Restriction map for
pF1KE2078
(Linear) (Six-base) MAPSORT of: KIEE2078.seq Check: 9020 from: 1 to: 2244
KIEE2078.orf
With 182 enzymes: SgfI *
May 14, 2013 10:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 992 1839 2244
Size: 629 363 847 405
Fragments arranged by size:
847 629 405 363
AflIII A'CryG_T
Cuts at: 0 1741 2244
Size: 1741 503
AleI CACnn'nnGTG
Cuts at: 0 140 1680 1856 2244
Size: 140 1540 176 388
Fragments arranged by size:
1540 388 176 140
AlwNI CAG_nnn'CTG
Cuts at: 0 1636 1834 2244
Size: 1636 198 410
Fragments arranged by size:
1636 410 198
ApaLI G'TGCA_C
Cuts at: 0 1194 2244
Size: 1194 1050
ApoI r'AATT_y
Cuts at: 0 253 909 1215 1291 2244
Size: 253 656 306 76 953
Fragments arranged by size:
953 656 306 253 76
AseI AT'TA_AT
Cuts at: 0 1688 2244
Size: 1688 556
AvaI C'yCGr_G
Cuts at: 0 2210 2244
Size: 2210 34
BamHI G'GATC_C
Cuts at: 0 1168 1885 2244
Size: 1168 717 359
Fragments arranged by size:
1168 717 359
BanII G_rGCy'C
Cuts at: 0 1997 2244
Size: 1997 247
BbsI GAAGACnn'nnnn_
Cuts at: 0 173 210 942 2244
Size: 173 37 732 1302
Fragments arranged by size:
1302 732 173 37
BclI T'GATC_A
Cuts at: 0 684 2063 2244
Size: 684 1379 181
Fragments arranged by size:
1379 684 181
Bme1580I G_kGCm'C
Cuts at: 0 1198 2244
Size: 1198 1046
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2189 2244
Size: 2189 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1040 2244
Size: 1040 1204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 1351 2244
Size: 575 776 893
Fragments arranged by size:
893 776 575
BsaI GGTCTCn'nnnn_
Cuts at: 0 1112 2163 2244
Size: 1112 1051 81
Fragments arranged by size:
1112 1051 81
BsaAI yAC'GTr
Cuts at: 0 1742 2244
Size: 1742 502
BsaBI GATnn'nnATC
Cuts at: 0 1460 2244
Size: 1460 784
BsaWI w'CCGG_w
Cuts at: 0 787 2244
Size: 787 1457
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1726 1756 2244
Size: 1726 30 488
Fragments arranged by size:
1726 488 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 1747 2123 2178 2181 2244
Size: 19 1728 376 55 3 63
Fragments arranged by size:
1728 376 63 55 19 3
BseYI C'CCAG_C
Cuts at: 0 1629 2195 2244
Size: 1629 566 49
Fragments arranged by size:
1629 566 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1527 1721 1858 1953 2244
Size: 539 988 194 137 95 291
Fragments arranged by size:
988 539 291 194 137 95
BsiHKAI G_wGCw'C
Cuts at: 0 1005 1198 1997 2244
Size: 1005 193 799 247
Fragments arranged by size:
1005 799 247 193
BsmI GAATG_Cn'
Cuts at: 0 1450 1546 2244
Size: 1450 96 698
Fragments arranged by size:
1450 698 96
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2232 2244
Size: 2232 12
Bsp1286I G_dGCh'C
Cuts at: 0 1005 1198 1997 2244
Size: 1005 193 799 247
Fragments arranged by size:
1005 799 247 193
BspEI T'CCGG_A
Cuts at: 0 787 2244
Size: 787 1457
BspHI T'CATG_A
Cuts at: 0 468 924 2244
Size: 468 456 1320
Fragments arranged by size:
1320 468 456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1693 2244
Size: 1693 551
BsrDI GCAATG_nn'
Cuts at: 0 1702 2006 2244
Size: 1702 304 238
Fragments arranged by size:
1702 304 238
BsrGI T'GTAC_A
Cuts at: 0 1531 2244
Size: 1531 713
BssSI C'ACGA_G
Cuts at: 0 389 713 2244
Size: 389 324 1531
Fragments arranged by size:
1531 389 324
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1543 2244
Size: 1543 701
BstXI CCAn_nnnn'nTGG
Cuts at: 0 199 391 562 2244
Size: 199 192 171 1682
Fragments arranged by size:
1682 199 192 171
BstYI r'GATC_y
Cuts at: 0 1168 1885 2244
Size: 1168 717 359
Fragments arranged by size:
1168 717 359
Bsu36I CC'TnA_GG
Cuts at: 0 506 2244
Size: 506 1738
BtgI C'CryG_G
Cuts at: 0 198 345 2244
Size: 198 147 1899
Fragments arranged by size:
1899 198 147
BtsI GCAGTG_nn'
Cuts at: 0 1846 2103 2244
Size: 1846 257 141
Fragments arranged by size:
1846 257 141
DraI TTT'AAA
Cuts at: 0 74 258 699 2159 2244
Size: 74 184 441 1460 85
Fragments arranged by size:
1460 441 184 85 74
DraIII CAC_nnn'GTG
Cuts at: 0 1543 2244
Size: 1543 701
EaeI y'GGCC_r
Cuts at: 0 1365 2244
Size: 1365 879
EarI CTCTTCn'nnn_
Cuts at: 0 220 1333 2244
Size: 220 1113 911
Fragments arranged by size:
1113 911 220
EcoICRI GAG'CTC
Cuts at: 0 1995 2244
Size: 1995 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 992 1040 1839 2244
Size: 629 363 48 799 405
Fragments arranged by size:
799 629 405 363 48
EcoO109I rG'GnC_Cy
Cuts at: 0 852 1522 2244
Size: 852 670 722
Fragments arranged by size:
852 722 670
EcoRI G'AATT_C
Cuts at: 0 909 2244
Size: 909 1335
EcoRV GAT'ATC
Cuts at: 0 737 2244
Size: 737 1507
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1086 2244
Size: 1086 1158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 39 71 726 758 2010 2042 2244
Size: 39 32 655 32 1252 32 202
Fragments arranged by size:
1252 655 202 39 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 978 1134 2244
Size: 978 156 1110
Fragments arranged by size:
1110 978 156
MscI TGG'CCA
Cuts at: 0 1367 2244
Size: 1367 877
MslI CAynn'nnrTG
Cuts at: 0 140 197 320 473 1680 1856 2071 2244
Size: 140 57 123 153 1207 176 215 173
Fragments arranged by size:
1207 215 176 173 153 140 123 57
MspA1I CmG'CkG
Cuts at: 0 649 2205 2244
Size: 649 1556 39
Fragments arranged by size:
1556 649 39
NcoI C'CATG_G
Cuts at: 0 198 345 2244
Size: 198 147 1899
Fragments arranged by size:
1899 198 147
NspI r_CATG'y
Cuts at: 0 604 2244
Size: 604 1640
PflMI CCAn_nnn'nTGG
Cuts at: 0 883 2244
Size: 883 1361
PpuMI rG'GwC_Cy
Cuts at: 0 852 1522 2244
Size: 852 670 722
Fragments arranged by size:
852 722 670
PvuII CAG'CTG
Cuts at: 0 649 2244
Size: 649 1595
SacI G_AGCT'C
Cuts at: 0 1997 2244
Size: 1997 247
ScaI AGT'ACT
Cuts at: 0 281 2244
Size: 281 1963
SfcI C'TryA_G
Cuts at: 0 1302 1422 2244
Size: 1302 120 822
Fragments arranged by size:
1302 822 120
SmlI C'TyrA_G
Cuts at: 0 590 1330 2244
Size: 590 740 914
Fragments arranged by size:
914 740 590
SspI AAT'ATT
Cuts at: 0 703 882 2244
Size: 703 179 1362
Fragments arranged by size:
1362 703 179
StyI C'CwwG_G
Cuts at: 0 198 345 2244
Size: 198 147 1899
Fragments arranged by size:
1899 198 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 452 974 2244
Size: 452 522 1270
Fragments arranged by size:
1270 522 452
TatI w'GTAC_w
Cuts at: 0 279 1531 1825 2244
Size: 279 1252 294 419
Fragments arranged by size:
1252 419 294 279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1726 1758 2244
Size: 1726 32 486
Fragments arranged by size:
1726 486 32
XbaI T'CTAG_A
Cuts at: 0 183 2244
Size: 183 2061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 391 1375 2244
Size: 391 984 869
Fragments arranged by size:
984 869 391
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaLI ApoI AseI AvaI
BamHI BanII BbsI BclI Bme1580I BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI Hin4I MmeI MscI MslI MspA1I NcoI
NspI PflMI PpuMI PvuII SacI ScaI SfcI SmlI
SspI StyI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI AscI AsiSI AvrII BaeI
BanI BbeI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI Bpu10I BsaHI BsiEI BsiWI
BsrBI BsrFI BssHII BstBI BstEII BstZ17I BtgZI ClaI
CspCI DrdI EagI EciI EcoNI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XhoI XmaI XmnI ZraI