Restriction map for
pF1KE2267
(Linear) (Six-base) MAPSORT of: KIEE2267.seq Check: 5217 from: 1 to: 2844
KIEE2267.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 0 1709 2844
Size: 1709 1135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1495 2006 2844
Size: 529 966 511 838
Fragments arranged by size:
966 838 529 511
AflIII A'CryG_T
Cuts at: 0 726 728 2216 2761 2844
Size: 726 2 1488 545 83
Fragments arranged by size:
1488 726 545 83 2
AleI CACnn'nnGTG
Cuts at: 0 2750 2844
Size: 2750 94
AlwNI CAG_nnn'CTG
Cuts at: 0 1993 2755 2844
Size: 1993 762 89
Fragments arranged by size:
1993 762 89
ApaI G_GGCC'C
Cuts at: 0 1188 1671 2844
Size: 1188 483 1173
Fragments arranged by size:
1188 1173 483
ApoI r'AATT_y
Cuts at: 0 1435 1768 2661 2844
Size: 1435 333 893 183
Fragments arranged by size:
1435 893 333 183
AvaI C'yCGr_G
Cuts at: 0 132 511 1275 1549 2844
Size: 132 379 764 274 1295
Fragments arranged by size:
1295 764 379 274 132
BamHI G'GATC_C
Cuts at: 0 115 1728 2844
Size: 115 1613 1116
Fragments arranged by size:
1613 1116 115
BanI G'GyrC_C
Cuts at: 0 2335 2434 2844
Size: 2335 99 410
Fragments arranged by size:
2335 410 99
BanII G_rGCy'C
Cuts at: 0 133 1188 1671 2636 2844
Size: 133 1055 483 965 208
Fragments arranged by size:
1055 965 483 208 133
BbsI GAAGACnn'nnnn_
Cuts at: 0 1907 2211 2816 2844
Size: 1907 304 605 28
Fragments arranged by size:
1907 605 304 28
BbvCI CC'TCA_GC
Cuts at: 0 2554 2844
Size: 2554 290
BfrBI ATG'CAT
Cuts at: 0 1579 2844
Size: 1579 1265
BglII A'GATC_T
Cuts at: 0 2249 2844
Size: 2249 595
Bme1580I G_kGCm'C
Cuts at: 0 1188 1671 2844
Size: 1188 483 1173
Fragments arranged by size:
1188 1173 483
BmrI ACTGGGnnnn_n'
Cuts at: 0 1080 2844
Size: 1080 1764
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 949 2844
Size: 949 1895
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1225 1546 1836 2844
Size: 1225 321 290 1008
Fragments arranged by size:
1225 1008 321 290
Bpu10I CC'TnA_GC
Cuts at: 0 2554 2844
Size: 2554 290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 2844
Size: 902 1942
BsaI GGTCTCn'nnnn_
Cuts at: 0 2010 2844
Size: 2010 834
BsaAI yAC'GTr
Cuts at: 0 729 2844
Size: 729 2115
BsaHI Gr'CG_yC
Cuts at: 0 703 2208 2844
Size: 703 1505 636
Fragments arranged by size:
1505 703 636
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1066 1096 2844
Size: 1066 30 1748
Fragments arranged by size:
1748 1066 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 2604 2844
Size: 193 2411 240
Fragments arranged by size:
2411 240 193
BseYI C'CCAG_C
Cuts at: 0 305 2844
Size: 305 2539
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 658 2614 2844
Size: 78 580 1956 230
Fragments arranged by size:
1956 580 230 78
BsiEI CG_ry'CG
Cuts at: 0 73 1065 2844
Size: 73 992 1779
Fragments arranged by size:
1779 992 73
BsiHKAI G_wGCw'C
Cuts at: 0 965 980 1967 2844
Size: 965 15 987 877
Fragments arranged by size:
987 965 877 15
BsmI GAATG_Cn'
Cuts at: 0 688 2609 2844
Size: 688 1921 235
Fragments arranged by size:
1921 688 235
BsmBI CGTCTCn'nnnn_
Cuts at: 0 529 2844
Size: 529 2315
Bsp1286I G_dGCh'C
Cuts at: 0 133 965 980 1188 1671 1967 2636 2844
Size: 133 832 15 208 483 296 669 208
Fragments arranged by size:
832 669 483 296 208 208 133 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 50 2844
Size: 50 2794
BsrDI GCAATG_nn'
Cuts at: 0 944 2019 2844
Size: 944 1075 825
Fragments arranged by size:
1075 944 825
BssSI C'ACGA_G
Cuts at: 0 1612 1960 2844
Size: 1612 348 884
Fragments arranged by size:
1612 884 348
BstEII G'GTnAC_C
Cuts at: 0 580 2844
Size: 580 2264
BstYI r'GATC_y
Cuts at: 0 115 1164 1728 2017 2249 2844
Size: 115 1049 564 289 232 595
Fragments arranged by size:
1049 595 564 289 232 115
BtgI C'CryG_G
Cuts at: 0 24 73 1102 2844
Size: 24 49 1029 1742
Fragments arranged by size:
1742 1029 49 24
BtsI GCAGTG_nn'
Cuts at: 0 61 980 1009 2522 2844
Size: 61 919 29 1513 322
Fragments arranged by size:
1513 919 322 61 29
DraI TTT'AAA
Cuts at: 0 1419 1833 2405 2844
Size: 1419 414 572 439
Fragments arranged by size:
1419 572 439 414
DraIII CAC_nnn'GTG
Cuts at: 0 969 2844
Size: 969 1875
EaeI y'GGCC_r
Cuts at: 0 70 1058 2363 2474 2844
Size: 70 988 1305 111 370
Fragments arranged by size:
1305 988 370 111 70
EagI C'GGCC_G
Cuts at: 0 70 2844
Size: 70 2774
EarI CTCTTCn'nnn_
Cuts at: 0 1275 1561 1883 2844
Size: 1275 286 322 961
Fragments arranged by size:
1275 961 322 286
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 802 988 2844
Size: 802 186 1856
Fragments arranged by size:
1856 802 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1225 1495 1546 1836 2006 2844
Size: 529 696 270 51 290 170 838
Fragments arranged by size:
838 696 529 290 270 170 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1192 2844
Size: 1192 1652
EcoO109I rG'GnC_Cy
Cuts at: 0 62 1184 1185 1667 2463 2706 2844
Size: 62 1122 1 482 796 243 138
Fragments arranged by size:
1122 796 482 243 138 62 1
EcoRI G'AATT_C
Cuts at: 0 1435 2844
Size: 1435 1409
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 286 318 1791 1823 2708 2740 2844
Size: 286 32 1473 32 885 32 104
Fragments arranged by size:
1473 885 286 104 32 32 32
HincII GTy'rAC
Cuts at: 0 1515 2844
Size: 1515 1329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1049 1460 2844
Size: 914 135 411 1384
Fragments arranged by size:
1384 914 411 135
MslI CAynn'nnrTG
Cuts at: 0 2221 2234 2482 2590 2750 2766 2844
Size: 2221 13 248 108 160 16 78
Fragments arranged by size:
2221 248 160 108 78 16 13
MspA1I CmG'CkG
Cuts at: 0 26 75 1070 2844
Size: 26 49 995 1774
Fragments arranged by size:
1774 995 49 26
NcoI C'CATG_G
Cuts at: 0 1102 2844
Size: 1102 1742
NotI GC'GGCC_GC
Cuts at: 0 70 2844
Size: 70 2774
NsiI A_TGCA'T
Cuts at: 0 1581 2844
Size: 1581 1263
NspI r_CATG'y
Cuts at: 0 41 999 2765 2844
Size: 41 958 1766 79
Fragments arranged by size:
1766 958 79 41
PciI A'CATG_T
Cuts at: 0 2761 2844
Size: 2761 83
PfoI T'CCnGG_A
Cuts at: 0 720 1149 1160 1724 2844
Size: 720 429 11 564 1120
Fragments arranged by size:
1120 720 564 429 11
PmlI CAC'GTG
Cuts at: 0 729 2844
Size: 729 2115
PpuMI rG'GwC_Cy
Cuts at: 0 62 2463 2706 2844
Size: 62 2401 243 138
Fragments arranged by size:
2401 243 138 62
PsiI TTA'TAA
Cuts at: 0 1859 2844
Size: 1859 985
PspOMI G'GGCC_C
Cuts at: 0 1184 1667 2844
Size: 1184 483 1177
Fragments arranged by size:
1184 1177 483
PstI C_TGCA'G
Cuts at: 0 1004 1334 1955 2325 2844
Size: 1004 330 621 370 519
Fragments arranged by size:
1004 621 519 370 330
PvuII CAG'CTG
Cuts at: 0 1070 2844
Size: 1070 1774
SacII CC_GC'GG
Cuts at: 0 27 76 2844
Size: 27 49 2768
Fragments arranged by size:
2768 49 27
SapI GCTCTTCn'nnn_
Cuts at: 0 1561 2844
Size: 1561 1283
ScaI AGT'ACT
Cuts at: 0 184 2844
Size: 184 2660
SfcI C'TryA_G
Cuts at: 0 1000 1330 1951 2294 2321 2844
Size: 1000 330 621 343 27 523
Fragments arranged by size:
1000 621 523 343 330 27
SmaI CCC'GGG
Cuts at: 0 134 513 1277 2844
Size: 134 379 764 1567
Fragments arranged by size:
1567 764 379 134
SmlI C'TyrA_G
Cuts at: 0 917 2844
Size: 917 1927
SphI G_CATG'C
Cuts at: 0 41 2844
Size: 41 2803
SrfI GCCC'GGGC
Cuts at: 0 134 2844
Size: 134 2710
SspI AAT'ATT
Cuts at: 0 1631 2457 2844
Size: 1631 826 387
Fragments arranged by size:
1631 826 387
StyI C'CwwG_G
Cuts at: 0 1102 1534 2712 2844
Size: 1102 432 1178 132
Fragments arranged by size:
1178 1102 432 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 568 1924 2844
Size: 568 1356 920
Fragments arranged by size:
1356 920 568
TatI w'GTAC_w
Cuts at: 0 182 1284 2844
Size: 182 1102 1560
Fragments arranged by size:
1560 1102 182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 544 576 1255 1287 2502 2534 2844
Size: 544 32 679 32 1215 32 310
Fragments arranged by size:
1215 679 544 310 32 32 32
XmaI C'CCGG_G
Cuts at: 0 132 511 1275 2844
Size: 132 379 764 1569
Fragments arranged by size:
1569 764 379 132
XmnI GAAnn'nnTTC
Cuts at: 0 1607 2844
Size: 1607 1237
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BfrBI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrDI BssSI BstEII BstYI BtgI BtsI
DraI DraIII EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI Hin4I HincII MmeI MslI MspA1I NcoI
NotI NsiI NspI PciI PfoI PmlI PpuMI PsiI
PspOMI PstI PvuII SacII SapI ScaI SfcI SmaI
SmlI SphI SrfI SspI StyI TaqII TatI TstI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BciVI BclI BglI BlpI BmgBI BmtI
BsaBI BsaWI BsiWI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DrdI EcoICRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NruI PacI PasI
PflMI PmeI PpiI PshAI PspXI PsrI PvuI RsrII
SacI SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI StuI SwaI Tth111I XbaI XcmI
XhoI ZraI