(Linear) (Six-base) MAPSORT of: KIEE2062.seq Check: 738 from: 1 to: 2463 KIEE2062.orf With 182 enzymes: SgfI * July 8, 2010 13:19 .. AccI GT'mk_AC Cuts at: 0 338 2036 2463 Size: 338 1698 427 Fragments arranged by size: 1698 427 338 AclI AA'CG_TT Cuts at: 0 2245 2463 Size: 2245 218 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 57 1209 2167 2357 2463 Size: 57 1152 958 190 106 Fragments arranged by size: 1152 958 190 106 57 AflIII A'CryG_T Cuts at: 0 725 2463 Size: 725 1738 AhdI GACnn_n'nnGTC Cuts at: 0 1809 2463 Size: 1809 654 AlwNI CAG_nnn'CTG Cuts at: 0 1087 2463 Size: 1087 1376 ApaI G_GGCC'C Cuts at: 0 242 908 2463 Size: 242 666 1555 Fragments arranged by size: 1555 666 242 ApaLI G'TGCA_C Cuts at: 0 229 1243 2463 Size: 229 1014 1220 Fragments arranged by size: 1220 1014 229 ApoI r'AATT_y Cuts at: 0 2276 2463 Size: 2276 187 AvaI C'yCGr_G Cuts at: 0 1744 2463 Size: 1744 719 BanI G'GyrC_C Cuts at: 0 286 2463 Size: 286 2177 BanII G_rGCy'C Cuts at: 0 242 908 1734 2104 2463 Size: 242 666 826 370 359 Fragments arranged by size: 826 666 370 359 242 BbeI G_GCGC'C Cuts at: 0 290 2463 Size: 290 2173 BbsI GAAGACnn'nnnn_ Cuts at: 0 1146 2270 2463 Size: 1146 1124 193 Fragments arranged by size: 1146 1124 193 BbvCI CC'TCA_GC Cuts at: 0 2314 2463 Size: 2314 149 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 586 620 2463 Size: 586 34 1843 Fragments arranged by size: 1843 586 34 BciVI GTATCCnnnnn_n' Cuts at: 0 81 1273 2444 2463 Size: 81 1192 1171 19 Fragments arranged by size: 1192 1171 81 19 BclI T'GATC_A Cuts at: 0 206 2463 Size: 206 2257 BglI GCCn_nnn'nGGC Cuts at: 0 762 912 2463 Size: 762 150 1551 Fragments arranged by size: 1551 762 150 BglII A'GATC_T Cuts at: 0 2084 2463 Size: 2084 379 Bme1580I G_kGCm'C Cuts at: 0 233 242 908 1247 2231 2463 Size: 233 9 666 339 984 232 Fragments arranged by size: 984 666 339 233 232 9 BmrI ACTGGGnnnn_n' Cuts at: 0 1456 2463 Size: 1456 1007 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1320 1716 1741 2463 Size: 1320 396 25 722 Fragments arranged by size: 1320 722 396 25 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 660 1147 1997 2381 2463 Size: 660 487 850 384 82 Fragments arranged by size: 850 660 487 384 82 Bpu10I CC'TnA_GC Cuts at: 0 2314 2463 Size: 2314 149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 910 919 2463 Size: 910 9 1544 Fragments arranged by size: 1544 910 9 BsaI GGTCTCn'nnnn_ Cuts at: 0 1392 2232 2463 Size: 1392 840 231 Fragments arranged by size: 1392 840 231 BsaAI yAC'GTr Cuts at: 0 150 728 879 1022 2463 Size: 150 578 151 143 1441 Fragments arranged by size: 1441 578 151 150 143 BsaHI Gr'CG_yC Cuts at: 0 287 2463 Size: 287 2176 BsaWI w'CCGG_w Cuts at: 0 388 2463 Size: 388 2075 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 803 833 2463 Size: 803 30 1630 Fragments arranged by size: 1630 803 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 827 1296 1798 2463 Size: 827 469 502 665 Fragments arranged by size: 827 665 502 469 BseYI C'CCAG_C Cuts at: 0 8 537 851 1009 2463 Size: 8 529 314 158 1454 Fragments arranged by size: 1454 529 314 158 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1644 2166 2463 Size: 1644 522 297 Fragments arranged by size: 1644 522 297 BsiEI CG_ry'CG Cuts at: 0 754 2463 Size: 754 1709 BsiHKAI G_wGCw'C Cuts at: 0 233 459 1247 2463 Size: 233 226 788 1216 Fragments arranged by size: 1216 788 233 226 BsmBI CGTCTCn'nnnn_ Cuts at: 0 809 2463 Size: 809 1654 Bsp1286I G_dGCh'C Cuts at: 0 233 242 459 908 1247 1734 2104 2231 Size: 233 9 217 449 339 487 370 127 Cuts at: 2231 2463 Size: 232 Fragments arranged by size: 487 449 370 339 233 232 217 127 9 BspEI T'CCGG_A Cuts at: 0 388 2463 Size: 388 2075 BsrFI r'CCGG_y Cuts at: 0 761 786 943 1236 2463 Size: 761 25 157 293 1227 Fragments arranged by size: 1227 761 293 157 25 BsrGI T'GTAC_A Cuts at: 0 2195 2463 Size: 2195 268 BstAPI GCAn_nnn'nTGC Cuts at: 0 2226 2463 Size: 2226 237 BstEII G'GTnAC_C Cuts at: 0 36 1533 2463 Size: 36 1497 930 Fragments arranged by size: 1497 930 36 BstXI CCAn_nnnn'nTGG Cuts at: 0 1839 2003 2463 Size: 1839 164 460 Fragments arranged by size: 1839 460 164 BstYI r'GATC_y Cuts at: 0 2084 2463 Size: 2084 379 BstZ17I GTA'TAC Cuts at: 0 2037 2463 Size: 2037 426 Bsu36I CC'TnA_GG Cuts at: 0 1781 2463 Size: 1781 682 BtgI C'CryG_G Cuts at: 0 41 956 1538 2123 2463 Size: 41 915 582 585 340 Fragments arranged by size: 915 585 582 340 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 589 2463 Size: 589 1874 BtsI GCAGTG_nn' Cuts at: 0 1505 2231 2457 2463 Size: 1505 726 226 6 Fragments arranged by size: 1505 726 226 6 EaeI y'GGCC_r Cuts at: 0 512 1541 1829 2463 Size: 512 1029 288 634 Fragments arranged by size: 1029 634 512 288 EarI CTCTTCn'nnn_ Cuts at: 0 101 1086 1359 2463 Size: 101 985 273 1104 Fragments arranged by size: 1104 985 273 101 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1507 2463 Size: 1507 956 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 57 660 1147 1209 1997 2167 2357 2381 Size: 57 603 487 62 788 170 190 24 Cuts at: 2381 2463 Size: 82 Fragments arranged by size: 788 603 487 190 170 82 62 57 24 EcoO109I rG'GnC_Cy Cuts at: 0 238 1410 2463 Size: 238 1172 1053 Fragments arranged by size: 1172 1053 238 EcoRI G'AATT_C Cuts at: 0 2276 2463 Size: 2276 187 EcoRV GAT'ATC Cuts at: 0 1049 1950 2463 Size: 1049 901 513 Fragments arranged by size: 1049 901 513 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1239 2381 2463 Size: 1239 1142 82 Fragments arranged by size: 1239 1142 82 HaeII r_GCGC'y Cuts at: 0 290 1087 2463 Size: 290 797 1376 Fragments arranged by size: 1376 797 290 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 367 399 1579 1611 1795 1827 2044 2076 Size: 367 32 1180 32 184 32 217 32 Cuts at: 2076 2463 Size: 387 Fragments arranged by size: 1180 387 367 217 184 32 32 32 32 HincII GTy'rAC Cuts at: 0 951 1070 2463 Size: 951 119 1393 Fragments arranged by size: 1393 951 119 HpaI GTT'AAC Cuts at: 0 951 2463 Size: 951 1512 KasI G'GCGC_C Cuts at: 0 286 2463 Size: 286 2177 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 193 789 1343 2463 Size: 193 596 554 1120 Fragments arranged by size: 1120 596 554 193 MscI TGG'CCA Cuts at: 0 1831 2463 Size: 1831 632 MslI CAynn'nnrTG Cuts at: 0 737 1372 2048 2203 2463 Size: 737 635 676 155 260 Fragments arranged by size: 737 676 635 260 155 MspA1I CmG'CkG Cuts at: 0 8 1329 1827 2463 Size: 8 1321 498 636 Fragments arranged by size: 1321 636 498 8 NaeI GCC'GGC Cuts at: 0 788 1238 2463 Size: 788 450 1225 Fragments arranged by size: 1225 788 450 NarI GG'CG_CC Cuts at: 0 287 2463 Size: 287 2176 NcoI C'CATG_G Cuts at: 0 41 2463 Size: 41 2422 NgoMIV G'CCGG_C Cuts at: 0 786 1236 2463 Size: 786 450 1227 Fragments arranged by size: 1227 786 450 NspI r_CATG'y Cuts at: 0 2224 2463 Size: 2224 239 PasI CC'CwG_GG Cuts at: 0 1458 2113 2463 Size: 1458 655 350 Fragments arranged by size: 1458 655 350 PflMI CCAn_nnn'nTGG Cuts at: 0 569 1087 2463 Size: 569 518 1376 Fragments arranged by size: 1376 569 518 PmlI CAC'GTG Cuts at: 0 879 2463 Size: 879 1584 PpuMI rG'GwC_Cy Cuts at: 0 1410 2463 Size: 1410 1053 PspOMI G'GGCC_C Cuts at: 0 238 904 2463 Size: 238 666 1559 Fragments arranged by size: 1559 666 238 PvuI CG_AT'CG Cuts at: 0 754 2463 Size: 754 1709 PvuII CAG'CTG Cuts at: 0 8 1827 2463 Size: 8 1819 636 Fragments arranged by size: 1819 636 8 SapI GCTCTTCn'nnn_ Cuts at: 0 101 2463 Size: 101 2362 ScaI AGT'ACT Cuts at: 0 271 1756 1846 2463 Size: 271 1485 90 617 Fragments arranged by size: 1485 617 271 90 SfcI C'TryA_G Cuts at: 0 335 2463 Size: 335 2128 SfoI GGC'GCC Cuts at: 0 288 2463 Size: 288 2175 SgrAI Cr'CCGG_yG Cuts at: 0 943 2463 Size: 943 1520 SmaI CCC'GGG Cuts at: 0 1746 2463 Size: 1746 717 SmlI C'TyrA_G Cuts at: 0 925 934 2463 Size: 925 9 1529 Fragments arranged by size: 1529 925 9 SphI G_CATG'C Cuts at: 0 2224 2463 Size: 2224 239 StyI C'CwwG_G Cuts at: 0 41 1523 2463 Size: 41 1482 940 Fragments arranged by size: 1482 940 41 TatI w'GTAC_w Cuts at: 0 269 319 1754 1844 2195 2463 Size: 269 50 1435 90 351 268 Fragments arranged by size: 1435 351 269 268 90 50 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1006 1038 2463 Size: 1006 32 1425 Fragments arranged by size: 1425 1006 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 566 1326 1893 2463 Size: 566 760 567 570 Fragments arranged by size: 760 570 567 566 XmaI C'CCGG_G Cuts at: 0 1744 2463 Size: 1744 719 Enzymes that do cut: AccI AclI AcuI AflIII AhdI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrFI BsrGI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HpaI KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PmlI PpuMI PspOMI PvuI PvuII SapI ScaI SfcI SfoI SgrAI SmaI SmlI SphI StyI TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AgeI AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI BfrBI BlpI BmgBI BmtI BsaBI BsiWI BsmI BspHI BspMI BsrBI BsrDI BssHII BssSI BstBI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI FseI FspI FspAI HindIII KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SgfI SnaBI SpeI SrfI SspI StuI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI