(Linear) (Six-base) MAPSORT of: KIEE2259.seq Check: 9668 from: 1 to: 1506 KIEE2259.orf With 182 enzymes: SgfI * October 2, 2018 18:08 .. AccI GT'mk_AC Cuts at: 0 158 1506 Size: 158 1348 Acc65I G'GTAC_C Cuts at: 0 867 1506 Size: 867 639 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 517 1506 Size: 517 989 AfeI AGC'GCT Cuts at: 0 361 1506 Size: 361 1145 AflIII A'CryG_T Cuts at: 0 53 386 1506 Size: 53 333 1120 Fragments arranged by size: 1120 333 53 AleI CACnn'nnGTG Cuts at: 0 385 1506 Size: 385 1121 AlwNI CAG_nnn'CTG Cuts at: 0 420 1506 Size: 420 1086 ApaLI G'TGCA_C Cuts at: 0 1183 1506 Size: 1183 323 ApoI r'AATT_y Cuts at: 0 396 1506 Size: 396 1110 AvaI C'yCGr_G Cuts at: 0 1042 1279 1506 Size: 1042 237 227 Fragments arranged by size: 1042 237 227 BanI G'GyrC_C Cuts at: 0 867 1122 1506 Size: 867 255 384 Fragments arranged by size: 867 384 255 BanII G_rGCy'C Cuts at: 0 647 1506 Size: 647 859 BbeI G_GCGC'C Cuts at: 0 1126 1506 Size: 1126 380 BbsI GAAGACnn'nnnn_ Cuts at: 0 298 813 1422 1506 Size: 298 515 609 84 Fragments arranged by size: 609 515 298 84 BclI T'GATC_A Cuts at: 0 902 1506 Size: 902 604 BglI GCCn_nnn'nGGC Cuts at: 0 1178 1506 Size: 1178 328 Bme1580I G_kGCm'C Cuts at: 0 1187 1506 Size: 1187 319 BmgBI CAC'GTC Cuts at: 0 192 1506 Size: 192 1314 BmrI ACTGGGnnnn_n' Cuts at: 0 876 1506 Size: 876 630 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 410 1330 1506 Size: 410 920 176 Fragments arranged by size: 920 410 176 Bpu10I CC'TnA_GC Cuts at: 0 169 1506 Size: 169 1337 BsaAI yAC'GTr Cuts at: 0 54 1506 Size: 54 1452 BsaHI Gr'CG_yC Cuts at: 0 20 368 1123 1506 Size: 20 348 755 383 Fragments arranged by size: 755 383 348 20 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1474 1504 1506 Size: 1474 30 2 Fragments arranged by size: 1474 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 627 1327 1506 Size: 627 700 179 Fragments arranged by size: 700 627 179 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 502 1246 1428 1506 Size: 34 468 744 182 78 Fragments arranged by size: 744 468 182 78 34 BsiHKAI G_wGCw'C Cuts at: 0 728 1187 1256 1506 Size: 728 459 69 250 Fragments arranged by size: 728 459 250 69 Bsp1286I G_dGCh'C Cuts at: 0 647 728 1187 1256 1506 Size: 647 81 459 69 250 Fragments arranged by size: 647 459 250 81 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 428 716 1157 1506 Size: 428 288 441 349 Fragments arranged by size: 441 428 349 288 BsrDI GCAATG_nn' Cuts at: 0 408 1325 1506 Size: 408 917 181 Fragments arranged by size: 917 408 181 BsrGI T'GTAC_A Cuts at: 0 1154 1506 Size: 1154 352 BssSI C'ACGA_G Cuts at: 0 832 1506 Size: 832 674 BstAPI GCAn_nnn'nTGC Cuts at: 0 1232 1260 1506 Size: 1232 28 246 Fragments arranged by size: 1232 246 28 BstXI CCAn_nnnn'nTGG Cuts at: 0 1308 1479 1506 Size: 1308 171 27 Fragments arranged by size: 1308 171 27 BstYI r'GATC_y Cuts at: 0 534 1304 1506 Size: 534 770 202 Fragments arranged by size: 770 534 202 BtgI C'CryG_G Cuts at: 0 128 1118 1167 1506 Size: 128 990 49 339 Fragments arranged by size: 990 339 128 49 BtsI GCAGTG_nn' Cuts at: 0 486 1506 Size: 486 1020 ClaI AT'CG_AT Cuts at: 0 455 1506 Size: 455 1051 EaeI y'GGCC_r Cuts at: 0 35 131 312 1097 1221 1298 1506 Size: 35 96 181 785 124 77 208 Fragments arranged by size: 785 208 181 124 96 77 35 EarI CTCTTCn'nnn_ Cuts at: 0 1030 1506 Size: 1030 476 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 723 1506 Size: 723 783 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 410 517 1330 1506 Size: 410 107 813 176 Fragments arranged by size: 813 410 176 107 EcoNI CCTnn'n_nnAGG Cuts at: 0 120 1312 1506 Size: 120 1192 194 Fragments arranged by size: 1192 194 120 EcoO109I rG'GnC_Cy Cuts at: 0 506 588 1287 1506 Size: 506 82 699 219 Fragments arranged by size: 699 506 219 82 EcoRI G'AATT_C Cuts at: 0 396 1506 Size: 396 1110 EcoRV GAT'ATC Cuts at: 0 558 1506 Size: 558 948 HaeII r_GCGC'y Cuts at: 0 34 363 1089 1118 1126 1506 Size: 34 329 726 29 8 380 Fragments arranged by size: 726 380 329 34 29 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1452 1474 1484 1506 Size: 1452 22 10 22 Fragments arranged by size: 1452 22 22 10 HindIII A'AGCT_T Cuts at: 0 82 1506 Size: 82 1424 KasI G'GCGC_C Cuts at: 0 1122 1506 Size: 1122 384 KpnI G_GTAC'C Cuts at: 0 871 1506 Size: 871 635 MscI TGG'CCA Cuts at: 0 133 314 1099 1300 1506 Size: 133 181 785 201 206 Fragments arranged by size: 785 206 201 181 133 MslI CAynn'nnrTG Cuts at: 0 190 385 1459 1506 Size: 190 195 1074 47 Fragments arranged by size: 1074 195 190 47 MspA1I CmG'CkG Cuts at: 0 1083 1169 1343 1506 Size: 1083 86 174 163 Fragments arranged by size: 1083 174 163 86 NarI GG'CG_CC Cuts at: 0 1123 1506 Size: 1123 383 PasI CC'CwG_GG Cuts at: 0 789 1506 Size: 789 717 PflMI CCAn_nnn'nTGG Cuts at: 0 227 1506 Size: 227 1279 PfoI T'CCnGG_A Cuts at: 0 1307 1506 Size: 1307 199 PmlI CAC'GTG Cuts at: 0 54 1506 Size: 54 1452 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1366 1398 1506 Size: 1366 32 108 Fragments arranged by size: 1366 108 32 PpuMI rG'GwC_Cy Cuts at: 0 506 588 1287 1506 Size: 506 82 699 219 Fragments arranged by size: 699 506 219 82 PshAI GACnn'nnGTC Cuts at: 0 994 1506 Size: 994 512 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1138 1170 1506 Size: 1138 32 336 Fragments arranged by size: 1138 336 32 PstI C_TGCA'G Cuts at: 0 182 542 768 947 1093 1372 1506 Size: 182 360 226 179 146 279 134 Fragments arranged by size: 360 279 226 182 179 146 134 SacII CC_GC'GG Cuts at: 0 1170 1506 Size: 1170 336 SanDI GG'GwC_CC Cuts at: 0 1287 1506 Size: 1287 219 ScaI AGT'ACT Cuts at: 0 886 1506 Size: 886 620 SfcI C'TryA_G Cuts at: 0 178 538 764 943 1089 1368 1506 Size: 178 360 226 179 146 279 138 Fragments arranged by size: 360 279 226 179 178 146 138 SfoI GGC'GCC Cuts at: 0 1124 1506 Size: 1124 382 SmaI CCC'GGG Cuts at: 0 1044 1506 Size: 1044 462 StyI C'CwwG_G Cuts at: 0 116 530 1506 Size: 116 414 976 Fragments arranged by size: 976 414 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1416 1506 Size: 1416 90 TatI w'GTAC_w Cuts at: 0 884 1154 1506 Size: 884 270 352 Fragments arranged by size: 884 352 270 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1393 1425 1506 Size: 1393 32 81 Fragments arranged by size: 1393 81 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 906 1506 Size: 906 600 XmaI C'CCGG_G Cuts at: 0 1042 1506 Size: 1042 464 Enzymes that do cut: AccI Acc65I AcuI AfeI AflIII AleI AlwNI ApaLI ApoI AvaI BanI BanII BbeI BbsI BclI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrGI BssSI BstAPI BstXI BstYI BtgI BtsI ClaI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HindIII KasI KpnI MscI MslI MspA1I NarI PasI PflMI PfoI PmlI PpiI PpuMI PshAI PsrI PstI SacII SanDI ScaI SfcI SfoI SmaI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AclI AflII AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglII BlpI BmtI BplI BpuEI BsaI BsaBI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EcoICRI FalI FseI FspI FspAI HincII HpaI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PsiI PspOMI PspXI PvuI PvuII RsrII SacI SalI SapI SbfI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI