Restriction map for
pF1KE2062
(Linear) (Six-base) MAPSORT of: KIEE2062.seq Check: 738 from: 1 to: 2463
KIEE2062.orf
With 182 enzymes: SgfI *
July 8, 2010 13:19 ..
AccI GT'mk_AC
Cuts at: 0 338 2036 2463
Size: 338 1698 427
Fragments arranged by size:
1698 427 338
AclI AA'CG_TT
Cuts at: 0 2245 2463
Size: 2245 218
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 1209 2167 2357 2463
Size: 57 1152 958 190 106
Fragments arranged by size:
1152 958 190 106 57
AflIII A'CryG_T
Cuts at: 0 725 2463
Size: 725 1738
AhdI GACnn_n'nnGTC
Cuts at: 0 1809 2463
Size: 1809 654
AlwNI CAG_nnn'CTG
Cuts at: 0 1087 2463
Size: 1087 1376
ApaI G_GGCC'C
Cuts at: 0 242 908 2463
Size: 242 666 1555
Fragments arranged by size:
1555 666 242
ApaLI G'TGCA_C
Cuts at: 0 229 1243 2463
Size: 229 1014 1220
Fragments arranged by size:
1220 1014 229
ApoI r'AATT_y
Cuts at: 0 2276 2463
Size: 2276 187
AvaI C'yCGr_G
Cuts at: 0 1744 2463
Size: 1744 719
BanI G'GyrC_C
Cuts at: 0 286 2463
Size: 286 2177
BanII G_rGCy'C
Cuts at: 0 242 908 1734 2104 2463
Size: 242 666 826 370 359
Fragments arranged by size:
826 666 370 359 242
BbeI G_GCGC'C
Cuts at: 0 290 2463
Size: 290 2173
BbsI GAAGACnn'nnnn_
Cuts at: 0 1146 2270 2463
Size: 1146 1124 193
Fragments arranged by size:
1146 1124 193
BbvCI CC'TCA_GC
Cuts at: 0 2314 2463
Size: 2314 149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 586 620 2463
Size: 586 34 1843
Fragments arranged by size:
1843 586 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 81 1273 2444 2463
Size: 81 1192 1171 19
Fragments arranged by size:
1192 1171 81 19
BclI T'GATC_A
Cuts at: 0 206 2463
Size: 206 2257
BglI GCCn_nnn'nGGC
Cuts at: 0 762 912 2463
Size: 762 150 1551
Fragments arranged by size:
1551 762 150
BglII A'GATC_T
Cuts at: 0 2084 2463
Size: 2084 379
Bme1580I G_kGCm'C
Cuts at: 0 233 242 908 1247 2231 2463
Size: 233 9 666 339 984 232
Fragments arranged by size:
984 666 339 233 232 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 1456 2463
Size: 1456 1007
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1320 1716 1741 2463
Size: 1320 396 25 722
Fragments arranged by size:
1320 722 396 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 1147 1997 2381 2463
Size: 660 487 850 384 82
Fragments arranged by size:
850 660 487 384 82
Bpu10I CC'TnA_GC
Cuts at: 0 2314 2463
Size: 2314 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 919 2463
Size: 910 9 1544
Fragments arranged by size:
1544 910 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 1392 2232 2463
Size: 1392 840 231
Fragments arranged by size:
1392 840 231
BsaAI yAC'GTr
Cuts at: 0 150 728 879 1022 2463
Size: 150 578 151 143 1441
Fragments arranged by size:
1441 578 151 150 143
BsaHI Gr'CG_yC
Cuts at: 0 287 2463
Size: 287 2176
BsaWI w'CCGG_w
Cuts at: 0 388 2463
Size: 388 2075
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 803 833 2463
Size: 803 30 1630
Fragments arranged by size:
1630 803 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 827 1296 1798 2463
Size: 827 469 502 665
Fragments arranged by size:
827 665 502 469
BseYI C'CCAG_C
Cuts at: 0 8 537 851 1009 2463
Size: 8 529 314 158 1454
Fragments arranged by size:
1454 529 314 158 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1644 2166 2463
Size: 1644 522 297
Fragments arranged by size:
1644 522 297
BsiEI CG_ry'CG
Cuts at: 0 754 2463
Size: 754 1709
BsiHKAI G_wGCw'C
Cuts at: 0 233 459 1247 2463
Size: 233 226 788 1216
Fragments arranged by size:
1216 788 233 226
BsmBI CGTCTCn'nnnn_
Cuts at: 0 809 2463
Size: 809 1654
Bsp1286I G_dGCh'C
Cuts at: 0 233 242 459 908 1247 1734 2104 2231
Size: 233 9 217 449 339 487 370 127
Cuts at: 2231 2463
Size: 232
Fragments arranged by size:
487 449 370 339 233 232 217 127
9
BspEI T'CCGG_A
Cuts at: 0 388 2463
Size: 388 2075
BsrFI r'CCGG_y
Cuts at: 0 761 786 943 1236 2463
Size: 761 25 157 293 1227
Fragments arranged by size:
1227 761 293 157 25
BsrGI T'GTAC_A
Cuts at: 0 2195 2463
Size: 2195 268
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2226 2463
Size: 2226 237
BstEII G'GTnAC_C
Cuts at: 0 36 1533 2463
Size: 36 1497 930
Fragments arranged by size:
1497 930 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1839 2003 2463
Size: 1839 164 460
Fragments arranged by size:
1839 460 164
BstYI r'GATC_y
Cuts at: 0 2084 2463
Size: 2084 379
BstZ17I GTA'TAC
Cuts at: 0 2037 2463
Size: 2037 426
Bsu36I CC'TnA_GG
Cuts at: 0 1781 2463
Size: 1781 682
BtgI C'CryG_G
Cuts at: 0 41 956 1538 2123 2463
Size: 41 915 582 585 340
Fragments arranged by size:
915 585 582 340 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 589 2463
Size: 589 1874
BtsI GCAGTG_nn'
Cuts at: 0 1505 2231 2457 2463
Size: 1505 726 226 6
Fragments arranged by size:
1505 726 226 6
EaeI y'GGCC_r
Cuts at: 0 512 1541 1829 2463
Size: 512 1029 288 634
Fragments arranged by size:
1029 634 512 288
EarI CTCTTCn'nnn_
Cuts at: 0 101 1086 1359 2463
Size: 101 985 273 1104
Fragments arranged by size:
1104 985 273 101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1507 2463
Size: 1507 956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 660 1147 1209 1997 2167 2357 2381
Size: 57 603 487 62 788 170 190 24
Cuts at: 2381 2463
Size: 82
Fragments arranged by size:
788 603 487 190 170 82 62 57
24
EcoO109I rG'GnC_Cy
Cuts at: 0 238 1410 2463
Size: 238 1172 1053
Fragments arranged by size:
1172 1053 238
EcoRI G'AATT_C
Cuts at: 0 2276 2463
Size: 2276 187
EcoRV GAT'ATC
Cuts at: 0 1049 1950 2463
Size: 1049 901 513
Fragments arranged by size:
1049 901 513
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1239 2381 2463
Size: 1239 1142 82
Fragments arranged by size:
1239 1142 82
HaeII r_GCGC'y
Cuts at: 0 290 1087 2463
Size: 290 797 1376
Fragments arranged by size:
1376 797 290
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 367 399 1579 1611 1795 1827 2044 2076
Size: 367 32 1180 32 184 32 217 32
Cuts at: 2076 2463
Size: 387
Fragments arranged by size:
1180 387 367 217 184 32 32 32
32
HincII GTy'rAC
Cuts at: 0 951 1070 2463
Size: 951 119 1393
Fragments arranged by size:
1393 951 119
HpaI GTT'AAC
Cuts at: 0 951 2463
Size: 951 1512
KasI G'GCGC_C
Cuts at: 0 286 2463
Size: 286 2177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 789 1343 2463
Size: 193 596 554 1120
Fragments arranged by size:
1120 596 554 193
MscI TGG'CCA
Cuts at: 0 1831 2463
Size: 1831 632
MslI CAynn'nnrTG
Cuts at: 0 737 1372 2048 2203 2463
Size: 737 635 676 155 260
Fragments arranged by size:
737 676 635 260 155
MspA1I CmG'CkG
Cuts at: 0 8 1329 1827 2463
Size: 8 1321 498 636
Fragments arranged by size:
1321 636 498 8
NaeI GCC'GGC
Cuts at: 0 788 1238 2463
Size: 788 450 1225
Fragments arranged by size:
1225 788 450
NarI GG'CG_CC
Cuts at: 0 287 2463
Size: 287 2176
NcoI C'CATG_G
Cuts at: 0 41 2463
Size: 41 2422
NgoMIV G'CCGG_C
Cuts at: 0 786 1236 2463
Size: 786 450 1227
Fragments arranged by size:
1227 786 450
NspI r_CATG'y
Cuts at: 0 2224 2463
Size: 2224 239
PasI CC'CwG_GG
Cuts at: 0 1458 2113 2463
Size: 1458 655 350
Fragments arranged by size:
1458 655 350
PflMI CCAn_nnn'nTGG
Cuts at: 0 569 1087 2463
Size: 569 518 1376
Fragments arranged by size:
1376 569 518
PmlI CAC'GTG
Cuts at: 0 879 2463
Size: 879 1584
PpuMI rG'GwC_Cy
Cuts at: 0 1410 2463
Size: 1410 1053
PspOMI G'GGCC_C
Cuts at: 0 238 904 2463
Size: 238 666 1559
Fragments arranged by size:
1559 666 238
PvuI CG_AT'CG
Cuts at: 0 754 2463
Size: 754 1709
PvuII CAG'CTG
Cuts at: 0 8 1827 2463
Size: 8 1819 636
Fragments arranged by size:
1819 636 8
SapI GCTCTTCn'nnn_
Cuts at: 0 101 2463
Size: 101 2362
ScaI AGT'ACT
Cuts at: 0 271 1756 1846 2463
Size: 271 1485 90 617
Fragments arranged by size:
1485 617 271 90
SfcI C'TryA_G
Cuts at: 0 335 2463
Size: 335 2128
SfoI GGC'GCC
Cuts at: 0 288 2463
Size: 288 2175
SgrAI Cr'CCGG_yG
Cuts at: 0 943 2463
Size: 943 1520
SmaI CCC'GGG
Cuts at: 0 1746 2463
Size: 1746 717
SmlI C'TyrA_G
Cuts at: 0 925 934 2463
Size: 925 9 1529
Fragments arranged by size:
1529 925 9
SphI G_CATG'C
Cuts at: 0 2224 2463
Size: 2224 239
StyI C'CwwG_G
Cuts at: 0 41 1523 2463
Size: 41 1482 940
Fragments arranged by size:
1482 940 41
TatI w'GTAC_w
Cuts at: 0 269 319 1754 1844 2195 2463
Size: 269 50 1435 90 351 268
Fragments arranged by size:
1435 351 269 268 90 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1006 1038 2463
Size: 1006 32 1425
Fragments arranged by size:
1425 1006 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 566 1326 1893 2463
Size: 566 760 567 570
Fragments arranged by size:
760 570 567 566
XmaI C'CCGG_G
Cuts at: 0 1744 2463
Size: 1744 719
Enzymes that do cut:
AccI AclI AcuI AflIII AhdI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrFI
BsrGI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HpaI KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PmlI PpuMI PspOMI PvuI PvuII SapI
ScaI SfcI SfoI SgrAI SmaI SmlI SphI StyI
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AleI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BfrBI
BlpI BmgBI BmtI BsaBI BsiWI BsmI BspHI BspMI
BsrBI BsrDI BssHII BssSI BstBI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoNI FseI FspI FspAI
HindIII KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PciI PfoI PmeI PpiI PshAI PsiI
PspXI PsrI PstI RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SgfI SnaBI SpeI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI