244 292 508 71
Fragments arranged by size:
1244 508 292 71
BsaI GGTCTCn'nnnn_
Cuts at: 0 533 2062 2115
Size: 533 1529 53
Fragments arranged by size:
1529 533 53
BsaAI yAC'GTr
Cuts at: 0 1076 2115
Size: 1076 1039
BsaHI Gr'CG_yC
Cuts at: 0 15 985 1624 1890 2115
Size: 15 970 639 266 225
Fragments arranged by size:
970 639 266 225 15
BseYI C'CCAG_C
Cuts at: 0 174 1346 1421 1494 1514 1544 1661 1788
Size: 174 1172 75 73 20 30 117 127
Cuts at: 1788 1867 1918 1973 2038 2115
Size: 79 51 55 65 77
Fragments arranged by size:
1172 174 127 117 79 77 75 73
65 55 51 30 20
BsiEI CG_ry'CG
Cuts at: 0 191 2115
Size: 191 1924
BsiHKAI G_wGCw'C
Cuts at: 0 1081 1226 2115
Size: 1081 145 889
Fragments arranged by size:
1081 889 145
BsmI GAATG_Cn'
Cuts at: 0 648 2115
Size: 648 1467
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1661 1710 2115
Size: 1661 49 405
Fragments arranged by size:
1661 405 49
Bsp1286I G_dGCh'C
Cuts at: 0 111 815 917 1081 1226 1294 1323 1364
Size: 111 704 102 164 145 68 29 41
Cuts at: 1364 1678 1740 1768 1832 1882 2115
Size: 314 62 28 64 50 233
Fragments arranged by size:
704 314 233 164 145 111 102 68
64 62 50 41 29 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 17 80 282 2115
Size: 17 63 202 1833
Fragments arranged by size:
1833 202 63 17
BsrBI CCG'CTC
Cuts at: 0 1179 2115
Size: 1179 936
BsrDI GCAATG_nn'
Cuts at: 0 1438 2115
Size: 1438 677
BsrFI r'CCGG_y
Cuts at: 0 1792 2115
Size: 1792 323
BssSI C'ACGA_G
Cuts at: 0 1225 2115
Size: 1225 890
BstEII G'GTnAC_C
Cuts at: 0 1186 2115
Size: 1186 929
BstXI CCAn_nnnn'nTGG
Cuts at: 0 472 955 2115
Size: 472 483 1160
Fragments arranged by size:
1160 483 472
BstYI r'GATC_y
Cuts at: 0 401 2115
Size: 401 1714
Bsu36I CC'TnA_GG
Cuts at: 0 2083 2115
Size: 2083 32
BtgI C'CryG_G
Cuts at: 0 260 321 405 794 1296 2115
Size: 260 61 84 389 502 819
Fragments arranged by size:
819 502 389 260 84 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 855 1039 2115
Size: 855 184 1076
Fragments arranged by size:
1076 855 184
BtsI GCAGTG_nn'
Cuts at: 0 146 905 1118 2115
Size: 146 759 213 997
Fragments arranged by size:
997 759 213 146
ClaI AT'CG_AT
Cuts at: 0 923 2115
Size: 923 1192
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 863 898 2115
Size: 863 35 1217
Fragments arranged by size:
1217 863 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 943 990 2115
Size: 943 47 1125
Fragments arranged by size:
1125 943 47
EaeI y'GGCC_r
Cuts at: 0 41 188 1299 1600 2115
Size: 41 147 1111 301 515
Fragments arranged by size:
1111 515 301 147 41
EagI C'GGCC_G
Cuts at: 0 188 2115
Size: 188 1927
EarI CTCTTCn'nnn_
Cuts at: 0 2110 2115
Size: 2110 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 544 1709 2115
Size: 544 1165 406
Fragments arranged by size:
1165 544 406
EcoICRI GAG'CTC
Cuts at: 0 1224 2115
Size: 1224 891
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 321 552 553 1051 1613 2115
Size: 267 54 231 1 498 562 502
Fragments arranged by size:
562 502 498 267 231 54 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 978 2047 2115
Size: 978 1069 68
Fragments arranged by size:
1069 978 68
EcoO109I rG'GnC_Cy
Cuts at: 0 158 206 1248 1319 1320 1360 1361 1417
Size: 158 48 1042 71 1 40 1 56
Cuts at: 1417 1432 1615 1675 1736 1737 1764 1822 1879
Size: 15 183 60 61 1 27 58 57
Cuts at: 1879 1960 2115
Size: 81 155
Fragments arranged by size:
1042 183 158 155 81 71 61 60
58 57 56 48 40 27 15 1
1 1
EcoRI G'AATT_C
Cuts at: 0 439 727 2115
Size: 439 288 1388
Fragments arranged by size:
1388 439 288
FspI TGC'GCA
Cuts at: 0 1110 2115
Size: 1110 1005
HaeII r_GCGC'y
Cuts at: 0 18 1123 1428 1452 1521 1543 1627 1668
Size: 18 1105 305 24 69 22 84 41
Cuts at: 1668 1761 2115
Size: 93 354
Fragments arranged by size:
1105 354 305 93 84 69 41 24
22 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1063 1088 1095 1120 1566 1598 2115
Size: 1063 25 7 25 446 32 517
Fragments arranged by size:
1063 517 446 32 25 25 7
HincII GTy'rAC
Cuts at: 0 639 846 1233 2115
Size: 639 207 387 882
Fragments arranged by size:
882 639 387 207
HpaI GTT'AAC
Cuts at: 0 639 2115
Size: 639 1476
KasI G'GCGC_C
Cuts at: 0 14 1623 2115
Size: 14 1609 492
Fragments arranged by size:
1609 492 14
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 266 2115
Size: 5 261 1849
Fragments arranged by size:
1849 261 5
MscI TGG'CCA
Cuts at: 0 43 1301 2115
Size: 43 1258 814
Fragments arranged by size:
1258 814 43
MslI CAynn'nnrTG
Cuts at: 0 470 1156 1196 2115
Size: 470 686 40 919
Fragments arranged by size:
919 686 470 40
MspA1I CmG'CkG
Cuts at: 0 178 274 323 1269 1498 1871 1885 1905
Size: 178 96 49 946 229 373 14 20
Cuts at: 1905 1951 2115
Size: 46 164
Fragments arranged by size:
946 373 229 178 164 96 49 46
20 14
NaeI GCC'GGC
Cuts at: 0 1794 2115
Size: 1794 321
NarI GG'CG_CC
Cuts at: 0 15 1624 2115
Size: 15 1609 491
Fragments arranged by size:
1609 491 15
NcoI C'CATG_G
Cuts at: 0 405 1296 2115
Size: 405 891 819
Fragments arranged by size:
891 819 405
NgoMIV G'CCGG_C
Cuts at: 0 1792 2115
Size: 1792 323
PasI CC'CwG_GG
Cuts at: 0 677 1315 1578 2115
Size: 677 638 263 537
Fragments arranged by size:
677 638 537 263
PfoI T'CCnGG_A
Cuts at: 0 863 2115
Size: 863 1252
PmlI CAC'GTG
Cuts at: 0 1076 2115
Size: 1076 1039
PpuMI rG'GwC_Cy
Cuts at: 0 158 206 1432 1822 1960 2115
Size: 158 48 1226 390 138 155
Fragments arranged by size:
1226 390 158 155 138 48
PshAI GACnn'nnGTC
Cuts at: 0 1702 2115
Size: 1702 413
PspOMI G'GGCC_C
Cuts at: 0 1319 1360 1674 1736 1764 1878 2115
Size: 1319 41 314 62 28 114 237
Fragments arranged by size:
1319 314 237 114 62 41 28
PstI C_TGCA'G
Cuts at: 0 77 617 1956 2115
Size: 77 540 1339 159
Fragments arranged by size:
1339 540 159 77
PvuII CAG'CTG
Cuts at: 0 274 1498 1905 2115
Size: 274 1224 407 210
Fragments arranged by size:
1224 407 274 210
SacI G_AGCT'C
Cuts at: 0 1226 2115
Size: 1226 889
SacII CC_GC'GG
Cuts at: 0 324 2115
Size: 324 1791
SanDI GG'GwC_CC
Cuts at: 0 1432 1822 2115
Size: 1432 390 293
Fragments arranged by size:
1432 390 293
SfcI C'TryA_G
Cuts at: 0 73 613 1952 2115
Size: 73 540 1339 163
Fragments arranged by size:
1339 540 163 73
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 187 1735 1793 2115
Size: 187 1548 58 322
Fragments arranged by size:
1548 322 187 58
SfoI GGC'GCC
Cuts at: 0 16 1625 2115
Size: 16 1609 490
Fragments arranged by size:
1609 490 16
SmaI CCC'GGG
Cuts at: 0 138 204 1620 1877 2115
Size: 138 66 1416 257 238
Fragments arranged by size:
1416 257 238 138 66
SrfI GCCC'GGGC
Cuts at: 0 1877 2115
Size: 1877 238
StuI AGG'CCT
Cuts at: 0 973 1484 1729 2115
Size: 973 511 245 386
Fragments arranged by size:
973 511 386 245
StyI C'CwwG_G
Cuts at: 0 405 1296 1328 1485 2115
Size: 405 891 32 157 630
Fragments arranged by size:
891 630 405 157 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 669 1056 1860 2115
Size: 669 387 804 255
Fragments arranged by size:
804 669 387 255
TatI w'GTAC_w
Cuts at: 0 781 2115
Size: 781 1334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 117 149 470 502 2115
Size: 117 32 321 32 1613
Fragments arranged by size:
1613 321 117 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1308 2115
Size: 1308 807
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 472 1902 2115
Size: 345 127 1430 213
Fragments arranged by size:
1430 345 213 127
XmaI C'CCGG_G
Cuts at: 0 136 202 1618 1875 2115
Size: 136 66 1416 257 240
Fragments arranged by size:
1416 257 240 136 66
XmnI GAAnn'nnTTC
Cuts at: 0 500 2092 2115
Size: 500 1592 23
Fragments arranged by size:
1592 500 23
ZraI GAC'GTC
Cuts at: 0 986 1891 2115
Size: 986 905 224
Fragments arranged by size:
986 905 224
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AlfI
AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HpaI KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PasI PfoI PmlI PpuMI PshAI PspOMI
PstI PvuII SacI SacII SanDI SfcI SfiI SfoI
SmaI SrfI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AleI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BglII
BmtI BpuEI BsaBI BsaWI BsaXI BseRI BsgI BsiWI
BspEI BspHI BsrGI BssHII BstAPI BstBI BstZ17I DraI
DraIII EcoRV FalI FseI FspAI HindIII KpnI MfeI
MluI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PpiI PsiI PspXI PsrI PvuI
RsrII SalI SapI SbfI ScaI SexAI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI SwaI XbaI XhoI