(Linear) (Six-base) MAPSORT of: KIEE2256.seq Check: 1637 from: 1 to: 1257 KIEE2256.orf With 182 enzymes: SgfI * July 20, 2018 17:51 .. AatII G_ACGT'C Cuts at: 0 347 1257 Size: 347 910 AccI GT'mk_AC Cuts at: 0 275 581 1257 Size: 275 306 676 Fragments arranged by size: 676 306 275 AfeI AGC'GCT Cuts at: 0 661 1257 Size: 661 596 AlwNI CAG_nnn'CTG Cuts at: 0 459 1110 1257 Size: 459 651 147 Fragments arranged by size: 651 459 147 ApaLI G'TGCA_C Cuts at: 0 354 1093 1257 Size: 354 739 164 Fragments arranged by size: 739 354 164 AvaI C'yCGr_G Cuts at: 0 809 1257 Size: 809 448 BanI G'GyrC_C Cuts at: 0 469 501 1257 Size: 469 32 756 Fragments arranged by size: 756 469 32 BanII G_rGCy'C Cuts at: 0 313 1257 Size: 313 944 BbeI G_GCGC'C Cuts at: 0 473 505 1257 Size: 473 32 752 Fragments arranged by size: 752 473 32 BbsI GAAGACnn'nnnn_ Cuts at: 0 1197 1221 1257 Size: 1197 24 36 Fragments arranged by size: 1197 36 24 BglI GCCn_nnn'nGGC Cuts at: 0 61 334 1137 1257 Size: 61 273 803 120 Fragments arranged by size: 803 273 120 61 BlpI GC'TnA_GC Cuts at: 0 307 1257 Size: 307 950 Bme1580I G_kGCm'C Cuts at: 0 358 1097 1133 1257 Size: 358 739 36 124 Fragments arranged by size: 739 358 124 36 BmgBI CAC'GTC Cuts at: 0 528 676 1257 Size: 528 148 581 Fragments arranged by size: 581 528 148 BmrI ACTGGGnnnn_n' Cuts at: 0 645 1257 Size: 645 612 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 580 1257 Size: 580 677 BsaAI yAC'GTr Cuts at: 0 476 576 1257 Size: 476 100 681 Fragments arranged by size: 681 476 100 BsaHI Gr'CG_yC Cuts at: 0 199 326 344 470 502 941 1078 1257 Size: 199 127 18 126 32 439 137 179 Fragments arranged by size: 439 199 179 137 127 126 32 18 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 984 1014 1257 Size: 984 30 243 Fragments arranged by size: 984 243 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 232 235 1257 Size: 232 3 1022 Fragments arranged by size: 1022 232 3 BseYI C'CCAG_C Cuts at: 0 40 91 662 712 847 1161 1257 Size: 40 51 571 50 135 314 96 Fragments arranged by size: 571 314 135 96 51 50 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 673 1257 Size: 673 584 BsiEI CG_ry'CG Cuts at: 0 56 233 537 696 1017 1257 Size: 56 177 304 159 321 240 Fragments arranged by size: 321 304 240 177 159 56 BsiHKAI G_wGCw'C Cuts at: 0 146 358 598 933 1097 1257 Size: 146 212 240 335 164 160 Fragments arranged by size: 335 240 212 164 160 146 Bsp1286I G_dGCh'C Cuts at: 0 146 313 358 598 933 1097 1133 1257 Size: 146 167 45 240 335 164 36 124 Fragments arranged by size: 335 240 167 164 146 124 45 36 BsrBI CCG'CTC Cuts at: 0 267 618 1257 Size: 267 351 639 Fragments arranged by size: 639 351 267 BsrFI r'CCGG_y Cuts at: 0 51 431 449 1012 1044 1257 Size: 51 380 18 563 32 213 Fragments arranged by size: 563 380 213 51 32 18 BssHII G'CGCG_C Cuts at: 0 186 698 1257 Size: 186 512 559 Fragments arranged by size: 559 512 186 BssSI C'ACGA_G Cuts at: 0 160 1257 Size: 160 1097 BstAPI GCAn_nnn'nTGC Cuts at: 0 459 1257 Size: 459 798 BstXI CCAn_nnnn'nTGG Cuts at: 0 390 1257 Size: 390 867 BtgI C'CryG_G Cuts at: 0 17 56 338 407 488 648 1135 1257 Size: 17 39 282 69 81 160 487 122 Fragments arranged by size: 487 282 160 122 81 69 39 17 DrdI GACnn_nn'nnGTC Cuts at: 0 577 1257 Size: 577 680 EaeI y'GGCC_r Cuts at: 0 53 176 230 335 404 410 534 693 Size: 53 123 54 105 69 6 124 159 Cuts at: 693 1014 1257 Size: 321 243 Fragments arranged by size: 321 243 159 124 123 105 69 54 53 6 EagI C'GGCC_G Cuts at: 0 53 230 534 693 1014 1257 Size: 53 177 304 159 321 243 Fragments arranged by size: 321 304 243 177 159 53 EarI CTCTTCn'nnn_ Cuts at: 0 274 1257 Size: 274 983 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 580 1257 Size: 580 677 EcoNI CCTnn'n_nnAGG Cuts at: 0 760 856 1257 Size: 760 96 401 Fragments arranged by size: 760 401 96 EcoO109I rG'GnC_Cy Cuts at: 0 765 814 861 1038 1058 1239 1257 Size: 765 49 47 177 20 181 18 Fragments arranged by size: 765 181 177 49 47 20 18 FseI GG_CCGG'CC Cuts at: 0 55 1257 Size: 55 1202 HaeII r_GCGC'y Cuts at: 0 112 473 505 663 1111 1151 1181 1257 Size: 112 361 32 158 448 40 30 76 Fragments arranged by size: 448 361 158 112 76 40 32 30 HincII GTy'rAC Cuts at: 0 1173 1257 Size: 1173 84 KasI G'GCGC_C Cuts at: 0 469 501 1257 Size: 469 32 756 Fragments arranged by size: 756 469 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 947 1257 Size: 947 310 MscI TGG'CCA Cuts at: 0 178 337 1257 Size: 178 159 920 Fragments arranged by size: 920 178 159 MslI CAynn'nnrTG Cuts at: 0 337 388 1257 Size: 337 51 869 Fragments arranged by size: 869 337 51 MspA1I CmG'CkG Cuts at: 0 19 58 420 456 490 650 716 1137 Size: 19 39 362 36 34 160 66 421 Cuts at: 1137 1257 Size: 120 Fragments arranged by size: 421 362 160 120 66 39 36 34 19 NaeI GCC'GGC Cuts at: 0 53 451 1257 Size: 53 398 806 Fragments arranged by size: 806 398 53 NarI GG'CG_CC Cuts at: 0 470 502 1257 Size: 470 32 755 Fragments arranged by size: 755 470 32 NcoI C'CATG_G Cuts at: 0 338 1257 Size: 338 919 NgoMIV G'CCGG_C Cuts at: 0 51 449 1257 Size: 51 398 808 Fragments arranged by size: 808 398 51 NotI GC'GGCC_GC Cuts at: 0 230 534 1257 Size: 230 304 723 Fragments arranged by size: 723 304 230 PmlI CAC'GTG Cuts at: 0 476 1257 Size: 476 781 PpuMI rG'GwC_Cy Cuts at: 0 765 814 861 1038 1257 Size: 765 49 47 177 219 Fragments arranged by size: 765 219 177 49 47 PstI C_TGCA'G Cuts at: 0 197 659 1257 Size: 197 462 598 Fragments arranged by size: 598 462 197 PvuII CAG'CTG Cuts at: 0 456 1257 Size: 456 801 SacII CC_GC'GG Cuts at: 0 20 59 491 651 1138 1257 Size: 20 39 432 160 487 119 Fragments arranged by size: 487 432 160 119 39 20 SanDI GG'GwC_CC Cuts at: 0 814 1257 Size: 814 443 SapI GCTCTTCn'nnn_ Cuts at: 0 274 1257 Size: 274 983 SfcI C'TryA_G Cuts at: 0 193 655 1257 Size: 193 462 602 Fragments arranged by size: 602 462 193 SfoI GGC'GCC Cuts at: 0 471 503 1257 Size: 471 32 754 Fragments arranged by size: 754 471 32 SmaI CCC'GGG Cuts at: 0 811 1257 Size: 811 446 StyI C'CwwG_G Cuts at: 0 338 1257 Size: 338 919 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 984 1016 1257 Size: 984 32 241 Fragments arranged by size: 984 241 32 Tth111I GACn'n_nGTC Cuts at: 0 943 1257 Size: 943 314 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 865 1257 Size: 865 392 XmaI C'CCGG_G Cuts at: 0 809 1257 Size: 809 448 ZraI GAC'GTC Cuts at: 0 345 1257 Size: 345 912 Enzymes that do cut: AatII AccI AfeI AlwNI ApaLI AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BmrI BpmI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssHII BssSI BstAPI BstXI BtgI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I FseI HaeII HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI PmlI PpuMI PstI PvuII SacII SanDI SapI SfcI SfoI SmaI StyI TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EciI EcoICRI EcoRI EcoRV FalI FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI NdeI NheI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI XbaI XhoI XmnI