Restriction map for
pF1KE2114
(Linear) (Six-base) MAPSORT of: KIEE2114.seq Check: 6699 from: 1 to: 2646
KIEE2114.orf
With 182 enzymes: SgfI *
September 11, 2017 11:55 ..
AccI GT'mk_AC
Cuts at: 0 186 299 1021 1235 2646
Size: 186 113 722 214 1411
Fragments arranged by size:
1411 722 214 186 113
Acc65I G'GTAC_C
Cuts at: 0 1377 2646
Size: 1377 1269
AclI AA'CG_TT
Cuts at: 0 2252 2646
Size: 2252 394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 2360 2646
Size: 661 1699 286
Fragments arranged by size:
1699 661 286
AflIII A'CryG_T
Cuts at: 0 110 1356 2621 2646
Size: 110 1246 1265 25
Fragments arranged by size:
1265 1246 110 25
AgeI A'CCGG_T
Cuts at: 0 181 2646
Size: 181 2465
AhdI GACnn_n'nnGTC
Cuts at: 0 1267 2646
Size: 1267 1379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2138 2646
Size: 2138 508
AlwNI CAG_nnn'CTG
Cuts at: 0 1096 2646
Size: 1096 1550
ApaI G_GGCC'C
Cuts at: 0 7 2646
Size: 7 2639
ApoI r'AATT_y
Cuts at: 0 165 781 2117 2147 2424 2646
Size: 165 616 1336 30 277 222
Fragments arranged by size:
1336 616 277 222 165 30
AseI AT'TA_AT
Cuts at: 0 1604 2646
Size: 1604 1042
AvaI C'yCGr_G
Cuts at: 0 1631 2243 2646
Size: 1631 612 403
Fragments arranged by size:
1631 612 403
BamHI G'GATC_C
Cuts at: 0 729 2646
Size: 729 1917
BanI G'GyrC_C
Cuts at: 0 120 128 359 1149 1377 1618 2646
Size: 120 8 231 790 228 241 1028
Fragments arranged by size:
1028 790 241 231 228 120 8
BanII G_rGCy'C
Cuts at: 0 7 80 932 2219 2539 2646
Size: 7 73 852 1287 320 107
Fragments arranged by size:
1287 852 320 107 73 7
BbeI G_GCGC'C
Cuts at: 0 132 2646
Size: 132 2514
BbsI GAAGACnn'nnnn_
Cuts at: 0 456 2646
Size: 456 2190
BbvCI CC'TCA_GC
Cuts at: 0 913 2646
Size: 913 1733
BclI T'GATC_A
Cuts at: 0 746 1085 2646
Size: 746 339 1561
Fragments arranged by size:
1561 746 339
BglI GCCn_nnn'nGGC
Cuts at: 0 89 2646
Size: 89 2557
BlpI GC'TnA_GC
Cuts at: 0 1637 2646
Size: 1637 1009
Bme1580I G_kGCm'C
Cuts at: 0 7 125 235 362 2646
Size: 7 118 110 127 2284
Fragments arranged by size:
2284 127 118 110 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 474 1001 2646
Size: 474 527 1645
Fragments arranged by size:
1645 527 474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 1618 1700 2646
Size: 157 1461 82 946
Fragments arranged by size:
1461 946 157 82
Bpu10I CC'TnA_GC
Cuts at: 0 913 926 2646
Size: 913 13 1720
Fragments arranged by size:
1720 913 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1984 2325 2646
Size: 1984 341 321
Fragments arranged by size:
1984 341 321
BsaI GGTCTCn'nnnn_
Cuts at: 0 54 1395 2646
Size: 54 1341 1251
Fragments arranged by size:
1341 1251 54
BsaAI yAC'GTr
Cuts at: 0 1019 1136 1359 1665 1679 2646
Size: 1019 117 223 306 14 967
Fragments arranged by size:
1019 967 306 223 117 14
BsaBI GATnn'nnATC
Cuts at: 0 745 2438 2646
Size: 745 1693 208
Fragments arranged by size:
1693 745 208
BsaHI Gr'CG_yC
Cuts at: 0 98 129 2078 2646
Size: 98 31 1949 568
Fragments arranged by size:
1949 568 98 31
BsaWI w'CCGG_w
Cuts at: 0 181 1608 2646
Size: 181 1427 1038
Fragments arranged by size:
1427 1038 181
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 42 423 2211 2538 2646
Size: 42 381 1788 327 108
Fragments arranged by size:
1788 381 327 108 42
BseYI C'CCAG_C
Cuts at: 0 1218 2646
Size: 1218 1428
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2299 2646
Size: 2299 347
BsiEI CG_ry'CG
Cuts at: 0 186 2646
Size: 186 2460
BsiHKAI G_wGCw'C
Cuts at: 0 825 932 1664 2539 2646
Size: 825 107 732 875 107
Fragments arranged by size:
875 825 732 107 107
BsmI GAATG_Cn'
Cuts at: 0 723 2646
Size: 723 1923
Bsp1286I G_dGCh'C
Cuts at: 0 7 80 125 235 362 825 932 1664
Size: 7 73 45 110 127 463 107 732
Cuts at: 1664 2219 2539 2646
Size: 555 320 107
Fragments arranged by size:
732 555 463 320 127 110 107 107
73 45 7
BspEI T'CCGG_A
Cuts at: 0 1608 2646
Size: 1608 1038
BspHI T'CATG_A
Cuts at: 0 2372 2646
Size: 2372 274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 36 2646
Size: 36 2610
BsrBI CCG'CTC
Cuts at: 0 2203 2646
Size: 2203 443
BsrDI GCAATG_nn'
Cuts at: 0 671 2646
Size: 671 1975
BsrFI r'CCGG_y
Cuts at: 0 181 2646
Size: 181 2465
BssSI C'ACGA_G
Cuts at: 0 2491 2646
Size: 2491 155
BstEII G'GTnAC_C
Cuts at: 0 753 984 2646
Size: 753 231 1662
Fragments arranged by size:
1662 753 231
BstXI CCAn_nnnn'nTGG
Cuts at: 0 996 1596 2646
Size: 996 600 1050
Fragments arranged by size:
1050 996 600
BstYI r'GATC_y
Cuts at: 0 284 729 921 2646
Size: 284 445 192 1725
Fragments arranged by size:
1725 445 284 192
BstZ17I GTA'TAC
Cuts at: 0 1022 1236 2646
Size: 1022 214 1410
Fragments arranged by size:
1410 1022 214
Bsu36I CC'TnA_GG
Cuts at: 0 1826 2646
Size: 1826 820
BtgI C'CryG_G
Cuts at: 0 1421 2646
Size: 1421 1225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 882 2646
Size: 882 1764
BtsI GCAGTG_nn'
Cuts at: 0 1370 1523 1583 2646
Size: 1370 153 60 1063
Fragments arranged by size:
1370 1063 153 60
DraIII CAC_nnn'GTG
Cuts at: 0 702 2646
Size: 702 1944
EaeI y'GGCC_r
Cuts at: 0 579 2646
Size: 579 2067
EarI CTCTTCn'nnn_
Cuts at: 0 451 694 830 2279 2646
Size: 451 243 136 1449 367
Fragments arranged by size:
1449 451 367 243 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2294 2646
Size: 2294 352
EcoICRI GAG'CTC
Cuts at: 0 930 2537 2646
Size: 930 1607 109
Fragments arranged by size:
1607 930 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 661 1618 1700 2360 2646
Size: 157 504 957 82 660 286
Fragments arranged by size:
957 660 504 286 157 82
EcoO109I rG'GnC_Cy
Cuts at: 0 4 2322 2646
Size: 4 2318 324
Fragments arranged by size:
2318 324 4
EcoRI G'AATT_C
Cuts at: 0 781 2147 2646
Size: 781 1366 499
Fragments arranged by size:
1366 781 499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 841 1986 2646
Size: 841 1145 660
Fragments arranged by size:
1145 841 660
HaeII r_GCGC'y
Cuts at: 0 31 132 2646
Size: 31 101 2514
Fragments arranged by size:
2514 101 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 134 166 639 671 1110 1142 1253 1285
Size: 134 32 473 32 439 32 111 32
Cuts at: 1285 2192 2224 2646
Size: 907 32 422
Fragments arranged by size:
907 473 439 422 134 111 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 187 2646
Size: 187 2459
KasI G'GCGC_C
Cuts at: 0 128 2646
Size: 128 2518
KpnI G_GTAC'C
Cuts at: 0 1381 2646
Size: 1381 1265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 734 2646
Size: 563 171 1912
Fragments arranged by size:
1912 563 171
MscI TGG'CCA
Cuts at: 0 581 2646
Size: 581 2065
MslI CAynn'nnrTG
Cuts at: 0 979 1456 1546 2646
Size: 979 477 90 1100
Fragments arranged by size:
1100 979 477 90
MspA1I CmG'CkG
Cuts at: 0 485 1081 2074 2313 2646
Size: 485 596 993 239 333
Fragments arranged by size:
993 596 485 333 239
NarI GG'CG_CC
Cuts at: 0 129 2646
Size: 129 2517
NspI r_CATG'y
Cuts at: 0 2625 2646
Size: 2625 21
PciI A'CATG_T
Cuts at: 0 2621 2646
Size: 2621 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 294 2646
Size: 294 2352
PfoI T'CCnGG_A
Cuts at: 0 828 2646
Size: 828 1818
PmlI CAC'GTG
Cuts at: 0 1665 2646
Size: 1665 981
PspOMI G'GGCC_C
Cuts at: 0 3 2646
Size: 3 2643
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1663 1695 2646
Size: 1663 32 951
Fragments arranged by size:
1663 951 32
PstI C_TGCA'G
Cuts at: 0 47 2646
Size: 47 2599
PvuII CAG'CTG
Cuts at: 0 485 1081 2313 2646
Size: 485 596 1232 333
Fragments arranged by size:
1232 596 485 333
SacI G_AGCT'C
Cuts at: 0 932 2539 2646
Size: 932 1607 107
Fragments arranged by size:
1607 932 107
SalI G'TCGA_C
Cuts at: 0 185 2646
Size: 185 2461
SapI GCTCTTCn'nnn_
Cuts at: 0 830 2646
Size: 830 1816
SexAI A'CCwGG_T
Cuts at: 0 521 2646
Size: 521 2125
SfcI C'TryA_G
Cuts at: 0 43 318 567 1700 2082 2646
Size: 43 275 249 1133 382 564
Fragments arranged by size:
1133 564 382 275 249 43
SfoI GGC'GCC
Cuts at: 0 130 2646
Size: 130 2516
SmaI CCC'GGG
Cuts at: 0 2245 2646
Size: 2245 401
SmlI C'TyrA_G
Cuts at: 0 1999 2304 2646
Size: 1999 305 342
Fragments arranged by size:
1999 342 305
SnaBI TAC'GTA
Cuts at: 0 1019 2646
Size: 1019 1627
StuI AGG'CCT
Cuts at: 0 261 388 2646
Size: 261 127 2258
Fragments arranged by size:
2258 261 127
StyI C'CwwG_G
Cuts at: 0 7 582 2646
Size: 7 575 2064
Fragments arranged by size:
2064 575 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 802 1016 1969 2646
Size: 802 214 953 677
Fragments arranged by size:
953 802 677 214
TatI w'GTAC_w
Cuts at: 0 1346 1922 2646
Size: 1346 576 724
Fragments arranged by size:
1346 724 576
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 824 856 1266 1298 2646
Size: 824 32 410 32 1348
Fragments arranged by size:
1348 824 410 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1419 2646
Size: 1419 1227
XmaI C'CCGG_G
Cuts at: 0 2243 2646
Size: 2243 403
XmnI GAAnn'nnTTC
Cuts at: 0 327 2514 2646
Size: 327 2187 132
Fragments arranged by size:
2187 327 132
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BclI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NarI NspI PciI PflMI PfoI PmlI PspOMI PsrI
PstI PvuII SacI SalI SapI SexAI SfcI SfoI
SmaI SmlI SnaBI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaLI AscI
AsiSI AvrII BaeI BcgI BciVI BfrBI BglII BmgBI
BmtI BplI BsaXI BsiWI BsmBI BsrGI BssHII BstAPI
BstBI ClaI CspCI DraI DrdI EagI EcoNI EcoRV
FseI FspI FspAI HindIII HpaI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PpiI PpuMI PshAI PsiI PspXI PvuI
RsrII SacII SanDI SbfI ScaI SfiI SgfI SgrAI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
ZraI