(Linear) (Six-base) MAPSORT of: KIEE2112.seq Check: 6796 from: 1 to: 2763 KIEE2112.orf With 182 enzymes: SgfI * February 10, 2017 14:54 .. AccI GT'mk_AC Cuts at: 0 2594 2763 Size: 2594 169 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 825 1482 1678 1853 2126 2404 2763 Size: 825 657 196 175 273 278 359 Fragments arranged by size: 825 657 359 278 273 196 175 AfeI AGC'GCT Cuts at: 0 343 2763 Size: 343 2420 AflIII A'CryG_T Cuts at: 0 2536 2763 Size: 2536 227 AlwNI CAG_nnn'CTG Cuts at: 0 642 1477 2763 Size: 642 835 1286 Fragments arranged by size: 1286 835 642 ApaI G_GGCC'C Cuts at: 0 292 2763 Size: 292 2471 ApaLI G'TGCA_C Cuts at: 0 745 2763 Size: 745 2018 ApoI r'AATT_y Cuts at: 0 1349 2140 2763 Size: 1349 791 623 Fragments arranged by size: 1349 791 623 AseI AT'TA_AT Cuts at: 0 2228 2763 Size: 2228 535 AvaI C'yCGr_G Cuts at: 0 855 2763 Size: 855 1908 AvrII C'CTAG_G Cuts at: 0 1600 2763 Size: 1600 1163 BanI G'GyrC_C Cuts at: 0 51 153 272 316 766 2763 Size: 51 102 119 44 450 1997 Fragments arranged by size: 1997 450 119 102 51 44 BanII G_rGCy'C Cuts at: 0 292 628 941 1586 2763 Size: 292 336 313 645 1177 Fragments arranged by size: 1177 645 336 313 292 BbeI G_GCGC'C Cuts at: 0 55 157 276 2763 Size: 55 102 119 2487 Fragments arranged by size: 2487 119 102 55 BbsI GAAGACnn'nnnn_ Cuts at: 0 74 2684 2763 Size: 74 2610 79 Fragments arranged by size: 2610 79 74 BciVI GTATCCnnnnn_n' Cuts at: 0 2152 2763 Size: 2152 611 BclI T'GATC_A Cuts at: 0 2133 2763 Size: 2133 630 BglI GCCn_nnn'nGGC Cuts at: 0 113 889 961 2763 Size: 113 776 72 1802 Fragments arranged by size: 1802 776 113 72 BglII A'GATC_T Cuts at: 0 1086 1493 1919 2763 Size: 1086 407 426 844 Fragments arranged by size: 1086 844 426 407 BlpI GC'TnA_GC Cuts at: 0 940 1861 2763 Size: 940 921 902 Fragments arranged by size: 940 921 902 Bme1580I G_kGCm'C Cuts at: 0 292 749 2763 Size: 292 457 2014 Fragments arranged by size: 2014 457 292 BmrI ACTGGGnnnn_n' Cuts at: 0 370 1509 2763 Size: 370 1139 1254 Fragments arranged by size: 1254 1139 370 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 165 1248 2763 Size: 165 1083 1515 Fragments arranged by size: 1515 1083 165 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 138 160 415 499 1129 2637 2763 Size: 138 22 255 84 630 1508 126 Fragments arranged by size: 1508 630 255 138 126 84 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 2472 2763 Size: 484 1988 291 Fragments arranged by size: 1988 484 291 BsaAI yAC'GTr Cuts at: 0 561 2763 Size: 561 2202 BsaHI Gr'CG_yC Cuts at: 0 52 125 154 273 353 530 2763 Size: 52 73 29 119 80 177 2233 Fragments arranged by size: 2233 177 119 80 73 52 29 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2313 2343 2763 Size: 2313 30 420 Fragments arranged by size: 2313 420 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 73 76 349 352 412 2763 Size: 73 3 273 3 60 2351 Fragments arranged by size: 2351 273 73 60 3 3 BseYI C'CCAG_C Cuts at: 0 1341 2743 2763 Size: 1341 1402 20 Fragments arranged by size: 1402 1341 20 BsiEI CG_ry'CG Cuts at: 0 784 2763 Size: 784 1979 BsiHKAI G_wGCw'C Cuts at: 0 476 749 941 1586 1862 2126 2763 Size: 476 273 192 645 276 264 637 Fragments arranged by size: 645 637 476 276 273 264 192 BsmI GAATG_Cn' Cuts at: 0 2519 2763 Size: 2519 244 BsmBI CGTCTCn'nnnn_ Cuts at: 0 234 619 2763 Size: 234 385 2144 Fragments arranged by size: 2144 385 234 Bsp1286I G_dGCh'C Cuts at: 0 292 476 628 749 941 1586 1862 2126 Size: 292 184 152 121 192 645 276 264 Cuts at: 2126 2763 Size: 637 Fragments arranged by size: 645 637 292 276 264 192 184 152 121 BsrBI CCG'CTC Cuts at: 0 780 2763 Size: 780 1983 BsrDI GCAATG_nn' Cuts at: 0 1018 1658 2763 Size: 1018 640 1105 Fragments arranged by size: 1105 1018 640 BsrFI r'CCGG_y Cuts at: 0 269 2763 Size: 269 2494 BssHII G'CGCG_C Cuts at: 0 517 2763 Size: 517 2246 BstAPI GCAn_nnn'nTGC Cuts at: 0 642 753 2763 Size: 642 111 2010 Fragments arranged by size: 2010 642 111 BstBI TT'CG_AA Cuts at: 0 1442 2763 Size: 1442 1321 BstEII G'GTnAC_C Cuts at: 0 39 483 2763 Size: 39 444 2280 Fragments arranged by size: 2280 444 39 BstXI CCAn_nnnn'nTGG Cuts at: 0 714 2763 Size: 714 2049 BstYI r'GATC_y Cuts at: 0 1041 1086 1493 1919 2763 Size: 1041 45 407 426 844 Fragments arranged by size: 1041 844 426 407 45 Bsu36I CC'TnA_GG Cuts at: 0 1240 2763 Size: 1240 1523 BtgI C'CryG_G Cuts at: 0 201 218 312 2763 Size: 201 17 94 2451 Fragments arranged by size: 2451 201 94 17 BtsI GCAGTG_nn' Cuts at: 0 723 2763 Size: 723 2040 DrdI GACnn_nn'nnGTC Cuts at: 0 1729 2763 Size: 1729 1034 EaeI y'GGCC_r Cuts at: 0 602 781 881 953 962 1307 2763 Size: 602 179 100 72 9 345 1456 Fragments arranged by size: 1456 602 345 179 100 72 9 EagI C'GGCC_G Cuts at: 0 781 2763 Size: 781 1982 EarI CTCTTCn'nnn_ Cuts at: 0 1055 2476 2763 Size: 1055 1421 287 Fragments arranged by size: 1421 1055 287 EcoICRI GAG'CTC Cuts at: 0 939 1584 2763 Size: 939 645 1179 Fragments arranged by size: 1179 939 645 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 138 160 415 499 825 1129 1482 1678 Size: 138 22 255 84 326 304 353 196 Cuts at: 1678 1853 2126 2404 2637 2763 Size: 175 273 278 233 126 Fragments arranged by size: 353 326 304 278 273 255 233 196 175 138 126 84 22 EcoNI CCTnn'n_nnAGG Cuts at: 0 1434 2763 Size: 1434 1329 EcoO109I rG'GnC_Cy Cuts at: 0 288 915 1597 2763 Size: 288 627 682 1166 Fragments arranged by size: 1166 682 627 288 EcoRV GAT'ATC Cuts at: 0 2649 2763 Size: 2649 114 FspI TGC'GCA Cuts at: 0 385 565 2763 Size: 385 180 2198 Fragments arranged by size: 2198 385 180 FspAI rTGC'GCAy Cuts at: 0 565 2763 Size: 565 2198 HaeII r_GCGC'y Cuts at: 0 55 105 157 276 345 2763 Size: 55 50 52 119 69 2418 Fragments arranged by size: 2418 119 69 55 52 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1078 1110 2763 Size: 1078 32 1653 Fragments arranged by size: 1653 1078 32 HincII GTy'rAC Cuts at: 0 129 420 2391 2763 Size: 129 291 1971 372 Fragments arranged by size: 1971 372 291 129 HindIII A'AGCT_T Cuts at: 0 1127 2763 Size: 1127 1636 HpaI GTT'AAC Cuts at: 0 2391 2763 Size: 2391 372 KasI G'GCGC_C Cuts at: 0 51 153 272 2763 Size: 51 102 119 2491 Fragments arranged by size: 2491 119 102 51 MscI TGG'CCA Cuts at: 0 883 955 964 1309 2763 Size: 883 72 9 345 1454 Fragments arranged by size: 1454 883 345 72 9 MslI CAynn'nnrTG Cuts at: 0 250 376 2763 Size: 250 126 2387 Fragments arranged by size: 2387 250 126 MspA1I CmG'CkG Cuts at: 0 165 314 456 2763 Size: 165 149 142 2307 Fragments arranged by size: 2307 165 149 142 NaeI GCC'GGC Cuts at: 0 271 2763 Size: 271 2492 NarI GG'CG_CC Cuts at: 0 52 154 273 2763 Size: 52 102 119 2490 Fragments arranged by size: 2490 119 102 52 NcoI C'CATG_G Cuts at: 0 218 2763 Size: 218 2545 NgoMIV G'CCGG_C Cuts at: 0 269 2763 Size: 269 2494 NotI GC'GGCC_GC Cuts at: 0 781 2763 Size: 781 1982 NspI r_CATG'y Cuts at: 0 753 1387 2004 2214 2751 2763 Size: 753 634 617 210 537 12 Fragments arranged by size: 753 634 617 537 210 12 PasI CC'CwG_GG Cuts at: 0 1290 2763 Size: 1290 1473 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 964 996 2763 Size: 964 32 1767 Fragments arranged by size: 1767 964 32 PpuMI rG'GwC_Cy Cuts at: 0 915 1597 2763 Size: 915 682 1166 Fragments arranged by size: 1166 915 682 PsiI TTA'TAA Cuts at: 0 2566 2763 Size: 2566 197 PspOMI G'GGCC_C Cuts at: 0 288 2763 Size: 288 2475 PspXI vC'TCGA_Gb Cuts at: 0 855 2763 Size: 855 1908 PstI C_TGCA'G Cuts at: 0 455 647 1919 2763 Size: 455 192 1272 844 Fragments arranged by size: 1272 844 455 192 PvuII CAG'CTG Cuts at: 0 456 2763 Size: 456 2307 SacI G_AGCT'C Cuts at: 0 941 1586 2763 Size: 941 645 1177 Fragments arranged by size: 1177 941 645 SacII CC_GC'GG Cuts at: 0 315 2763 Size: 315 2448 SfcI C'TryA_G Cuts at: 0 451 643 1915 2057 2763 Size: 451 192 1272 142 706 Fragments arranged by size: 1272 706 451 192 142 SfiI GGCCn_nnn'nGGCC Cuts at: 0 961 2763 Size: 961 1802 SfoI GGC'GCC Cuts at: 0 53 155 274 2763 Size: 53 102 119 2489 Fragments arranged by size: 2489 119 102 53 SmlI C'TyrA_G Cuts at: 0 499 855 2487 2763 Size: 499 356 1632 276 Fragments arranged by size: 1632 499 356 276 SphI G_CATG'C Cuts at: 0 2751 2763 Size: 2751 12 SspI AAT'ATT Cuts at: 0 2093 2154 2763 Size: 2093 61 609 Fragments arranged by size: 2093 609 61 StuI AGG'CCT Cuts at: 0 1238 1838 2763 Size: 1238 600 925 Fragments arranged by size: 1238 925 600 StyI C'CwwG_G Cuts at: 0 8 218 1600 2025 2763 Size: 8 210 1382 425 738 Fragments arranged by size: 1382 738 425 210 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 502 1021 1528 2763 Size: 502 519 507 1235 Fragments arranged by size: 1235 519 507 502 XhoI C'TCGA_G Cuts at: 0 855 2763 Size: 855 1908 XmnI GAAnn'nnTTC Cuts at: 0 979 1073 2763 Size: 979 94 1690 Fragments arranged by size: 1690 979 94 Enzymes that do cut: AccI AcuI AfeI AflIII AlwNI ApaI ApaLI ApoI AseI AvaI AvrII BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PpiI PpuMI PsiI PspOMI PspXI PstI PvuII SacI SacII SfcI SfiI SfoI SmlI SphI SspI StuI StyI TaqII XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AleI AlfI AloI AscI AsiSI BaeI BamHI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaI BsaBI BsaWI BsgI BsiWI BspEI BspHI BspMI BsrGI BssSI BstZ17I BtgZI ClaI CspCI DraI DraIII EciI EcoRI FalI FseI KpnI MfeI MluI MmeI NdeI NheI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PshAI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TatI TstI Tth111I XbaI XcmI XmaI ZraI