(Linear) (Six-base) MAPSORT of: KIEE2307.seq Check: 4116 from: 1 to: 906 KIEE2307.orf With 182 enzymes: SgfI * November 16, 2010 13:28 .. AccI GT'mk_AC Cuts at: 0 426 906 Size: 426 480 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 757 906 Size: 757 149 AfeI AGC'GCT Cuts at: 0 826 906 Size: 826 80 AflIII A'CryG_T Cuts at: 0 609 906 Size: 609 297 AlwNI CAG_nnn'CTG Cuts at: 0 586 906 Size: 586 320 ApoI r'AATT_y Cuts at: 0 280 906 Size: 280 626 BanI G'GyrC_C Cuts at: 0 362 906 Size: 362 544 BanII G_rGCy'C Cuts at: 0 687 906 Size: 687 219 BbvCI CC'TCA_GC Cuts at: 0 726 906 Size: 726 180 BciVI GTATCCnnnnn_n' Cuts at: 0 818 906 Size: 818 88 BclI T'GATC_A Cuts at: 0 348 906 Size: 348 558 BglII A'GATC_T Cuts at: 0 442 906 Size: 442 464 BmrI ACTGGGnnnn_n' Cuts at: 0 286 906 Size: 286 620 Bpu10I CC'TnA_GC Cuts at: 0 726 906 Size: 726 180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 507 906 Size: 507 399 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 489 519 906 Size: 489 30 387 Fragments arranged by size: 489 387 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 510 906 Size: 510 396 BseYI C'CCAG_C Cuts at: 0 680 906 Size: 680 226 Bsp1286I G_dGCh'C Cuts at: 0 687 906 Size: 687 219 BssSI C'ACGA_G Cuts at: 0 41 906 Size: 41 865 BstXI CCAn_nnnn'nTGG Cuts at: 0 648 906 Size: 648 258 BstYI r'GATC_y Cuts at: 0 442 906 Size: 442 464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 387 422 906 Size: 387 35 484 Fragments arranged by size: 484 387 35 EaeI y'GGCC_r Cuts at: 0 170 650 906 Size: 170 480 256 Fragments arranged by size: 480 256 170 EarI CTCTTCn'nnn_ Cuts at: 0 399 599 906 Size: 399 200 307 Fragments arranged by size: 399 307 200 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 757 906 Size: 757 149 EcoO109I rG'GnC_Cy Cuts at: 0 736 906 Size: 736 170 EcoRI G'AATT_C Cuts at: 0 280 906 Size: 280 626 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 858 906 Size: 858 48 HaeII r_GCGC'y Cuts at: 0 828 906 Size: 828 78 MfeI C'AATT_G Cuts at: 0 548 704 906 Size: 548 156 202 Fragments arranged by size: 548 202 156 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 422 906 Size: 422 484 MscI TGG'CCA Cuts at: 0 172 652 906 Size: 172 480 254 Fragments arranged by size: 480 254 172 NspI r_CATG'y Cuts at: 0 613 906 Size: 613 293 PciI A'CATG_T Cuts at: 0 609 906 Size: 609 297 PfoI T'CCnGG_A Cuts at: 0 375 906 Size: 375 531 PpuMI rG'GwC_Cy Cuts at: 0 736 906 Size: 736 170 PshAI GACnn'nnGTC Cuts at: 0 388 906 Size: 388 518 SapI GCTCTTCn'nnn_ Cuts at: 0 599 906 Size: 599 307 SfcI C'TryA_G Cuts at: 0 587 906 Size: 587 319 SmlI C'TyrA_G Cuts at: 0 522 906 Size: 522 384 SspI AAT'ATT Cuts at: 0 324 851 906 Size: 324 527 55 Fragments arranged by size: 527 324 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 881 906 Size: 881 25 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 373 906 Size: 373 533 Enzymes that do cut: AccI AcuI AfeI AflIII AlwNI ApoI BanI BanII BbvCI BciVI BclI BglII BmrI Bpu10I BpuEI BsaXI BseRI BseYI Bsp1286I BssSI BstXI BstYI CspCI EaeI EarI Eco57MI EcoO109I EcoRI FalI HaeII MfeI MmeI MscI NspI PciI PfoI PpuMI PshAI SapI SfcI SmlI SspI TaqII XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MluI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI StyI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI