Restriction map for
pF1KE2327
(Linear) (Six-base) MAPSORT of: KIEE2327.seq Check: 9619 from: 1 to: 2937
KIEE2327.orf
With 182 enzymes: SgfI *
February 11, 2011 17:15 ..
AccI GT'mk_AC
Cuts at: 0 2813 2937
Size: 2813 124
AclI AA'CG_TT
Cuts at: 0 1323 2937
Size: 1323 1614
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 931 2937
Size: 398 533 2006
Fragments arranged by size:
2006 533 398
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2151 2937
Size: 2151 786
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 897 929 2937
Size: 897 32 2008
Fragments arranged by size:
2008 897 32
AlwNI CAG_nnn'CTG
Cuts at: 0 294 2292 2840 2937
Size: 294 1998 548 97
Fragments arranged by size:
1998 548 294 97
ApoI r'AATT_y
Cuts at: 0 297 651 878 1416 1552 1889 2382 2447
Size: 297 354 227 538 136 337 493 65
Cuts at: 2447 2457 2937
Size: 10 480
Fragments arranged by size:
538 493 480 354 337 297 227 136
65 10
AseI AT'TA_AT
Cuts at: 0 1972 2937
Size: 1972 965
AvaI C'yCGr_G
Cuts at: 0 160 2937
Size: 160 2777
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 365 398 2937
Size: 365 33 2539
Fragments arranged by size:
2539 365 33
BanI G'GyrC_C
Cuts at: 0 164 2937
Size: 164 2773
BbsI GAAGACnn'nnnn_
Cuts at: 0 1855 2264 2937
Size: 1855 409 673
Fragments arranged by size:
1855 673 409
BciVI GTATCCnnnnn_n'
Cuts at: 0 361 2937
Size: 361 2576
BclI T'GATC_A
Cuts at: 0 2870 2937
Size: 2870 67
BlpI GC'TnA_GC
Cuts at: 0 2318 2937
Size: 2318 619
Bme1580I G_kGCm'C
Cuts at: 0 167 2937
Size: 167 2770
BmgBI CAC'GTC
Cuts at: 0 2227 2937
Size: 2227 710
BmrI ACTGGGnnnn_n'
Cuts at: 0 33 2143 2212 2937
Size: 33 2110 69 725
Fragments arranged by size:
2110 725 69 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 940 2711 2937
Size: 871 69 1771 226
Fragments arranged by size:
1771 871 226 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 2937
Size: 698 2239
BsaBI GATnn'nnATC
Cuts at: 0 665 1874 2681 2937
Size: 665 1209 807 256
Fragments arranged by size:
1209 807 665 256
BsaWI w'CCGG_w
Cuts at: 0 52 106 1173 2937
Size: 52 54 1067 1764
Fragments arranged by size:
1764 1067 54 52
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 88 118 2937
Size: 88 30 2819
Fragments arranged by size:
2819 88 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 125 2937
Size: 125 2812
BseYI C'CCAG_C
Cuts at: 0 1490 2207 2255 2937
Size: 1490 717 48 682
Fragments arranged by size:
1490 717 682 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2601 2864 2937
Size: 2601 263 73
Fragments arranged by size:
2601 263 73
BsiHKAI G_wGCw'C
Cuts at: 0 2130 2937
Size: 2130 807
BsmI GAATG_Cn'
Cuts at: 0 638 1113 2937
Size: 638 475 1824
Fragments arranged by size:
1824 638 475
BsmBI CGTCTCn'nnnn_
Cuts at: 0 889 1714 2937
Size: 889 825 1223
Fragments arranged by size:
1223 889 825
Bsp1286I G_dGCh'C
Cuts at: 0 167 2130 2937
Size: 167 1963 807
Fragments arranged by size:
1963 807 167
BspEI T'CCGG_A
Cuts at: 0 106 2937
Size: 106 2831
BsrDI GCAATG_nn'
Cuts at: 0 1655 1827 2937
Size: 1655 172 1110
Fragments arranged by size:
1655 1110 172
BsrFI r'CCGG_y
Cuts at: 0 152 2937
Size: 152 2785
BsrGI T'GTAC_A
Cuts at: 0 285 928 2937
Size: 285 643 2009
Fragments arranged by size:
2009 643 285
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2292 2737 2937
Size: 2292 445 200
Fragments arranged by size:
2292 445 200
BstEII G'GTnAC_C
Cuts at: 0 94 2937
Size: 94 2843
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2207 2937
Size: 2207 730
BstYI r'GATC_y
Cuts at: 0 760 2184 2364 2937
Size: 760 1424 180 573
Fragments arranged by size:
1424 760 573 180
Bsu36I CC'TnA_GG
Cuts at: 0 356 2937
Size: 356 2581
BtsI GCAGTG_nn'
Cuts at: 0 861 2003 2281 2937
Size: 861 1142 278 656
Fragments arranged by size:
1142 861 656 278
DraI TTT'AAA
Cuts at: 0 1542 1611 2937
Size: 1542 69 1326
Fragments arranged by size:
1542 1326 69
EaeI y'GGCC_r
Cuts at: 0 1327 2937
Size: 1327 1610
EarI CTCTTCn'nnn_
Cuts at: 0 1451 1806 2207 2937
Size: 1451 355 401 730
Fragments arranged by size:
1451 730 401 355
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 140 2897 2937
Size: 140 2757 40
Fragments arranged by size:
2757 140 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 871 931 940 2711 2937
Size: 398 473 60 9 1771 226
Fragments arranged by size:
1771 473 398 226 60 9
EcoO109I rG'GnC_Cy
Cuts at: 0 40 2937
Size: 40 2897
EcoRI G'AATT_C
Cuts at: 0 297 2937
Size: 297 2640
EcoRV GAT'ATC
Cuts at: 0 341 874 2937
Size: 341 533 2063
Fragments arranged by size:
2063 533 341
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 1785 1817 1969 2001 2356 2388
Size: 310 32 1443 32 152 32 355 32
Cuts at: 2388 2784 2815 2816 2847 2937
Size: 396 31 1 31 90
Fragments arranged by size:
1443 396 355 310 152 90 32 32
32 32 31 31 1
HincII GTy'rAC
Cuts at: 0 1593 2937
Size: 1593 1344
HpaI GTT'AAC
Cuts at: 0 1593 2937
Size: 1593 1344
MscI TGG'CCA
Cuts at: 0 1329 2937
Size: 1329 1608
MslI CAynn'nnrTG
Cuts at: 0 260 2937
Size: 260 2677
MspA1I CmG'CkG
Cuts at: 0 1762 2937
Size: 1762 1175
NaeI GCC'GGC
Cuts at: 0 154 2937
Size: 154 2783
NgoMIV G'CCGG_C
Cuts at: 0 152 2937
Size: 152 2785
NspI r_CATG'y
Cuts at: 0 192 1279 2937
Size: 192 1087 1658
Fragments arranged by size:
1658 1087 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 2104 2937
Size: 2104 833
PfoI T'CCnGG_A
Cuts at: 0 425 2918 2937
Size: 425 2493 19
Fragments arranged by size:
2493 425 19
PpuMI rG'GwC_Cy
Cuts at: 0 40 2937
Size: 40 2897
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 269 301 1904 1936 2937
Size: 269 32 1603 32 1001
Fragments arranged by size:
1603 1001 269 32 32
PstI C_TGCA'G
Cuts at: 0 834 1025 2288 2937
Size: 834 191 1263 649
Fragments arranged by size:
1263 834 649 191
SanDI GG'GwC_CC
Cuts at: 0 40 2937
Size: 40 2897
ScaI AGT'ACT
Cuts at: 0 867 1371 2153 2849 2937
Size: 867 504 782 696 88
Fragments arranged by size:
867 782 696 504 88
SexAI A'CCwGG_T
Cuts at: 0 1158 1821 2937
Size: 1158 663 1116
Fragments arranged by size:
1158 1116 663
SfcI C'TryA_G
Cuts at: 0 248 830 1021 1373 1586 2284 2699 2810
Size: 248 582 191 352 213 698 415 111
Cuts at: 2810 2937
Size: 127
Fragments arranged by size:
698 582 415 352 248 213 191 127
111
SmaI CCC'GGG
Cuts at: 0 162 2937
Size: 162 2775
SmlI C'TyrA_G
Cuts at: 0 713 2937
Size: 713 2224
SspI AAT'ATT
Cuts at: 0 1088 1129 2263 2937
Size: 1088 41 1134 674
Fragments arranged by size:
1134 1088 674 41
StyI C'CwwG_G
Cuts at: 0 901 2937
Size: 901 2036
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 506 2937
Size: 506 2431
TatI w'GTAC_w
Cuts at: 0 252 285 781 865 928 1369 1750 2151
Size: 252 33 496 84 63 441 381 401
Cuts at: 2151 2847 2937
Size: 696 90
Fragments arranged by size:
696 496 441 401 381 252 90 84
63 33
XbaI T'CTAG_A
Cuts at: 0 1866 2937
Size: 1866 1071
XmaI C'CCGG_G
Cuts at: 0 160 2937
Size: 160 2777
XmnI GAAnn'nnTTC
Cuts at: 0 1359 2264 2937
Size: 1359 905 673
Fragments arranged by size:
1359 905 673
Enzymes that do cut:
AccI AclI AcuI AlfI AloI AlwNI ApoI AseI
AvaI BaeI BanI BbsI BciVI BclI BlpI Bme1580I
BmgBI BmrI BpmI BpuEI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrDI
BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtsI
DraI EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
Hin4I HincII HpaI MscI MslI MspA1I NaeI NgoMIV
NspI PflMI PfoI PpuMI PsrI PstI SanDI ScaI
SexAI SfcI SmaI SmlI SspI StyI TaqII TatI
XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AleI ApaI ApaLI AscI AsiSI AvrII BamHI BanII
BbeI BbvCI BcgI BfrBI BglI BglII BmtI BplI
Bpu10I BsaI BsaAI BsaHI BsiEI BsiWI BspHI BspMI
BsrBI BssHII BssSI BstBI BstZ17I BtgI BtgZI ClaI
CspCI DraIII DrdI EagI EcoICRI EcoNI FalI FseI
FspI FspAI HaeII HindIII KasI KpnI MfeI MluI
MmeI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XcmI XhoI
ZraI