Restriction map for
pF1KE2259
(Linear) (Six-base) MAPSORT of: KIEE2259.seq Check: 9668 from: 1 to: 1506
KIEE2259.orf
With 182 enzymes: SgfI *
October 2, 2018 18:08 ..
AccI GT'mk_AC
Cuts at: 0 158 1506
Size: 158 1348
Acc65I G'GTAC_C
Cuts at: 0 867 1506
Size: 867 639
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1506
Size: 517 989
AfeI AGC'GCT
Cuts at: 0 361 1506
Size: 361 1145
AflIII A'CryG_T
Cuts at: 0 53 386 1506
Size: 53 333 1120
Fragments arranged by size:
1120 333 53
AleI CACnn'nnGTG
Cuts at: 0 385 1506
Size: 385 1121
AlwNI CAG_nnn'CTG
Cuts at: 0 420 1506
Size: 420 1086
ApaLI G'TGCA_C
Cuts at: 0 1183 1506
Size: 1183 323
ApoI r'AATT_y
Cuts at: 0 396 1506
Size: 396 1110
AvaI C'yCGr_G
Cuts at: 0 1042 1279 1506
Size: 1042 237 227
Fragments arranged by size:
1042 237 227
BanI G'GyrC_C
Cuts at: 0 867 1122 1506
Size: 867 255 384
Fragments arranged by size:
867 384 255
BanII G_rGCy'C
Cuts at: 0 647 1506
Size: 647 859
BbeI G_GCGC'C
Cuts at: 0 1126 1506
Size: 1126 380
BbsI GAAGACnn'nnnn_
Cuts at: 0 298 813 1422 1506
Size: 298 515 609 84
Fragments arranged by size:
609 515 298 84
BclI T'GATC_A
Cuts at: 0 902 1506
Size: 902 604
BglI GCCn_nnn'nGGC
Cuts at: 0 1178 1506
Size: 1178 328
Bme1580I G_kGCm'C
Cuts at: 0 1187 1506
Size: 1187 319
BmgBI CAC'GTC
Cuts at: 0 192 1506
Size: 192 1314
BmrI ACTGGGnnnn_n'
Cuts at: 0 876 1506
Size: 876 630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 1330 1506
Size: 410 920 176
Fragments arranged by size:
920 410 176
Bpu10I CC'TnA_GC
Cuts at: 0 169 1506
Size: 169 1337
BsaAI yAC'GTr
Cuts at: 0 54 1506
Size: 54 1452
BsaHI Gr'CG_yC
Cuts at: 0 20 368 1123 1506
Size: 20 348 755 383
Fragments arranged by size:
755 383 348 20
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1474 1504 1506
Size: 1474 30 2
Fragments arranged by size:
1474 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 627 1327 1506
Size: 627 700 179
Fragments arranged by size:
700 627 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 502 1246 1428 1506
Size: 34 468 744 182 78
Fragments arranged by size:
744 468 182 78 34
BsiHKAI G_wGCw'C
Cuts at: 0 728 1187 1256 1506
Size: 728 459 69 250
Fragments arranged by size:
728 459 250 69
Bsp1286I G_dGCh'C
Cuts at: 0 647 728 1187 1256 1506
Size: 647 81 459 69 250
Fragments arranged by size:
647 459 250 81 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 428 716 1157 1506
Size: 428 288 441 349
Fragments arranged by size:
441 428 349 288
BsrDI GCAATG_nn'
Cuts at: 0 408 1325 1506
Size: 408 917 181
Fragments arranged by size:
917 408 181
BsrGI T'GTAC_A
Cuts at: 0 1154 1506
Size: 1154 352
BssSI C'ACGA_G
Cuts at: 0 832 1506
Size: 832 674
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1232 1260 1506
Size: 1232 28 246
Fragments arranged by size:
1232 246 28
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1308 1479 1506
Size: 1308 171 27
Fragments arranged by size:
1308 171 27
BstYI r'GATC_y
Cuts at: 0 534 1304 1506
Size: 534 770 202
Fragments arranged by size:
770 534 202
BtgI C'CryG_G
Cuts at: 0 128 1118 1167 1506
Size: 128 990 49 339
Fragments arranged by size:
990 339 128 49
BtsI GCAGTG_nn'
Cuts at: 0 486 1506
Size: 486 1020
ClaI AT'CG_AT
Cuts at: 0 455 1506
Size: 455 1051
EaeI y'GGCC_r
Cuts at: 0 35 131 312 1097 1221 1298 1506
Size: 35 96 181 785 124 77 208
Fragments arranged by size:
785 208 181 124 96 77 35
EarI CTCTTCn'nnn_
Cuts at: 0 1030 1506
Size: 1030 476
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 723 1506
Size: 723 783
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 517 1330 1506
Size: 410 107 813 176
Fragments arranged by size:
813 410 176 107
EcoNI CCTnn'n_nnAGG
Cuts at: 0 120 1312 1506
Size: 120 1192 194
Fragments arranged by size:
1192 194 120
EcoO109I rG'GnC_Cy
Cuts at: 0 506 588 1287 1506
Size: 506 82 699 219
Fragments arranged by size:
699 506 219 82
EcoRI G'AATT_C
Cuts at: 0 396 1506
Size: 396 1110
EcoRV GAT'ATC
Cuts at: 0 558 1506
Size: 558 948
HaeII r_GCGC'y
Cuts at: 0 34 363 1089 1118 1126 1506
Size: 34 329 726 29 8 380
Fragments arranged by size:
726 380 329 34 29 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1452 1474 1484 1506
Size: 1452 22 10 22
Fragments arranged by size:
1452 22 22 10
HindIII A'AGCT_T
Cuts at: 0 82 1506
Size: 82 1424
KasI G'GCGC_C
Cuts at: 0 1122 1506
Size: 1122 384
KpnI G_GTAC'C
Cuts at: 0 871 1506
Size: 871 635
MscI TGG'CCA
Cuts at: 0 133 314 1099 1300 1506
Size: 133 181 785 201 206
Fragments arranged by size:
785 206 201 181 133
MslI CAynn'nnrTG
Cuts at: 0 190 385 1459 1506
Size: 190 195 1074 47
Fragments arranged by size:
1074 195 190 47
MspA1I CmG'CkG
Cuts at: 0 1083 1169 1343 1506
Size: 1083 86 174 163
Fragments arranged by size:
1083 174 163 86
NarI GG'CG_CC
Cuts at: 0 1123 1506
Size: 1123 383
PasI CC'CwG_GG
Cuts at: 0 789 1506
Size: 789 717
PflMI CCAn_nnn'nTGG
Cuts at: 0 227 1506
Size: 227 1279
PfoI T'CCnGG_A
Cuts at: 0 1307 1506
Size: 1307 199
PmlI CAC'GTG
Cuts at: 0 54 1506
Size: 54 1452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1366 1398 1506
Size: 1366 32 108
Fragments arranged by size:
1366 108 32
PpuMI rG'GwC_Cy
Cuts at: 0 506 588 1287 1506
Size: 506 82 699 219
Fragments arranged by size:
699 506 219 82
PshAI GACnn'nnGTC
Cuts at: 0 994 1506
Size: 994 512
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1138 1170 1506
Size: 1138 32 336
Fragments arranged by size:
1138 336 32
PstI C_TGCA'G
Cuts at: 0 182 542 768 947 1093 1372 1506
Size: 182 360 226 179 146 279 134
Fragments arranged by size:
360 279 226 182 179 146 134
SacII CC_GC'GG
Cuts at: 0 1170 1506
Size: 1170 336
SanDI GG'GwC_CC
Cuts at: 0 1287 1506
Size: 1287 219
ScaI AGT'ACT
Cuts at: 0 886 1506
Size: 886 620
SfcI C'TryA_G
Cuts at: 0 178 538 764 943 1089 1368 1506
Size: 178 360 226 179 146 279 138
Fragments arranged by size:
360 279 226 179 178 146 138
SfoI GGC'GCC
Cuts at: 0 1124 1506
Size: 1124 382
SmaI CCC'GGG
Cuts at: 0 1044 1506
Size: 1044 462
StyI C'CwwG_G
Cuts at: 0 116 530 1506
Size: 116 414 976
Fragments arranged by size:
976 414 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1416 1506
Size: 1416 90
TatI w'GTAC_w
Cuts at: 0 884 1154 1506
Size: 884 270 352
Fragments arranged by size:
884 352 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1393 1425 1506
Size: 1393 32 81
Fragments arranged by size:
1393 81 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 906 1506
Size: 906 600
XmaI C'CCGG_G
Cuts at: 0 1042 1506
Size: 1042 464
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AleI AlwNI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BclI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaHI BsaXI
BseRI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrGI BssSI
BstAPI BstXI BstYI BtgI BtsI ClaI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I
HindIII KasI KpnI MscI MslI MspA1I NarI PasI
PflMI PfoI PmlI PpiI PpuMI PshAI PsrI PstI
SacII SanDI ScaI SfcI SfoI SmaI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AlfI AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BfrBI BglII BlpI BmtI BplI BpuEI
BsaI BsaBI BsaWI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BssHII BstBI BstEII BstZ17I
Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EcoICRI
FalI FseI FspI FspAI HincII HpaI MfeI MluI
MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PsiI PspOMI PspXI
PvuI PvuII RsrII SacI SalI SapI SbfI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI