Restriction map for
pF1KE2076
(Linear) (Six-base) MAPSORT of: KIEE2076.seq Check: 6567 from: 1 to: 2235
KIEE2076.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 0 1656 2235
Size: 1656 579
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 439 1304 1493 1709 2042 2099 2235
Size: 260 179 865 189 216 333 57 136
Fragments arranged by size:
865 333 260 216 189 179 136 57
AlwNI CAG_nnn'CTG
Cuts at: 0 561 601 2235
Size: 561 40 1634
Fragments arranged by size:
1634 561 40
ApaI G_GGCC'C
Cuts at: 0 41 2235
Size: 41 2194
ApaLI G'TGCA_C
Cuts at: 0 1291 1771 2235
Size: 1291 480 464
Fragments arranged by size:
1291 480 464
ApoI r'AATT_y
Cuts at: 0 924 1076 2136 2235
Size: 924 152 1060 99
Fragments arranged by size:
1060 924 152 99
AseI AT'TA_AT
Cuts at: 0 907 2235
Size: 907 1328
AvaI C'yCGr_G
Cuts at: 0 12 2235
Size: 12 2223
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 541 574 2235
Size: 541 33 1661
Fragments arranged by size:
1661 541 33
BamHI G'GATC_C
Cuts at: 0 711 1086 2002 2235
Size: 711 375 916 233
Fragments arranged by size:
916 711 375 233
BanI G'GyrC_C
Cuts at: 0 64 173 2235
Size: 64 109 2062
Fragments arranged by size:
2062 109 64
BanII G_rGCy'C
Cuts at: 0 41 575 1642 2235
Size: 41 534 1067 593
Fragments arranged by size:
1067 593 534 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 991 1322 1354 1656 2235
Size: 991 331 32 302 579
Fragments arranged by size:
991 579 331 302 32
BbvCI CC'TCA_GC
Cuts at: 0 851 1565 2235
Size: 851 714 670
Fragments arranged by size:
851 714 670
BciVI GTATCCnnnnn_n'
Cuts at: 0 1576 2235
Size: 1576 659
BclI T'GATC_A
Cuts at: 0 516 2070 2235
Size: 516 1554 165
Fragments arranged by size:
1554 516 165
BglI GCCn_nnn'nGGC
Cuts at: 0 15 2235
Size: 15 2220
BglII A'GATC_T
Cuts at: 0 303 429 2235
Size: 303 126 1806
Fragments arranged by size:
1806 303 126
Bme1580I G_kGCm'C
Cuts at: 0 41 67 1295 1775 2235
Size: 41 26 1228 480 460
Fragments arranged by size:
1228 480 460 41 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 322 2235
Size: 322 1913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 1609 2235
Size: 317 1292 626
Fragments arranged by size:
1292 626 317
Bpu10I CC'TnA_GC
Cuts at: 0 851 1565 2235
Size: 851 714 670
Fragments arranged by size:
851 714 670
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1786 2084 2235
Size: 1037 749 298 151
Fragments arranged by size:
1037 749 298 151
BsaBI GATnn'nnATC
Cuts at: 0 515 2235
Size: 515 1720
BsaHI Gr'CG_yC
Cuts at: 0 1653 2235
Size: 1653 582
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 854 884 2235
Size: 854 30 1351
Fragments arranged by size:
1351 854 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 314 875 2087 2235
Size: 314 561 1212 148
Fragments arranged by size:
1212 561 314 148
BseYI C'CCAG_C
Cuts at: 0 60 2235
Size: 60 2175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1801 2039 2235
Size: 1801 238 196
Fragments arranged by size:
1801 238 196
BsiEI CG_ry'CG
Cuts at: 0 25 2235
Size: 25 2210
BsiHKAI G_wGCw'C
Cuts at: 0 575 1295 1775 1802 2235
Size: 575 720 480 27 433
Fragments arranged by size:
720 575 480 433 27
BsmI GAATG_Cn'
Cuts at: 0 1457 2235
Size: 1457 778
Bsp1286I G_dGCh'C
Cuts at: 0 41 67 575 1295 1642 1775 1802 2235
Size: 41 26 508 720 347 133 27 433
Fragments arranged by size:
720 508 433 347 133 41 27 26
BspHI T'CATG_A
Cuts at: 0 261 2172 2235
Size: 261 1911 63
Fragments arranged by size:
1911 261 63
BsrBI CCG'CTC
Cuts at: 0 6 57 2235
Size: 6 51 2178
Fragments arranged by size:
2178 51 6
BsrDI GCAATG_nn'
Cuts at: 0 287 1358 2235
Size: 287 1071 877
Fragments arranged by size:
1071 877 287
BsrFI r'CCGG_y
Cuts at: 0 67 2235
Size: 67 2168
BsrGI T'GTAC_A
Cuts at: 0 532 1230 1972 2235
Size: 532 698 742 263
Fragments arranged by size:
742 698 532 263
BstAPI GCAn_nnn'nTGC
Cuts at: 0 672 1779 2235
Size: 672 1107 456
Fragments arranged by size:
1107 672 456
BstXI CCAn_nnnn'nTGG
Cuts at: 0 686 2235
Size: 686 1549
BstYI r'GATC_y
Cuts at: 0 303 429 711 1086 2002 2235
Size: 303 126 282 375 916 233
Fragments arranged by size:
916 375 303 282 233 126
Bsu36I CC'TnA_GG
Cuts at: 0 1868 2235
Size: 1868 367
BtgI C'CryG_G
Cuts at: 0 626 812 2235
Size: 626 186 1423
Fragments arranged by size:
1423 626 186
BtsI GCAGTG_nn'
Cuts at: 0 550 1295 2235
Size: 550 745 940
Fragments arranged by size:
940 745 550
DraIII CAC_nnn'GTG
Cuts at: 0 1299 1779 2235
Size: 1299 480 456
Fragments arranged by size:
1299 480 456
DrdI GACnn_nn'nnGTC
Cuts at: 0 1912 2235
Size: 1912 323
EaeI y'GGCC_r
Cuts at: 0 22 2151 2235
Size: 22 2129 84
Fragments arranged by size:
2129 84 22
EagI C'GGCC_G
Cuts at: 0 22 2235
Size: 22 2213
EarI CTCTTCn'nnn_
Cuts at: 0 278 731 1630 2235
Size: 278 453 899 605
Fragments arranged by size:
899 605 453 278
EcoICRI GAG'CTC
Cuts at: 0 573 2235
Size: 573 1662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 317 439 1304 1493 1609 1709 2042
Size: 260 57 122 865 189 116 100 333
Cuts at: 2042 2099 2235
Size: 57 136
Fragments arranged by size:
865 333 260 189 136 122 116 100
57 57
EcoO109I rG'GnC_Cy
Cuts at: 0 38 846 865 2235
Size: 38 808 19 1370
Fragments arranged by size:
1370 808 38 19
EcoRV GAT'ATC
Cuts at: 0 1505 2235
Size: 1505 730
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1078 2235
Size: 1078 1157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 1659 1691 1842 1874 2235
Size: 370 32 1257 32 151 32 361
Fragments arranged by size:
1257 370 361 151 32 32 32
HincII GTy'rAC
Cuts at: 0 873 2228 2235
Size: 873 1355 7
Fragments arranged by size:
1355 873 7
HpaI GTT'AAC
Cuts at: 0 2228 2235
Size: 2228 7
MscI TGG'CCA
Cuts at: 0 2153 2235
Size: 2153 82
MslI CAynn'nnrTG
Cuts at: 0 212 1753 1921 2040 2235
Size: 212 1541 168 119 195
Fragments arranged by size:
1541 212 195 168 119
MspA1I CmG'CkG
Cuts at: 0 1266 2235
Size: 1266 969
NcoI C'CATG_G
Cuts at: 0 626 812 2235
Size: 626 186 1423
Fragments arranged by size:
1423 626 186
NspI r_CATG'y
Cuts at: 0 734 1615 2235
Size: 734 881 620
Fragments arranged by size:
881 734 620
PasI CC'CwG_GG
Cuts at: 0 41 2235
Size: 41 2194
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 98 130 2235
Size: 98 32 2105
Fragments arranged by size:
2105 98 32
PpuMI rG'GwC_Cy
Cuts at: 0 846 865 2235
Size: 846 19 1370
Fragments arranged by size:
1370 846 19
PspOMI G'GGCC_C
Cuts at: 0 37 2235
Size: 37 2198
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1965 1997 2235
Size: 1965 32 238
Fragments arranged by size:
1965 238 32
PstI C_TGCA'G
Cuts at: 0 557 677 2235
Size: 557 120 1558
Fragments arranged by size:
1558 557 120
PvuII CAG'CTG
Cuts at: 0 1266 2235
Size: 1266 969
SacI G_AGCT'C
Cuts at: 0 575 2235
Size: 575 1660
SanDI GG'GwC_CC
Cuts at: 0 846 2235
Size: 846 1389
SapI GCTCTTCn'nnn_
Cuts at: 0 1630 2235
Size: 1630 605
SfcI C'TryA_G
Cuts at: 0 553 673 826 1009 1320 1630 1737 2235
Size: 553 120 153 183 311 310 107 498
Fragments arranged by size:
553 498 311 310 183 153 120 107
SgrAI Cr'CCGG_yG
Cuts at: 0 67 2235
Size: 67 2168
SmaI CCC'GGG
Cuts at: 0 14 2235
Size: 14 2221
SmlI C'TyrA_G
Cuts at: 0 1052 1801 2099 2235
Size: 1052 749 298 136
Fragments arranged by size:
1052 749 298 136
SphI G_CATG'C
Cuts at: 0 1615 2235
Size: 1615 620
SspI AAT'ATT
Cuts at: 0 252 2235
Size: 252 1983
StuI AGG'CCT
Cuts at: 0 226 657 2235
Size: 226 431 1578
Fragments arranged by size:
1578 431 226
StyI C'CwwG_G
Cuts at: 0 626 812 2148 2235
Size: 626 186 1336 87
Fragments arranged by size:
1336 626 186 87
TatI w'GTAC_w
Cuts at: 0 532 936 1230 1972 2235
Size: 532 404 294 742 263
Fragments arranged by size:
742 532 404 294 263
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 703 735 2235
Size: 703 32 1500
Fragments arranged by size:
1500 703 32
XbaI T'CTAG_A
Cuts at: 0 1116 2010 2235
Size: 1116 894 225
Fragments arranged by size:
1116 894 225
XmaI C'CCGG_G
Cuts at: 0 12 2235
Size: 12 2223
XmnI GAAnn'nnTTC
Cuts at: 0 998 2235
Size: 998 1237
ZraI GAC'GTC
Cuts at: 0 1654 2235
Size: 1654 581
Enzymes that do cut:
AatII AcuI AlwNI ApaI ApaLI ApoI AseI AvaI
BaeI BamHI BanI BanII BbsI BbvCI BciVI BclI
BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I HincII
HpaI MscI MslI MspA1I NcoI NspI PasI PpiI
PpuMI PspOMI PsrI PstI PvuII SacI SanDI SapI
SfcI SgrAI SmaI SmlI SphI SspI StuI StyI
TatI TstI XbaI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AscI AsiSI AvrII BbeI
BcgI BfrBI BlpI BmgBI BmrI BmtI BsaI BsaAI
BsaWI BsiWI BsmBI BspEI BspMI BssHII BssSI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI EciI EcoNI
EcoRI FseI FspI FspAI HaeII HindIII KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PshAI PsiI PspXI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SnaBI SpeI
SrfI SwaI TaqII Tth111I XcmI XhoI