Restriction map for
pF1KE2311
(Linear) (Six-base) MAPSORT of: KIEE2311.seq Check: 1218 from: 1 to: 5463
KIEE2311.orf
With 182 enzymes: SgfI *
January 5, 2011 13:33 ..
Acc65I G'GTAC_C
Cuts at: 0 3400 3469 5463
Size: 3400 69 1994
Fragments arranged by size:
3400 1994 69
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 2438 3198 3410 4556 5369 5463
Size: 772 1666 760 212 1146 813 94
Fragments arranged by size:
1666 1146 813 772 760 212 94
AflII C'TTAA_G
Cuts at: 0 660 5463
Size: 660 4803
AleI CACnn'nnGTG
Cuts at: 0 831 3595 5463
Size: 831 2764 1868
Fragments arranged by size:
2764 1868 831
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4237 5463
Size: 4237 1226
AlwNI CAG_nnn'CTG
Cuts at: 0 102 186 5463
Size: 102 84 5277
Fragments arranged by size:
5277 102 84
ApaLI G'TGCA_C
Cuts at: 0 770 5463
Size: 770 4693
ApoI r'AATT_y
Cuts at: 0 266 533 937 1353 3748 4638 5463
Size: 266 267 404 416 2395 890 825
Fragments arranged by size:
2395 890 825 416 404 267 266
AseI AT'TA_AT
Cuts at: 0 2582 5463
Size: 2582 2881
AvaI C'yCGr_G
Cuts at: 0 2176 5463
Size: 2176 3287
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3265 3298 3392 3425 5463
Size: 3265 33 94 33 2038
Fragments arranged by size:
3265 2038 94 33 33
BanI G'GyrC_C
Cuts at: 0 3400 3469 5463
Size: 3400 69 1994
Fragments arranged by size:
3400 1994 69
BanII G_rGCy'C
Cuts at: 0 44 3939 5463
Size: 44 3895 1524
Fragments arranged by size:
3895 1524 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 318 399 751 5167 5384 5463
Size: 318 81 352 4416 217 79
Fragments arranged by size:
4416 352 318 217 81 79
BbvCI CC'TCA_GC
Cuts at: 0 3543 5463
Size: 3543 1920
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2455 2489 5463
Size: 2455 34 2974
Fragments arranged by size:
2974 2455 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 435 1120 5463
Size: 435 685 4343
Fragments arranged by size:
4343 685 435
BclI T'GATC_A
Cuts at: 0 216 5463
Size: 216 5247
BfrBI ATG'CAT
Cuts at: 0 525 4606 5463
Size: 525 4081 857
Fragments arranged by size:
4081 857 525
BglII A'GATC_T
Cuts at: 0 2395 4035 5181 5463
Size: 2395 1640 1146 282
Fragments arranged by size:
2395 1640 1146 282
Bme1580I G_kGCm'C
Cuts at: 0 774 5463
Size: 774 4689
BmrI ACTGGGnnnn_n'
Cuts at: 0 875 3933 5463
Size: 875 3058 1530
Fragments arranged by size:
3058 1530 875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5361 5463
Size: 5361 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 980 2529 5463
Size: 305 675 1549 2934
Fragments arranged by size:
2934 1549 675 305
Bpu10I CC'TnA_GC
Cuts at: 0 95 3543 5463
Size: 95 3448 1920
Fragments arranged by size:
3448 1920 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 3180 3298 5463
Size: 502 2678 118 2165
Fragments arranged by size:
2678 2165 502 118
BsaAI yAC'GTr
Cuts at: 0 1168 3928 5463
Size: 1168 2760 1535
Fragments arranged by size:
2760 1535 1168
BsaBI GATnn'nnATC
Cuts at: 0 855 5180 5463
Size: 855 4325 283
Fragments arranged by size:
4325 855 283
BsaHI Gr'CG_yC
Cuts at: 0 4789 5463
Size: 4789 674
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1508 1855 5368 5463
Size: 1508 347 3513 95
Fragments arranged by size:
3513 1508 347 95
BseYI C'CCAG_C
Cuts at: 0 430 3317 4114 4400 5463
Size: 430 2887 797 286 1063
Fragments arranged by size:
2887 1063 797 430 286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 855 3450 5463
Size: 855 2595 2013
Fragments arranged by size:
2595 2013 855
BsiEI CG_ry'CG
Cuts at: 0 37 5463
Size: 37 5426
BsiHKAI G_wGCw'C
Cuts at: 0 44 774 5463
Size: 44 730 4689
Fragments arranged by size:
4689 730 44
BsmI GAATG_Cn'
Cuts at: 0 4429 5463
Size: 4429 1034
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1962 5463
Size: 1962 3501
Bsp1286I G_dGCh'C
Cuts at: 0 44 774 3939 5463
Size: 44 730 3165 1524
Fragments arranged by size:
3165 1524 730 44
BspHI T'CATG_A
Cuts at: 0 1392 1458 2328 5463
Size: 1392 66 870 3135
Fragments arranged by size:
3135 1392 870 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 939 1975 5463
Size: 939 1036 3488
Fragments arranged by size:
3488 1036 939
BsrBI CCG'CTC
Cuts at: 0 2139 5463
Size: 2139 3324
BsrDI GCAATG_nn'
Cuts at: 0 434 5463
Size: 434 5029
BsrFI r'CCGG_y
Cuts at: 0 4029 5463
Size: 4029 1434
BstBI TT'CG_AA
Cuts at: 0 762 4512 5375 5463
Size: 762 3750 863 88
Fragments arranged by size:
3750 863 762 88
BstEII G'GTnAC_C
Cuts at: 0 1783 3205 5463
Size: 1783 1422 2258
Fragments arranged by size:
2258 1783 1422
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1540 2831 5463
Size: 1540 1291 2632
Fragments arranged by size:
2632 1540 1291
BstYI r'GATC_y
Cuts at: 0 364 1482 2142 2343 2395 3078 3564 3612
Size: 364 1118 660 201 52 683 486 48
Cuts at: 3612 4035 4388 4539 5181 5463
Size: 423 353 151 642 282
Fragments arranged by size:
1118 683 660 642 486 423 364 353
282 201 151 52 48
Bsu36I CC'TnA_GG
Cuts at: 0 1875 5463
Size: 1875 3588
BtgI C'CryG_G
Cuts at: 0 2974 3560 4878 5463
Size: 2974 586 1318 585
Fragments arranged by size:
2974 1318 586 585
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 39 3207 5463
Size: 39 3168 2256
Fragments arranged by size:
3168 2256 39
BtsI GCAGTG_nn'
Cuts at: 0 509 843 5463
Size: 509 334 4620
Fragments arranged by size:
4620 509 334
DraI TTT'AAA
Cuts at: 0 1182 5463
Size: 1182 4281
DrdI GACnn_nn'nnGTC
Cuts at: 0 2253 5463
Size: 2253 3210
EaeI y'GGCC_r
Cuts at: 0 116 1687 1821 4875 5463
Size: 116 1571 134 3054 588
Fragments arranged by size:
3054 1571 588 134 116
EarI CTCTTCn'nnn_
Cuts at: 0 257 1182 1192 2255 2402 2423 2498 3275
Size: 257 925 10 1063 147 21 75 777
Cuts at: 3275 3851 4289 4900 5255 5345 5463
Size: 576 438 611 355 90 118
Fragments arranged by size:
1063 925 777 611 576 438 355 257
147 118 90 75 21 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3119 5463
Size: 3119 2344
EcoICRI GAG'CTC
Cuts at: 0 42 5463
Size: 42 5421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 772 980 2438 2529 3198 3410 4556
Size: 305 467 208 1458 91 669 212 1146
Cuts at: 4556 5369 5463
Size: 813 94
Fragments arranged by size:
1458 1146 813 669 467 305 212 208
94 91
EcoO109I rG'GnC_Cy
Cuts at: 0 173 247 4912 5463
Size: 173 74 4665 551
Fragments arranged by size:
4665 551 173 74
EcoRI G'AATT_C
Cuts at: 0 533 1353 4638 5463
Size: 533 820 3285 825
Fragments arranged by size:
3285 825 820 533
EcoRV GAT'ATC
Cuts at: 0 853 1804 5463
Size: 853 951 3659
Fragments arranged by size:
3659 951 853
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 335 5342 5463
Size: 335 5007 121
Fragments arranged by size:
5007 335 121
HaeII r_GCGC'y
Cuts at: 0 951 5463
Size: 951 4512
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 26 51 58 1057 1089 1644 1676
Size: 19 7 25 7 999 32 555 32
Cuts at: 1676 3418 3425 3450 3457 3526 3558 4261 4293
Size: 1742 7 25 7 69 32 703 32
Cuts at: 4293 4546 4578 5226 5258 5402 5434 5463
Size: 253 32 648 32 144 32 29
Fragments arranged by size:
1742 999 703 648 555 253 144 69
32 32 32 32 32 32 32 29
25 25 19 7 7 7 7
HincII GTy'rAC
Cuts at: 0 414 1104 1369 5429 5463
Size: 414 690 265 4060 34
Fragments arranged by size:
4060 690 414 265 34
HindIII A'AGCT_T
Cuts at: 0 4658 5463
Size: 4658 805
HpaI GTT'AAC
Cuts at: 0 414 5463
Size: 414 5049
KpnI G_GTAC'C
Cuts at: 0 3404 3473 5463
Size: 3404 69 1990
Fragments arranged by size:
3404 1990 69
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1145 1190 2925 3631 5463
Size: 520 625 45 1735 706 1832
Fragments arranged by size:
1832 1735 706 625 520 45
MscI TGG'CCA
Cuts at: 0 4877 5463
Size: 4877 586
MslI CAynn'nnrTG
Cuts at: 0 520 831 1270 1397 2327 2522 2829 3595
Size: 520 311 439 127 930 195 307 766
Cuts at: 3595 4078 5463
Size: 483 1385
Fragments arranged by size:
1385 930 766 520 483 439 311 307
195 127
MspA1I CmG'CkG
Cuts at: 0 724 2188 4576 4813 5367 5463
Size: 724 1464 2388 237 554 96
Fragments arranged by size:
2388 1464 724 554 237 96
NcoI C'CATG_G
Cuts at: 0 2974 4878 5463
Size: 2974 1904 585
Fragments arranged by size:
2974 1904 585
NsiI A_TGCA'T
Cuts at: 0 527 4608 5463
Size: 527 4081 855
Fragments arranged by size:
4081 855 527
NspI r_CATG'y
Cuts at: 0 352 673 1592 5075 5463
Size: 352 321 919 3483 388
Fragments arranged by size:
3483 919 388 352 321
PasI CC'CwG_GG
Cuts at: 0 3479 5463
Size: 3479 1984
PflMI CCAn_nnn'nTGG
Cuts at: 0 186 5463
Size: 186 5277
PfoI T'CCnGG_A
Cuts at: 0 65 282 2339 5463
Size: 65 217 2057 3124
Fragments arranged by size:
3124 2057 217 65
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3873 3905 5463
Size: 3873 32 1558
Fragments arranged by size:
3873 1558 32
PpuMI rG'GwC_Cy
Cuts at: 0 173 247 4912 5463
Size: 173 74 4665 551
Fragments arranged by size:
4665 551 173 74
PshAI GACnn'nnGTC
Cuts at: 0 546 5463
Size: 546 4917
PsiI TTA'TAA
Cuts at: 0 1318 3778 3797 3815 4274 5463
Size: 1318 2460 19 18 459 1189
Fragments arranged by size:
2460 1318 1189 459 19 18
PstI C_TGCA'G
Cuts at: 0 504 1326 2591 5463
Size: 504 822 1265 2872
Fragments arranged by size:
2872 1265 822 504
PvuI CG_AT'CG
Cuts at: 0 37 5463
Size: 37 5426
PvuII CAG'CTG
Cuts at: 0 724 2188 4576 4813 5367 5463
Size: 724 1464 2388 237 554 96
Fragments arranged by size:
2388 1464 724 554 237 96
SacI G_AGCT'C
Cuts at: 0 44 5463
Size: 44 5419
SapI GCTCTTCn'nnn_
Cuts at: 0 1192 4289 5463
Size: 1192 3097 1174
Fragments arranged by size:
3097 1192 1174
ScaI AGT'ACT
Cuts at: 0 5057 5463
Size: 5057 406
SfcI C'TryA_G
Cuts at: 0 500 817 1322 2587 4056 4996 5246 5463
Size: 500 317 505 1265 1469 940 250 217
Fragments arranged by size:
1469 1265 940 505 500 317 250 217
SmlI C'TyrA_G
Cuts at: 0 481 660 3159 3277 5463
Size: 481 179 2499 118 2186
Fragments arranged by size:
2499 2186 481 179 118
SnaBI TAC'GTA
Cuts at: 0 3928 5463
Size: 3928 1535
SpeI A'CTAG_T
Cuts at: 0 1126 5463
Size: 1126 4337
SphI G_CATG'C
Cuts at: 0 1592 5463
Size: 1592 3871
StuI AGG'CCT
Cuts at: 0 20 5463
Size: 20 5443
StyI C'CwwG_G
Cuts at: 0 1533 2974 3349 4878 5463
Size: 1533 1441 375 1529 585
Fragments arranged by size:
1533 1529 1441 585 375
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 444 2869 5463
Size: 444 2425 2594
Fragments arranged by size:
2594 2425 444
TatI w'GTAC_w
Cuts at: 0 288 557 3619 4776 5055 5463
Size: 288 269 3062 1157 279 408
Fragments arranged by size:
3062 1157 408 288 279 269
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3189 3221 5463
Size: 3189 32 2242
Fragments arranged by size:
3189 2242 32
Tth111I GACn'n_nGTC
Cuts at: 0 80 5463
Size: 80 5383
XbaI T'CTAG_A
Cuts at: 0 3075 5463
Size: 3075 2388
XmnI GAAnn'nnTTC
Cuts at: 0 3269 5057 5463
Size: 3269 1788 406
Fragments arranged by size:
3269 1788 406
Enzymes that do cut:
Acc65I AcuI AflII AleI AlfI AlwNI ApaLI ApoI
AseI AvaI BaeI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII HpaI KpnI MmeI MscI MslI MspA1I NcoI
NsiI NspI PasI PflMI PfoI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SapI ScaI SfcI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AloI ApaI AscI AsiSI AvrII BamHI BbeI BglI
BlpI BmgBI BmtI BsaI BsaWI BsaXI BsiWI BspEI
BsrGI BssHII BssSI BstAPI BstZ17I ClaI CspCI DraIII
EagI EcoNI FseI FspI FspAI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PciI PmeI PmlI PspOMI PspXI PsrI RsrII SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SrfI SspI SwaI XcmI XhoI XmaI ZraI