Restriction map for
pF1KE2119
(Linear) (Six-base) MAPSORT of: KIEE2119.seq Check: 2533 from: 1 to: 2736
KIEE2119.orf
With 182 enzymes: SgfI *
September 29, 2011 13:09 ..
AccI GT'mk_AC
Cuts at: 0 254 2736
Size: 254 2482
Acc65I G'GTAC_C
Cuts at: 0 2233 2736
Size: 2233 503
AclI AA'CG_TT
Cuts at: 0 617 930 2736
Size: 617 313 1806
Fragments arranged by size:
1806 617 313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 892 1421 2711 2736
Size: 325 567 529 1290 25
Fragments arranged by size:
1290 567 529 325 25
AfeI AGC'GCT
Cuts at: 0 2479 2572 2736
Size: 2479 93 164
Fragments arranged by size:
2479 164 93
AflIII A'CryG_T
Cuts at: 0 2294 2736
Size: 2294 442
AhdI GACnn_n'nnGTC
Cuts at: 0 2298 2736
Size: 2298 438
AleI CACnn'nnGTG
Cuts at: 0 1919 2736
Size: 1919 817
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1173 1205 2736
Size: 1173 32 1531
Fragments arranged by size:
1531 1173 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1009 2631 2736
Size: 1009 1622 105
Fragments arranged by size:
1622 1009 105
ApaI G_GGCC'C
Cuts at: 0 98 104 2736
Size: 98 6 2632
Fragments arranged by size:
2632 98 6
ApaLI G'TGCA_C
Cuts at: 0 935 2736
Size: 935 1801
ApoI r'AATT_y
Cuts at: 0 741 1394 1409 1929 2375 2736
Size: 741 653 15 520 446 361
Fragments arranged by size:
741 653 520 446 361 15
AseI AT'TA_AT
Cuts at: 0 977 2736
Size: 977 1759
AvaI C'yCGr_G
Cuts at: 0 97 2736
Size: 97 2639
BanI G'GyrC_C
Cuts at: 0 2084 2233 2736
Size: 2084 149 503
Fragments arranged by size:
2084 503 149
BanII G_rGCy'C
Cuts at: 0 98 104 468 689 1067 1642 2736
Size: 98 6 364 221 378 575 1094
Fragments arranged by size:
1094 575 378 364 221 98 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 425 2366 2736
Size: 425 1941 370
Fragments arranged by size:
1941 425 370
BbvCI CC'TCA_GC
Cuts at: 0 2145 2736
Size: 2145 591
BciVI GTATCCnnnnn_n'
Cuts at: 0 2408 2736
Size: 2408 328
BclI T'GATC_A
Cuts at: 0 480 2651 2736
Size: 480 2171 85
Fragments arranged by size:
2171 480 85
BglI GCCn_nnn'nGGC
Cuts at: 0 67 2333 2736
Size: 67 2266 403
Fragments arranged by size:
2266 403 67
BlpI GC'TnA_GC
Cuts at: 0 991 2736
Size: 991 1745
Bme1580I G_kGCm'C
Cuts at: 0 98 104 678 939 2736
Size: 98 6 574 261 1797
Fragments arranged by size:
1797 574 261 98 6
BmgBI CAC'GTC
Cuts at: 0 228 2736
Size: 228 2508
BmrI ACTGGGnnnn_n'
Cuts at: 0 23 638 1774 2736
Size: 23 615 1136 962
Fragments arranged by size:
1136 962 615 23
BmtI G_CTAG'C
Cuts at: 0 2143 2669 2736
Size: 2143 526 67
Fragments arranged by size:
2143 526 67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 26 2736
Size: 26 2710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 2736
Size: 452 2284
Bpu10I CC'TnA_GC
Cuts at: 0 169 2145 2329 2736
Size: 169 1976 184 407
Fragments arranged by size:
1976 407 184 169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2520 2736
Size: 2520 216
BsaBI GATnn'nnATC
Cuts at: 0 1603 2736
Size: 1603 1133
BsaHI Gr'CG_yC
Cuts at: 0 1172 1951 2736
Size: 1172 779 785
Fragments arranged by size:
1172 785 779
BsaWI w'CCGG_w
Cuts at: 0 12 2216 2736
Size: 12 2204 520
Fragments arranged by size:
2204 520 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1502 1532 1927 1957 2736
Size: 1502 30 395 30 779
Fragments arranged by size:
1502 779 395 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1632 2736
Size: 1632 1104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1249 1595 2736
Size: 1249 346 1141
Fragments arranged by size:
1249 1141 346
BsiEI CG_ry'CG
Cuts at: 0 25 71 137 2736
Size: 25 46 66 2599
Fragments arranged by size:
2599 66 46 25
BsiHKAI G_wGCw'C
Cuts at: 0 468 939 2042 2736
Size: 468 471 1103 694
Fragments arranged by size:
1103 694 471 468
BsmI GAATG_Cn'
Cuts at: 0 2388 2560 2736
Size: 2388 172 176
Fragments arranged by size:
2388 176 172
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1942 2736
Size: 1942 794
Bsp1286I G_dGCh'C
Cuts at: 0 98 104 468 678 689 939 1067 1642
Size: 98 6 364 210 11 250 128 575
Cuts at: 1642 2042 2736
Size: 400 694
Fragments arranged by size:
694 575 400 364 250 210 128 98
11 6
BspEI T'CCGG_A
Cuts at: 0 2216 2736
Size: 2216 520
BspHI T'CATG_A
Cuts at: 0 1217 1268 2427 2736
Size: 1217 51 1159 309
Fragments arranged by size:
1217 1159 309 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1370 2018 2736
Size: 1370 648 718
Fragments arranged by size:
1370 718 648
BsrDI GCAATG_nn'
Cuts at: 0 1094 1118 2736
Size: 1094 24 1618
Fragments arranged by size:
1618 1094 24
BsrFI r'CCGG_y
Cuts at: 0 180 2736
Size: 180 2556
BssSI C'ACGA_G
Cuts at: 0 1165 2071 2736
Size: 1165 906 665
Fragments arranged by size:
1165 906 665
BstAPI GCAn_nnn'nTGC
Cuts at: 0 894 908 2631 2736
Size: 894 14 1723 105
Fragments arranged by size:
1723 894 105 14
BstBI TT'CG_AA
Cuts at: 0 1475 2736
Size: 1475 1261
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2411 2736
Size: 2411 325
BstYI r'GATC_y
Cuts at: 0 299 1149 2736
Size: 299 850 1587
Fragments arranged by size:
1587 850 299
Bsu36I CC'TnA_GG
Cuts at: 0 2162 2255 2263 2599 2736
Size: 2162 93 8 336 137
Fragments arranged by size:
2162 336 137 93 8
BtgI C'CryG_G
Cuts at: 0 44 1646 1986 2736
Size: 44 1602 340 750
Fragments arranged by size:
1602 750 340 44
BtsI GCAGTG_nn'
Cuts at: 0 904 2736
Size: 904 1832
DraI TTT'AAA
Cuts at: 0 570 2097 2736
Size: 570 1527 639
Fragments arranged by size:
1527 639 570
DraIII CAC_nnn'GTG
Cuts at: 0 848 2736
Size: 848 1888
EaeI y'GGCC_r
Cuts at: 0 22 68 356 1583 2736
Size: 22 46 288 1227 1153
Fragments arranged by size:
1227 1153 288 46 22
EagI C'GGCC_G
Cuts at: 0 22 68 2736
Size: 22 46 2668
Fragments arranged by size:
2668 46 22
EarI CTCTTCn'nnn_
Cuts at: 0 727 1243 1812 2736
Size: 727 516 569 924
Fragments arranged by size:
924 727 569 516
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 396 615 2736
Size: 396 219 2121
Fragments arranged by size:
2121 396 219
EcoICRI GAG'CTC
Cuts at: 0 466 2736
Size: 466 2270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 452 892 1421 2711 2736
Size: 325 127 440 529 1290 25
Fragments arranged by size:
1290 529 440 325 127 25
EcoO109I rG'GnC_Cy
Cuts at: 0 2259 2736
Size: 2259 477
EcoRV GAT'ATC
Cuts at: 0 366 1677 2736
Size: 366 1311 1059
Fragments arranged by size:
1311 1059 366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 397 2736
Size: 397 2339
HaeII r_GCGC'y
Cuts at: 0 8 2481 2574 2736
Size: 8 2473 93 162
Fragments arranged by size:
2473 162 93 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 721 753 1134 1166 1271 1303
Size: 310 32 379 32 381 32 105 32
Cuts at: 1303 2284 2316 2736
Size: 981 32 420
Fragments arranged by size:
981 420 381 379 310 105 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 255 2736
Size: 255 2481
HindIII A'AGCT_T
Cuts at: 0 865 2736
Size: 865 1871
KpnI G_GTAC'C
Cuts at: 0 2237 2736
Size: 2237 499
MfeI C'AATT_G
Cuts at: 0 582 2736
Size: 582 2154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1882 2736
Size: 1882 854
MscI TGG'CCA
Cuts at: 0 1585 2736
Size: 1585 1151
MslI CAynn'nnrTG
Cuts at: 0 1267 1919 2588 2736
Size: 1267 652 669 148
Fragments arranged by size:
1267 669 652 148
MspA1I CmG'CkG
Cuts at: 0 36 58 67 345 590 2149 2736
Size: 36 22 9 278 245 1559 587
Fragments arranged by size:
1559 587 278 245 36 22 9
NcoI C'CATG_G
Cuts at: 0 1986 2736
Size: 1986 750
NheI G'CTAG_C
Cuts at: 0 2139 2665 2736
Size: 2139 526 71
Fragments arranged by size:
2139 526 71
NotI GC'GGCC_GC
Cuts at: 0 22 68 2736
Size: 22 46 2668
Fragments arranged by size:
2668 46 22
NspI r_CATG'y
Cuts at: 0 1615 1998 2298 2736
Size: 1615 383 300 438
Fragments arranged by size:
1615 438 383 300
PasI CC'CwG_GG
Cuts at: 0 85 997 2736
Size: 85 912 1739
Fragments arranged by size:
1739 912 85
PciI A'CATG_T
Cuts at: 0 2294 2736
Size: 2294 442
PflMI CCAn_nnn'nTGG
Cuts at: 0 1592 2736
Size: 1592 1144
PfoI T'CCnGG_A
Cuts at: 0 368 2736
Size: 368 2368
PpuMI rG'GwC_Cy
Cuts at: 0 2259 2736
Size: 2259 477
PspOMI G'GGCC_C
Cuts at: 0 94 100 2736
Size: 94 6 2636
Fragments arranged by size:
2636 94 6
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2260 2292 2736
Size: 2260 32 444
Fragments arranged by size:
2260 444 32
PstI C_TGCA'G
Cuts at: 0 161 197 2636 2736
Size: 161 36 2439 100
Fragments arranged by size:
2439 161 100 36
PvuII CAG'CTG
Cuts at: 0 58 345 2149 2736
Size: 58 287 1804 587
Fragments arranged by size:
1804 587 287 58
SacI G_AGCT'C
Cuts at: 0 468 2736
Size: 468 2268
SalI G'TCGA_C
Cuts at: 0 253 2736
Size: 253 2483
ScaI AGT'ACT
Cuts at: 0 856 1763 2736
Size: 856 907 973
Fragments arranged by size:
973 907 856
SexAI A'CCwGG_T
Cuts at: 0 2530 2736
Size: 2530 206
SfcI C'TryA_G
Cuts at: 0 157 193 213 1900 2481 2515 2632 2736
Size: 157 36 20 1687 581 34 117 104
Fragments arranged by size:
1687 581 157 117 104 36 34 20
SmaI CCC'GGG
Cuts at: 0 99 2736
Size: 99 2637
SmlI C'TyrA_G
Cuts at: 0 2499 2736
Size: 2499 237
SphI G_CATG'C
Cuts at: 0 1615 2736
Size: 1615 1121
SrfI GCCC'GGGC
Cuts at: 0 99 2736
Size: 99 2637
StyI C'CwwG_G
Cuts at: 0 960 1634 1986 2509 2736
Size: 960 674 352 523 227
Fragments arranged by size:
960 674 523 352 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2596 2736
Size: 2596 140
TatI w'GTAC_w
Cuts at: 0 854 1761 2736
Size: 854 907 975
Fragments arranged by size:
975 907 854
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1502 1534 2736
Size: 1502 32 1202
Fragments arranged by size:
1502 1202 32
XbaI T'CTAG_A
Cuts at: 0 2289 2736
Size: 2289 447
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1132 2408 2736
Size: 1132 1276 328
Fragments arranged by size:
1276 1132 328
XmaI C'CCGG_G
Cuts at: 0 97 2736
Size: 97 2639
XmnI GAAnn'nnTTC
Cuts at: 0 422 2212 2736
Size: 422 1790 524
Fragments arranged by size:
1790 524 422
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI BanI
BanII BbsI BbvCI BciVI BclI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaBI
BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI DraI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRV FalI HaeII Hin4I HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NcoI NheI NotI NspI
PasI PciI PflMI PfoI PpuMI PspOMI PsrI PstI
PvuII SacI SalI ScaI SexAI SfcI SmaI SmlI
SphI SrfI StyI TaqII TatI TstI XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AlfI AscI AsiSI AvrII
BaeI BamHI BbeI BcgI BfrBI BglII BsaI BsaAI
BseYI BsiWI BsrBI BsrGI BssHII BstEII BstZ17I BtgZI
ClaI CspCI DrdI EcoNI EcoRI FseI FspI FspAI
HpaI KasI MluI NaeI NarI NdeI NgoMIV NruI
NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI
PvuI RsrII SacII SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SnaBI SpeI SspI StuI SwaI Tth111I
XhoI ZraI